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Citations for abcam :
1 - 50 of 1620 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... This was followed by addition of Laemmli buffer to 1x and blotting with H3 (Abcam, ab1791), CHTOP ...
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bioRxiv - Bioengineering 2023Quote: ... MBV samples were incubated in vanadium solution (Abcam) for 15 min at room temperature for negative staining ...
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bioRxiv - Bioengineering 2024Quote: EV samples were incubated in vanadium solution (Abcam) for 15 min at room temperature for negative staining ...
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bioRxiv - Molecular Biology 2023Quote: Purified brain mitochondria were solubilized in Laemmli buffer and processed for Western blotting using standard procedures and Total OXPHOS Antibody Cocktail (#ab110411, Abcam).
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bioRxiv - Molecular Biology 2024Quote: ... The slides were rinsed in PBS and blocked in 200µl blocking buffer (2X SSC and 1% BSA) and then incubated with an AF647 Anti-Brd4 antibody (Abcam) diluted at 1:100 in antibody diluent (2X SSC and 1% BSA ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction was stopped by addition of equal volumes of 2X non-reducing SDS-PAGE loading buffer (Abcam #ab139472). Reactions were analyzed by Coomassie staining and western blotting with anti-ubiquitin antibody (Merck ...
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bioRxiv - Cancer Biology 2020Quote: ... Beads were resuspended in 50uL of 1x Laemmli buffer and visualized by SDS-PAGE and Western Blot (α-Twist 2C1A, α-Snail L70G2, α-Sox9 Abcam ab3679).
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bioRxiv - Neuroscience 2023Quote: ... sections were rinsed in blocking buffer (2x 10 min) and incubated with secondary antibodies: Alexa Fluor 488 goat anti-mouse (1:1000, Abcam), Alexa Fluor 594 goat anti-rabbit (1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... samples were immersed in 0.4x diluted Mayer’s Modified Hematoxylin solution(Abcam, #64795) for 1 min and rinsed again in water for 3 min ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed in 2x SSC (3 x 5 min) and incubated in blocking solution containing secondary antibody (anti-chicken Alexa 488, Abcam, 1:1500) for 2 hours at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... samples were incubated in citric acid buffer (10X citric acid buffer pH 6.0, ab64214, Abcam, Cambridge, UK) at 100°C for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated in 2x PBS with 0.2% Tween and blocking buffer (1:1) with primary antibodies rabbit anti-AUF1 (Abcam, 1:5000), rabbit anti-phospho-AUF1 (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were pulverized on ice in cell lysis buffer (Abcam, ab152163) with protease inhibitor cocktail (ThermoFisher ...
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bioRxiv - Pathology 2021Quote: ... Incubation of the samples in a 5% silver nitrate solution (Abcam® ab150687) took place for 45 minutes with 100 Watt incandescent light exposure ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were then stained with Picro-sirius red solution (Cat. # ab150681, Abcam, UK) for 1 hour ...
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Cell-Type Specific Profiling of Histone Post-Translational Modifications in the Adult Mouse StriatumbioRxiv - Neuroscience 2022Quote: ... Nuclei from one striatum (~10,000) were washed 2X and then resuspended in Digitonin Buffer with either anti-H3K4me3 (Antibody 1: Abcam, Ab8580 (Figure 2); Antibody 2 ...
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bioRxiv - Neuroscience 2024Quote: The collected organoids and cell samples were treated with RIPA buffer (Abcam) containing 1X protease inhibitor cocktail (MCE) ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were incubated in primary antibody solution (Rat anti-CTIP2, 1:500, Abcam, ab18465) overnight in 0.2% Triton x-100 and 2% NGS in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were homogenized in 100 µL of Assay Buffer II/Cholesterol Assay Buffer (Abcam, Cat # ab65390, Cambridge, MA, USA) using a Dounce homogenizer with 12 passes ...
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bioRxiv - Immunology 2021Quote: ... stained with 1:200 diluted secondary antibodies solution in blocking buffer (ab150063 Abcam, ab150061, Abcam) for 4 hours at room temperature ...
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bioRxiv - Immunology 2021Quote: ... stained with 1:200 diluted secondary antibodies solution in blocking buffer (ab150063 Abcam, ab150061, Abcam) for 4 hours at room temperature ...
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bioRxiv - Immunology 2023Quote: ... stained with 1:200 diluted secondary antibodies solution in blocking buffer (ab150063 Abcam, ab150061, Abcam) for 4 hours at room temperature ...
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bioRxiv - Immunology 2023Quote: ... stained with 1:200 diluted secondary antibodies solution in blocking buffer (ab150063 Abcam, ab150061, Abcam) for 4 hours at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were counterstained incubating for 30 seconds in a Mayer’s Modified Haematoxylin solution (Abcam, #220365).
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bioRxiv - Genomics 2021Quote: ... loading OD-normalized cells in sample buffer and using rabbit anti-GFP (Abcam) and mouse anti-PGK1 (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were resuspended again in FACS buffer containing DRAQ7 (Abcam, cat. #ab109202) at a 1:100 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated with primary antibody buffer solution with 1:200 rabbit anti-NeuN (ab177487, Abcam), goat anti-Aquaporin IV (AB2216 ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were incubated in PHT solution containing goat anti-mouse Alexa 647 (1:500, Abcam). The samples were again washed with 1x PBS containing 0.3% Triton X100 and mounted in Vectashield mounting medium (Vector Laboratories) ...
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bioRxiv - Cancer Biology 2020Quote: ... The fixed samples were blocked in detergent-free blocking solution [1% normal donkey serum (ab7475, abcam) and 3% BSA in PBS] without membrane permeabilization by detergents ...
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bioRxiv - Cancer Biology 2020Quote: ... coverslips were blocked for one hour at room temperature in blocking solution (Aqua block buffer, Abcam ab166952) and then transferred to a carrier solution (Aqua block ...
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bioRxiv - Bioengineering 2024Quote: ... samples were incubated with blocking solution (1× PBS containing 10% normal goat serum (NGS, Abcam, Cambridge, UK), 1% bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 µL of each sample was lysed in 1 x RIPA lysis buffer (ab156034, Abcam) supplemented with 1 x Halt™ Protease Inhibitor Cocktail (Thermo Fisher ...
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bioRxiv - Developmental Biology 2021Quote: ... slides were first deparaffinized and antigen retrieval was performed using either citrate buffer or HIER solution (Abcam, ab208572). Successive steps were followed as previously described22 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated with a solution of D-Wash buffer and Rabbit-antimouse IgG (1:200, Abcam, ab6709), followed by a wash step ...
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bioRxiv - Pathology 2021Quote: ... The samples were then washed and incubated for 3 minutes in a sodium thiosulphate solution (Abcam® ab150687). Samples then were rinsed with water and incubated in nuclear fast red solution (Sigma-Aldrich N8002 ...
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bioRxiv - Bioengineering 2020Quote: ... 300 μL of working solution was added to each sample (1 μL Phalloidin-iFluor 488 Reagent (Abcam, ab176753) per 1 mL 1% BSA in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... All samples were stained with 1 mg/mL 4′,6-diamidino-2-phenylindole (DAPI Staining Solution ab228549, Abcam). Cav-1 (ab2910 ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue samples were incubated overnight in a solution containing both rabbit anti-hASYN (1:10000; Abcam, RRID:AB_2537217, MJFR1) and mouse anti-SynO2 (1:1000 ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were then washed six times with PMEG buffer and incubated with anti-ATG8a (Abcam, ab77003) and R13-ATG8a antibodies in 1x phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed in wash buffer (PBS with 0.05% Tween) and incubated in blocking solution with rabbit anti-sheep IgG (Abcam) at 1:40,000 dilution for 1 hour at room temperature and visualised on LiCor Odyssey Fc (Invitrogen).
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bioRxiv - Neuroscience 2023Quote: ... Deparaffinized and rehydrated slides were submerged in 1X antigen retrieval solution (100X Tris-EDTA Buffer, pH 9.0, ab93684, Abcam) and placed in a tissue steamer (IHC-Tek Epitope Retrieval Steamer Set ...
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bioRxiv - Genomics 2024Quote: ... cells were incubated with a solution of D-Wash buffer and Rabbit-anti-mouse IgG (1:200, Abcam, ab6709), followed by a wash step ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated overnight with the primary antibody solution at 4 °C (blocking buffer solution contained primary antibodies with the following dilution: ch α GFP 1:2000, Abcam; rb α βIV-spectrin 1:1000, self-made 4). Subsequently ...
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bioRxiv - Neuroscience 2021Quote: 2x treatments described in the previous section had Draq7 (abcam ab109202) added at 1:150 ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were blocked at RT for 30 min in blocking solution (1x PBS3T 5% normal goat serum (Abcam ab138478)) and incubated in primary antibody dilution buffer (antibody diluted in 1x PBS3T and 1% BSA ...
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bioRxiv - Immunology 2022Quote: ... we fluorescently labeled ∼300,000 CAR T cells at 37°C for 30 minutes in 1:1 (v/v) solution of live cell staining buffer (Abcam) and RPMI-1640 (Corning ...
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bioRxiv - Bioengineering 2024Quote: ... Primary antibody solution for Aldh1l1 and vimentin staining: 2 mL blocking buffer containing 20 μL anti-Aldh1l1 antibody (Abcam, AB56777) and 20 μL anti-vimentin (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies were first validated by immunohistochemistry and immunofluorescence staining of FFPE tissue samples using a single antigen retrieval method consisting of heat mediated antigen retrieval using the universal antigen retrieval solution (Abcam). Based on the relative immunofluorescence signal antibodies were ranked and then pair with the appropriate metal for conjugation to maximise signal to noise ratio ...
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bioRxiv - Cancer Biology 2021Quote: ... heat-induced epitope retrieval was performed by boiling the samples in a steamer for 30 minutes in antigen unmasking solution (Abcam, antigen retrieval buffer ...
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bioRxiv - Microbiology 2020Quote: ... which are the ratio of sample serum absorbance to the absorbance of a manufacturer-provided solution containing the upper limit of the reference range of non-infected biological sample (“cutoff” (Abcam) or “calibrator” (Euroimmun)) ...