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Citations for 3M :
1 - 50 of 345 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Supernatants were mixed with 5X Laemmli Buffer (0.3M Tris-H-Cl ...
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bioRxiv - Cancer Biology 2021Quote: ... 1000 ng and 500 ng of each RNA sample were spotted to a nylon membrane (Hybond N, Dutscher RPN203B) previously soaked in 2X SSPE solution (0.3M NaCl, 20mM sodium phosphate, 2 mM EDTA) and inserted in the dot blot apparatus (SCIE-PLAS) ...
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bioRxiv - Genomics 2024Quote: ... Beads were washed 4 times with 2x SSC buffer containing 0.1% NP40 (0.3M of NaCl ...
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bioRxiv - Microbiology 2022Quote: ... Hybridized membranes were washed 2x with 20x SSC buffer (3M NaCl, 300 mM NaCit, pH 7.0), washed 3x with 2x SSC ...
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bioRxiv - Molecular Biology 2024Quote: ... 2X PK buffer (0.2M Tris-HCl [pH 7.5], 25mM EDTA, 0.3M NaCl, 2% [w/v] SDS) was added to stop the reaction ...
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bioRxiv - Microbiology 2021Quote: ... 6.78 x 105 NHDF were resuspended in 4 ml of cold neutralization solution (2x DMEM, 3 % FCS, 3 % 3M HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were washed rapidly with PBS before incubation (at least 30 min) with pre-hybridization buffer : 15% formamide in 2X SSPE pH8.0 (0.3M NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed rapidly with PBS before incubation for at least 30min with pre-hybridization buffer (15% formamide in 2X SSPE pH8.0 [0.3M NaCl ...
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bioRxiv - Physiology 2023Quote: ... Samples were dehydrated in an increasing methanol (MeOH) gradient (20%→40%→60%→80%→(2x)100%) v/v in B1n buffer (0.3M glycine ...
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bioRxiv - Molecular Biology 2020Quote: ... frozen blood sample is thawed and lysed in NUN buffer (0.3M NaCl ...
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bioRxiv - Genetics 2024Quote: Histone samples were mixed with 5X Loading Buffer (5% SDS, 0.3M Tris pH 6.8 ...
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bioRxiv - Genomics 2024Quote: ... Beads were washed 4 times with 2x SSC buffer containing 0.1% NP40 (0.3M of NaCl, 0.3M of Sodium citrate, 0.1% NP-40) at 37°C ...
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bioRxiv - Pathology 2021Quote: ... samples were treated with the permeabilization solution (0.3M Glycine and 20% DMSO in PBS) overnight at 37°C ...
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bioRxiv - Neuroscience 2021Quote: Samples were incubated in primary antibody solution (primary antibody in 5% DMSO/0.3M Glycine/PTxwH) for 8 days ...
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bioRxiv - Neuroscience 2022Quote: ... Bleached samples were incubated in permeabilization solution (PBS with 0.2% Triton, 20% DMSO, 0.3M glycine) and then in blocking solution (PBS with 0.2% Triton ...
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bioRxiv - Immunology 2022Quote: ... Samples were resuspended in 200 µL elution buffer 1 (EB1, 50% ACN, 0.3M NH4OH) and incubated for 1 hour on a ThermoMixer at 45°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μg of total RNA dots were added to a nitrocellulose membrane previously washed with SSC 2X buffer (SSC 20X: NaCl 3M, sodium citrate 300 mM, pH 7). The membrane was incubated for 5 min at 65°C and exposed to 400 mJ/cm2 of 254 nm UV in a UV crosslinker (Biolink Shortwave 254 nm) ...
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bioRxiv - Cell Biology 2020Quote: ... 75 μl digested sample or iron standard was mixed with 75 μl ferrozine solution (1mM Ferrozine, 3M Sodium Acetate and 1% mercaptoacetic acid) ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were then washed overnight at 37◦C in permeabilisation solution (PTx.2, 0.3M glycine, 20% DMSO).
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bioRxiv - Genetics 2022Quote: Dot blot membranes were rinsed in 2X SSC (20x: 3M NaCl and 0.3M citric acid trisodium salt dihydrate ...
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bioRxiv - Biophysics 2023Quote: ... Different concentrations of protein samples were mixed with 4X Loading buffer (0.25 M Tris-HCl pH 6.8, 8% SDS, 0.3M DTT ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was washed twice with 2x SSC (diluted from 20x: 3M Sodium chloride ...
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bioRxiv - Molecular Biology 2022Quote: ... and loaded onto StageTips packed with 2x disks SDBRPS material (3M Empore). StageTips were washed 1x with 100 μL 99% ethyl acetate/1% TFA ...
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bioRxiv - Cell Biology 2020Quote: ... cells were lysed with 1 x SDS sample buffer (0.32 M Tris pH 6.8, 10% SDS, 50% glycerol, 3M β-mercaptoethanol ...
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bioRxiv - Cell Biology 2021Quote: ... and resuspended in 50mL MURB protein sample buffer (100mM MES pH7.0, 1% SDS, 3M urea, 10% 2-mercaptoethanol) prior to Western analysis.
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bioRxiv - Molecular Biology 2022Quote: 1 ml of frozen blood sample is thawed and lysed in 10 ml of the NUN buffer (0.3M NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... lysed in protein sample buffer (0.2M Tris HCl, 0.4 M DTT, 277 mM SDS, 6mM Bromophenol Blue, 4.3M glycerol), and boiled and subjected to SDS-PAGE and Western blotting as described above.
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bioRxiv - Bioengineering 2020Quote: ... samples were incubated in penetration buffer (0.2% Triton X-100, 20% DMSO, and 0.3M glycine in 1X PBS), then blocking buffer (0.2% Triton X-100 ...
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bioRxiv - Biochemistry 2023Quote: The embryo derivative samples (Embryos, in situ NuMat, and in situ NuMat extracted 1M, 2M, 3M GdnHCl buffers) were blocked by rotating in 3% BSA in PBT for 2 hours at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... Then 50 μL of each sample were mixed with 115 μL of acid molybdate solution (17.55 g/L of Ammonium molybdate tetrahydrate, 3M sulfuric acid) and 700 μL of deionized water ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... resuspended in 50 μl of sample buffer with urea (50 mM Tris-HCl, pH 6.8, 100 mM DTT, 3% SDS, 3M urea ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined with a BCA protein assay kit (23225, Thermo Fischer Scientific) and cell lysates were incubated with 5X Reducing Sample Buffer (0.3M Tris-HCL pH6.8 ...
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bioRxiv - Neuroscience 2020Quote: ... and Western Blotting samples were denatured at 94°C for 5 minutes in 3 x SDS sample buffer (150mM Tris pH 8, 6% SDS, 0.3M DTT ...
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: ... Antibodies were then eluted by incubating the samples in an elution buffer (0.5 M L-glycine, 3 M Urea, 3M Guanidium Chloride ...
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bioRxiv - Developmental Biology 2023Quote: ... in each round the sample was incubated for ten minutes at room temperature in an elution buffer (0.5M L-Glycine, 3M urea, 3M guanidine hydrochloride ...
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bioRxiv - Developmental Biology 2023Quote: ... in each round the sample was incubated for ten minutes at room temperature in an elution buffer (0.5M L-Glycine, 3M urea ...
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bioRxiv - Molecular Biology 2020Quote: Purified His6-KIAA1841(BTB) protein at 0.1mg/ml in sample buffer (10mM HEPES, pH8.0; 0.3M NaCl, 10% glycerol, 1mM TCEP) was crosslinked with 5-fold increasing concentration of glutaraldehyde (i.e ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein lysate was mixed at an equal volume with 3x SDS sample buffer (187.5 mM Tris-HCl [pH 6.8], 30% glycerol, 6% SDS, 0.3M DTT, 0.01% bromophenol blue) and boiled at 105°C for 5 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Pipette solution: (3M KCl). (B ...
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bioRxiv - Immunology 2021Quote: ... The membrane was wet with 2X SSC (0.3M NaCl, 30 mM Trisodium citrate, pH 7) and transferred to a hybridization tube ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were incubated in 2x SSC-T (3.0M NaCl, 0.3M Sodium Citrate, 0.1% Tween 20) for 5 minutes at RT ...
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bioRxiv - Microbiology 2024Quote: ... Five ml of the supernatant were then layered over a CsCl step gradient (4 ml 3M and 4 ml 5M CsCl solution in SM buffer) in 13.5 mL Quick-Seal Ultracentrifugation Tubes (Beckman Coulter) ...
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bioRxiv - Systems Biology 2020Quote: ... Blocking buffer (0.3M glycine buffer, 10% Goat Serum, 1XPBS) was added to ‘dry’ pellets on ice for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... Two in vivo samples (sample identifier D10_3M and D5_1M) sequenced on the HiSeq 2500 at the GTF were included in the MiniSeq run which confirmed that the two runs gave comparable results.
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bioRxiv - Plant Biology 2023Quote: ... 0.1 mg/ml of RNase A in 2x SSC (0.3M NaCl, 30 nM sodium citrate, pH 7.0) was added onto the coverslips and incubated at 37°C for 45 min ...
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bioRxiv - Molecular Biology 2024Quote: Precipitate RNA with 2X volume Molecular Grade Absolute ethanol and 0.1X volume Sodium Acetate (3M, pH 5.2). Incubate the samples for 1 hour at -20ºC and centrifuge at 14,000 g for 15 minutes at 4ºC.
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bioRxiv - Immunology 2021Quote: ... 50μl of stopping solution (3M NaOH) after the development of colour was added and absorbance was taken at 405nm.
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bioRxiv - Genetics 2024Quote: ... diff Solution Tablets (3M, Athlone, Ireland). The tablets were dissolved in water and ...
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bioRxiv - Molecular Biology 2024Quote: ... Recipes for buffers are as follows: Elution buffer (0.5M Glycine, 3M Urea ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was precipitated with 2X volume Molecular Grade Absolute ethanol and 0.1X volume Sodium Acetate (3M, pH 5.2). The samples were then incubated for 1 hour at -20°C and centrifuged at 14,000 g for 15 minutes at 4°C ...