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Citations for Sartorius :
1 - 50 of 409 citations for 3 hydroxy 20 oxopregn 5 en 21 yl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The proteins were spin concentrated to 5 mg / ml (3 kDa MWCO, vivaspin 20, Sartorius), aliquoted ...
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bioRxiv - Genetics 2023Quote: ... The eluate was washed 3 times with PBS containing 5% Sorbitol and concentrated with Vivaspin 20 100K concentrator (Sartorius Stedim, Bohemia, NY). Vector titer was determined by qPCR with primers and labeled probe targeting the ITR sequence (Aurnhammer et al. ...
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bioRxiv - Synthetic Biology 2022Quote: ... The supernatant was then filtered with a Minisart® 5 μ m cellulose acetate (SFCA) filter (Sartorius) before being mixed 1 ...
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bioRxiv - Microbiology 2024Quote: ... Cytiva) in 50 mM sodium acetate (pH 5.2) and concentrated using Vivaspin polyethersulfone membrane (3 kDa cut-off, Sartorius). Protein concentrations were determined using the Bradford method (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... The UGT2B15 promoter 20 base-pair sequence (5’-TAACTTGATTGATTTTTCCT-3’ for wild type and 5’-TAACTTGGCTGTCTTTTCCT-3’ for mutant) was immobilized to a streptavidin SADH sensor chip (Sartorius) in running buffer (10 mM HEPES pH 6.8 ...
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bioRxiv - Microbiology 2021Quote: ... 3 L of water were pre-filtered through the 5 μm filter (Sartorius). The flow-through was further filtered using the 0.2 μm filter (Sterivex) ...
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bioRxiv - Genomics 2021Quote: ... Each diluted blood sample was then filtered using a 5 μm surfactant-free cellulose acetate syringe filter (Minisart NML® syringe filter, Sartorius reference number: 17594K) connected to a 10 mL syringe (Figure 1) ...
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bioRxiv - Microbiology 2023Quote: ... The filtered medium was then concentrated by ultrafiltration using Vivaspin 20 (3 kDa cut-off, Sartorius). Protein concentration was determined using a BCA protein assay kit (Beyotime ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 mM of IncuCyte Caspase-3/7 Green Apoptosis Assay Reagent (Sartorius, cat #4440) as well as 1:500 of Nuclight Rapid Red Dye (Sartorius ...
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bioRxiv - Cancer Biology 2021Quote: ... and 5 μM Caspase 3/7 dye was included in the media (Sartorius #4704). The number of red nuclei (indicating active caspase 3/7 ...
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bioRxiv - Biophysics 2024Quote: ... Collected infected cells were passed three times through a 25G needle and filtered through a 5-μm cellulose acetate filter (Sartorius, Cat#S7594-FMOSK, Göttingen, Germany). Isolated parasites were washed three times with Endo solution using a 10 min centrifugation at 400 x g and resuspended in 50 µL Endo solution for electrophysiological experiments ...
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bioRxiv - Plant Biology 2020Quote: ... eggs were placed on a cellulose–acetate filter membrane (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Bioengineering 2024Quote: ... The purified protein from elutions containing the fewest contaminating proteins (typically elution steps 3-5) were concentrated in 1× PBS using a 5 kDa cut-off Vivaspin Centrifugal Concentrator (Sartorius, Oakville, Ontario, Canada). Protein concentrations were determined using the micro-BCA assay (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... appropriate fractions were pooled and concentrated to 5 mL with centrifugal filter devices (Vivaspin 20, Sartorius). In pHisSUMO-rnSTAT2 preparations ...
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bioRxiv - Neuroscience 2020Quote: ... the iodixanol was removed from the AAV vector containing fraction by 3 rounds of diafiltration using Vivaspin 20 ultrafiltration devices (100’000 MWCO, PES membrane, Sartorius) and 1x phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2021Quote: ... pooled and filtered with a 0.45 µm cellulose acetate syringe filter (Sartorius), subsequently overlaid onto 5 mL of 20% sucrose and ultracentrifuged at 24,000 rpm for 2.5 hrs (Beckman XPN80 ...
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bioRxiv - Microbiology 2021Quote: ... 200 mL water were filtered through 0.45 μm cellulose acetate filters (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Biochemistry 2024Quote: ... and appropriate fractions were pooled and concentrated to 5 mL with centrifugal filter devices (Vivaspin 20, Sartorius). Subsequently ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM dithiothreitol (DTT)) at 4°C overnight and concentrated to ~3-5 mg/mL using Vivaspin 6 concentrators (Sartorius, 6 ml, 100 kDa cut-off). Mammalian septin hexamers composed of mouse Sept2 (98.6% identical to human Sept2 ...
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bioRxiv - Biochemistry 2020Quote: ... Unreacted propargylamine was removed by size exclusion chromatography and Ub/LC3B-PA was concentrated using VIVASPIN 20 Columns (3 kDa cutoff, Sartorius), flash frozen and stored at −80 °C.
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bioRxiv - Biochemistry 2023Quote: ... Unreacted propargylamine was removed by size exclusion chromatography and the probes were concentrated using VIVASPIN 20 Columns (3 kDa cutoff, Sartorius), flash frozen and stored at −80 °C ...
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bioRxiv - Biochemistry 2024Quote: ... Unreacted propargylamine was removed by size exclusion chromatography and the probes were concentrated using VIVASPIN 20 Columns (3⍰kDa cutoff, Sartorius), flash frozen and stored at −80⍰°C.
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bioRxiv - Evolutionary Biology 2024Quote: ... Filtered HES was concentrated using a VivaSpin 20 centrifugal concentrator with a 5 kDa molecular weight cutoff (Sartorius). EVs were purified from concentrated HES by ultracentrifugation at 100,000 x g for 90 mins at 4 °C in polyallomer tubes (Beckman Coulter ...
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bioRxiv - Biochemistry 2024Quote: ... The flow-through fraction was collected and concentrated to 5 mL with centrifugal filter devices (Vivaspin 20, Sartorius). A second SEC was performed on an Äkta prime plus FPLC (Cytiva) ...
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bioRxiv - Microbiology 2020Quote: ... Samples were then immediately passed through a 0.2 μm cellulose acetate filter (Sartorius) and collected in a microcentrifuge tube ...
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bioRxiv - Microbiology 2023Quote: ... Cell suspensions were immediately passed through 0.2 µm syringe filters (cellulose acetate, Sartorius) to separate conidia from the digestion buffer followed by washing the filters with 200 µl of 25 mM AB ...
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bioRxiv - Microbiology 2022Quote: ... 5 mM MgCl2) and concentrated to ~1 mg/mL by Vivaspin 20 centrifugal filter unit (MWCO 10 kDa) (Sartorius). Sfp synthase and CoA-conjugated dyes were prepared as previously described54 and used to perform the overnight Fab labeling reaction on ice ...
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bioRxiv - Biophysics 2023Quote: ... using an acquisition rate of 5 Hz averaged by 20 using an Octet© R8 BLI System (FortéBio/Sartorius), with assays designed and setup using Blitz Pro 1.3 Software ...
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bioRxiv - Biophysics 2024Quote: ... using an acquisition rate of 5 Hz averaged by 20 using an Octet© R8 BLI System (FortéBio/Sartorius), with assays designed and setup using Blitz Pro 1.3 Software ...
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bioRxiv - Cancer Biology 2024Quote: ... and ABTR#2 cells were infected at an MOI of 3-5 with the IncuCyte Nuclight Red (#4476 Sartorius) or IncuCyte Cell Cycle Green/Red Lentivirus Reagent (#4779 ...
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bioRxiv - Genomics 2020Quote: ... and a donor-recipient mixture were deposited on 0.2–μm cellulose acetate filters (Sartorius) placed on MM agar plates ...
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bioRxiv - Microbiology 2021Quote: ... This mixture was deposited on top of a 0.2–μm cellulose acetate filter (Sartorius) placed on MM-agar containing 0.5 mM 3CBA ...
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bioRxiv - Microbiology 2024Quote: ... and a donor-recipient mixture were deposited on 0.2–µm cellulose acetate filters (Sartorius) placed on MM succinate agar plates ...
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bioRxiv - Biophysics 2022Quote: BLI analyses were performed at 25°C at an acquisition rate of 5 Hz averaged by 20 using an Octet© BLItz System (FortéBio/Sartorius) with assays were designed and setup using Blitz Pro 1.3 Software ...
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bioRxiv - Biochemistry 2021Quote: The dialysed proteins were concentrated to a <5 mL volume using a 30 kDa MWCO Vivaspin 20 centrifugal concentrator (Sartorius) at 5000 g ...
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bioRxiv - Developmental Biology 2024Quote: ... for a total of 15-20 minutes with trituration every 5 minutes using 200 μl low bind tips (Sartorius, 790101F). Papain was inactivated using an Ovomucoid inhibitor (Worthington Papain Dissociation Kit ...
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bioRxiv - Cancer Biology 2023Quote: ... fractions containing the protein of interest were pooled together and concentrated using Vivaspin 20 centrifugal concentrators with a 5 kDa cut-off (Sartorius). The concentration of BRD4 BD1 was determined spectrophotometrically at 280 nm using the calculated molar extinction coefficient ...
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bioRxiv - Cancer Biology 2023Quote: ... fractions containing FKBP12 F36V were pooled together and concentrated using Vivaspin 20 centrifugal concentrators with a 5 kDa cut-off (Sartorius). The concentration of FKBP12 F36V was determined spectrophotometrically at 280 nm using the calculated molar extinction coefficient ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The supernatant (EV-depleted HES) was removed and concentrated using a VivaSpin 20 centrifugal concentrator with a 5 kDa molecular weight cutoff (Sartorius). Purified EVs were washed twice with PBS (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... The DNA duplex was captured at a concentration of 1.25 µg/ml in BLI Buffer (ELB containing 0.05% Tween-20 and 5 mg/ml BSA) on an Octet® Streptavidin (SA) Biosensor (Sartorius). His-tagged X ...
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bioRxiv - Biophysics 2023Quote: ... or PPIase) were buffer-exchanged into an NMR buffer using either 5 or 3 kDa MWCO centrifugal concentrators (Vivaspin, Sartorius). The NMR buffer contained 10 mM d4-imidazole at pH 6.6 ...
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bioRxiv - Molecular Biology 2023Quote: ... 3–5 x103 cells were seeded in 96-well plates and viable cell numbers were determined using the Incucyte (Sartorius) Adherent-Cell-by-Cell module ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 x 104 WM852 or 5 x 104 WM793 cells were seeded in a 96-well ImageLock plate (Sartorius, 4379) and allowed to form a monolayer for 24 h prior to the assay ...
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bioRxiv - Immunology 2020Quote: ... 0.5 µg of AviTagged HA diluted in acetate 4.5 (pall fortebio, Sartorius group, Amsterdam, Netherlands) was immobilized onto SAX biosensors (high precision streptavidin sensors ...
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bioRxiv - Microbiology 2024Quote: ... and the supernatant was filtered through 0.20-µm pore cellulose acetate syringe filters (Sartorius, Germany). The filtrate was mixed with 0.1 volume of chloroform (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... Protein containing fractions were pooled and concentrated to 5 mg/ml in a Vivaspin 500 (3 kDa) centrifugal concentrator (Sartorius AG), after which they were aliquoted ...
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bioRxiv - Genetics 2021Quote: ... grape juices were sterilized by membrane filtration (cellulose acetate 0.45 μm Sartorius Stedim Biotech, Aubagne, France). Fermentations were carried out as previously described (Peltier ...
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bioRxiv - Microbiology 2023Quote: ... The suspensions were then filtered through 0.45 μm cellulose acetate filters (type Minisart, Sartorius, Göttingen, Germany) to remove a large portion of remaining sponge cells and nuclei (microscopic visualizations showed a lack of sponge nuclei after filtration) ...
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bioRxiv - Microbiology 2024Quote: ... each filter was conditioned for 48h at a constant temperature of 20°C ± 1°C and 50% ± 5% relative humidity values in a controlled environment (SCC 400L Climatic Cabinet, Sartorius, Varedo (MB), Italy) ...
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bioRxiv - Microbiology 2020Quote: ... Biomass was finally concentrated on 0.22 μm pore-size cellulose acetate filters (Sartorius 11107-142-N, Germany) using a peristaltic pump ...