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Citations for Takara Bio :
1451 - 1500 of 7163 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Reverse transcription of RNA into cDNA was accomplished with the PrimeScript RT reagent Kit with gDNA Eraser (RR047A, Takara), followed by quantitative RT-PCR (qRT–qPCR ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 ug of RNA was reverse-transcribed into cDNAs using the Prime Script RT Reagent Kit (Takara, RR037B) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... and an aliquot of 100 ng was reverse transcribed with the PrimeScript RT Reagent Kit with gDNA Eraser (Takara) and the included primer mix ...
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bioRxiv - Microbiology 2022Quote: ... Isolated RNA or serial ten-fold dilutions of RNA standards for the ORF1ab amplicon (ranging from 2.25×10^6 to 250 copies/rxn) were reverse transcribed using the Takara Prime Script RT kit (Takara) using poly(A ...
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bioRxiv - Biochemistry 2023Quote: ... cDNA was prepared from 1 µg of total RNA using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara). Real-time polymerase chain reaction (real-time PCR ...
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bioRxiv - Genomics 2023Quote: Five µg of each Quartet RNA sample was reverse transcribed using the PrimeScript™ RT reagent Kit (RR037A, TaKaRa) in a 50 µl reaction ...
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bioRxiv - Immunology 2024Quote: ... 500 ng of total RNA was used for synthesis of cDNA using PrimeScriptTM RT reagent Kit (Takara, Tokyo, Japan). For miRNA expression analysis ...
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bioRxiv - Molecular Biology 2024Quote: ... and subjected to RT with the use of a TaKaRa PrimeScript II 1st Stand cDNA Synthesis Kit (Takara Bio). The resulting cDNA was subjected to real-time PCR analysis with Fast SYBR Green Master Mix (3485612 ...
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bioRxiv - Biochemistry 2019Quote: ... All PCR products were inserted using the In-Fusion cloning kit (Clontech) into the pBMDEL which had been linearized with BfuAI ...
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bioRxiv - Developmental Biology 2019Quote: ... the cDNA was amplified with the Advantage 2 PCR Kit (Clontech Takara) containing buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... the cDNA was amplified with the Advantage 2 PCR Kit (Clontech Takara) containing buffer ...
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bioRxiv - Genomics 2019Quote: ... cDNA was synthesized by using SMARTer PCR cDNA Synthesis Kit (Clontech, CA) according to the IsoSeq protocol (Pacific Biosciences ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR product was purified using MiniBEST agarose gel DNA extraction kit (TaKaRa). Following DpnI digestion of the template at room temperature overnight ...
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bioRxiv - Cell Biology 2022Quote: ... followed by cDNA synthesis using SMARTer PCR cDNA synthesis Kit (Takara Bio).
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bioRxiv - Genetics 2020Quote: ... qRT-PCR was performed using SYBR Premix Ex Taq kit (TaKaRa, RR420A) on Agilent Stratagene Mx3005P ...
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bioRxiv - Cancer Biology 2019Quote: Resulting PCR products were gel purified and cloned using InFusion kit (Clontech) into an expression vector containing fabp10a promoter sequence ...
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bioRxiv - Cancer Biology 2019Quote: Resulting PCR products were gel purified and cloned using InFusion kit (Clontech) into an expression vector containing fabp10a promoter sequence ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesized from RNA using SMARTer PCR cDNA synthesis kit (Clontech) per manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: ... and the Quant-X One-Step qRT-PCR TB Green Kit (Takara). A Lenti-X RNA Control Template was provided by the kit and served to build a standard curve of viral genome copies ...
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bioRxiv - Immunology 2021Quote: RACE-PCR was performed using the SMARTer 5’/3’ RACE kit (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The mutants were constructed by conventional PCR using a MutanBEST kit (TaKaRa) and further verified by DNA sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... genomic DNA was purified with Terra™ PCR Direct Genotyping Kit (Takara), followed by PCR amplification and Sanger sequencing of specific genomic regions encompassing the target site.
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bioRxiv - Genomics 2023Quote: ... and extracted using the Nucleospin Gel and PCR Cleanup Kit (Takara, 740609) or Zymoclean Gel DNA Recovery Kit (Zymo Research ...
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bioRxiv - Genetics 2023Quote: ... cDNA was amplified and purified using an Advantage 2 PCR Kit (Clontech). The cDNA library was sequenced using an Illumina sequencing platform (NovaSeq6000) ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was synthesized from RNA using SMARTer PCR cDNA synthesis kit (Clontech) per manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR mix (Takara Bio Inc., Japan), with primers and probe specific for the SARS-CoV-2 E gene ...
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bioRxiv - Developmental Biology 2022Quote: ... Prime Script RT Master Mix (Takara, RR036A) was used to reverse transcribe 1 µg of total RNA into cDNA ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.5 μL PrimeScript RT (Takara, USA), and the reaction mix was incubated at 30 °C for 10 minutes ...
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bioRxiv - Microbiology 2021Quote: ... PrimeScript RT Master Mix (TaKaRa, Cat# RR036A) were used to generate complementary DNAs (cDNAs ...
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bioRxiv - Plant Biology 2022Quote: ... 5 × PrimeScript™ RT Master Mix (TAKARA) was used to synthesize cDNA ...
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bioRxiv - Plant Biology 2024Quote: ... using the PrimeScript RT Master Mix (Takara). We then amplified cDNA in Power SYBR (Thermo ...
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bioRxiv - Neuroscience 2023Quote: ... or the PrimeScript RT Master Mix (Takara). In each individual experiment ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR donor amplicons were purified using the Nucleospin Gel and PCR Clean-up kit (Machery-Nagel, distributed by Takara Bio USA). PCR products were not gel purified ...
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bioRxiv - Bioengineering 2021Quote: ... Viral RNA from pseudovirus infected cells was also quantified by qRT-PCR using the Lenti-X qRT-PCR titration kit (Clontech; 631235) following manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... PCR products to be used as DNA templates were cleaned using a NucleoSpin Gel and PCR Clean-up kit (Takara Bio). In vitro transcription was performed using MAXIscript T7 Transcription Kit (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2024Quote: ... for genotype analysis was extracted using the Qiagen DNeasy Blood and Tissue kit and PCR analysis was done with CloneAmp™ HiFi PCR Premix (Takara) or Phusion polymerase (NEB).
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bioRxiv - Biophysics 2023Quote: ... 5’-CTGGAGAATCCCGGTGC-3’ and 5’- GTGTCAGATATATACATCCTGT-3’ and the PCR product was purified using a NucleoSpin Gel and PCR Clean-up Maxi kit (Takara Bio). The sequences of the 147 bp and 177 bp DNA are as follows:
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... All PCR purifications were performed using the NucleoSpin® Gel and PCR Clean-up kit (TAKARA Bio Inc., San Jose, CA) according to manufacturer protocols ...
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bioRxiv - Cell Biology 2021Quote: Cell lysis was assessed by measuring Lactate Dehydrogenase (LDH) activity in cell supernatants using the LDH cytotoxicity detection kit (TaKaRa). To obtain the positive control ...
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bioRxiv - Immunology 2022Quote: Cell death was measured by quantification of the lactate dehydrogenase (LDH) release into the cell supernatant using LDH Cytotoxicity Detection Kit (Takara). Briefly ...
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bioRxiv - Bioengineering 2022Quote: The cells were confirmed to be mycoplasma-negative every few months using a CycleavePCR Mycoplasma Detection Kit (CY232, Takara Bio).
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bioRxiv - Immunology 2021Quote: ... Cell lysis was quantified by measuring the amount of intracellular LDH release into the cell culture supernatant (TaKaRa LDH cytotoxicity detection kit; Clontech). Percentage PI uptake and LDH release were calculated relative to 100 % cell lysis in untreated control sample.
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bioRxiv - Microbiology 2022Quote: ... and E484Q mutations of the SARS-CoV-2 spike protein were determined by qPCR using mutation detection kits purchased from Takara Bio (Shiga ...
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bioRxiv - Molecular Biology 2022Quote: ... Detection relied on a 32P-labeled DNA probe specific for GFP sequence synthesized with a random priming labeling kit (Takara) followed by exposure to film and development as described (43).
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bioRxiv - Molecular Biology 2022Quote: About 1μg of RNAs were reverse transcribed into cDNAs using a PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Japan) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized from the RNA using the PrimeScriptTM RT Reagent Kit with gDNA Eraser (Takara Bio Inc, Shiga, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... Isolated RNA was reverse transcribed into cDNA was by Prime Script TM RT reagent Kit with gDNA Eraser (Stratagene, Takara). Real-time PCR was performed on ABI-7500 real-time PCR machine (Applied Biosystems ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA was synthesized from 1.0 μg of each RNA sample using PrimeScript RT Kit with gDNA Eraser (TaKaRa, Dalian, China).
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bioRxiv - Developmental Biology 2021Quote: ... Reverse transcription was performed using 2.5 µl of the RT MasterMix (SMART-Seq v5 Ultra Low Input RNA Kit for Sequencing, Takara Bio). cDNA was amplified using 8 µl of the PCR MasterMix (SMART-Seq v5 Ultra Low Input RNA Kit for Sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... were reverse transcribed to complementary DNA (cDNA) using both oligo dT and random primers with PrimeScript RT Reagent Kit (Takara) according to manufacturer’s instructions ...