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Citations for Zymo Research :
1 - 50 of 1048 citations for 3 hydroxy 20 oxopregn 5 en 21 yl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA from six whole 20-21 dpf offspring (3 control offspring, 3 treatment offspring) was extracted using a Zymo Duet extraction kit (Zymo, NZ). The integrity of RNA samples was determined using an Agilent RNA 6000 Nano chip on an Agilent 2100 Bioanalyzer to check that the samples had an RNA Integrity Number (RIN ...
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bioRxiv - Genomics 2023Quote: ... cytosines in 87bp DNA fragment were modified with hydroxy-methylation by PCR amplification with 5-hydroxymethyl-dCTP supplement (ZYMO). The hydroxy-methylated DNA fragment was combined with equal amounts of CpG methylated pUC19 DNA (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... using the following two primer sequences (T7 Forward: 5’-TAATACGACTCACTATAGCGTCATC-3’; Reverse: 5’-TTGTCGCACGTTCGGTGTCG-3’) and purified with DNA Clean and Concentrator-5 Kit (Zymo Research, 11-302). dsDNA was converted to RNA with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB ...
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Spatial 3D genome organization controls the activity of bivalent chromatin during human neurogenesisbioRxiv - Neuroscience 2024Quote: ... IPC and EN nuclei using Quick-RNA FFPE RNA extraction kit (Zymo Research, R1008). Total RNA from NPCs was extracted using Direct-Zol RNA Miniprep kit (Zymo Research ...
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bioRxiv - Genetics 2022Quote: ... cerevisiae temperature-sensitive strain TSA64 en masse using EZ Kit Yeast Transformation kit (Zymo Research). The entire transformed library was grown in selective media (YPD + clonNAT ...
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bioRxiv - Immunology 2024Quote: ... and 3×104-5×104 cells were collected for RNA isolation (RNA Clean & Concentrator-5, Zymo # R1013). The isolated RNA was hybridized with a nCounter custom probe set (108 probes ...
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bioRxiv - Bioengineering 2024Quote: ... The reaction was stopped by the addition of Proteinase K (+5 μL 20 mg/mL Proteinase K (Zymo, D3001-2-20), incubated at 56°C for 10 min) ...
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bioRxiv - Bioengineering 2024Quote: ... The reaction was stopped by the addition of Proteinase K (+5 μL 20 mg/mL Proteinase K (Zymo, D3001-2-20), incubated at 56°C for 10 min) ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl RNA from total or immunopurified ribosomes were 3’ dephosphorylated and 5’ phosphorylated as described above 30 and was selectively recovered with RNA Clean and Concentrator-5 (Zymo) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... at a ratio of 1:3 (DNA : Zymo Shield / 20 µl DNA:60 µl Zymo Shield). O volume of 5µl of the PCR product was loaded on a gel for confirmation of the reaction ...
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bioRxiv - Synthetic Biology 2022Quote: ... at a ratio of 1:3 (DNA : Zymo Shield / 20 µl DNA:60 µl Zymo Shield). O volume of 5µl of the PCR product was loaded on a gel for confirmation of the reaction ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 20 μL PCR products were then purified with DNA Clean & Concentrator-5 (Zymo Research, Irvine, CA, USA). Amplicons were eluted with 25 μL of nuclease-free water (Thermo Scientific) ...
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bioRxiv - Genomics 2021Quote: ... 10 mM magnesium acetate) and purified using ChIP DNA Clean & Concentrator (Zymo Research). Samples were quantified by Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... 10 mM magnesium acetate) and purified using ChIP DNA Clean & Concentrator (Zymo Research). Sample concentrations were quantified by Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... and stored at -20°C until (≤ 3 days) the DNA was extracted via the Zymo Quick-DNA Fecal/Soil Microbe kit (Zymo Research) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... DNA was eluted using a DNA Clean & Concentrator Kit in 21 μl elution buffer (Zymo Research #D4013). ATAC-seq libraries were prepared using the NEBNext High-Fidelity 2X PCR Master Mix (NEB M0541 ...
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bioRxiv - Microbiology 2020Quote: ... Most of the supernatant was removed and 750 μl of ZymoBIOMICS Lysis Solution and 19 μl of proteinase K (D3001-2-20/D3001-2-5, Zymo Research) were added to the remaining 200 μl of the samples and incubated for 30 min at +55 °C ...
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bioRxiv - Microbiology 2022Quote: ... were incubated in a total volume of 20 µL for 30 min at 37°C and subsequently column-purified using RNA Clean and concentrator 5 (Zymo Research).
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bioRxiv - Microbiology 2020Quote: ... and mRFP1 was PCR amplified using primers listed in Table 3 and purified using DNA Clean and Concentrator-5 kit (Zymo Research). pUC19 was digested with PstI and pBAD18 was digested with EcoRI and HindIII ...
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bioRxiv - Developmental Biology 2020Quote: RNA was extracted from at least two biological replicates for each sample of cells at days 3 and 5 of differentiation using Direct-zol RNA extraction kit (Zymo Research). mRNA was captured using NEBNext (Poly A ...
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bioRxiv - Cancer Biology 2023Quote: ... change of reverse transcription primers for compatibility with standard Illumina sequencing primers 3) cDNA clean-up prior to library amplification performed with the DNA Clean & Concentrator-5 kit (Zymo, #D4013) 4 ...
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bioRxiv - Neuroscience 2020Quote: ... 1 ml Proteinase K (20 mg, Zymo D3001-2-20), 9 mL water ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR reactions containing the dsDNA block were cleaned and concentrated to 20 μl mQ using the DNA Clean & Concentrator™-5 kit (Zymo, D4004). 4 μl Gel Loading Dye ...
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bioRxiv - Bioengineering 2024Quote: ... and ATi disks for 21 days were extracted using the Quick RNA Miniprep kit (Zymo Research, Inc. Irvine, CA). Total RNA samples from 3 separate wells in each group were combined in equal quantities ...
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bioRxiv - Molecular Biology 2021Quote: ... to block the 3’-ends from ligation and were then size-selected to 200 ≥ nt (DNA Clean & Concentrator™-5, Zymo Research). 5’ adaptors were attached using T4 RNA ligase (Ambion ...
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bioRxiv - Genetics 2023Quote: ... Cells were cultured for 3-5 days post-transfection after which genomic DNA was extracted using the Quick-DNA Microprep Kit (Zymo Research Corporation).
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bioRxiv - Physiology 2023Quote: ... repeat 3-5 times on ice) and total RNA was extracted following the instructions of the Quick- RNA MiniPrep kit (Zymo Research, R1055) and reverse transcription was performed by SuperScript™ III (18080093 ...
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bioRxiv - Genetics 2023Quote: ... 30 samples were collected (5 timepoints x 2 genotypes x 3 replicates) and RNA was extracted using a Direct-zol RNA Miniprep (Zymo Research R2061) using TRIzol Reagent (ThermoFisher Cat # 15596026 ...
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bioRxiv - Microbiology 2020Quote: ... Strains carrying the pLacZ plasmid appear blue when plated on 0.008% X-gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Zymo Research, CA, USA). X-gal concentrations above 0.008% appear to inhibit growth of S ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted from C2C12 myoblasts or postnatal day 21 tail tip samples using Direct-zol RNA miniprep kit (Zymo Research) and reverse-transcribed using High Capacity cDNA Reverse Transcription kit (Applied Biosystems ...
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bioRxiv - Biochemistry 2023Quote: A standard whole-cell mixture consisting of 21 representative strains from 17 species of the gut microbiota (Zymo Research, ref D6331) was divided into 10 aliquots and stored in the storage solution provided by the manufacturer at −80 °C ...
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bioRxiv - Bioengineering 2024Quote: ... 1 mL of suspended biomass was collected in triplicate from the mixed tank and PBR effluent and stored in 5 mL polypropylene transport tubes (FisherbrandTM, USA) prefilled with 3 mL DNA/RNA ShieldTM (Zymo Research corp., CA, USA). The samples were briefly vortexed and stored at -20 °C until they were shipped to University at Buffalo (UB ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction mixture was incubated at 65 °C for 3 h and the RNA was then isolated by using the Zymo RNA clean and concentrator-5 kit (Zymo Research, cat. no. R1013) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2022Quote: ... the second sample was stored at RT for 3 – 5 days and the third sample was collected in a Zymo research tube (Zymo Research Inc., Irvine, CA, USA) containing 9ml DNA/RNA shield and stored at RT for 3 – 5 days before storage at −80 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Products from PCR1 were run on a 2% agarose gel in TAE (Tris-acetate-EDTA) buffer and fragments were extracted using the Zymo Gel DNA recovery Kit (Zymo). Eluted products were pooled in equimolar amounts and were submitted to the UC Davis DNA Technologies and Expression Analysis Core lab for a second round of PCR (PCR2 ...
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bioRxiv - Microbiology 2022Quote: ... and the desired 4671 bp and 7177 bp fragments containing either RM_p16 to 20 (pRM bp 14561-19231) or RM_p16 through 21 (pRM bp 14561-21646) were gel-purified (Zymoclean ™Gel DNA Recovery Kit, Zymo Research, Irvine, CA). The fragment ends were blunted (DNATerminator® ...
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bioRxiv - Cell Biology 2024Quote: Yeast transformations were performed either using a standard lithium acetate transformation or Zymo Frozen-EZ Yeast Transformation II Kit (Zymo #T2001) before plating on appropriate selection media82 ...
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bioRxiv - Bioengineering 2021Quote: ... Selection for pyrF mutations was performed with 5-fluoroorotic acid (5-FOA, Zymo Research, part number F9001-5) at a final concentration of 0.5 mg/ml ...
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bioRxiv - Genomics 2023Quote: ... 20 µL of DNA binding buffer (Zymo) was added to each well and incubated at room temperature for 20 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... the shuffle strain transformed with plasmid expressing mutant ribosomal protein products was grown on SDC plates containing 0.5 mg/mL 5-fluoroorotic acid (5-FOA, #F9001-5, Zymo Research) and a strain lacking URA3 was isolated.
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bioRxiv - Plant Biology 2023Quote: ... Relative quantification of 5-methylcytosine (5-mC) over time was determined using the 5-mC DNA ELISA Kit (Zymo Research), using 100 ng of genomic DNA per sample as input ...
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bioRxiv - Genomics 2024Quote: ... 5 µL DNA Digestion Buffer and 5 µL DNase I (Zymo Research; R1013) was added ...
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bioRxiv - Cancer Biology 2022Quote: ... was used for the estimation of global 5-hmC and 5-mC levels by ELISA using the Quest 5-hmC ELISA kit and the 5-mC DNA ELISA kit (Zymo research Inc, USA) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: OE from 3 controls and 3 sFlt1 mice was dissected and immediately placed in RNA lysis buffer (Zymo Research R1051) without vortexing for 5min and further processed according to manufacturer’s guidelines ...
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bioRxiv - Synthetic Biology 2022Quote: ... 20 µl of each module (PCR product + Zymo Shield) (6 in total ...
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bioRxiv - Cell Biology 2024Quote: ... 10µg of proteinase K (Zymo Research, D3001-2-20) was added to this input sample followed by an overnight incubation at 65°C ...
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bioRxiv - Biochemistry 2023Quote: ... or a combination of TRIzol and the RNA Clean & Concentrator-5 (RCC-5) kit (Zymo Research), according to the Zymo instructions for combination Trizol/Zymo-Spin-Column RNA isolation ...
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bioRxiv - Genomics 2024Quote: Neutralization Buffer (Zymo Research, cat. no. D4027-3-50)
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bioRxiv - Systems Biology 2023Quote: ... 600 µL DNA/RNA wash buffer (Zymo, #D7010-3), and 400 µL DNA/RNA wash buffer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.075% 5-fluorooritic acid (Zymo Research)) ...