Labshake search
Citations for Takara Bio :
1651 - 1700 of 7163 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Ad5 stocks were tittered by detection of the Ad hexon protein using Adeno-X™ Rapid Titer Kit (Cat. No. 632250, Takara, Clontech). The Ad5 CPE was dose-dependent ...
-
bioRxiv - Molecular Biology 2020Quote: ... An amount of 400 ng of the extracted RNA was subjected to reverse transcription by using a PrimeScript RT Reagent Kit (Takara, Shiga, Japan). With complementary DNA (cDNA ...
-
bioRxiv - Molecular Biology 2021Quote: ... Purified viral RNA (vRNA) was reverse transcribed into cDNA using PrimeScript RT reagent Kit with gDNA Eraser (Takara Bio Inc., Shiga, Japan). The number of RV genome copy in the medium supernatant was determined using RT-qPCR with general or serotype-specific Taqman probe and primers (Additional file 2 ...
-
bioRxiv - Genomics 2019Quote: The first-strand cDNA was synthetised from 1 μg of 5 tissues (root, stem, leaf, flower and peel) total RNA using Prime Script RT Reagent Kit (Takara, Dalian, China). The qRT-PCR reactions were performedin 96-well plates using the ABI 7500 fast Real-Time PCR system (Applied Biosystems ...
-
bioRxiv - Molecular Biology 2019Quote: ... The same samples used for RNA sequencing were used for reverse transcription and synthesis of cDNA with PrimeScript RT Reagent Kit with gDNA Eraser following the instructions of the manufacturer (TaKaRa, Dalian, China). For designing the primers for qRT-PCR ...
-
bioRxiv - Plant Biology 2019Quote: ... 1μg purified total RNA was used to obtain the first-strand cDNA with the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa, Japan). The Takara SYBR ® Premix Ex Taq™ II was used to perform the qRT-PCR assay using primers P13 and P14 ...
-
bioRxiv - Molecular Biology 2019Quote: ... Total RNA (1 µg) was reverse transcribed into First-strand complementary DNA using a PrimeScript RT reagent kit with gDNA Eraser (Takara, Dalian, China) according to the manufacturer’s instructions and stored at –20°C.
-
bioRxiv - Plant Biology 2019Quote: ... Two micrograms of total RNA and 200 ng of a random primer were used in reverse transcription (RT) through the cDNA synthesis kit (TaKaRa, Dalian, China) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... The WNV replicon RNAs were isolated from the pellet and reverse-transcribed using Prime Script™ RT reagent Kit with gDNA Eraser (Takara, Japan). Quantitative PCRs were performed using a SYBR PremixEX Taq II (RT)-PCR kit (Takara ...
-
The pan-cancer lncRNA PLANE regulates an alternative splicing program to promote cancer pathogenesisbioRxiv - Cancer Biology 2020Quote: ... cDNA was synthesized from 1 μg of total RNA using the PrimScriptTM RT reagent Kit with gDNA Eraser (TaKaRa, #RR047A; Dalian, China). Of the resultant cDNA ...
-
bioRxiv - Microbiology 2022Quote: ... Total RNA (500 ng) from the worms was reverse transcribed into cDNA by using a PrimeScript RT Reagent Kit (TaKaRa, Dalian, China) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2022Quote: 500 ng of total RNA extracted from cell culture were subjected to reverse transcription, using ISOGEN II (NIPPON GENE, Tokyo, Japan) and PrimeScript RT reagent Kit (TaKaRa, Kusatsu, Japan). The resulting cDNA was subjected to real-time PCR analysis using glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
-
bioRxiv - Plant Biology 2020Quote: ... and one microgram of the DNase-treated RNA was used for cDNA synthesis using PrimeScript RT reagent kit (Takara Bio, CA, USA). The expression analysis was performed using TB green Premix Ex Taq II (Takara Bio ...
-
bioRxiv - Immunology 2022Quote: ... cDNA was synthesized to a total of 500ng RNA in a 20 μL system using PrimeScript™ RT Reagent kit (TAKARA, #RR036A). q-PCR were performed on anlytik jena (qTOWER 2.0 ...
-
bioRxiv - Microbiology 2019Quote: ... A pair of primers that match the sr8384 sequence were separately added into the total RNA to synthesize the first-strand cDNA by using the PrimeScript RT Reagent Kit (TaKaRa, cat. #RR047A). Next ...
-
bioRxiv - Pathology 2021Quote: ... The number of SARS-COV-2 copies were quantified using Direct One-Step RT-qPCR Mix for SARS-CoV-2 kit (Takara Bio Inc.).
-
bioRxiv - Cancer Biology 2020Quote: ... Purified RNA samples were reverse-transcribed into cDNA using the PrimeScript™ RT Reagent Kit with gDNA Eraser (RR047A, TaKaRa, Tokyo, Japan).
-
bioRxiv - Microbiology 2019Quote: ... and the first strand of cDNA synthesis was performed using the PrimeScript™ RT reagent Kit with gDNA Eraser (Takara, Dalian, China). All quantitative PCR were performed in triplicate on an ABI 7500 Fast real-time PCR system (Applied Biosystems ...
-
bioRxiv - Immunology 2020Quote: ... the reverse transcription (RT) reaction was performed in a two-step method following the PrimeScript™ II Reverse Transcriptase kit protocol (Takara, Japan). For the first step ...
-
bioRxiv - Physiology 2023Quote: ... cDNA was made from 100-400 ng of DNase-treated total RNA using a Takara PrimeScript RT Reagent Kit with gDNA Eraser (Takara bio RR047B). Quantitative PCR was performed using TB Green™ Premix Ex Taq™ (Tli RNaseH Plus ...
-
bioRxiv - Synthetic Biology 2022Quote: ... and inputted into cDNA synthesis by incubation with a mixture of random hexamers and reverse transcriptase (TAKARA PrimeScript RT Reagent Kit with gDNA Eraser, Takara Bio #RR047A). The resulting cDNA was diluted 1:10 and 2 μl of each sample was amplified using a QuantStudio 5 (Applied Biosystems ...
-
bioRxiv - Plant Biology 2024Quote: ... A total of 2 ug of the total RNA from each sample were used to synthesize the cDNA using PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Dalian, China) according to the manufacturer’s instructions ...
-
bioRxiv - Pathology 2024Quote: First strand cDNA was generated from 1 μg of total RNA using the Prime Script RT Reagent Kit with gDNA Eraser (TaKaRa, Dalian, China). Real-time PCR was performed by using an SYBR Green PCR Master Mix (TaKaRa ...
-
bioRxiv - Genomics 2024Quote: ... and one microgram of the DNase-treated RNA was used for cDNA synthesis using PrimeScript RT reagent kit (Takara Bio, CA, USA). The expression analysis was performed using TB green Premix Ex Taq II (Takara Bio ...
-
bioRxiv - Biochemistry 2023Quote: ... 1 μg of total RNA was used to make cDNA libraries using prime script RT reagent kit gDNA eraser (Takara Bio Inc.) according to the kit’s protocol ...
-
bioRxiv - Immunology 2023Quote: ... 110 ng of RNA were retrotranscribed in a final volume of 10 μL using the PrimeScript RT reagent Kit (Takara, Kusatsu, Japan) with a combination of oligo-d(T ...
-
bioRxiv - Physiology 2023Quote: ... The cDNA was synthesized from 400 ng of DNase-treated total RNA by the Takara PrimeScript RT Reagent Kit with gDNA Eraser (Takara bio RR047B). Quantitative PCR was performed using TB Green™ Premix Ex Taq™ (Tli RNaseH Plus ...
-
bioRxiv - Cell Biology 2023Quote: ... MA) and PrimeScript RT reagent kit with gDNA Eraser and SYBR Premix Ex Taq II (Tli RNase H Plus) (TaKaRa, Kusatsu, Japan) (Taniguchi and Yoshida ...
-
bioRxiv - Immunology 2021Quote: ... the RT-RamDA mixture containing 2.5x PrimeScript Buffer (TAKARA), 0.6 μM oligo(dT)18 (Thermo) ...
-
Cecropins contribute to Drosophila host defence against fungal and Gram-negative bacterial infectionbioRxiv - Immunology 2021Quote: ... Reverse transcription was carried out using PrimeScript RT (TAKARA) with random hexamers and oligo dTs ...
-
bioRxiv - Neuroscience 2020Quote: ... containing 2 μL of 5X SMARTScribe RT buffer (Takara), 0.5 μL of 100 mM DTT (Millipore Sigma) ...
-
bioRxiv - Cell Biology 2022Quote: ... cDNAs were synthesized with PrimeScript RT Master mix (Takara) followed by adding template RNA ...
-
bioRxiv - Physiology 2022Quote: ... After RNA Reverse transcription (Prime Script RT reagent, Takara), cDNA levels were measured by qPCR Fast SYBR™ Green Master Mix (Ref:4385618 ...
-
bioRxiv - Molecular Biology 2019Quote: ... The RT product was amplified using Ex taq (TaKaRa) for variable exons 4 ...
-
bioRxiv - Systems Biology 2021Quote: ... reverse transcription (TaKaRa Bio PrimeScript RT with gDNA eraser) and RT-qPCR (Thermo-Fisher Scientific PowerUp SYBR green ...
-
bioRxiv - Developmental Biology 2021Quote: ... cDNAs were synthesized with PrimeScript RT Master mix (Takara) followed by adding template RNA ...
-
bioRxiv - Molecular Biology 2024Quote: ... After RNA Reverse transcription (Prime Script RT reagent, Takara), cDNA levels were measured by qPCR Fast SYBR™ Green Master Mix (Ref ...
-
bioRxiv - Cancer Biology 2023Quote: ... cDNA was generated with PrimeScript RT Master Mix (Takara). qPCR was performed on StepOnePlus Real-Time PCR System (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2023Quote: ... Reverse transcription was carried out using SmartScribe RT (Takara), and Q5 polymerase (New England Biolabs ...
-
bioRxiv - Neuroscience 2023Quote: ... The Prime Script RT Master Mix (Takara Bio, RR036A) was used to synthesize cDNA according to the manufacturer’s instructions ...
-
bioRxiv - Physiology 2023Quote: ... After RNA Reverse transcription (Prime Script RT reagent, Takara), cDNA levels were measured by qPCR Powerup SYBRTM Green Master Mix (Ref ...
-
bioRxiv - Developmental Biology 2021Quote: TdT-mediated dUTP nick end labeling (TUNEL) staining was carried out with In Situ Apoptosis Detection Kit (MK500, Takara Bio Inc., Shiga, Japan), according to the manufacturer’s instruction ...
-
bioRxiv - Developmental Biology 2021Quote: TdT-mediated dUTP nick end labeling (TUNEL) staining was carried out with In Situ Apoptosis Detection Kit (MK500, Takara Bio Inc., Shiga, Japan), according to the manufacturer’s instruction ...
-
bioRxiv - Genomics 2020Quote: Copy DNAs were generated from polyA(+) RNA fractions using SMARTer PCR cDNA Synthesis Kits (Clontech) according to PacBio Isoform Sequencing (Iso-Seq ...
-
bioRxiv - Cell Biology 2019Quote: ... followed by PCR-mediated amplification and plasmid insertion with the in-Fusion cloning kit (Clontech). The CLN6ΔL2 construct was obtained by removing the codons for amino acids 155-222 using the Q5 Site-Directed Mutagenesis kit (New England Biolabs).
-
bioRxiv - Genetics 2019Quote: ... The lentiviral titers were determined with a Lenti-X™ qRT-PCR Titration Kit (Clontech).
-
bioRxiv - Plant Biology 2020Quote: Generation of expression constructs was performed with both In-Fusion Advantage PCR Cloning Kit (Clontech) and Gateway Cloning Technology (Invitrogen).
-
bioRxiv - Cancer Biology 2022Quote: ... qPCR was performed to detect mRNA expression of genes by TB Green PCR Kit (TaKaRa) and primers are shown in table S2.
-
bioRxiv - Cell Biology 2022Quote: ... PCR reactions were carried out using Hot Start TaKaRa LA Taq kit (Takara Biotechnology, #RR042A) with the following cycling conditions ...
-
bioRxiv - Pathology 2021Quote: ... Viral RNA genome content was quantified using the Lenti-X qRT-PCR Titration Kit (Takara) and the Quant-X One-Step qRT-PCR TB Green Kit (Takara) ...