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Citations for Takara Bio :
1301 - 1350 of 6767 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... The RNA was reverse-transcribed into cDNA by using a Prime ScriptTM RT Reagent Kit (TaKaRa, Kyoto, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... One µg of total RNA was processed using PrimeScript RT reagent Kit with gDNA Eraser (Takara, Beijing, China). Amplified products were sequenced and verified after cloning into a pJET1.2-T vector through CloneJET PCR Cloning Kit (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription was performed to make cDNA using PrimeScript RT reagent kit with gDNA Eraser (TaKaRa, Kyoto, Japan). Sequences encoding the C-terminal portion (amino acid 45 to 131 ...
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bioRxiv - Biophysics 2023Quote: ... The first-strand cDNA was reverse transcribed with PrimeScript™ RT Reagent Kit with gDNA Eraser (TaKaRa, Japan). Quantitative real-time PCR was run on a Roche LightCycler® 480 II real-time PCR detection system ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting samples were reverse transcribed into first-strand cDNA using a PrimeScript RT Reagent Kit (Takara Bio). Quantitative PCR (qPCR ...
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bioRxiv - Zoology 2024Quote: ... One µg of total RNA was processed using PrimeScript RT reagent Kit with gDNA Eraser (Takara, Beijing, China). The PCR procedure was set as follows ...
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MALAT1 mediates miR-206/CDC42/PAK1/Paxillin signaling axis to alleviate erectile dysfunction in ratsbioRxiv - Molecular Biology 2024Quote: ... And the obtained RNA was used for reverse transcription using the PrimeScript RT Enzyme Mix I kit (TaKaRa). After the reaction was completed ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated with Trizol (Thermo) and cDNA was synthesized with Prime Script RT Reagent Kit (Takara). Quantitative PCR was performed with a SYBR Green PCR kit (Toyobo ...
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bioRxiv - Cell Biology 2023Quote: cDNA was synthesized from the total RNA of HapT1 cells using the PrimeScript RT Reagent Kit (Takara Bio). The amounts of Pts and Actb were quantified on a LightCycler 480 (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... PCR products were inserted using the In-Fusion cloning kit (Clontech) into the pBMDEL that had been linearized with BfuAI ...
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bioRxiv - Microbiology 2020Quote: ... PCR was conducted using the PrimeSTAR GXL kit (Takara, Cat. # R050B) with ThermaStop™ (Thermagenix ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was generated by using SMARTer PCR cDNA Synthesis Kit (Clontech). Gene-specific primers were designed for each gene of interest (Supp ...
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bioRxiv - Microbiology 2022Quote: ... Genotyping was performed using Terra PCR Direct Genotyping Kit (Takara, 639285) following protocol modified from The Jackson Laboratory ...
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bioRxiv - Molecular Biology 2023Quote: ... The reverse transcription and qRT-PCR kits were purchased from Takara; Trizol was purchased from ambion ...
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bioRxiv - Cell Biology 2023Quote: ... gel-purified (NucleoSpin Gel and PCR Clean-up kit from Takara) and the purified fragment was stored at -20°C ...
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bioRxiv - Cell Biology 2023Quote: ... followed by qRT-PCR with SYBR green master mix kit (Takara). The reactions were performed in triplicate using the Agilent Mx3000P qPCR system and analysis was performed essentially as described earlier (Panigrahi et al. ...
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bioRxiv - Genomics 2024Quote: ... Final libraries were purified with Nucleospin PCR Clean up kit (Takara) and quantified by Qubit HS (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... or PrimeScrip RT Master Mix (Takara) (mRNA) ...
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bioRxiv - Immunology 2021Quote: ... For reverse transcription SmartScribe RT (Clontech) and corresponding buffer was used ...
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bioRxiv - Plant Biology 2022Quote: ... PrimeScript RT Master Mix (Takara Bio) was used for cDNA synthesis and quantitative RT-PCR was performed with PowerUp SYBR Green Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... 5× PrimeScript™ RT mix (TaKaRa) was used to acquire cDNA ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The product from this PCR was purified and cleaned with NucleoSpin Gel and PCR clean-up kit from TAKARA. A 2nd PCR was performed with high-fidelity PrimeSTAR GXL DNA Polymerase (Takara Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... and then used as the template to synthesize double strand cDNA amplicons by PCR (optimal 17 – 21 cycles) using Advantage 2 PCR kit according to the manufacturer’s specifications (Clontech). cDNA was purified using NucleoSpin Gel and PCR Clean-up kit (Takara Bio) ...
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bioRxiv - Cancer Biology 2024Quote: ... Barcode sequence of each clone was PCR amplified directly from the cells using Terra PCR Direct Polymerase kit (Takara) for Sanger sequencing using previously described primers (5).
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bioRxiv - Evolutionary Biology 2023Quote: SARS-CoV-2 cDNA was generated from 100 ng of RNA in a RT-PCR reaction buffer containing 4 μL of 5X PrimeScript IV 1st strand cDNA Synthesis Mix (Takara Bio, Kusatsu, Shiga, Japan), 2 μL of 50 μM random hexamer primer ...
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bioRxiv - Biochemistry 2022Quote: ... Total RNA was isolated using RNAqueous isolation kit (Thermo-Fisher, Waltham MA) and mRNA reverse transcribed using PrimeScript RT Reagent Kit (Takara Bio USA, Mountain View CA). Quantitative PCR reactions were carried out using PowerUp SYBR-Green master mix using StepOne Real-Time PCR systems (Applied Biosystems) ...
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bioRxiv - Immunology 2021Quote: Cell death was assessed by the release of LDH in conditioned medium using LDH Cytotoxicity Detection Kit (TaKaRa, CA).
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bioRxiv - Immunology 2021Quote: Cell death was assessed by the release of LDH in conditioned medium using LDH Cytotoxicity Detection Kit (TaKaRa, CA). IL-1β levels in conditioned media were measured by ELISA (eBiosciences ...
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bioRxiv - Cell Biology 2023Quote: Cell death was assessed by the release of LDH in conditioned medium using LDH Cytotoxicity Detection Kit (TaKaRa, CA). IL-1β levels in conditioned media were measured by an ELISA kit (eBiosciences ...
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bioRxiv - Neuroscience 2020Quote: ... Viral titer was determined using a qPCR Lentivirus Titration Kit (Lenti-X, qRT-PCR Titration Kit, Takara). For smaller scale virus preparation ...
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bioRxiv - Bioengineering 2021Quote: The target fragments are amplified by PCR using primers (Sangon Biotech, Shanghai, China) and PCR amplification kit (Takara Bio, Japan). The PCR amplifies the DNA fragments including the 1900 bp target ...
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bioRxiv - Physiology 2021Quote: ... The RNA (1 ug/sample) was reverse transcribed into cDNA and Q-PCR was performed using a SYBR Green PCR kit (Takara) in a Light Cycler (Eppendorf) ...
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bioRxiv - Microbiology 2020Quote: ... The 1.5 kb PCR product was isolated and purified from agarose gel using the NucleoSpin Gel and PCR Clean-up kit (Takara). The product was subjected to Sanger dideoxy sequencing at GENEWIZ (San Diego ...
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bioRxiv - Zoology 2019Quote: ... were obtained from the mRho.V5.mER.hOr47a construct via PCR using the Advantage 2 PCR kit (Cat. Nr. 639206, Takara, Kusatsu, Japan) using the E.hOr47a_fwd and hOr47a_fwd forward primers ...
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bioRxiv - Molecular Biology 2021Quote: ... Homology arms complementary to the pUC19 cloning vector were then added to the PCR product with another PCR in order to clone the RACE products with the In-Fusion HD Cloning kit (Takara). Individual clones were selected and Sanger sequenced.
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bioRxiv - Immunology 2024Quote: ... we ran our linearized plasmid backbone and 83 bp PCR products on a 1% agarose gel and recovered each using the Gel and PCR Clean-up Kit (Takara) per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The first-strand cDNA was synthesized from genomic DNA-removed RNA by using PrimeScript™ RT reagent kit (TaKaRa). Next ...
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bioRxiv - Immunology 2021Quote: ... The first-strand cDNA synthesis was performed with the Prime Script RT Master Mix kit (Takara Bio Europe, France). Quantitative real-time PCR was performed at 60°C using Takyon ROX SYBR MasterMix (Eurogentec ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal volume RNAs were subjected to reverse transcription using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Japan). Distribution of mat mRNAs or mat-exon1 mRNAs in transfected cells was assessed using SBYR-Green (Takara ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized from 500 ng total RNA using a Prime Script RT Reagent Kit (Takara Bio, Otsu, Japan). qRT-PCR was performed using a StepOne Real-time PCR Instrument (Applied Biosystems ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA (30 ng) was used for synthesis of cDNA with PrimerScript RT reagent kit with gDNA eraser (TAKARA BIO). QPCR was performed with TB Green Premix Ex-taq II (TAKARA BIO) ...
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bioRxiv - Plant Biology 2020Quote: ... Approximately 2 μg of total RNA was reverse-transcribed using PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa). Real-time quantitative RT-PCR was performed with TB Green Premix Ex Taq™ (TakaRa ...
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bioRxiv - Plant Biology 2021Quote: ... and first-strand cDNA was synthesized from 500 ng total RNA using the PrimeScript RT reagent Kit (Takara, Japan). Semi-quantitative RT-PCR was performed using PrimeSTAR GXL (Takara ...
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bioRxiv - Microbiology 2020Quote: ... SYBR Green-based RT-qPCR were used according to the manuals by SYBR Premix Ex Taq kit (Takara Bio). Primers used in RT-qPCR reactions as follows ...
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bioRxiv - Cell Biology 2021Quote: ... Total RNA (1 μg) was converted to cDNA using the PrimeScript™ RT reagent Kit with gDNA Eraser (TAKARA). Quantitative PCR (qPCR ...
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bioRxiv - Genetics 2022Quote: ... RNA was reverse transcribed to cDNA using the PrimeScript™ RT reagent Kit with gDNA eraser (Takara, Kusatsu, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Each RNA sample was reverse-transcribed to cDNA after DNase I treatment using a PrimeScript RT reagent kit (Takara). PCR was performed as described in Kumar et al ...
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bioRxiv - Cell Biology 2021Quote: ... An equal amount of RNA for each sample was converted to cDNA using PrimescriptTM RT Reagent Kit (Takara Bio), or Superscript IV (Thermofisher Scientific) ...
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bioRxiv - Genetics 2022Quote: ... 1 µg of total RNA was used for reverse transcription using PrimeScript RT Reagent Kit (Cat#RR037A, TaKaRa, Japan). Quantitative polymerase chain reaction (qPCR ...
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bioRxiv - Biochemistry 2022Quote: One μg of RNA per kidney half was reverse-transcribed using PrimeScript RT Reagent kit (RR037 TAKARA, Shiga, Japan). Two μl of cDNA was used for quantitative real-time PCR to assess gene mRNA expression ...