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Citations for Jackson ImmunoResearch :
1 - 50 of 668 citations for 3 hydroxy 20 oxopregn 5 en 21 yl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... Cy-3 or Cy-5 (Jackson Immunoresearch Laboratories), and processed for DAPI or Hoechst staining to visualize cells’ nuclei before mounting in ImmuMount medium (Shandon) ...
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bioRxiv - Neuroscience 2024Quote: ... aminomethylcoumarin acetate (Jackson ImmunoResearch Labs), goat anti-mouse and rabbit conjugated to horseradish peroxidase (Jackson ImmunoResearch Labs).
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bioRxiv - Neuroscience 2021Quote: ... Cy-3 or Cy-5 (1:500, Jackson ImmunoResearch). Sections were counterstained with a fluorescent Nissl stain (NeuroTrace 435/445 blue ...
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bioRxiv - Microbiology 2021Quote: ... 5% BSA and 20% Normal Goat Serum (Jackson ImmunoResearch Laboratories, Inc) for 1 hour at room temperature on a rocker ...
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bioRxiv - Developmental Biology 2023Quote: ... Subsequently the coverslips were washed three times in wash buffer (0.05% Tween-20 in PBS) for 5 minutes and blocked for 20 minutes in 200 ml wash buffer plus 5% normal donkey serum (Jackson ImmunoResearch Laboratories). The primary antibodies were diluted in 5% normal donkey serum in PBS and incubated with the tissues 1 hour at room temperature followed by three washes in wash buffer for 5 minutes each ...
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bioRxiv - Cell Biology 2020Quote: ... and blocked for 20 min in PBS supplemented with 5% normal donkey serum (Jackson Immunoresearch) and 3% Bovine Serum Albumin (BSA) ...
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bioRxiv - Immunology 2022Quote: 20 μL anti-IgG AlexaFluorophore 647 mAb (5 μg /mL) (Jackson Immunoresearch 109-605-098) was added to the cells and they were incubated for 45 min at 4 °C and 750 RPM on a thermomixer (Eppendorf ...
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bioRxiv - Physiology 2023Quote: ... Samples were blocked for at least 3 hours in 5% normal goat serum (NGS; Jackson ImmunoResearch) and 5% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed in TBST (TBS with 0.05% Tween 20) for 30 min (3 × 10 min) and incubated with secondary antibodies conjugated to horseradish peroxidase (Jackson ImmunoResearch) for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... then permeabilized and blocked with 0.5% Tween-20 and 5% normal donkey serum (Jackson ImmunoResearch, 017-000-121) for 45 min at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were permeabilized with 0.1% Triton X-100 for 3 min and blocked with 5% donkey serum (Jackson ImmunoResearch), followed by incubation with antibodies for 1 hr at room temperature or overnight at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 3-5 h with a secondary antibody (Alexa Fluor® 488 anti rabbit; Cat# 711-545-152, RRID:AB_2313584, Jackson ImmunoResearch, Cambridge ...
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bioRxiv - Neuroscience 2024Quote: ... The floating sections were washed 3×10 min with 1x PBS and blocked with 5% normal donkey serum (Jackson ImmunoResearch), 2% fish gelatin (Thermo Fisher) ...
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bioRxiv - Neuroscience 2021Quote: ... donkey anti-chicken IgY conjugated to Alexa Fluor 647 and Aminomethylcoumarin Acetate (Jackson ImmunoResearch Labs)
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bioRxiv - Neuroscience 2021Quote: ... Selected slices were then blocked for 3 h at room temperature in a PBS solution containing 5% normal donkey serum (NDS; Jackson ImmunoResearch) and 1% of non-ionic detergent (Triton® X-100 ...
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bioRxiv - Microbiology 2021Quote: ... cells were rinsed 3 times for 5 min in PBS and incubated for 30 min with a cyanine 3-conjugated goat anti-mouse secondary antibody (Jackson Immunoresearch) and DAPI (4′,6-diamidino-2-phenylindole).
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bioRxiv - Neuroscience 2024Quote: ... cells were carefully washed 3×5 min with PBS and incubated with secondary antibodies (Cat.# 711-545-152, 711-605-152, Jackson ImmunoResearch) for 1 h at RT ...
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bioRxiv - Immunology 2023Quote: ... sections were incubated with 0.3% Triton-X in PBS (wash buffer) for 30 minutes for permeabilization and then with 5% Normal goat serum (Jackson ImmunoResearch) in wash buffer (blocking buffer) ...
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bioRxiv - Neuroscience 2023Quote: ... washed in PBST 3 times (5 min in each time) and incubated with secondary antibodies (mouse anti-Cy3, Jackson Immunoresearch,1:300) for 1h ...
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bioRxiv - Immunology 2020Quote: ... circumscribed with ImmEdge pen (Vector) and blocked in TBST (100mL Tris-HCl, 150mM NaCl, 0.05% Tween-20) containing 5% Donkey serum (Jackson ImmunoResearch #017-000-121) for 2 hours at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... Sections of 20 um thickness were blocked in PBS + 0.3% Triton X-100 and 5% normal donkey serum (Jackson ImmunoResearch, 017-000-121) for one hour ...
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bioRxiv - Plant Biology 2022Quote: ... the seedlings were washed for three times 5 min each and then incubated in the secondary antibody Cy-3-goat anti-mouse IgG (1:200; Jackson Immunoresearch, http://www.jacksonimmuno.com). The imaging was performed on a Nikon laser scanning microscope (Eclipse Ti equipped with Nikon C2 Si laser scanning unit ...
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bioRxiv - Molecular Biology 2023Quote: ... After washing once in PBS-T (PBS with 0.2% Tween-20), cells were blocked with blocking buffer (PBS-T, 2% BSA, and 5% goat serum (Jackson Immunoresearch/Dianova, 017-000-121) for 1 hour at room temperature ...
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bioRxiv - Immunology 2024Quote: ... or cultured cells stained for the expression of surface markers with specific antibodies in PBS with 5% FBS and 20% normal goat serum (Jackson ImmunoResearch 005-000-121) and 0.01% NaN3 for 20 min on ice ...
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bioRxiv - Developmental Biology 2023Quote: ... After washing once in PBS-T (PBS with 0.2% Tween-20), cells were blocked with blocking buffer (PBS-T, 2% BSA and 5% goat serum (Jackson Immunoresearch/Dianova, 017-000-121) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were washed 3 x 5 min with PBS and the secondary antibodies – donkey-anti-mouse Cy3 (1:500, Jackson ImmunoResearch, 715-165-151) and goat-anti-rabbit Alexa Fluor 488 (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed in PBS (3×5 min) and incubated overnight with secondary antibody (peroxidase conjugated donkey-anti-rabbit 1:500, Jackson Immunoresearch, Baltimore, PA, USA). The Tyramide Signal Amplification procedure was then employed ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then incubated at 4°C overnight with primary antibody at 1:100 in blocking buffer The following day cells were washed 3 times for 5 minutes with 1X TBS then incubated with Cy5 Donkey Anti-Mouse (Jackson ImmunoResearch 715-175-150) at 1:100 in 1X TBS for 2 hours in the dark at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed 3×5 min with PBS and stained with 1:1,000 donkey anti-chicken Alexa Fluor 488 secondary antibody (Jackson ImmunoResearch #703-545-155, RRID: AB_2340375), rinsed with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed (3 x 5 min in PBT) and incubated with fluorophore- conjugated streptavidin (Streptavidin AlexaFluor647, Jackson ImmunoResearch, 016-600-084, 1:1000) in 10% NDS in PBT for 2 hours at RT ...
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bioRxiv - Immunology 2024Quote: ... excess primary antibodies were washed off with PBS (3 washes, 5 minutes each) followed by incubation of donkey anti-mouse Cy3 secondary antibody (Jackson Immunoresearch, Cat# 715-167-003) for 1 h at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... + 3% donkey serum (Jackson ImmunoResearch)] at room temperature for 15 mins ...
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bioRxiv - Immunology 2023Quote: ... A coupled lawn of goat anti-human IgG Fc (hFc; 50 µg/mL in 10 mM sodium acetate, pH 4.5) (Jackson ImmunoResearch) was then prepared before quenching with 0.5 M ethanolamine (pH 8.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... The membranes were blocked with 3% nonfat milk and 3% bovine serum albumin (Jackson ImmunoResearch) in Tris-Buffered Saline Tween-20 and probed with antibodies against the cytoplasmic domain of NOTCH4 [23] and β-ACTIN (Abclonal) ...
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bioRxiv - Molecular Biology 2021Quote: ... washed again and blocked in 3% fetal bovine serum/3% normal goat serum (Jackson ImmunoResearch). Primary antibody (CD44 ...
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bioRxiv - Cell Biology 2023Quote: ... samples were then transferred directly into blocking buffer (4% BSA (Sigma Cat# 10735086001)/3% goat serum (Jackson Immunoresearch #005000121)/PBS) ...
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bioRxiv - Genomics 2021Quote: ... 5% normal donkey serum and 5% normal goat serum (Jackson Immunoresearch) for 30 min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... slides were washed 3×3 minutes in PBS then stained with secondary antibody (Jackson ImmunoResearch, 1:500) diluted in the blocking buffer for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... slides were washed 3×3 minutes in PBS then stained with secondary antibody (Jackson ImmunoResearch, 1:500) diluted in PBS for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... and 3% Normal Donkey Serum (Jackson Immunoresearch). Retinas were then stained with primary antibody for 5-7 days at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... and 3% Normal Donkey Serum (Jackson Immunoresearch). Sections were incubated in primary antibodies overnight ...
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bioRxiv - Immunology 2022Quote: ... then blocked with 5% goat serum + 5% donkey serum (both from Jackson Immunoresearch) in PBS for 2 h and stained with primary antibodies ...
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bioRxiv - Immunology 2020Quote: ... 5% goat serum (Jackson Immunoresearch) and 0.1% Triton-X in PBS for 1 hour at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... 5% donkey serum (Jackson ImmunoResearch) and 0.3% Triton X-100 (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... mEos2::HIM-3 was co-stained with a rabbit anti-HIM-3 antibody and a donkey-anti-rabbit secondary antibody (Jackson ImmunoResearch) labelled with CF568-NHS ester to achieve a 2:1 dye-to-antibody ratio ...
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bioRxiv - Physiology 2023Quote: ... containing 0.05% Tween 20 and 0.2% BSA (Jackson ImmunoResearch) and preserved in prediluted form in 60 µl aliquots on ice during blood collection ...
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Cortical synaptic vulnerabilities revealed in a α-synuclein aggregation model of Parkinson’s diseasebioRxiv - Neuroscience 2024Quote: ... then blocked in 20% normal goat serum (Jackson ImmunoResearch) in PBS for 1 h at room temperature (RT) ...
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bioRxiv - Molecular Biology 2020Quote: ... Slides were washed 3 times for 3 minutes in PBST each and incubated in biotinylated rabbit antibody (Jackson Immunoresearch #711-065-152) 1:250 dilution in blocking solution for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... blocked for 2-3 hours (0.3% Triton X-100, American Bioanalytical, Canton, MA; 3% normal donkey serum, Jackson ImmunoResearch, West Grove, PA), then incubated overnight with primary antibodies (1:1000 + 1% normal donkey serum) ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-mouse Cyanine 3 (115-167-003, Jackson ImmunoResearch), anti-rabbit Cyanine 3 (111-166-045 ...