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Citations for Takara Bio :
1401 - 1450 of 6767 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... or the PrimeScript RT Master Mix (Takara). In each individual experiment ...
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bioRxiv - Plant Biology 2024Quote: ... using the PrimeScript RT Master Mix (Takara). We then amplified cDNA in Power SYBR (Thermo ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR donor amplicons were purified using the Nucleospin Gel and PCR Clean-up kit (Machery-Nagel, distributed by Takara Bio USA). PCR products were not gel purified ...
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bioRxiv - Bioengineering 2021Quote: ... Viral RNA from pseudovirus infected cells was also quantified by qRT-PCR using the Lenti-X qRT-PCR titration kit (Clontech; 631235) following manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... PCR products to be used as DNA templates were cleaned using a NucleoSpin Gel and PCR Clean-up kit (Takara Bio). In vitro transcription was performed using MAXIscript T7 Transcription Kit (Thermo Fischer Scientific ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... All PCR purifications were performed using the NucleoSpin® Gel and PCR Clean-up kit (TAKARA Bio Inc., San Jose, CA) according to manufacturer protocols ...
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bioRxiv - Microbiology 2024Quote: ... for genotype analysis was extracted using the Qiagen DNeasy Blood and Tissue kit and PCR analysis was done with CloneAmp™ HiFi PCR Premix (Takara) or Phusion polymerase (NEB).
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bioRxiv - Biophysics 2023Quote: ... 5’-CTGGAGAATCCCGGTGC-3’ and 5’- GTGTCAGATATATACATCCTGT-3’ and the PCR product was purified using a NucleoSpin Gel and PCR Clean-up Maxi kit (Takara Bio). The sequences of the 147 bp and 177 bp DNA are as follows:
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bioRxiv - Cell Biology 2021Quote: Cell lysis was assessed by measuring Lactate Dehydrogenase (LDH) activity in cell supernatants using the LDH cytotoxicity detection kit (TaKaRa). To obtain the positive control ...
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bioRxiv - Immunology 2022Quote: Cell death was measured by quantification of the lactate dehydrogenase (LDH) release into the cell supernatant using LDH Cytotoxicity Detection Kit (Takara). Briefly ...
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bioRxiv - Bioengineering 2022Quote: The cells were confirmed to be mycoplasma-negative every few months using a CycleavePCR Mycoplasma Detection Kit (CY232, Takara Bio).
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bioRxiv - Immunology 2021Quote: ... Cell lysis was quantified by measuring the amount of intracellular LDH release into the cell culture supernatant (TaKaRa LDH cytotoxicity detection kit; Clontech). Percentage PI uptake and LDH release were calculated relative to 100 % cell lysis in untreated control sample.
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bioRxiv - Microbiology 2022Quote: ... and E484Q mutations of the SARS-CoV-2 spike protein were determined by qPCR using mutation detection kits purchased from Takara Bio (Shiga ...
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bioRxiv - Molecular Biology 2022Quote: ... Detection relied on a 32P-labeled DNA probe specific for GFP sequence synthesized with a random priming labeling kit (Takara) followed by exposure to film and development as described (43).
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bioRxiv - Molecular Biology 2022Quote: About 1μg of RNAs were reverse transcribed into cDNAs using a PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Japan) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized from the RNA using the PrimeScriptTM RT Reagent Kit with gDNA Eraser (Takara Bio Inc, Shiga, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... Isolated RNA was reverse transcribed into cDNA was by Prime Script TM RT reagent Kit with gDNA Eraser (Stratagene, Takara). Real-time PCR was performed on ABI-7500 real-time PCR machine (Applied Biosystems ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA was synthesized from 1.0 μg of each RNA sample using PrimeScript RT Kit with gDNA Eraser (TaKaRa, Dalian, China).
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bioRxiv - Developmental Biology 2021Quote: ... Reverse transcription was performed using 2.5 µl of the RT MasterMix (SMART-Seq v5 Ultra Low Input RNA Kit for Sequencing, Takara Bio). cDNA was amplified using 8 µl of the PCR MasterMix (SMART-Seq v5 Ultra Low Input RNA Kit for Sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... were reverse transcribed to complementary DNA (cDNA) using both oligo dT and random primers with PrimeScript RT Reagent Kit (Takara) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNAs of 0.5 to 1 μg were used as templates for the reverse transcription using PrimeScriptTM RT Reagent Kit with gDNA Eraser (Takara). Quantitative PCR (qPCR ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg of total RNA from each condition was converted into cDNA using PrimeScript RT reagent with gDNA Eraser kit (Takara Clontech) and MJ Research PTC-220 Dyad PCR System (Conquer Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... First-strand cDNA was synthesized from the total RNA using a PrimeScript™ RT Reagent Kit with gDNA Eraser (Takara) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... Then RNA was reverse-transcribed and amplified using One Step PrimeScriptTM III RT-qPCR Mix kit (Takara Bio, Kyoto, Japan). Primers and probes ...
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bioRxiv - Cell Biology 2022Quote: ... For each specimen 500 ng of total RNA was reversed transcribed into cDNA using PrimeScript RT reagent kit (TaKaRa RR037a) and oligo dT primers according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... the gel-purified RNA was reverse transcribed into cDNA using a PrimeScript RT Reagent Kit with random primers (TAKARA, RR037B), followed by PCR with primers that can amplify transcripts across the splice junction ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was extracted from the isolated tissue and was subjected to reverse transcription (RT) with the use of a NucleoSpin RNA extraction kit (U955C, Takara) and PrimeScript RT reagent kit (RR037 ...
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bioRxiv - Plant Biology 2021Quote: 500 ng of the extracted total RNA were subjected to the first-strand cDNA synthesis with the primescript RT reagent kit (Takara). Quantitative real-time PCR was performed with Thunderbird SYBR qPCR mix (Toyobo ...
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bioRxiv - Developmental Biology 2022Quote: ... was used to purify RNA and 500 ng RNA was used for reverse transcription with a Prime Script RT Reagent Kit (RR037A; Takara). Quantitative PCR was performed using a TB Green Premix DimerEraser Kit (RR091A ...
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bioRxiv - Genomics 2019Quote: ... which was further reverse transcribed into cDNA using a PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa, Tokyo, Japan), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... and then was used for generating the first strand complementary DNA (cDNA) as described in the protocol of the Takara PrimeScript RT reagent Kit with gDNA Eraser (Takara). Briefly ...
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bioRxiv - Cell Biology 2019Quote: ... First-strand cDNA was synthesized from l μg of RNA using the PrimeScript RT Reagent Kit with gDNA Eraser (catalogue No. RR047A, TaKaRa). qPCR was performed in triplicate in 20-μl reactions containing SYBR Premix Ex Taq II (catalogue No ...
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bioRxiv - Microbiology 2021Quote: ... extracted RNA was reverse-transcribed into cDNA using the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa, Dalian, China). The gene quantitative PCR was performed using TB green Premix EX Taq (Takara ...
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bioRxiv - Plant Biology 2021Quote: ... was added to mitigate DNA contamination prior to first-strand cDNA synthesis with PrimeScript™ RT reagent kit (Takara Bio) from approximately 1 g total RNA ...
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bioRxiv - Molecular Biology 2021Quote: ... Two-point five microliters of the polyadenylated sample was used for cDNA synthesis by the PrimeScript RT reagent kit with gDNA eraser (TaKaRa), with the y625 primer in Table S1 ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 1,000 ng of RNA was reverse transcribed using the PrimeScript RT Reagent Kit with gDNA Eraser (TaKaRa, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: ... Single stranded cDNA was synthesized with the oligo(dT) primer using PrimeScript™ RT reagent Kit with gDNA Eraser (Takara), the obtained cDNAs were analyzed by real-time PCR ...
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA was reverse transcribed to complementary DNA (cDNA) using a Prime Script™ RT Master Mix Kit (TaKaRa, China) and the following procedure ...
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bioRxiv - Neuroscience 2020Quote: ... the RNA sample was diluted into 140 ng/μL and purified with the Primescript TM RT regent kit (Takara, RR047). cDNA was generated using SYBR® Premix Ex Taq™ II kit (Takara ...
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bioRxiv - Pathology 2021Quote: ... First-strand complementary DNA was synthesized from total RNA using a PrimeScriptTM RT reagent kit with gDNA Eraser (Takara, China). Then ...
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bioRxiv - Microbiology 2020Quote: ... Removal of genomic DNA and synthesis of cDNA were performed using PrimeScript RT reagent Kit with gDNA Eraser (Takara, Japan). The concentration of purified RNA was quantified on a Nanodrop ND-1000 spectophotometer (NanoDrop Technologies ...
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bioRxiv - Immunology 2020Quote: ... RNA treated with DNase and the cDNAs were synthesized with PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa, #RP047A). Samples without reverse transcriptase were also added as control ...
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bioRxiv - Genomics 2019Quote: ... The 48-μL preparation volume for reverse transcription (RT) contains 1X SMARTer Kit 5X First-Strand Buffer (5X; Clontech, 634936), 2.5-mM SMARTer Kit Dithiothreitol (100 mM ...
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bioRxiv - Plant Biology 2022Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using the PrimeScript™ RT Master Mix Kit (Takara). SYBR Premix Ex Taq™ kit (Takara ...
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bioRxiv - Microbiology 2022Quote: ... The cDNA was acquired by using the Prime Script RT reagent kit with genomic DNA (gDNA) eraser (TaKaRa, Beijing, China). The SYBR Premix Ex Taq II kit (TaKaRa ...
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bioRxiv - Molecular Biology 2022Quote: ... The titer of harvested lentivirus was established using the Lenti-X RT-qPCR Titration Kit (Takara Bio, Cat. no. 631235). A representative calibration plot and LV tittering of pAIO is added as Supplementary data S4.
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bioRxiv - Physiology 2022Quote: ... cDNA was generated from 200ng of RNA using PrimeScript RT reagent Kit with gDNA Eraser (Takara Bio; Cat. No. RR047A) according to the manufacturer’s directions ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription reaction was set up using the cleaned-up RNA (1.6µg) using PrimeScript™ RT reagent Kit (Takara, Japan). The RT reaction was carried out with buffering temperature at 25 ºC for 2 mins ...
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bioRxiv - Microbiology 2023Quote: Total RNA was extracted from PAMs using the TRIzol reagent and was reverse transcribed using the PrimeScript RT kit (TaKaRa). qPCR was performed using the PowerUp SYBR Green Master Mix on the ABI StepOnePlus system ...
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bioRxiv - Immunology 2022Quote: ... according to the manufacturer’s instructions and was further reverse-transcribed into cDNA through the PrimeScript™ RT Reagent Kit with gDNA Eraser (TaKaRa). A LightCycler 480 II (Roche Applied Science ...