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Citations for Takara Bio :
1 - 50 of 828 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 10 µl of 2x MightyAmp Buffer (Takara Bio Inc., Shiga, Japan), and 7.8 µl of nuclease-free water ...
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bioRxiv - Microbiology 2020Quote: ... in buffer solution (10xLow salt buffer, Takara Bio Co. Ltd.) and 5 folds dilution by de-ionized water ...
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bioRxiv - Immunology 2021Quote: ... PCR was performed with 2x SeqAMP buffer and SeqAmp DNA polymerase (Takara USA) and for the final amplification ...
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bioRxiv - Immunology 2021Quote: ... PCR was performed with 2x SeqAMP buffer and SeqAmp DNA polymerase (Takara USA) and for the final amplification ...
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bioRxiv - Microbiology 2021Quote: ... in solution and stopped by adding guanidinium thiocyanate solution buffer NTI (Takara Bio). After the methylation reaction following DNA purification ...
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bioRxiv - Neuroscience 2024Quote: ... samples in homogenization buffer (Clontech, Cat: 2313A), were thawed on ice ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were collected and mixed with Laemmli buffer after measuring the protein concentration using a TaKaRa BCA Protein Assay Kit (TaKaRa). Samples were subjected to SDS-polyacrylamide gel electrophoresis and electrotransferred onto polyvinylidene difluoride membranes ...
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bioRxiv - Cancer Biology 2023Quote: For indexing PCR, 78μL PCR mix (24μL H20, 50μL 2X SeqAmp CB PCR buffer (Takara Cat. #638526), 2μL SeqAmp DNA polymerase ((Takara Cat ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were subsequently mixed with 10X Loading Buffer (Takara) and resolved on agarose gels using GelRed Nucleic Acid Gel Stain (Biotium ...
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bioRxiv - Microbiology 2020Quote: ... or Premix ExTaq 2x (Takara) reagents and the StepOne Plus (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... Takara Emerald (2x;Takara Co.) was used for RT–sqPCR ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA amplification was performed by adding 15 μL of Seq amp PCR mixture (12.5 μL of 2x SeqAmp buffer (Takara Bio), 0.05 μL of 100 μM N-IS PCR primer ...
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bioRxiv - Plant Biology 2023Quote: ... PrimeSTAR Max Premix (2x) (TaKaRa, Japan) was used for PCR with the degenerate primers listed in Supplemental Table S10 ...
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bioRxiv - Genomics 2023Quote: ... 12.5µL 2X CloneAmp Pre-Mix (Takara) and 1µL each 10µM of tpdel-F (5’-TGCTCTCGCATGGCTATGTT-3’ ...
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bioRxiv - Neuroscience 2022Quote: ... where the lysing solution is composed of single-cell lysis buffer (Takara #635013): 1x RNase Inhibitor Murine (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2x Titanium Taq DNA Polymerase (Takara Bio.); 100 ng BSA per ul ...
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bioRxiv - Cell Biology 2023Quote: ... and ligated using 2x Ligation Mix (Takara) and checked for insert orientation ...
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bioRxiv - Pathology 2021Quote: ... using 2x SYBR-green mix (Takara Bio, Clontech, USA) in an Applied Biosystems® QuantStudio(tm ...
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bioRxiv - Pathology 2021Quote: ... using 2x SYBR-green mix (Takara Bio, Clontech, USA) in an Applied Biosystems® QuantStudio(tm ...
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bioRxiv - Microbiology 2024Quote: ... containing 25 μl 2x Premix Taq (Takara Bio, China), 1 μl each primer(10 μM ...
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bioRxiv - Microbiology 2020Quote: ... Real-time PCR was performed with 2x SYBR mix (Takara) in a QuantStudio-6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Synthetic Biology 2020Quote: ... and Q5 master mix or 2X PrimeSTAR Max Premix (Takara) in a 20 µL PCR reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR mixture containing 10 µL Emerald Master mix (2X) (TaKaRa), 2 µL total genomic DNA (10-15 ng/µL) ...
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bioRxiv - Genomics 2022Quote: ... For PrimeSTAR GXL Premix (2x, Takara Bio, cat# R051A, Japan), a 50 μl -reaction system was prepared as follows ...
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bioRxiv - Microbiology 2022Quote: ... 12.5 μl Takara EmeraldAmp Master Mix 2x (Takara Bio, Japan), 0.5 μl primers rbcL40+ and rbcL1444- (0.2 μM final concentration) ...
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bioRxiv - Microbiology 2024Quote: ... The harvested tissues and the fecal samples were collected in protein lysis buffer and TRIzol (Takara) for protein and RNA extraction respectively and were stored at -80°C ...
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bioRxiv - Genomics 2022Quote: ... 25 μl PrimeSTAR GXL Premix (2x, Takara Bio, cat# R051A, Japan), 20 ng DNA and NF water to a total volume of 50 μl ...
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bioRxiv - Microbiology 2024Quote: ... 10 μl 2x EmeraldAmp® GT PCR Master Mix (Takara Bio), 8 μl ddH2O ...
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bioRxiv - Microbiology 2023Quote: ... A 2x hot-start PCR master mix from Takara (cat#: R405A) was used for high-fidelity PCR amplification of the pBCG02 backbone ...
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bioRxiv - Microbiology 2024Quote: ... 10 μl of 2x EmeraldAmp® GT PCR Master Mix (Takara Bio), 6 μl of ddH2O ...
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bioRxiv - Microbiology 2024Quote: ... we used Emerald Amp MAX PCR Master Mix (2X) (Takara Bio, USA). All the primer sets were manufactured by Macrogen ...
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bioRxiv - Microbiology 2024Quote: ... comprising 25 µl of 2x probe qPCR mix (Takara Bio Inc., Otsu, Japan), 0.2 µl each of forward and backward primers (100 µmol l-1 each ...
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bioRxiv - Cell Biology 2024Quote: ... semiquantitative PCR was performed using 2X EmeraldAmp GT PCR Master Mix (Takara-#RR310) and gene-specific primers and the amplified PCR products were visualized on 2% agarose gel ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative PCR reaction was conducted with TB Green Advantage qPCR 2X- premix (Takara) using a Bio-Rad CFX Connect Real-Time PCR Detection System ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from 250 µl of EDTA-anticoagulated venous blood samples collected on-site using RNAiso Blood solution (Cat# 9112; Takara Bio, Japan) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... Silk glands and whole-body samples from which silk glands had been removed were rinsed well with PBS buffer (pH 7.4) (Takara Bio) before imaging to remove fluorescent hemolymph ...
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bioRxiv - Immunology 2023Quote: ... The RT reaction mixture was prepared on ice and contained (per sample): 4 µL SmartScribe 1st Strand 5X buffer (Takara), 2 µL 100 µM DTT (Takara) ...
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bioRxiv - Zoology 2022Quote: ... and 2X KAPA HiFi Hot Start Ready Mix 15 μl (TaKaRa Bio Inc., Japan), via a procedure with a thermal instrument (Applied Biosystems 9700 ...
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bioRxiv - Systems Biology 2022Quote: ... and 2X KAPA HiFi Hot Start Ready Mix 15 μl (TaKaRa Bio Inc., Japan), via a two-stage PCR procedure with a thermal instrument (Applied Biosystems 9700 ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 µl/well of beads were washed twice with TE solution and then resuspended in lambda DNA solution (diluted to approximately 20 ng/µL in TE solution, 3010, Takara). Then ...
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bioRxiv - Plant Biology 2023Quote: ... each egg cell sample was transferred to a lysis buffer supplied in the SMART-Seq HT kit (Takara Bio, Shiga, Japan). The lysates were immediately used for cDNA synthesis or frozen in liquid nitrogen and stored at -80 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR was performed according to the the manuals of PrimeSTAR Max Premix (2X) (TaKaRa, R045) or 2×EasyTaq PCR SuperMix (+ dye ...
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bioRxiv - Molecular Biology 2022Quote: 7.5 ng cDNA were mixed with 2X TB green Premix Ex Taq II (Takara, RR820B), 10μM forward and reverse primers ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative PCR was performed with 2x SYBR green mix (#RR420L, Takara Bio, Otsu, Shiga, Japan) and primers against JAK1 ...
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bioRxiv - Genomics 2023Quote: Specimens were run in technical duplicates for tprK deep sequencing using 10.5µL DNA added to 12.5µL 2X CloneAmp Pre-mix (Takara), 1µL 10µM barcoded tprK-F and 1µL 10µM barcoded tprK-R (Table S2 in Supplemental Data) ...
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bioRxiv - Neuroscience 2024Quote: ... and annealed before being ligated into the cut vector using 2x Ligation Master Mix (Takara 6023). After sequencing to confirm correct cloning ...
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bioRxiv - Biochemistry 2024Quote: Assembly inserts were produced through two sequential PCR reactions with 2X CloneAmp HiFi PCR premix (Takara). Both reactions were performed in 25 μl volumes ...
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bioRxiv - Microbiology 2024Quote: ... PCR reactions were carried out with a 2X Hot Start PCR Master Mix (TaKaRa; catalog no. R405A). A list of primers used for PCR is provided in Table S2 ...
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bioRxiv - Molecular Biology 2020Quote: ... ExTaq buffer (Clontech), dNTPs ...
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bioRxiv - Cell Biology 2021Quote: ... ExTaq buffer (Clontech), dNTPs ...