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Citations for R&D Systems :
1 - 50 of 328 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... Following two washes, the samples were incubated in 200 μl of substrate solution (tetramethylbenzidine, TMB solution) (R&D systems) per sample for 20 min ...
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bioRxiv - Bioengineering 2021Quote: ... Fixable Yellow samples were subsequently fixed using Flow Cytometry Fixation Buffer (R&D Systems), centrifuged ...
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bioRxiv - Cancer Biology 2023Quote: ... The samples were then incubated with 1X Fixation/Permeabilization Buffer (R&D systems, Cat. No. FC007) for 12 minutes at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were then incubated with 1X Fixation/Permeabilization Buffer (R&D systems, Cat. No. FC007) for 12 minutes at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... all cells were spun down to remove the antibody solution and resuspended in fresh flow cytometry staining buffer (R&D Systems #FC001). Cells were then passed through round bottom Falcon tubes for flow cytometric analysis using a BD LSR Fortessa.
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed 3 times with wash buffer and 100 ul of samples and standards were added (R&D Systems, UK). Plates were left to incubate at room temperature for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... stimulation medium (2X concentration) was prepared by adding 400 ng/mL HA-tagged rhCD40L (R&D Systems) and 100 ng/mL anti-HA tag antibody (clone 543851 ...
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bioRxiv - Bioengineering 2020Quote: ... After the STOP solution (R&D system), the plates were read at multiple wavelengths (the optical density at 550 nm wavelength subtracted from the density at 450 nm ...
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bioRxiv - Bioengineering 2020Quote: ... After the STOP solution (R&D system), the plates were read at multiple wavelengths (the optical density at 550 nm wavelength subtracted from the density at 450 nm ...
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bioRxiv - Immunology 2022Quote: ... After the STOP solution (R&D system), the plates were read at multiple wavelengths (450 nm and 550 nm ...
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bioRxiv - Immunology 2020Quote: ... developed using TMB solution (R&D Systems, #DY999) and read at 450nm.
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bioRxiv - Cell Biology 2022Quote: ... 15 μL of 2x concentrated ubiquitin-rhodamine 110 (Ub-Rho110; 200 nM, FAC 100 nM [R&D Systems, U-555-050]) in USP30 activity assay buffer was dispensed into the compound-rhUSP30 containing plate using the Dragonfly Discovery liquid dispenser and fluorescence read on the FLIPR TETRA plate reader (Molecular Devices) ...
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bioRxiv - Bioengineering 2022Quote: ... The streptavidin modified nanovial solution was then mixed with a solution containing an antibody cocktail comprising 8 µg/mL anti-mouse CD45 (R&D Systems) biotinylated according to manufacturer instructions ...
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bioRxiv - Bioengineering 2022Quote: ... and resuspended in 400 μL buffer (Flow Cytometry 1x Staining Buffer, R&D Systems, Minneapolis, MN). The 400 μL suspension for each experimental condition was then distributed into four 100 μL aliquots in 1.5 mL conical tubes representing the unstained control ...
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bioRxiv - Cancer Biology 2020Quote: ... samples first underwent activation using the Sample Activation Kit 1 (R&D Systems, Minneapolis, MN) before sample incubation ...
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bioRxiv - Bioengineering 2020Quote: ... 50 μl stop solution (2NH2SO4, DY994, R&D systems) was added ...
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bioRxiv - Cancer Biology 2021Quote: ... 1x Energy regeneration solution (#B-10 R&D systems), 75 mM ubiquitin (#U-100H R&D systems ...
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bioRxiv - Bioengineering 2020Quote: ... 100 μl of stop solution (Sulfuric acid) (R&D systems) was added ...
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bioRxiv - Neuroscience 2022Quote: ... Pre warmed sulfuric acid stop solution (DY994, R&D Systems) at 37 °C was used to stop the reaction and the absorbance was monitored at 450 nm with a Fluorostar galaxy plate reader.
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bioRxiv - Neuroscience 2022Quote: ... Pre-warmed sulfuric acid stop solution (DY994, R&D Systems) at 37 °C was used to stop the reaction and absorbance at 450 nm was measured in a Fluorostar galaxy plate reader.
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bioRxiv - Microbiology 2022Quote: ... The assay was developed with substrate solution (R&D systems) and the reaction was stopped with 1M H2SO4 and analyzed with a Tecan Sunrise microplate reader at (Tecan ...
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bioRxiv - Neuroscience 2024Quote: ... and 2.5% trypsin-EDTA solution (R&D systems, Cat. B81710) were added to the tubes and incubated for 30 min at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... by incubation in Cultrex Organoid Harvesting Solution (R&D Systems) for 1 hour on ice and mechanical dissociation with a pipette ...
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bioRxiv - Cell Biology 2022Quote: ... A stock solution of recombinant mouse Wnt3A (R&D systems) was prepared at 40 μg/ml in 0.1% BSA/PBS and used between 10-100 ng/ml concentration in culture.
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bioRxiv - Immunology 2024Quote: Organoids were isolated using organoid harvesting solution (R&D Systems). Dissolved CultrexTM BME was aspirated ...
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bioRxiv - Molecular Biology 2024Quote: ... solidified and immersed in prechilled lysis solution (R&D systems) overnight at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... PBS was removed and 50 μl of cell lysis buffer (Cell Lysis Buffer 2, R&D Systems, # 895347) were added ...
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bioRxiv - Bioengineering 2021Quote: ... washed with Permeabilization Buffer (R&D Systems), centrifuged ...
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bioRxiv - Microbiology 2020Quote: ... 50 µL of each solution A and B (R&D Systems) was added into each well ...
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bioRxiv - Neuroscience 2022Quote: ... 100µL of collagen type I solution (Corning or R&D systems, 3 mg/mL with a pH adjusted to 7.0-7.2 with NaOH ...
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bioRxiv - Bioengineering 2022Quote: ... and developed for 10 min using substrate solution (R&D systems). The reaction was stopped with 2N H2SO4 and plates were measured using a microplate reader (Tecan Sunrise ...
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bioRxiv - Biochemistry 2023Quote: ... Each well was loaded with 30 µL (50 µg protein) lysate with 70 µL of Buffer A buffer containing 20μM Suc-LLVY-AMC (R&D Systems). Assay parameters ...
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bioRxiv - Biochemistry 2021Quote: Four 4× stock solutions were prepared containing (i) Ube1 (R&D Systems) + UbcH5c/UBE2D3 (R&D Systems ...
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bioRxiv - Immunology 2024Quote: ... or of Cultrex Organoid Digestion solution (R&D systems, #3700-100-01). Chips were incubated for 45 minutes at 4°C to allow diffusion of the digestion solution from the upper to the lower channel ...
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bioRxiv - Synthetic Biology 2024Quote: ... 10% BSA ELISA reagent diluent/blocking solution concentrate (DY995, R&D systems) and TMB substrate (421501 ...
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bioRxiv - Systems Biology 2024Quote: ... cells were stained with 6 µM Calcein AM solution (R&D Systems) for 15 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were lysed overnight in Comet Assay Lysis Solution (R&D Systems). The next day ...
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bioRxiv - Neuroscience 2020Quote: ... and 10mM citrate buffer pH 6.0 at 95°C x 5 mins for Prox1 (neutral buffer CTS016, R&D Systems, Minneapolis, MN). Sections were then blocked with 10% or 5% (for Tbr2 ...
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bioRxiv - Cancer Biology 2020Quote: ... diluted in DAB chromogen buffer (860005, R&D Systems). Positive and negative controls are described below.
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bioRxiv - Biochemistry 2021Quote: Acvr1[R206H]/+and Acvr2a −/−;Acvr2b −/−;Bmpr2 −/−;Acvr1[R206H]/+ mES cells were dissociated using non-enzymatic cell dissociation buffer (MiliPore, S-004-B) and resuspended in the flow cytometry staining buffer (R&D systems, FC001). After 15 minutes of blocking (Thermofisher ...
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bioRxiv - Developmental Biology 2023Quote: pH 6.0 Tris-EDTA buffer was used as the antigen-retrieval buffer for the following antibodies: CD31 (R&D systems; AF3628; 1:500), Aggrecan (Millipore ...
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bioRxiv - Developmental Biology 2023Quote: pH 9.0 Tris-EDTA buffer was used as the antigen-retrieval buffer for the following antibodies: CD31 (R&D systems; AF3628; 1:500), SOX9 (Millipore ...
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bioRxiv - Immunology 2023Quote: ... Total TGF-β levels were determined by acid activation of the latent TGF-β1 in the sample using the sample activation kit 1 (DY010) (R&D Systems).
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bioRxiv - Bioengineering 2021Quote: ... Cerebrum primary antibody solution contained mouse anti-GFAP IgG (R&D Systems, MAB2594) and DAPI to visualize astrocytes and cell nucleus respectively ...
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bioRxiv - Cancer Biology 2022Quote: ... 3-day old organoids were harvested with Cultrex Harvesting Solution (R&D Systems) and frozen in Recovery Cell Culture Freezing Medium (GIBCO) ...
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bioRxiv - Cancer Biology 2022Quote: Early passage organoids were collected using Cultrex Organoid Harvesting Solution (R&D systems), washed with ice-cold PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... 3-day old organoids were harvested with Cultrex Harvesting Solution (R&D Systems) and frozen in Recovery Cell Culture Freezing Medium (GIBCO) ...
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bioRxiv - Neuroscience 2022Quote: ... After three washes with washing buffer (WA126, R&D systems), 100 µL of a solution containing 0.001 mg/mL of the detection biotinylated polyclonal antibody (BAF1559 ...
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bioRxiv - Neuroscience 2022Quote: ... washed 3× in 1× wash buffer (895003, R&D Systems), blocked in 1% BSA for 1 hour at room temperature (RT) ...
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bioRxiv - Neuroscience 2020Quote: ... the slides were immersed in lysis solution (Cat# 4250-050-01, R&D SYSTEMS) overnight at 4 °C in the dark ...