Labshake search
Citations for Takara Bio :
1501 - 1550 of 7163 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... Total RNAs of 0.5 to 1 μg were used as templates for the reverse transcription using PrimeScriptTM RT Reagent Kit with gDNA Eraser (Takara). Quantitative PCR (qPCR ...
-
bioRxiv - Cell Biology 2020Quote: ... 1 μg of total RNA from each condition was converted into cDNA using PrimeScript RT reagent with gDNA Eraser kit (Takara Clontech) and MJ Research PTC-220 Dyad PCR System (Conquer Scientific) ...
-
bioRxiv - Molecular Biology 2022Quote: ... First-strand cDNA was synthesized from the total RNA using a PrimeScript™ RT Reagent Kit with gDNA Eraser (Takara) following the manufacturer’s protocols ...
-
bioRxiv - Microbiology 2022Quote: ... Then RNA was reverse-transcribed and amplified using One Step PrimeScriptTM III RT-qPCR Mix kit (Takara Bio, Kyoto, Japan). Primers and probes ...
-
bioRxiv - Cell Biology 2022Quote: ... For each specimen 500 ng of total RNA was reversed transcribed into cDNA using PrimeScript RT reagent kit (TaKaRa RR037a) and oligo dT primers according to the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2022Quote: ... the gel-purified RNA was reverse transcribed into cDNA using a PrimeScript RT Reagent Kit with random primers (TAKARA, RR037B), followed by PCR with primers that can amplify transcripts across the splice junction ...
-
bioRxiv - Neuroscience 2020Quote: ... Total RNA was extracted from the isolated tissue and was subjected to reverse transcription (RT) with the use of a NucleoSpin RNA extraction kit (U955C, Takara) and PrimeScript RT reagent kit (RR037 ...
-
bioRxiv - Plant Biology 2021Quote: 500 ng of the extracted total RNA were subjected to the first-strand cDNA synthesis with the primescript RT reagent kit (Takara). Quantitative real-time PCR was performed with Thunderbird SYBR qPCR mix (Toyobo ...
-
bioRxiv - Developmental Biology 2022Quote: ... was used to purify RNA and 500 ng RNA was used for reverse transcription with a Prime Script RT Reagent Kit (RR037A; Takara). Quantitative PCR was performed using a TB Green Premix DimerEraser Kit (RR091A ...
-
bioRxiv - Genomics 2019Quote: ... which was further reverse transcribed into cDNA using a PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa, Tokyo, Japan), following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... and then was used for generating the first strand complementary DNA (cDNA) as described in the protocol of the Takara PrimeScript RT reagent Kit with gDNA Eraser (Takara). Briefly ...
-
bioRxiv - Cell Biology 2019Quote: ... First-strand cDNA was synthesized from l μg of RNA using the PrimeScript RT Reagent Kit with gDNA Eraser (catalogue No. RR047A, TaKaRa). qPCR was performed in triplicate in 20-μl reactions containing SYBR Premix Ex Taq II (catalogue No ...
-
bioRxiv - Microbiology 2021Quote: ... extracted RNA was reverse-transcribed into cDNA using the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa, Dalian, China). The gene quantitative PCR was performed using TB green Premix EX Taq (Takara ...
-
bioRxiv - Plant Biology 2021Quote: ... was added to mitigate DNA contamination prior to first-strand cDNA synthesis with PrimeScript™ RT reagent kit (Takara Bio) from approximately 1 g total RNA ...
-
bioRxiv - Molecular Biology 2021Quote: ... Two-point five microliters of the polyadenylated sample was used for cDNA synthesis by the PrimeScript RT reagent kit with gDNA eraser (TaKaRa), with the y625 primer in Table S1 ...
-
bioRxiv - Microbiology 2020Quote: ... Approximately 1,000 ng of RNA was reverse transcribed using the PrimeScript RT Reagent Kit with gDNA Eraser (TaKaRa, Dalian, China) according to the manufacturer’s instructions ...
-
bioRxiv - Pathology 2020Quote: ... Single stranded cDNA was synthesized with the oligo(dT) primer using PrimeScript™ RT reagent Kit with gDNA Eraser (Takara), the obtained cDNAs were analyzed by real-time PCR ...
-
bioRxiv - Molecular Biology 2019Quote: ... Total RNA was reverse transcribed to complementary DNA (cDNA) using a Prime Script™ RT Master Mix Kit (TaKaRa, China) and the following procedure ...
-
bioRxiv - Neuroscience 2020Quote: ... the RNA sample was diluted into 140 ng/μL and purified with the Primescript TM RT regent kit (Takara, RR047). cDNA was generated using SYBR® Premix Ex Taq™ II kit (Takara ...
-
bioRxiv - Pathology 2021Quote: ... First-strand complementary DNA was synthesized from total RNA using a PrimeScriptTM RT reagent kit with gDNA Eraser (Takara, China). Then ...
-
bioRxiv - Microbiology 2020Quote: ... Removal of genomic DNA and synthesis of cDNA were performed using PrimeScript RT reagent Kit with gDNA Eraser (Takara, Japan). The concentration of purified RNA was quantified on a Nanodrop ND-1000 spectophotometer (NanoDrop Technologies ...
-
bioRxiv - Immunology 2020Quote: ... RNA treated with DNase and the cDNAs were synthesized with PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa, #RP047A). Samples without reverse transcriptase were also added as control ...
-
bioRxiv - Genomics 2019Quote: ... The 48-μL preparation volume for reverse transcription (RT) contains 1X SMARTer Kit 5X First-Strand Buffer (5X; Clontech, 634936), 2.5-mM SMARTer Kit Dithiothreitol (100 mM ...
-
bioRxiv - Plant Biology 2022Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using the PrimeScript™ RT Master Mix Kit (Takara). SYBR Premix Ex Taq™ kit (Takara ...
-
bioRxiv - Microbiology 2022Quote: ... The cDNA was acquired by using the Prime Script RT reagent kit with genomic DNA (gDNA) eraser (TaKaRa, Beijing, China). The SYBR Premix Ex Taq II kit (TaKaRa ...
-
bioRxiv - Molecular Biology 2022Quote: ... The titer of harvested lentivirus was established using the Lenti-X RT-qPCR Titration Kit (Takara Bio, Cat. no. 631235). A representative calibration plot and LV tittering of pAIO is added as Supplementary data S4.
-
bioRxiv - Physiology 2022Quote: ... cDNA was generated from 200ng of RNA using PrimeScript RT reagent Kit with gDNA Eraser (Takara Bio; Cat. No. RR047A) according to the manufacturer’s directions ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1 μg of total RNA was reverse transcribed into complementary DNA (cDNA) using PrimeScript™ RT Reagent Kit (Takara, RR047B). Amplification of cDNA product was performed using specific primers with the TB Green® Premix Ex Taq™ II (Takara ...
-
bioRxiv - Microbiology 2023Quote: ... Reverse transcription reaction was set up using the cleaned-up RNA (1.6µg) using PrimeScript™ RT reagent Kit (Takara, Japan). The RT reaction was carried out with buffering temperature at 25 ºC for 2 mins ...
-
bioRxiv - Microbiology 2023Quote: Total RNA was extracted from PAMs using the TRIzol reagent and was reverse transcribed using the PrimeScript RT kit (TaKaRa). qPCR was performed using the PowerUp SYBR Green Master Mix on the ABI StepOnePlus system ...
-
bioRxiv - Immunology 2022Quote: ... according to the manufacturer’s instructions and was further reverse-transcribed into cDNA through the PrimeScript™ RT Reagent Kit with gDNA Eraser (TaKaRa). A LightCycler 480 II (Roche Applied Science ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... PrimeScript RT reagent kit with gDNA Eraser and TB Green® Premix Ex TaqTM were purchased from Takara (Otsu, Japan). Molecular Probes™ Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry ...
-
bioRxiv - Microbiology 2023Quote: ... This (DNA-free) RNA was reverse transcribed into cDNA by using PrimeScript RT reagent Kit with gDNA Eraser (Takara, Japan). Quantitative PCR was performed using a LightCycler 480 (Roche ...
-
bioRxiv - Genetics 2023Quote: ... First-strand cDNA was synthesized using 1.5 µg RNA and Prime Script™ RT reagent Kit with gDNA eraser (Takara) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2024Quote: ... quantitative real-time PCR (RT-qPCR) was performed using the cDNA libraries as the template and a TB Green Premix Ex Taq kit (Takara) in a CFX96 real-time PCR machine (BioRad) ...
-
bioRxiv - Genomics 2022Quote: The genomic DNA of Mir-0 accession (ID 8337) was used as a template (2 ng) for amplification using PrimeSTAR® GXL DNA Polymerase (TaKaRa), in a 40-µl PCR reaction with 0.3 µM primers OP009 and OP010 ...
-
bioRxiv - Developmental Biology 2024Quote: ... 2 µl of cDNA was used together with specific forward and reverse primers for primary miR-43093 (2 µM; Supp. Table 1) and 5 µl of SYBR® Premix-Ex-Taq (Tli RNase H Plus, Takara) in a 10 µl reaction ...
-
bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized using a combination of SMARTer PCR cDNA Synthesis Kit (Takara) and SuperScript II Reverse Transcriptase (ThermoFisher) ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... The cDNA was synthesized using SMARTer® PCR cDNA Synthesis Kit (Clontech, USA) which was then amplified using the KAPA HiFi PCR Kits (Roche ...
-
bioRxiv - Genomics 2020Quote: ... cDNA was synthesized using the SMART PCR cDNA Synthesis Kit (Clontech, CA, USA), and then fractionized with BluePippin® (Sage Science ...
-
bioRxiv - Genetics 2019Quote: ... the PCR fragment was cloned into a vector using an Infusion kit (Takara), and the linear construct was amplified with T7/T3 primer set from the plasmid template ...
-
bioRxiv - Genomics 2021Quote: LR-PCR amplification reaction was performed using PrimeSTAR GXL DNA Polymerase kit (Takara) according to manufacturer”s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... PCR reactions were performed using TaKaRa LA Taq Hot Start polymerase kit (TaKaRa) and 50ng of total genomic DNA in a 50uL PCR reaction ...
-
bioRxiv - Cancer Biology 2022Quote: ... and gel extracted using the NucleoSpin Gel and PCR Clean-up kit (Takara Bio USA Inc ...
-
bioRxiv - Developmental Biology 2022Quote: ... Quantitative PCR was performed using a TB Green Premix DimerEraser Kit (RR091A; Takara). The primer sequences are listed in Supplementary Table S1.
-
bioRxiv - Molecular Biology 2019Quote: LncRNA expression was measured using the SYBR® Green PCR Kit (Takara, Japan) in a blinded fashion ...
-
bioRxiv - Microbiology 2021Quote: ... cDNA molecules were amplified using the Advantage 2 PCR kit (639206, Takara Bio) with initial denaturation at 95 °C for 1 min ...
-
bioRxiv - Cancer Biology 2022Quote: ... DNA was purified using the NucleoSpin Gel & PCR Clean-up Kit (Takara, 740986.20) and 100 bp paired end reads sequenced using Illumina NovaSeq at the Integrated Genomics Operation at MSKCC ...
-
bioRxiv - Neuroscience 2019Quote: ... Physical viral titer was determined using Lenti-X qRT-PCR Titration Kit (Takara), and only viruses greater than 1×1011 GC/ml were used ...
-
bioRxiv - Immunology 2020Quote: ... Total RNA was reverse-transcribed using mRNA Selective PCR kit (TaKaRa Bio-Clontech). Mouse integrin beta 1 ...