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Citations for Leica :
1 - 50 of 1475 citations for 3 hydroxy 20 oxopregn 5 en 21 yl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Grids were post-stained for 30 min with 2% uranyl acetate (Roth) and 20 min with 3% lead citrate (Leica). TEM images were acquired using a Zeiss Leo 912 Omega (Zeiss ...
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bioRxiv - Cell Biology 2020Quote: Sample blocks were obtained by freeze substitution of the cell pellets in 0.5 % uranyl acetate diluted from a 20 % stock in methanol and 3 % double distilled H2O in acetone in a Leica AFS1 (Leica Microsystems GmbH, Wetzlar, Germany). Temperature was set to −130 °C for the first 2 hours and then raised to −90 °C with a 20 °C/hr gradient ...
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bioRxiv - Neuroscience 2020Quote: ... heads were sectioned into 20 µm slices (at -21 °C) using a Cryotome CM1900 (Leica) and mounted on Superfrost Plus slides (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... the heads were sectioned into 20 μm slices (at −21°C) using a cryotome CM1900 (Leica) and mounted on Superfrost Plus slides (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... freeze-substituted in acetone / 1% OsO4 / 5% H2O / 0.25% uranyl acetate (AFS2, Leica, Wetzlar, Germany) and embedded in Epon ...
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bioRxiv - Bioengineering 2023Quote: ... and 20 min in 3% lead citrate (Leica, Germany). TEM images were acquired with a 200 kV TEM JEM 2100Plus (Jeol ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were freeze substituted in acetone containing 0.1% uranyl acetate plus 3% distilled water in a freeze substitution unit (Leica EM AFS2 ...
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bioRxiv - Cell Biology 2022Quote: ... After subsequent freeze substitution (using acetone, containing 0.25% osmium tetroxide, 0.2% uranyl acetate, 0.05% ruthenium red, and 5% water) (Leica AFS2), cells were embedded in Epon812 substitute resin (Fluka) ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were transduced with LV-Syn1-GFP at DIV 3 and were imaged at DIV 21 using an inverted spinning disk confocal microscope (Leica DMi8, 63x objective). During image recording the cells were maintained in a humidified atmosphere of 5% CO2 at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... and cell monolayers were sectioned ‘en face’ at 70 nm thickness using a Reichart Ultracut S ultramicrotome (Leica) and a diamond knife (Diatome) ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were freeze substituted in acetone containing 0.1% uranyl acetate plus 3% distilled water in a freeze substitution unit (Leica EM AFS2, Leica Microsystems, Buffalo Grove, IL) at −90°C for 20 hours ...
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bioRxiv - Developmental Biology 2020Quote: En face STED images of mouse cephalic explants were taken with a Leica SP8 TCS STED microscope (Leica Microsystems) equipped with a pulsed white-light excitation laser (WLL ...
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bioRxiv - Cancer Biology 2020Quote: ... diluted in Novolink DAB Substrate Buffer (Leica Biosystems, 1:20, 5 min). Finally ...
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bioRxiv - Developmental Biology 2022Quote: ... embryos were paraffin-embedded and serially sectioned at 3-5 µm (Leica microtome), before attachment to poly-L-lysine-coated glass slides (Sigma ...
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bioRxiv - Immunology 2023Quote: ... and stained with uranyl acetate and lead citrate (Leica AC20). Micrographs were collected on a JEM1010 (JEOL ...
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bioRxiv - Neuroscience 2024Quote: Ultrathin sections were cut ‘en face’ to the plane of the monolayer using a diamond knife mounted on a Leica Ultracut UC7 ultramicrotome (Leica, Milton Keynes, UK), transferred to EM grids and stained with uranyl acetate and Reynold’s lead citrate ...
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bioRxiv - Neuroscience 2024Quote: ... and stained in uranyl acetate and lead citrate (Leica EM AC20). Sections were imaged in a JEOL1010 transmission electron microscope (JEOL ...
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bioRxiv - Cell Biology 2024Quote: ... The cell monolayer was sectioned en face to the substrate with a diamond knife and Leica UCT Ultramicrotome (Leica Microsystems, Inc, Buffalo Grove, IL). Ultrathin sections (70 nm ...
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bioRxiv - Neuroscience 2024Quote: ... Videos of Day 3 adult worms for at least 20 seconds was recorded using stereo-zoom microscope (Leica) 28 ...
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bioRxiv - Plant Biology 2021Quote: Confocal microscope (Leica TCS LSI macroscope with Plan APO 5 x and 20 x objective, Leica Microsystems) was used to detect emission of chlorophyll or redox state sensitive GFP (roGFP ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were stained in 2% aqueous uranyl acetate and lead citrate (Leica) and imaged on a Hitachi HT7800 transmission electron microscope ...
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bioRxiv - Cell Biology 2022Quote: ... After automated staining with uranyl acetate and lead citrate (Leica EM AC20), samples were observed via either Zeiss TEM 902 A (50 kV ...
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bioRxiv - Cancer Biology 2023Quote: ... stained with uranyl acetate and lead citrate in an EM Stainer (Leica), and examined using a JEM 1010 transmission electron microscope (JEOL ...
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bioRxiv - Plant Biology 2023Quote: ... post-stained with uranyl acetate and lead citrate with an EMSTAIN (Leica) and observed with a Libra 120 transmission electron microscope (Carl Zeiss Microscopy GmbH ...
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bioRxiv - Plant Biology 2022Quote: ... 21 dpi and were frozen in a high-pressure freezer (EM-ICE; Leica, Vienna, Austria) by using the fixation solution (0.25% (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then sliced into 55 μm sections at −21°C using a cryostat (Leica).
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bioRxiv - Neuroscience 2021Quote: ... and sections post-stained with 0.5% uranyl acetate (Leica Ultrostain I, Leica Microsystems) and 3% lead citrate (Leica Ultrostain II ...
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bioRxiv - Neuroscience 2021Quote: ... and sections post-stained with 0.5% uranyl acetate (Leica Ultrostain I, Leica Microsystems) and 3% lead citrate (Leica Ultrostain II ...
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bioRxiv - Bioengineering 2023Quote: ... were afterwards stained for 30 min in 1% aqueous uranyl acetate (Leica, Germany) and 20 min in 3% lead citrate (Leica ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were rinsed in tap water and dipped 3-5 times in 0.5% Acid Alcohol (Leica Biosystems 3803651), followed by rinsing in tap water and bluing with Scott’s Tap Water (Electron Microscopy Sciences 2607007 ...
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bioRxiv - Zoology 2023Quote: ... Paraffin blocks were sectioned (5-7 μm, 20-40 sections per block) using a microtome (Leica RM 2125RT, Germany). Histological sections were stained with hematoxylin and eosin ...
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bioRxiv - Neuroscience 2024Quote: ... 20 mM choline bicarbonate) saturated with carbogen (5% CO2 and 95% O2) using a vibratome (Leica VT 1200 S). 250 μm brain slices were incubated in 31°C ACSF solution (119 mM NaCl ...
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bioRxiv - Immunology 2024Quote: ... and single-cell quantification was performed in 3–5 fields imaged with the ACS_APO 20x/NA 0.60 objective of a confocal microscope (Leica, SPE). Mean Fluorescence Intensity (MFI ...
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bioRxiv - Immunology 2022Quote: ... 3′-3-diaminobenzidine (DAB) substrate (Leica Microsystems) was used as a chromogen followed by hematoxylin counterstain ...
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bioRxiv - Cancer Biology 2021Quote: ... endogenous peroxidase activity was blocked by incubation with 3% peroxide block for 20 minutes (BOND Polymer Refine Detection System, Leica, Germany; DS9800). The sections were blocked with 10% normal goat serum (Vector ...
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bioRxiv - Neuroscience 2023Quote: ... were rinsed in PBS and embedded in 3% agarose containing 1% Tween 20 and sectioned at 300 µm in the frontal plane with a vibratome (Leica VT 1200S). Under a stereomicroscope (Olympus SZX7) ...
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bioRxiv - Biochemistry 2020Quote: ... Protein mixture (5 μL) were deposited onto the grids and blotted for 3 seconds using an Automatic Plunge Freezer (Leica).
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bioRxiv - Immunology 2021Quote: ... The fixed specimens were embedded directly in paraffin blocks and cut into sections with 3–5 μm thickness using a rotary microtome (Leica RM 2125RTS, Leica Biosystems Nussloch GmbH ...
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bioRxiv - Immunology 2021Quote: ... The fixed specimens were embedded directly in paraffin blocks and cut into sections with 3–5 μm thickness using a rotary microtome (Leica RM 2125RTS ...
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bioRxiv - Cell Biology 2020Quote: ... Bright field images of beating cilia were captured at 160 frames per sec over 4 seconds (3-5 videos per insert, Leica Spinning disc confocal with 40x water objective) ...
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bioRxiv - Genetics 2021Quote: Assessment of Aβ deposition was carried out by immunohistochemical staining of paraffin-embedded sections (3–5 μm-thick) with a mouse monoclonal (S6 F/3D) anti-Aβ antibody (Leica Biosystems Newcastle Ltd ...
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bioRxiv - Developmental Biology 2024Quote: ... Live VL3/VL4 NMJs in abdominal hemi-segment 3 were imaged in ambient temperature using a Stellaris 5 laser-scanning confocal microscope (Leica) with a water-immersion objective (HC FLUOTAR L VISIR 25x/0.95 ...
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bioRxiv - Biophysics 2024Quote: ... Grids were blotted for 3-5 seconds then plunge frozen into liquid ethane cooled by liquid nitrogen using an EM GP2 (Leica) plunge freezer at 4°C and 100% relative humidity ...
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bioRxiv - Cancer Biology 2023Quote: ... at a final concentration of 5 µg/mL for 20 minutes at 37 °C prior to live cell imaging by epifluorescence (Leica DMI6000B). Cells were counted as DAPI-positive if the nucleus showed bright staining and as apoptotic if there was characteristic nuclear fragmentation ...
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bioRxiv - Cancer Biology 2022Quote: ... was incubated at 100 degrees Celsius for 20 minutes (for anti-Cytokeratin 5, Bond Epitope Retrieval 1, pH6.0 (Leica Biosystems, AR9961) was used instead) ...
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bioRxiv - Microbiology 2024Quote: ... at 17°C and 63.5 bar and the sample was sputter coated with a 5 nm thick layer of Au/Pd (80/20) using the Leica ACE600 (Leica Microsystems). Samples were mapped by SEM using an Aquilos 2 dual-beam cryo-focused ion beam-scanning electron microscope (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The glycolytic and oxidative portions of the TA muscles of 5 mice in the CTR group and 5 mice in the Crush - 3 days group were microdissected with a laser microdissection system (Leica LMD6) and recovered in RNAlater reagent (QIAGEN).
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bioRxiv - Neuroscience 2021Quote: ... 20 and 20 µm thickness respectively using a cryostat (Leica). Immunohistochemistry on tissue sections will be performed according to standard procedures ...
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bioRxiv - Developmental Biology 2021Quote: ... a z-stack (1 µm sections) was acquired at 21–22 °C with the HC PL APO 63×/1.40 NA oil immersion objective (Leica).
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bioRxiv - Neuroscience 2020Quote: ... Slices were prepared in oxygenated (95% O2 / 5% CO2) dissection artificial cerebrospinal fluid (ACSF) at low temperature (3-4°C) using vibroslicer (Leica, VT 1200S). After preparation slices were recovered in a resting chamber (Harvard apparatus ...