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No products found
because this supplier's products are not listed.
Fahmi Mesmar, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
PESTANAL® analytical grade tefluthrin (2,3,5,6-tetrafluoro-4-methylbenzyl 3-[(1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl]-2,2-dimethylcyclopropanecarboxylic acid) was purchased from Sigma Aldrich. Tefluthrin stock solutions were prepared in dimethyl sulfoxide (DMSO ...
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No products found
because this supplier's products are not listed.
Qinjian Li, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 2-[6-(4’-hydroxy) phenoxy-3H-xanthen-3-on-9-yl] benzoate (HPF) from Molecular Probes® and Horse radish peroxidase (HRP ...
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No products found
because this supplier's products are not listed.
Fabian M. Meyer, et al.,
bioRxiv - Microbiology 2023
Quote:
... cells were resuspended in 25 µl PBS together with 0.25 µl of 5 mM 3-[[(7-Hydroxy-2-oxo-2H-1-benzopyran-3-yl) carbonyl]amino]-D-alanine hydrocholoride (HADA) (Tocris) dissolved in DMSO ...
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No products found
because this supplier's products are not listed.
Krzysztof Mikołajczyk,
bioRxiv - Biochemistry 2024
Quote:
... and untransfected cells were cultured for 2 weeks in a complete medium containing 3 μM N-[(1R,2R)-1- (2,3-dihydro-1,4-benzodioxin-6-yl)-1-hydroxy-3-pyrrolidin-1-ylpropan-2-yl]nonanamide (Genz-123346) (Merck, Darmstadt, Germany), a glucosylceramide synthase inhibitor [26].
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No products found
because this supplier's products are not listed.
Johnson Ung, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 20 μL of [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] (MTS) (Promega, #G3582) were added and incubated at 37°C for 3 hours ...
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No products found
because this supplier's products are not listed.
Zhe Chen, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... stained en bloc with 1% uranyl acetate (Electron Microscopy Sciences) overnight at 4°C ...
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No products found
because this supplier's products are not listed.
Annamarie E. Allen, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Cells were incubated in treatment media for the indicated period of time and then MTS reagent ((3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) purchased from Abcam, (ab197010 ...
-
No products found
because this supplier's products are not listed.
Priyanka Yolmo, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... IL-21 (20 ng/ml, Cat# 210-21, Peprotech, NJ, USA) and TLR7 agonist – Imiquimod (R837 ...
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No products found
because this supplier's products are not listed.
Lorenzo Calviello, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... and the NIR fluorescent adaptor (5′-OH-AGATCGGAAGAGCGGTTCAGAAAAAAAAAAAA/iAzid eN/AAAAAAAAAAAA/3Bio/-3′) was ligated to the RNA using truncated RNA ligase 2 K227Q (NEB) overnight at 16°C ...
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No products found
because this supplier's products are not listed.
Maryam Rahmati Ishka, et al.,
bioRxiv - Plant Biology 2024
Quote:
... 20-hydroxy arachidic acid (20:0-ωOH) (Cayman Chemical Company, Ann Arbor, Michigan) standards were input at 2.5 μg ...
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No products found
because this supplier's products are not listed.
Carlos J. Garcia, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Authentic standards of 3,7-Dihydroxy-5-cholan-24-oic Acid (chenodeoxycholic acid) and 3-Hydroxy-11-oxo-5-cholan-24-oic Acid (3-oxo-chenodeoxycholic acid) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). The N-(3α,7α,12α-trihydroxy-5β-cholan-24-oyl)-glycine (glycocholic acid) ...
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No products found
because this supplier's products are not listed.
Blanca Salazar-Sarasua, et al.,
bioRxiv - Plant Biology 2021
Quote:
... in paraffin-embedded sections (8µm) and color was detected with 5-bromo-4-chloroindol-3-yl phosphate/nitrateblue tetrazolium (BCIP/NBT) (Roche).
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No products found
because this supplier's products are not listed.
Gina Partipilo, et al.,
bioRxiv - Bioengineering 2024
Quote:
... disodium;4-[3-pyridin-2-yl-6-(4- sulfonatophenyl)-1,2,4-triazin-5-yl]benzenesulfonate hydrate (Ferrozine, VWR) All media components were autoclaved or sterilized using 0.2 μm PES filters.
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No products found
because this supplier's products are not listed.
Meghan E. Wynne, et al.,
bioRxiv - Cell Biology 2022
Quote:
... washed 3 times with ice cold PBS (Corning, 21-040), and then incubated with PBS with 10mM EDTA for 30 minutes at 4°C ...
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No products found
because this supplier's products are not listed.
Felix Lange, et al.,
bioRxiv - Cell Biology 2024
Quote:
... The following antibodies were used: anti-GFP (JL-8; 1:20, Clontech, Saint-Germain-en-Laye, France), anti-PHB1 (EP2803Y ...
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No products found
because this supplier's products are not listed.
Eva Hitz, et al.,
bioRxiv - Microbiology 2021
Quote:
... Protein lysates were separated on NuPage 5-12% Bis-Tris or 3-8% Tris-Acetate gels (Novex, Qiagen) using MES running buffer (Novex ...
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No products found
because this supplier's products are not listed.
Fabien Thery, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 3-8% Criterion XT tris- acetate gel (#3450130, Biorad) or 4-15% Criterion TGX gel (#5671083 ...
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No products found
because this supplier's products are not listed.
Yasuhiro Oda, et al.,
bioRxiv - Microbiology 2024
Quote:
... 20 mM acetate and 0.3% Difco Agar Noble (BD) that were held in a 60°C water bath ...
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No products found
because this supplier's products are not listed.
Shin Murakami, et al.,
bioRxiv - Microbiology 2022
Quote:
... The target sequence for the ACE2 gene (5′-TGCTGCTCAGTCCACCATTG-3′) was designed using CRISPR direct (https://crispr.dbcls.jp) and cloned into plentiCRISPR plasmids (21) (Addgene plasmid #52961 ...
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No products found
because this supplier's products are not listed.
Adriaan van der Graaf, et al.,
bioRxiv - Genetics 2020
Quote:
... or IL-21 (3 ng/ml, Biolegend, cat 571204) (n=8 samples per condition ...
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No products found
because this supplier's products are not listed.
Carsten Frühbeis, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 20 ml/l NeuroBrew-21 (Miltenyi Biotec), 2 mM L-glutamine ...
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No products found
because this supplier's products are not listed.
Senthil T. Kumar, et al.,
bioRxiv - Neuroscience 2020
Quote:
... at 10-20 μg/mL concentration in 10 mM acetate solution (GE Healthcare) at pH 4.5 to reach a final surface ligand density of around 2000-4000 response units (RUs) ...
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No products found
because this supplier's products are not listed.
Stefan Bresson, et al.,
bioRxiv - Cell Biology 2020
Quote:
... blocked with 5% skimmed milk and probed successively with anti-Flag (Agilent, 200474-21) and anti-Rat (Licor ...
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No products found
because this supplier's products are not listed.
Felix Scharte, Rico Franzkoch, Michael Hensel,
bioRxiv - Microbiology 2022
Quote:
... Grids were post-stained for 30 min with 2% uranyl acetate (Roth) and 20 min with 3% lead citrate (Leica). TEM images were acquired using a Zeiss Leo 912 Omega (Zeiss ...
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No products found
because this supplier's products are not listed.
Mehdi Baratchian, et al.,
bioRxiv - Physiology 2020
Quote:
... AR (EMD Millipore: PG-21 and Santa Cruz Biotechnology: N-20), PSA (Cell signaling ...
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No products found
because this supplier's products are not listed.
Marco S. Kaiser, et al.,
bioRxiv - Physiology 2021
Quote:
... 3’ and 5’ adaptors (Illumina) were ligated and the resulting product was reverse transcribed to generate cDNA by PCR ...
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No products found
because this supplier's products are not listed.
Michelle E. Pizzo, et al.,
bioRxiv - Neuroscience 2024
Quote:
... NK cells were stimulated overnight with IL-21 (20 ng/mL, R&D Systems, Minneapolis, MN). Effector cells were added to target cells at 25:1 effector:target cells ratio (250,000 cells/well ...
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No products found
because this supplier's products are not listed.
Hae Young Ko, et al.,
bioRxiv - Cancer Biology 2022
Quote:
Mouse orthotopic xenograft models (4-5 mice per each group) were intravenously injected with 14C-acetate (3 μCi, PerkinElmer) in 200 μL saline and perfused with 4% paraformaldehyde (PFA ...
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No products found
because this supplier's products are not listed.
Syed-Rehan A. Hussain, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... The selected sgRNA with additional 20 bp RP-loop [5’TCTCCCTGAGCTTCAGGGAG-3’] at the 5’ end of guide RNA was custom synthesized by Genscript, cloned into plasmid pUC57 with unique restriction sites (Pcil ...
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No products found
because this supplier's products are not listed.
Tarek E. Moustafa, et al.,
bioRxiv - Bioengineering 2024
Quote:
20 µm Polystyrene beads (18329-5, Polysciences, USA) were embedded in NOA73 (NOA73 ...
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No products found
because this supplier's products are not listed.
Alexandria Ragsdale, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
Total RNA from six whole 20-21 dpf offspring (3 control offspring, 3 treatment offspring) was extracted using a Zymo Duet extraction kit (Zymo, NZ). The integrity of RNA samples was determined using an Agilent RNA 6000 Nano chip on an Agilent 2100 Bioanalyzer to check that the samples had an RNA Integrity Number (RIN ...
-
No products found
because this supplier's products are not listed.
Hortense Decool, et al.,
bioRxiv - Microbiology 2021
Quote:
BHK-21 cells (clone BSRT7/5) constitutively expressing the T7 RNA polymerase (21) were grown in Dulbeco Modified Essential Medium (Lonza) supplemented with 10% fetal calf serum (FCS) ...
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No products found
because this supplier's products are not listed.
Yoshie Endo, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 21] with 1/100 IB4-DyLight649 (DL-1208-5, Vector Laboratories). Then ...
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No products found
because this supplier's products are not listed.
Liam K. R. Sharkey, et al.,
bioRxiv - Microbiology 2023
Quote:
... The proteins were spin concentrated to 5 mg / ml (3 kDa MWCO, vivaspin 20, Sartorius), aliquoted ...
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No products found
because this supplier's products are not listed.
Harald Schuhwerk, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... IHC or IF staining as described previously 21 (cl. Casp. 3: Cell Signaling Technology, 9661S ...
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No products found
because this supplier's products are not listed.
Miranda L. Curtiss, et al.,
bioRxiv - Immunology 2022
Quote:
50μg biotinylated NS1 protein or 50μg (4-hydroxy-3-nitrophenyl)-acetyl(15)-OVA (NP-OVA) was adsorbed to 100μg alhydrogel alum (InVivoGen) in a total of 200μL per mouse for 30 min at room temperature ...
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No products found
because this supplier's products are not listed.
Charles-Francois V. Latchoumane, et al.,
bioRxiv - Bioengineering 2020
Quote:
Sprague-Dawley rats were obtained for the 20 weeks (n = 33, Harlan Laboratories, 7 weeks old; males only) and the 10 weeks experiments (n = 21, Charles River Laboratories, 3 weeks old; males only), and randomly assigned to each treatment ...
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No products found
because this supplier's products are not listed.
Thomas Parmentier, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and Neurotrophin-3 (NT-3; 20 ng/ml, Stemcell technologies) and the culture medium was changed every 3-4 days ...
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No products found
because this supplier's products are not listed.
John K. Mich, et al.,
bioRxiv - Neuroscience 2023
Quote:
... the following primers were used: (5’-GGTTTCCCGCAGAACCTGAA-3’) and (5’-CCATCGCTCGACCAGTTTAGT-3’) (Jackson Laboratories)
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No products found
because this supplier's products are not listed.
Yili Zhang, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Intracellular recordings from MNs were made with 10-20 MΩ sharp electrodes filled with 3 M potassium acetate connected to an Axoclamp 2-B amplifier (Molecular Devices). Data acquisition and analyses of resting potential ...
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No products found
because this supplier's products are not listed.
Nicholas B. Chamberlain, Ted Hackstadt,
bioRxiv - Microbiology 2022
Quote:
... AM readings were performed by measuring fluorescence at 340/11 nm and 380/20 nm excitation and 508/20 emission every 20 seconds for 5 minutes using a Cytation 5 (BioTek). The solution was replaced with Ca2+-free Ringer’s solution containing either 2 μM thapsigargin (TG ...
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No products found
because this supplier's products are not listed.
Naofumi Amioka, et al.,
bioRxiv - Pathology 2024
Quote:
... and en face images were captured by a digital camera (DS-Ri1; Nikon). Atherosclerotic lesions were traced manually on the images from the ascending aorta to the descending thoracic aorta that was 1 mm distal from the left subclavian artery using a Nikon NIS-Elements software (NIS-Elements AR 5.11.0.) ...
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No products found
because this supplier's products are not listed.
Sushant Suresh, et al.,
bioRxiv - Biochemistry 2024
Quote:
... and the solution was centrifuged for 45 min at 20,000g at 1 °C (Beckman Coulter J2-21, JA-20 rotor). The supernatant was then loaded onto a 5 ml HisTrap column (Cytiva ...
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No products found
because this supplier's products are not listed.
Johanna Tüshaus, et al.,
bioRxiv - Neuroscience 2020
Quote:
... conditioned media was collected and filtered through Spin-X 0.45 µM cellulose acetate centrifuge tube filter (#8163, Costar) and stored at −20°C in protein Lobind tubes (Eppendorf) until further usage ...
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No products found
because this supplier's products are not listed.
Jérôme Wahis, et al.,
bioRxiv - Neuroscience 2023
Quote:
... cells were washed with PBS (3 x 5 minutes) and mounted on standard microscope glass slides using DAPI-Fluoromount G (SouthernBiotech, 0100-20). Antibodies and dilutions used were as follows ...
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No products found
because this supplier's products are not listed.
Nioosha Nekooie-Marnany, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Cy-3 or Cy-5 (Jackson Immunoresearch Laboratories), and processed for DAPI or Hoechst staining to visualize cells’ nuclei before mounting in ImmuMount medium (Shandon) ...
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No products found
because this supplier's products are not listed.
Joel Rüegger, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... or a Waters Acquity UPLC system (equipped with a Gemini C18 column (150×21 mm, 5 µm particle size, Phenomenex) coupled to a SQ mass spectrometer) ...
-
No products found
because this supplier's products are not listed.
Bret Sanders, et al.,
bioRxiv - Genetics 2021
Quote:
... Coverslips were transferred into the recording chamber maintained at RT (20-21°C) on the stage of an Olympus BX61W (Olympus) differential interference contrast (DIC ...
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No products found
because this supplier's products are not listed.
Aniruddha Das, et al.,
bioRxiv - Neuroscience 2022
Quote:
... A 3×5 mm2 craniotomy was drilled (Omnidrill35, World Precision Instruments) over an area covering the monocular and binocular primary visual (V1m and V1b ...
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Prepared to contain higher collagenase and caseinase activities. A dialyzed, lyophilized powder.
Cat# LS005282,
1 gm, $246.00
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Katherine L. Leiby, et al.,
bioRxiv - Bioengineering 2022
Quote:
... and then inflated via the trachea with 2-3 mL of pre-warmed (37°C) enzyme solution (1 mg/mL collagenase/dispase [Roche], 3 U/mL elastase [Worthington], 20 U/mL DNase [Worthington] in DMEM). The heart and large airways were dissected away and the remaining lung tissues and enzyme solution were collected and transferred to a shaking 37°C water bath at 75 rpm for 20 min ...