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Citations for Takara Bio :
1701 - 1750 of 7163 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Site-specific mutagenesis was achieved using PCR with a Prime STAR Mutagenesis Basal Kit (Takara).
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bioRxiv - Genomics 2021Quote: ... cDNA was reverse transcribed using the SMRTer PCR cDNA synthesis kit (Clontech, Mountain View, CA) from 329 ng and 374 ng of total RNA for brain and testis respectively ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was generated according to package instructions for SMARTer PCR cDNA synthesis kit (Clontech/TakaraBio).
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bioRxiv - Immunology 2022Quote: ... The reactions were pooled and purified with the Nucleospin gel and PCR purification kit (Takara). The purified PCR product was analyzed 2% agarose gel and subjected to sequencing on a HiSeq4000 (Fulgent Genetics) ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA libraries were then synthesized using the SMARTer PCR cDNA Synthesis Kit (Clontech; PT4097-1) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 2×SYBR Green PCR Mastermix (RR820A) and reverse transcription kit (RR047A) were purchased from TAKARA company ...
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bioRxiv - Neuroscience 2023Quote: ... and the resulting titers estimated using the Lenti-X qRT-PCR Titration Kit (Takara Bio), all following manufacturers’ recommendations ...
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bioRxiv - Developmental Biology 2023Quote: ... Genotyping was done using the Terra™ PCR Direct kit (Takara Bio, Kusatsu, Shiga Japan). Embryonic sex and Lhx2 allele genotyping were performed as mentioned [26] ...
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bioRxiv - Genetics 2019Quote: First strand cDNA was synthesized from 1 μg total RNA by using a PrimeScript RT Reagent Kit with gDNA Eraser (Takara Bio, Kusatsu, Japan). qRT-PCR was performed in a 25 μl reaction volume with SYBR Premix Ex Taq II Tli RNaseH Plus (Takara Bio ...
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bioRxiv - Molecular Biology 2021Quote: ... One µg of total RNAs was subjected to reverse transcription into cDNAs using PrimeScriptTM RT reagent Kit with gDNA Eraser (Takara, cat. no. RR047A) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... and the total RNA was reverse transcribed into cDNA using the PrimeScript RT Reagent Kit with gDNA Eraser (TaKaRa, RR047A, Kusatsu, Shiga, Japan). The diluted cDNA was used as a template for qPCR ...
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bioRxiv - Cancer Biology 2019Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa, Dalian, China). SYBR Premix Ex Taq II (TaKaRa ...
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bioRxiv - Genetics 2020Quote: ... RNA was extracted from samples using TRIzol and cDNA synthesized from 1 μg RNA template using PrimeScript™ RT reagent Kit with gDNA Eraser (TAKARA BIO INC). The sequences of primers used for amplification are shown in Table S6 ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA (1 μg) was reverse transcribed to cDNA using the PrimeScriptTM RT reagent kit with gDNA Eraser (Cat#: RR047A, TaKaRa Bio, Shiga, Japan). qRT–PCR was performed using a LightCycler 480 II instrument (Roche Molecular Systems ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was reverse transcribed from 500 ng of total RNA with hexamers using a PrimeScript RT reagent kit (Takara Bio, Inc., Kusatsu, Japan). qPCR was then performed in a 25 μl reaction using TB Green Premix Ex Taq II reagent (Tli RNaseH Plus ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The cDNA was synthesized using 1 µg of RNA from each treatment using the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa Dalian, China). RT-qPCR was performed on a Bio-Rad CFX96 (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Approximately 1 µg of total RNA was used for reverse transcription using the PrimeScript™ RT reagent kit with gDNA Eraser (Takara, Beijing, China). After the transcribed cDNAs were 5-fold diluted ...
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bioRxiv - Immunology 2022Quote: RNA was extracted from LNs and used for RT-qPCR using a Prime Script™ II Strand cDNA Synthesis kit (Takara, Dalian, China), in accordance with the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Equal amounts of total RNA were used to synthesize cDNA with the PrimeScript™ RT reagent Kit with gDNA Eraser (Takara RR047A, China). Quantitative real-time reverse-transcription PCR (qRT–PCR ...
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bioRxiv - Neuroscience 2023Quote: ... total RNA was isolated by standard procedure from 20-30 heads of homozygous Aprt5 flies and reverse transcribed using oligo d(T) primers (PrimeScript RT Reagent Kit, Takara Bio, Kyoto, Japan). At least 750 ng of the first strand cDNA was amplified by PCR in 20 μl of reaction mixture using PrimeStar Max DNA polymerase (Takara Bio ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal quantities (5 ng) of total RNA from each sample were used for cDNA synthesis using the PrimeScript®RT reagent kit (TaKaRa, Tokyo, Japan). The reverse transcriptions of miRNAs were performed using looped miRNA-specific RT primers for miRNAs ...
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bioRxiv - Cancer Biology 2023Quote: ... 1μg of isolated RNA was taken from each sample was then transcribed to cDNA using PrimeScript ™ RT reagent kit with gDNA Eraser (Takara Bio RR047B). According to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The first-strand cDNAs of mRNA and mature miRNA were synthesized from 500 ng of total RNA using PrimeScript™ RT reagent kit with gDNA Eraser (Cat# RR047A, Takara, Kyoto, Japan) and miRcute Plus miRNA First-Strand cDNA Synthesis Kit (Cat# KR211 ...
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bioRxiv - Cancer Biology 2023Quote: ... obtained by reverse-transcribing the total RNA extracted from HCT116 cells using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara Bio, Dalian, China) was used as the template for amplifying the corresponding regions using the PrimeSTAR Max DNA Polymerase (Takara Bio) ...
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bioRxiv - Genetics 2024Quote: 1 µg of gDNA Eraser-treated RNA was reverse transcribed into cDNA using the PrimeScrip® RT reagent Kit with gDNA Eraser (Takara Bio, Japan), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA was reverse transcribed from 1 μg of total RNA using the PrimeScriptTM RT reagent kit (AK4201; Takara Bio, Kusatsu, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The SARS-CoV-2 viral load was quantified in culture supernatant using RT-QPCR with primer probes targeting E gene of SARS-CoV-2 using PrimeDirect Probe RT-qPCR Mix (TaKaRa Bio USA, Inc) and Applied Biosystems QuantStudio3 real-time PCR system (Applied Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: Whole Genome Amplification (WGA) was performed on 300 single cells for each Time by means of SMARTer PicoPLEX Single Cell WGA kit (Takara) following manufacturer’s protocol as previously described24 ...
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bioRxiv - Plant Biology 2021Quote: ... after-ripened Col and ref6c seeds collected at different imbibition time points were subjected for total RNA extraction using Minibest plant RNA extraction kit (Takara). Three independent biological replicates were performed ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR (CloneAmp HiFi PCR Premix, Takara-Bio Europe) genotyping was done with primers designed for each knock-out line that allowed for confirming either the presence of the wild-type (F-gt5/R-gt5WT and F-gt3WT/R-gt3 ...
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bioRxiv - Plant Biology 2023Quote: ... we PCR amplified (CloneAMP HIFI PCR premix, TAKARA) the coding sequences of ATG8a ...
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bioRxiv - Plant Biology 2019Quote: ... A first-strand cDNA was obtained from 1 μg of total RNA using a PrimeScript® RT reagent kit (Takara Bio, Inc., Otsu, Japan). The sequences of candidate genes were obtained from the local alkaligrass EST database using a BLASTn program ...
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bioRxiv - Cell Biology 2019Quote: ... The cDNA was synthesized by reverse transcription using 1 μg of total RNA as a template with a PrimeScript™ RT Reagent Kit with gDNA Eraser (Takara, Beijing, China, RR047A). The SYBR® Premix Ex Taq TM II kit (Takara ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated from ten individual mites using Trizol® reagent and was then treated with a PrimeScript™ RT synthesis kit with genomic gDNA eraser according to instructions (TaKaRa, Beijing, China). A 414 base pair (bp ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 1 µg of RNA was reverse transcribed to complementary DNA using the PrimeScript™ RT reagent Kit (Takara Bio, Otsu, Japan), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5 µg of quality checked total RNA was converted into first-strand cDNA using a PrimeScript RT Reagent Kit with gDNA Eraser (TaKaRa Bio Inc, Shiga, Japan), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA from the same amount of RNA between RNAi and control samples were synthesized in a 20 μl reaction volume by the PrimeScript™ RT reagent Kit with gDNA Eraser (TAKARA, Code No. RR047A). One μl of cDNA was used as the template in a 10 μl reaction volume of the PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Developmental Biology 2019Quote: ... cDNA was synthesized using PrimeScript™ RT Master Mix (Takara) from 500 ng total RNA and diluted 1:10 in RNase-free water for OCT3/4 and NANOG mRNA expression analysis ...
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bioRxiv - Immunology 2021Quote: ... and SMARTScribe RT (Takara Bio/Clontech, #639536, 100 U/ul) with the denatured RNA and incubating for 90 min at 42°C then heating to 70°C for 10 min ...
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bioRxiv - Immunology 2021Quote: ... and SMARTScribe RT (Takara Bio/Clontech, #639536, 100 U/ul) with the denatured RNA and incubating for 90 min at 42°C then heating to 70°C for 10 min ...
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bioRxiv - Plant Biology 2020Quote: ... The Takara Prime ScriptTM RT Master Mix (Takara Bio Inc.) was used for cDNA synthesis.
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using SYBR Premix Ex Taq (Takara). Roche LightCycler 480 thermal cycler was used to assess the melting curve and quantification of the PCR products ...
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bioRxiv - Microbiology 2020Quote: ... RNA was reversely transcribed into cDNA (SmartScribe RT, Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and first strand cDNA prepared using the PrimeScript RT (Clontech) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... First strand reverse transcription (RT) using Smartscribe Reverse Transcriptase (Clontech) and SmartSeq template switching oligo (TSO ...
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bioRxiv - Molecular Biology 2024Quote: ... After 2 hrs 1µl of Reverse Transcriptase (PrimeScript RT, Takara) was added and reverse transcription was performed at 420C for 1hr followed by inactivation at 700C for 30 mins ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized using the PrimeScript RT Master Mix (Takara).
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bioRxiv - Pathology 2024Quote: ... and PrimeScript™ RT Master Mix (Takara Bio, Kusatsu, Japan) were used to synthesize cDNA and perform RT-qPCR ...
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bioRxiv - Microbiology 2020Quote: ... The synthesized cDNA was purified using a NucleoSpin Gel and PCR Clean-up Kit (Takara Bio) and eluted with 30 μl of 5 mM Tris-HCl buffer (pH 8.5) ...
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bioRxiv - Molecular Biology 2021Quote: ... The digested PCR product was cloned into a TA-vector by Mighty TA-cloning Kit (Takara). After LacZ selection ...