Labshake search
Citations for VWR :
1 - 50 of 827 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: Frozen cell and exosome pellets were lysed using 2X RIPA buffer (VWR) with protease inhibitor cocktail (Sigma-Aldrich ...
-
bioRxiv - Immunology 2024Quote: ... in sample buffer (1X MES VWR cat ...
-
bioRxiv - Immunology 2024Quote: ... in sample buffer (1× MES VWR cat ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were washed in 2X saline-sodium citrate (SSC) buffer containing 10% deionized formamide (VWR) and hybridized with fluorescently labeled oligonucleotide probe mixture for 4-16 hours at 37°C in hybridization buffer (2X SSC ...
-
bioRxiv - Immunology 2024Quote: ... liquid 2X glucose 1X yeast Extract (2X GYE) media (VWR, Radnor, PA, USA) was used to grow mycelia cultures in a shaking incubator at 30°C and 150 rpm for 4 days ...
-
bioRxiv - Bioengineering 2021Quote: ... HEPES buffer solution (C8H18N2O4S, VWR, 1 M in water, pH = 7.3), potassium phosphate dibasic (K2HPO4 ...
-
bioRxiv - Microbiology 2021Quote: ... HEPES buffer solution (C8H18N2O4S, VWR, 1 M in water, pH = 7.3), potassium phosphate dibasic (K2HPO4 ...
-
bioRxiv - Bioengineering 2024Quote: ... EPES buffer solution (C8H18N2O4S, VWR, 1 M in water, pH = 7.3), potassium phosphate dibasic (K2HPO4 ...
-
bioRxiv - Pathology 2023Quote: ... in a 125 mM phosphate buffer (PB) solution (VWR International, Pennsylvania, USA), pH 7.4 ...
-
bioRxiv - Neuroscience 2023Quote: ... in a 125 mM phosphate buffer (PB) solution (VWR International, Pennsylvania, USA), pH 7.4 ...
-
bioRxiv - Cancer Biology 2022Quote: ... and 10-30μg samples were prepared with a 6x reducing sample buffer containing β-mercaptoethanol (VWR, M131). Proteins were resolved by SDS-PAGE using NuPAGE 4-12% Bis-Tris precast gels (Fisher Scientfic ...
-
bioRxiv - Cell Biology 2023Quote: ... washed once with 1x phosphate buffer solution (PBS) without calcium and magnesium (VWR), and dissociated with 0.25% Trypsin-EDTA (Life Technologies ...
-
bioRxiv - Cancer Biology 2020Quote: ... under denaturating conditions (SDS PAGE Sample loading buffer (VWR, GENO786-701) supplemented with 1 mM DTT ...
-
bioRxiv - Cancer Biology 2021Quote: ... under denaturating conditions (SDS PAGE Sample loading buffer (VWR, GENO786-701) supplemented with 1 mM DTT) ...
-
bioRxiv - Cell Biology 2022Quote: ... under denaturating conditions (SDS PAGE sample loading buffer (VWR, GENO786-701) supplemented with 1 mM DTT ...
-
bioRxiv - Physiology 2024Quote: ... muscle samples were homogenized in RIPA lysis buffer (97063-270, VWR) with protease and phosphatase inhibitor cocktails (97063-010 ...
-
bioRxiv - Genetics 2024Quote: ... antibody solutions were made in blocking buffer supplemented with Tween-20 (VWR, 100216-360). The blot was incubated in the primary antibody solution while rocking at 4°C overnight ...
-
bioRxiv - Physiology 2020Quote: ... RNA probes were stored in 25% formamide/2x saline-sodium citrate (25% FA/2x SSC; VWR Chemicals, Leicestershire, UK) at −20°C for long-term storage.
-
bioRxiv - Cell Biology 2021Quote: ... Blocking buffer and primary antibody incubation solution was 5% non-fat dairy milk powder (VWR) or 5% (w/v ...
-
bioRxiv - Microbiology 2022Quote: ... Samples were mixed with 4X LDS NuPage sample buffer to a final concentration of 1X and dithiothreitol (71003-396, VWR) to a final concentration of 50 mM ...
-
bioRxiv - Microbiology 2022Quote: ... samples were harvested in SDS-lysis buffer (20 mM TrisHCl (VWR # 97061-794) pH7 ...
-
bioRxiv - Biophysics 2021Quote: ... Substrate and buffer solutions were filtered once with a 0.2 µM cellulose acetate syringe filter (VWR) before being added to the mica.
-
bioRxiv - Bioengineering 2024Quote: ... samples were incubated in DAPI solution for 15 minutes and then mounted using FluorSave (VWR, #345789).
-
bioRxiv - Immunology 2023Quote: ... All samples were diluted ten times in low EDTA TE-buffer (VWR, cat.: APLIA8569.0500) in a total volume of 20 μL ...
-
bioRxiv - Microbiology 2020Quote: ... PCR was performed with the kit TaqPolimerase 2X (VWR) using the following PCR program ...
-
bioRxiv - Microbiology 2023Quote: ... 12.5 µl of 2x KAPA HiFi HotStart mastermix (VWR) and the same PCR conditions were followed as in PCR step 1 ...
-
bioRxiv - Cell Biology 2023Quote: ... and a 2x volume −20°C ethanol (VWR Chemicals). After overnight incubation at −20°C the DNA was pelleted at 17000g for 30 min at 4°C ...
-
bioRxiv - Synthetic Biology 2024Quote: ... a standard exponential PCR (peqSTAR 2X PCR thermocycler, VWR) was used to make dsDNA template containing the coding strand and it’s complementary strand ...
-
bioRxiv - Developmental Biology 2023Quote: ... The samples were then transferred overnight in a depigmentation solution of methanol containing 6% H2O2 (VWR, 216763) at 4°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... Lysates were prepared with 6X SDS sample buffer containing 12% (v/v) β-ME (VWR) and separated on Bolt™ or NuPAGE 4-12% Bis-Tris gels (Invitrogen) ...
-
bioRxiv - Microbiology 2023Quote: ... and 12.5 µl of 2x KAPA HiFi HotStart mastermix (VWR). PCR conditions were 95ºC for 3 min ...
-
bioRxiv - Bioengineering 2023Quote: ... or RedTaq DNA Polymerase 2X Master Mix (VWR Life Science) using primers designed in Geneious Prime 2023.1.2 (Supplementary Table 5) ...
-
bioRxiv - Neuroscience 2022Quote: ... the flattened cortex was removed from the microscope slides and left in a cryoprotective dimethyl sulfoxide solution (2% dimethyl sulfoxide, DMSO, in 0.125 M phosphate buffer; VWR) overnight ...
-
bioRxiv - Neuroscience 2024Quote: ... cDNA libraries were generated by incubating the RNA samples in a solution containing qScript cDNA SuperMix (VWR #101414-102) and nuclease-free water ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... We removed nearly all dissection/fixation solution from the sample and picked up the coverslip with a HistoBond microscope slide (VWR). Samples were then frozen on dry ice ...
-
bioRxiv - Microbiology 2023Quote: Each sample was chemically disrupted with an equal volume of 1:1 solution of ethyl acetate (EtOAc, VWR HiPerSolv Chromanorm) and methanol (MeOH ...
-
bioRxiv - Microbiology 2024Quote: Each sample was chemically disrupted with an equal volume of 1:1 solution of ethyl acetate (EtOAc, VWR HiPerSolv Chromanorm) and methanol (MeOH ...
-
bioRxiv - Developmental Biology 2024Quote: Embryonic tissue samples from different embryonic days were fixed overnight at room temperature in 4% paraformaldehyde solution (VWR, Pennsylvania, USA), followed by dehydration and embedding in paraffin ...
-
bioRxiv - Immunology 2021Quote: ... 2 mL of HEPES-buffered saline pH 7.0 (2X for transfection) (VWR) was added dropwise to the previous mixture ...
-
bioRxiv - Microbiology 2024Quote: ... 12.5 µL of EconoTaq PLUS green 2X Master Mix (VWR, Radnor, PA), 1 µL of 10 µM 16S 8F primer ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were incubated for 30 minutes in permeabilization buffer (PB; 0.5% Bovine serum albumin (VWR International; 126575-10), 0.25% Fish skin gelatin (Sigma ...
-
bioRxiv - Cell Biology 2022Quote: ... fixed breast samples were impregnated with 30% Sucrose solution in 1X PBS for at least 48h and embedded in OCT (VWR, #361603E). 70 μm-thick sections were incubated with 0.5% Triton-blocking buffer (5% FBS and 2% BSA in 1X PBS ...
-
bioRxiv - Bioengineering 2022Quote: ... cells for both batches were seeded onto the material at a confluence comprised between 50% and 60% on fibronectin coated (15 μg/ml in PBS buffer solution) 18-mm round glass coverslip (VWR, Radnor, USA). The cells were maintained in incubator at 37°C and 5% CO2.
-
bioRxiv - Molecular Biology 2022Quote: ... RNApure solution (VWR), following the manufacturer’s instruction or ...
-
bioRxiv - Biophysics 2024Quote: Left ventricular samples were thawed in ice-cold Relax buffer containing 1% Triton-X 100 (VWR, CAS 9002-93-1), 0.1% saponin (Sigma Aldrich ...
-
bioRxiv - Plant Biology 2021Quote: ... Membrane protein samples containing 1 μg total Chl were solubilized and separated by electrophoresis on a 10 % SDS-polyacrylamide gel using Next Gel solutions and buffers (VWR, Radnor, PA, USA). Proteins were transferred to Immobilon-P PVDF membranes (MilliporeSigma ...
-
bioRxiv - Microbiology 2020Quote: ... a 1:1 ratio of 0.6% agarose and 2X EMEM (VWR, Cat # 10128-758) supplemented with 5% FBS ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Karyotyping PCRs were conducted using RedTaq DNA Polymerase 2X Master Mix (VWR Life Science) with the following published primers ...
-
bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was extracted from lysed samples using the Quick Extract DNA Extraction Solution (Catalog: QE09050, Bio-Search Technologies, VWR, Radnor, PA, USA), following the protocol provided by the manufacturer ...
-
bioRxiv - Genomics 2024Quote: ... the samples were air-dried for 10 min and resuspended in 200 μl of the Tris buffer pH8.0 (VWR, 97062-674), and incubated at 55°C for 10 min ...