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Citations for GE Life Sciences :
1 - 50 of 1700 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... the beads were boiled with 2 × Laemmli’s sample buffer and subjected to immunoblotting using an Amersham ECL Prime Western Blotting Detection System (GE Healthcare).
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were then boiled in Laemmli sample buffer prepared in house and resolved on a 10 % polyacrylamide gel and transferred to nitrocellulose membranes (GE Healthcare Life Science ...
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bioRxiv - Molecular Biology 2020Quote: Whole cell lysates were extracted from cells in 2x sample buffer and separated on 10% SDS– PAGE gel and transferred to nitrocellulose membranes (GE Healthcare). The membranes were blocked with Odyssey Blocking Buffer in PBS (Licor) ...
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of protein were diluted in Laemmli buffer and separated by SDS-PAGE before transfer onto polyvinylidene difluoride or nitrocellulose membranes (both GE Healthcare Science). After primary antibody incubation (Progranulin (clone 8H10 ...
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bioRxiv - Immunology 2024Quote: ... subjected to 10% SDS-PAGE using Laemmli discontinuous buffer system (59) and transferred into nitrocellulose membranes (GE Healthcare Life science, Buckinghamshire, UK). Staining with Ponceau S was used to verify loading and transfer efficiency ...
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bioRxiv - Molecular Biology 2024Quote: ... Eluent was combined with an additional 2x 20 mL of wash buffer and subsequently applied to a 5 mL HiTrap Q (GE Healthcare) ion-exchange column equilibrated in a buffer containing 25 mM Tris•Cl ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed of unbound probe with a pre-warmed (42°C) 2X saline-sodium citrate (SSC) buffer and detected by phosphor imaging (Storm™ 840, GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... each sample was buffer exchanged a PD-10 desalting column (GE Healthcare) that had been equilibrated with RIPA buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... the sample was concentrated and then buffer exchanged using a Nap10 (GE healthcare) column equilibrated in buffer B (20 mM Tris pH 8.0 ...
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bioRxiv - Plant Biology 2020Quote: ... Suspension was gently load onto two prepared falcon tubes containing equal volumes of 2X isolation buffer and Percoll™ (GE Healthcare-17-0891-02). Samples were centrifuged in a swing bucket centrifuge at 1200Xg for 10 minutes with brakes off ...
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bioRxiv - Bioengineering 2022Quote: ... The eluted protein solution was then buffer exchanged using a PD-10 (GE Healthcare Life Sciences) desalting column.
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bioRxiv - Evolutionary Biology 2022Quote: Solutions of purified rHbs were desalted by passing samples through PD-10 desalting columns (GE Healthcare) that were equilibrated with 0.01 M HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... the samples were submitted to a buffer exchange using HiTrap desalting columns (GE Healthcare). The recombinant proteins were submitted to an additional anion exchange chromatography step ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The sample was formulated using formulation buffer and AKTA flux s (GE Healthcare, USA). After sterile filtration through 0.22-micron PES Sartopore 2 filter (Sartorius Stedim ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluted fraction was neutralized with 2 M Tris pH7.0 and either diluted first 1:1 with 20 mM phosphate pH7.0 buffer (25 mL milk sample) or loaded directly (100 mL milk sample) onto a Q Sepharose FF (GE Healthcare, Uppsala, Sweden) equilibrated with 20 mM phosphate pH7.2 buffer column at 150 cm/h and 90 cm/h ...
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bioRxiv - Biophysics 2022Quote: ... The dialyzed protein solution (∼50 μM) was then loaded into the sample cell in iTC200 (GE Healthcare) after the removal of insoluble materials by centrifugation (10,000×g ...
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bioRxiv - Biophysics 2022Quote: ... The solution was immediately desalted and the buffer exchanged by passing it over a Sephadex G25 (GE Healthcare) XK 50/20 column equilibrated with 20 mM MES-NaOH pH 5.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein solutions were further diluted with buffer B and eIF4A1 fractions subjected to a ResourceQ (GE Healthcare) anion exchange column ...
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bioRxiv - Biochemistry 2020Quote: ... The solution was dialyzed against Buffer C overnight at 4 °C and applied on a Resource Q column (GE Healthcare) that was equilibrated with Buffer C ...
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bioRxiv - Biochemistry 2021Quote: ... and STD samples were mixed and the rehydration solution (7 M urea, 2 M thiourea, 4% CHAPS, 0.5% IPG Buffer 4-7 [GE Healthcare]) was added before overnight IPG-strips passive rehydration (pH gradient 4-7 ...
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bioRxiv - Biophysics 2021Quote: ... aSyn in Tris was buffer exchanged into freshly prepared NaCl and CsI solutions (pH 5.4) before experiments using PD10 dialysis columns (GE Healthcare). Protein concentration was determined from the absorbance measurement at 280 nm on a Nanovue spectrometer using the extinction coefficient for aSyn of 5960 M−1cm−1.
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bioRxiv - Biochemistry 2022Quote: ... 2x TBS) signal was detected with an AI-600 imager (GE Healthcare) using SuperSignal West Dura Extended Duration Substrate (Thermo).
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bioRxiv - Biophysics 2021Quote: ... The sample was then buffer exchanged using a Sephadex G-25 column (GE Healthcare Bio-Sciences, Pittsburgh, PA) to CD buffer (20 mM sodium phosphate ...
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bioRxiv - Biophysics 2020Quote: ... the external buffer of the proteoliposome sample was exchanged using a column of G50 Sephadex (GE Life Sciences) equilibrated in EB ...
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bioRxiv - Cancer Biology 2023Quote: ... SEC-eluted samples were subject to buffer exchange on Fast-Desalt FPLC column HiPrep 26/10 (GE Healthcare) in 50mM Tris-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... The eluted sample was buffer exchanged using size exclusion chromatography in a Superdex 75 26/60 (GE Healthcare) to a myristoylation buffer (50 mM NaH2PO4/Na2HPO4 ...
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bioRxiv - Immunology 2021Quote: ... The protein was eluted with 10 mM maltose solution in the lysis buffer and further purified by size exclusion chromatography using Superose 6 10/300 GL column (GE Healthcare) equilibrated with 30 mM HEPES at pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... Erythrocyte lysis was performed using ammonium-chloride-potassium (ACK) buffer and leukocytes were isolated by gradient centrifugation using Percoll solution (GE Healthcare). Lung tissue leukocytes were filtered again and antibody staining for flow cytometry was performed.
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bioRxiv - Immunology 2020Quote: ... An equal volume of buffer (2 mM EDTA in PBS) and blood was mixed and carefully layered over 5ml Ficoll Hypaque solution (GE Healthcare). After centrifugation for 20 min at 900 g (with no break ...
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bioRxiv - Immunology 2020Quote: ... the surface was saturated with two sequential injections of a 20 μM knob domain solution in HBS-EP buffer (GE healthcare), using a flow rate of 30 μL/min and contact time of 300 seconds ...
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bioRxiv - Bioengineering 2023Quote: ... PBMCs were isolated from peripheral blood samples of a healthy volunteer by density gradient centrifugation using Ficoll-Paque solution (GE Healthcare) and were freshly transferred to NSG mice ...
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bioRxiv - Immunology 2024Quote: ... Samples were centrifuged at 220xg for 10 min at 4°C and then resuspended in a 22% Percoll solution (GE Healthcare) with a PBS layer floated on top ...
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bioRxiv - Microbiology 2020Quote: ... Protein lysates were obtained by lysing infected cell monolayers in 4x Laemmli sample buffer and were analysed by semi-dry Western blotting onto Hybond 0.2µM polyvinylidene difluoride (PVDF) membrane (GE Healthcare). Membranes were incubated with rabbit antisera diluted in PBS with 0.05% Tween-20 containing 5% dry milk (Campina) ...
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bioRxiv - Biophysics 2021Quote: Protein samples were exchanged into a H-buffer using a PD-10 G-25 media desalting column (GE Healthcare) to induce droplet formation ...
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bioRxiv - Molecular Biology 2020Quote: ... the buffer of the DNA samples was exchanged to SDAD cross-linking buffer (100 mM NaHCO3 pH 8.3) using MicroSpin S400 columns (GE Healthcare). 1 μg of dsDNA was incubated with 2 mM SDAD cross-linker in 25 μl of SDAD cross-linking buffer at 25 °C overnight ...
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bioRxiv - Biochemistry 2020Quote: The sample was desalted into Buffer A using a HiPrep 53 ml 26/10 desalting column (GE Healthcare, Chicago, IL) with 14 ml injections at 9 ml/min for all steps ...
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bioRxiv - Biophysics 2022Quote: ... The sample was exchanged into the D2O buffer using a NAP-5 Sephadex G-25 column (GE Healthcare, Chicago, IL). For dUMP and TMP (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... the protein sample was concentrated and exchanged with phosphate buffer saline (PBS, pH 7.5) using a PD10 column (GE Healthcare) and stored at -80°C ...
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bioRxiv - Microbiology 2022Quote: ... the sample was concentrated and gel-filtered in the same buffer on a HiLoad 16/60 Superdex 200 (GE Healthcare) column ...
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bioRxiv - Biochemistry 2022Quote: ... The precipitated pellet was resuspended in Buffer A and the solution subjected to Ni(II) immobilized affinity chromatography using a 5 mL HisTrap FF column (GE Healthcare Life Sciences) with a gradient from 100% buffer A to 100% buffer B (25 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: The AR9 nvRNAP core sample was polished and buffer-exchanged using size exclusion chromatography on a Superdex 200 10/300 (GE Healthcare) column equilibrated with buffer D containing 100 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... The reaction was quenched in sample loading buffer at different timepoints and visualized by scanning the SDS-PAGE in the Typhoon Imager (GE Healthcare).
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bioRxiv - Biophysics 2023Quote: ... The successfully cleaved samples were subsequently buffer exchanged using a Superdex S200 5/150 GL or 10/300 increase column (GE Healthcare) connected to an Agilent HPLC 1260 Affinity equipped with a fraction collector ...
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bioRxiv - Biochemistry 2022Quote: ... Removal of free dyes outside liposomes and exchange of outside buffer was achieved by passing samples through a desalting column (PD-10, GE Healthcare) equilibrated with the outside buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Bound proteins were eluted by boiling in SDS–PAGE sample loading buffer and analyzed by western blot using an anti-GST antibody (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... iodoacetamide solution (GE Healthcare) was added ...
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bioRxiv - Immunology 2022Quote: ... iodoacetamide solution (GE Healthcare) was added to a final concentration of 40 mM and the reaction was allowed to proceed at room temperature in the dark for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... ECL solution (GE Healthcare) was used to develop a chemiluminescent signal in an X-ray film (GE Healthcare).
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bioRxiv - Zoology 2020Quote: ... Proteins were separated in the second dimension on a 14% SDS polyacrylamide gel (Laemmli, 1970) in a Hoefer600 cell (GE Healthcare) for 15 min at 15 mA (100 V/15 W limits ...
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bioRxiv - Cell Biology 2022Quote: ... 50 μg of protein per sample were solved in urea lysis buffer and labeled with 250 pmol CyDye DIGE Fluor dyes (GE Healthcare, Germany). Subsequently ...