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Citations for Promega :
1 - 50 of 3140 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... An equal volume of 2x PLB buffer (Promega) was added to permeabilize the yeast cells ...
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bioRxiv - Systems Biology 2024Quote: ... 12.5 μL of 2x Express Buffer (T7 RiboMAX; Promega), 2.5 μL of Express Mix (T7 RiboMAX ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and 5 μL 2x buffer for T4 DNA Ligase (Promega) were mixed and incubated at 55°C for 10 min ...
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bioRxiv - Plant Biology 2023Quote: ... and 200 μl 2x cell culture lysis buffer were added (Promega, E1531). Luciferase activity was determined by mixing 50 μl of protoplast lysate with 50 μl luciferase substrate (Promega ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 10 μL of 2X furimazine (diluted from Promega 50X stock into CETSA buffer) solution was added to the 10 μL of transfected HEK293T cells in each well ...
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bioRxiv - Molecular Biology 2022Quote: ... First, we added 20 uL 2X formamide denaturing solution (20% Glycerol, 80% Formamide (Promega # H5051), 60 mg/mL Bromophenol Blue ...
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bioRxiv - Molecular Biology 2020Quote: ... 5X PCR Flexi Buffer and 2X GoTaq PCR Master Mix were purchased from Promega. An aliquot of ABIL® EM 90 by Evonik was gifted to us from Professor Shuhuai Yao’s lab (see Acknowledgements).
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bioRxiv - Cell Biology 2022Quote: ... 2X (Promega) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... Samples were treated with RNase (RNaseA Solution, Promega) for 30 min and proteins were denatured for 10 min at 90 °C in 2× SDS loading buffer with 10 mM DTT ...
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bioRxiv - Physiology 2024Quote: ... Samples were in-solution digested with trypsin (Promega). Tryptic peptides were purified by STAGE tips and LC-MS/MS measurements were performed on a QExactive plus and an Orbitrap Exploris 480 mass spectrometer coupled to EasyLC 1200 nanoflow-HPLCs (all Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... 2X (M712, Promega) with the following protocol ...
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bioRxiv - Microbiology 2023Quote: ... Sample digestion was carried out by resuspending beads in 1 µL of trypsin solution (0.2 µg trypsin in Promega V5111 resuspension buffer) and 19 µL 50 mM ammonium bicarbonate and incubating at 37 °C with agitation for 18 hours ...
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bioRxiv - Cell Biology 2024Quote: ... samples were digested in solution first with chymotrypsin (Promega) at a ratio of 1:80 (enzyme to protein ...
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bioRxiv - Plant Biology 2024Quote: ... Luminescence was monitored after adding 50 µL of 2X treatment solution via autoinjector using the GloMax Navigator (Promega). After elicitor-induced luminescence was recorded ...
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bioRxiv - Cancer Biology 2023Quote: ... The β-Galactosidase activity was measured in 30 µl of lysate incubated with 20 µl of reporter lysis buffer and 50 µl of 2X β-gal Enzyme Assay buffer (Promega, Cat# E2000). The absorbance was read in iMark microplate reader (BioRad) ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were extracted using cell lysis buffers (Promega), luciferin substrate was added and the plates were measured for luminenescence for 8-15 minutes in a SpectraMax L 96-well plate reader (Molecular Devices).
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bioRxiv - Cell Biology 2020Quote: ... Samples were diluted in passive lysis buffer (Promega) before analysis of luciferase protein levels using Dual-Glo luciferase assay system (Promega ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were lysed in passive lysis buffer (Promega) and activity measured using a dual-luciferase reporter assay system (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... Samples were lysed in passive lysis buffer (Promega) and activity measured using a dual-luciferase reporter assay system (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: ... After two low stringency washes for 5 minutes each with 2× saline sodium citrate/ 0.1% sodium dodecyl sulfate buffer (2x SSC buffer; Promega Co., Madison, WI, Catalog# V4261) and a high stringency wash for 2 min with 1x SSC buffer ...
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bioRxiv - Genetics 2024Quote: ... in 2x SSCT (0.1% Tween-20 in 2x SSC (Promega, Cat# V4261)) for 5 min at RT ...
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bioRxiv - Neuroscience 2022Quote: ... and 2X GoTaq (Promega). Thermocycler parameters ...
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bioRxiv - Genetics 2024Quote: ... Samples were diluted 1:1 with water and again 1:1 with 2X GoTaq Master Mix (Promega M712B) containing appropriate primers and amplified in 23 PCR cycles ...
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bioRxiv - Microbiology 2023Quote: ... samples were lysed with 1x Passive Lysis Buffer (Promega) and relative luciferase activity measured using Dual luciferase Stop and Glo reagents (Promega ...
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bioRxiv - Genetics 2020Quote: ... PCR Master Mix 2x (Promega) was used ...
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bioRxiv - Microbiology 2023Quote: ... 2x GoGreen Master Mix (Promega) was used for these reactions ...
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bioRxiv - Physiology 2024Quote: ... 2x GoTaq master mix (Promega) and 1μl cDNA ...
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bioRxiv - Cancer Biology 2023Quote: ... where 20 μl of yeast culture was mixed in each well with an equal volume of 2x Passive Lysis Buffer (Promega) to permeabilize cells by 15 minutes of shaking at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... 150 µl were removed from each well and the remaining 50 µl were mixed with 50 µl of 2X Passive Lysis Buffer (Promega), and incubated at 40 rpm for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... were measured in 10 μL of each supernatant mixed with 10 μL 2X Renilla lysis buffer (RLB) by Renilla Luciferase Assay Kit (Promega) in white 96 well plates on a Berthold plate reader ...
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bioRxiv - Immunology 2022Quote: ... 2X GOTaq colorless master mix (Promega), 0.5 μM of both forward and reverse primers (Table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... 2x GoTaq Green master mix (Promega) was used for DNA amplification by PCR with standard cycling conditions for semi-quantitative PCRs ...
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bioRxiv - Systems Biology 2024Quote: ... Following 2x ProNex purification (Promega NG2001), amplicons were sequenced on an Illumina MiSeq with PE70 read structure ...
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bioRxiv - Cell Biology 2024Quote: ... Tracer 8 (20X) stock solutions were prepared in tracer dilution buffer (Promega, #N291B) and 20% DMSO ...
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bioRxiv - Bioengineering 2024Quote: ... Reporter lysis buffer and luciferin assay solution were purchased from Promega (Madison, WI). Plasmid DNA (pDNA ...
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bioRxiv - Physiology 2021Quote: ... Approximately 3000 animals were collected as a pellet and mixed with the same volume of 2x passive lysis buffer (Promega, E194A) on ice ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then diluted 1:7 in Glo Lysis Buffer (Promega) and incubated 1:1 for 5 minutes in the dark in opaque 96 well plates with NanoGlo Substrate freshly diluted 1:50 in NanoGlo Buffer (Promega) ...
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bioRxiv - Microbiology 2021Quote: ... Samples were lysed in 100μL of Reporter Gene Lysis buffer (Promega) and frozen overnight ...
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bioRxiv - Microbiology 2024Quote: ... Samples were mixed with 5x Green GoTaq® Reaction Buffer (Promega), used as loading dye ...
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bioRxiv - Cell Biology 2024Quote: ... samples were lysed in Passive Lysis Buffer (Promega, Madison, WI, USA) and processed as indicated by the manufacturer ...
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bioRxiv - Zoology 2024Quote: ... Most samples were digested in a mixture of 97% Nuclei Lysis Solution (Promega; from the Wizard Genomic DNA Purification Kit ...
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bioRxiv - Neuroscience 2021Quote: ... All buffers and gradient solutions for nuclei extraction contained 0.01U/ul RNase inhibitor (Promega) and 0.04% BSA.
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bioRxiv - Developmental Biology 2021Quote: ... 2x GoTaq Green Master Mix (M7121, Promega) was used for the PCR reactions ...
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bioRxiv - Biochemistry 2023Quote: ... 5 µL of 2x GoTaq (Promega, #A600A), 0,4 µL forward primer (0,5 µM) ...
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bioRxiv - Neuroscience 2024Quote: ... 2X GO Taq master mix (M7122, Promega), primer pair (0.5 µl each) ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 μl of 2X CellTiterFluor reagent (Promega) was added to the cells ...
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bioRxiv - Microbiology 2021Quote: ... the samples were diluted 1:100 in 1 × passive lysis buffer (Promega) and 5 µl were transferred into a white Nunc 96-well plate ...
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bioRxiv - Neuroscience 2021Quote: ... Heads (10/sample) were homogenized in 20 μL Glo Lysis buffer (Promega), incubated at room temperature for 15 minutes ...
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bioRxiv - Physiology 2020Quote: The protein solution samples were hydrolyzed into peptide segments by trypsin (V9012, Promega (Beijing) Biotech Co. ...
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bioRxiv - Neuroscience 2023Quote: ... eluted in-solution samples were alkylated with N-Ethylmaleimide and digested with trypsin (Promega) using micro S-trap column (ProtiFi) ...