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Citations for Takara Bio :
1251 - 1300 of 7163 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... First-strand cDNA was synthesized with a PrimeScript™ RT reagent kit with gDNA Eraser (Takara). For gene expression pattern analysis ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription of RNA to cDNA was performed using the PrimeScript RT Reagent kit (RR037A, Takara). Quantitative RT-PCR was then performed on the cDNA using TB Green Premix Ex Taq II (RR820A ...
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bioRxiv - Developmental Biology 2023Quote: ... and reverse transcribed to synthesize cDNA using a PrimeScript RT Master Mix reverse transcription kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Synthesis of cDNA was performed using a PrimeScript™ RT reagent kit (TaKaRa Bio; Tokyo, Japan), and PCR was performed with Taq DNA Polymerase (Toyobo ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was made from 100 or 200 ng of RNA using PrimeScript RT Reagent Kit (TaKaRa). Quantitative PCR was performed using TB Green Premix Ex Taq II (TaKaRa ...
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bioRxiv - Molecular Biology 2020Quote: ... and reverse transcribed into cDNA using a PrimeScript RT RT Master Mix (Takara RR036A). Quantitative real-time RT-PCR analysis was performed using TB Green Premix Ex Taq (Clontech ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted with an Axygen RNA purification kit (Capital Scientific) and the first DNA was synthesized from RNA using a PrimeScript RT regent kit (Takara, Shiga, Japan). Actin-coding gene (KP341672 ...
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The MKK3 MAPK cascade integrates temperature and after-ripening signals to modulate seed germinationbioRxiv - Plant Biology 2024Quote: ... RNA was reverse-transcribed to cDNA by using DNA removal and a cDNA synthesis kit (PrimeScriptTM RT reagent Kit with gDNA Eraser; TaKaRa, Kusatsu, Japan) with a mixture of oligo-dT and random primers ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was removed using the DNA-free kit (Thermo Fischer Scientific, Cat# AMl906) and subsequent cDNA synthesis was performed using the PrimeScript RT reagent Kit (TAKARA, Cat# RR037A), both according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was collected using the Nucleospin RNA Kit (Macherey–Nagel, Hoerdt, France) and transcribed into complementary DNA (cDNA) with the PrimeScript RT Reagent Kit (RR037A, Takara, Kusatsu, Japan). cDNA was then amplified with the SYBR Green Master kit (Roche ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using NucleoSpin Gel and PCR Clean-Up kit (Takara Bio, Kusatsu, Shiga, Japan), and 600 ng-1 ug of DNA templates were added to set up in vitro transcription reactions ...
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bioRxiv - Genomics 2023Quote: ... The obtained PCR products were purified using NucleoSpin Gel and PCR Cleanup kit (Takara Bio, Shiga, Japan) and sequenced directly or were cloned into the pTAC-1 vector (Biodynamics ...
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bioRxiv - Genetics 2023Quote: ... The PCR produc t was purified with a PCR purification kit (Takara Biomedical Technology, Bei jing, China) and was cloned into a pMDTM18-T vector (Takara Biomedical Tec hnology ...
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bioRxiv - Biochemistry 2023Quote: ... PrimeScriptTM RT Master Mix (TaKaRa) was used according to manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2020Quote: ... The cDNA used for qRT-PCR were synthesized using PrimeScript reverse transcriptase with oligo dT primer and Prime Script RT Enzyme MIX I (Takara, Osaka, Japan). qRT-PCRs was performed using the ChamQ SYBR Color qPCR Master Mix (Q411 ...
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bioRxiv - Cancer Biology 2020Quote: ... The ICELL8® 3’ DE Chip was placed on the ICELL8® MSND and loaded 50 µL of RT-PCR solution (Takara Bio ...
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bioRxiv - Plant Biology 2020Quote: The full CDSs of CpPT2 and CpPT3 were amplified from grapefruit flavedo by RT-PCR using TaKaRa Ex Taq polymerase (Takara, Kusatsu, Japan) and the primer pairs for CpPT2 (CpPT2_Fw and CpPT2_Rv ...
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bioRxiv - Molecular Biology 2022Quote: ... The full-length coding region of chicken CDKN3 (accession number: NM_001252162.1) was amplified by RT-PCR and cloned into the empty vector pCMV-HA (Clontech, San Francisco, CA, USA) to construct the overexpression plasmid pCMV-HA-CDKN3 ...
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bioRxiv - Molecular Biology 2020Quote: ... T4 ligase and KOD PCR kit were purchased from Takara Bio ...
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bioRxiv - Neuroscience 2019Quote: ... Amplification was performed using Advantage GC 2 PCR kit (Clontech) and PCR products were cloned and sequenced.
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bioRxiv - Immunology 2022Quote: ... A high-fidelity Advantage HF2 PCR kit (Takara, Cat#639123) was used in each of the PCR steps involved in preparing the sequencing libraries ...
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bioRxiv - Molecular Biology 2023Quote: ... purified using NucleoSpin Gel and PCR Clean-Up Kit (Takara), cloned into pRACE vector (provided in the kit ...
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bioRxiv - Plant Biology 2023Quote: ... and standard PCR was performed using ExTaq HS kit (TaKaRa). Control reactions were run using wild-type DNA as a template.
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bioRxiv - Cell Biology 2023Quote: ... by PCR using a PrimeSTAR Mutagenesis Basal Kit (Takara, R046A) and the following primers ...
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bioRxiv - Neuroscience 2024Quote: ... and the Advantage 2 PCR kit (Takara/Clontech Cat # 639207). Libraries were checked for quality and approximate concentration on a 1% agarose gel.
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bioRxiv - Neuroscience 2024Quote: ... and the Advantage 2 PCR kit (Takara/Clontech Cat # 639207). Libraries were checked for quality and approximate concentration on a 1% agarose gel.
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bioRxiv - Microbiology 2024Quote: ... Conventional PCR was performed with TaKaRa EX Taq kit (TaKaRa) and included 5 µL 10X PCR Buffer (Mg2+ plus) ...
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bioRxiv - Cell Biology 2024Quote: ... was amplified and analysed in real-time polymerase chain reaction on a Roche Light Cycler 480 system with TB Green® Premix Ex Taq™ II (TaKaRa Bio Inc., Japan). The specific primers were predesigned and validated by PrimerBank of Massachusetts General Hospital (Spandidos et al. ...
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bioRxiv - Biochemistry 2020Quote: ... and cell toxicity was evaluated using the LDH Cytotoxicity Detection Kit (Cat #MK401; Takara Bio, Shiga, Japan).
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bioRxiv - Neuroscience 2024Quote: ... followed by a T7 endonuclease I (T7EI) assay using the Guide-it Mutation Detection Kit (Takara Bio) following the standard protocol for mismatch detection.
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bioRxiv - Biophysics 2021Quote: ... The first-strand cDNA was synthesized using a PrimeScript® RT reagent Kit (Takara, Otsu, Shiga, Japan). The PlMYB308 gene was PCR-amplified using specific primers (Table S6) ...
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bioRxiv - Immunology 2021Quote: ... Reverse transcription was performed with a PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Cat no. RR047A) and qRT-PCR was performed on a StepOnePlus Real-time PCR system (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA synthesized from 1 μg total RNA using PrimeScript™ RT reagent Kit with gDNA Eraser (Takara). Real-time PCR was performed using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...
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Mutant ACTB mRNA 3′UTR Promotes Hepatocellular Carcinoma Development by Regulating miR-1 and miR-29abioRxiv - Cancer Biology 2019Quote: ... the isolated RNA was reverse transcribed to cDNA using a PrimeScript RT reagent Kit (Takara, Dalian China). Real-time PCR analyses were performed with SYBR Green (Takara ...
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bioRxiv - Developmental Biology 2019Quote: ... and then cDNA was synthesized using the PrimeScript reverse transcription (RT) reagent kit (6210B; Takara Bio. Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... total RNA was used for reverse transcription (RT) using the SMARTScribe Reverse Transcription Kit (TaKaRa, Cat #639537).
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bioRxiv - Cancer Biology 2019Quote: ... and subsequently subjected to reverse transcription using PrimeScript™ RT reagent Kit (TaKara Bio, Mountain View, CA). Quantitative real-time PCR was performed with Premix Ex Taq (TaKara Bio ...
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bioRxiv - Immunology 2022Quote: ... 1 μg of total RNA was used to synthesize cDNA with PrimeScript RT reagent kit (RR047A, Takara) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... total RNA was reverse transcribed to cDNA using the PrimeScript™ RT reagent Kit (TaKaRa, RR037A, Japan). Convergent and divergent primers were used to detect the expression of linear and circular RNA transcripts ...
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bioRxiv - Plant Biology 2021Quote: ... gDNA removal and first-strand cDNA synthesis were conducted by PrimeScriptTM RT reagent Kit (Takara, Beijing, China) with 0.5 μg total RNA for each sample ...
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bioRxiv - Neuroscience 2020Quote: ... spectrophotometer followed by reverse transcription reaction to produce cDNA using PrimeScript RT Reagent Kit (Takara, Clonetech, Japan). Specific primers against the genes of interest (Table 3 ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription was performed with a PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Cat no. RR047A) and qRT-PCR was performed on StepOne Plus Real-time PCR system (Applied Biosystem ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized from equivalent total RNA using PrimeScript™ RT reagent Kit with gDNA Eraser (TAKARA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... and cDNA was synthesized with the PrimeScript RT reagent Kit with the gDNA Eraser (TaKaRa Bio Inc.). Real-time PCR was performed using the KOD SYBR qPCR Mix (TOYOBO ...
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bioRxiv - Cell Biology 2019Quote: ... Extracted total RNA were quantified and reverse transcribed into cDNA using the PrimeScript RT Reagent Kit (TaKaRa). All qPCR reactions were performed as 10 μl reactions using TB Green™ Premix Ex Taq™ II (TaKaRa) ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 500 ng total RNA using PrimeScript RT reagent Kit (Takara Bio., Shiga, JAPAN). The absence of genomic DNA contamination was confirmed by PCR using a control without reverse transcriptase ...
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bioRxiv - Genetics 2020Quote: ... according to the manufacturer’s instructions and cDNAs were prepared using PrimeScriptTM RT Master Mix kit (Takara, RR036A). Analysis of mRNA expression was performed with SYBR Green Master Mix (Life Technologies ...
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bioRxiv - Physiology 2021Quote: 200 ng of mRNA were reverse-transcribed to cDNA using the PrimeScriptTM RT reagent kit (RR037A; Takara). Fluorescence-based quantitative real-time PCR (qRT-PCR ...
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Transcriptome Analysis of xa5-mediated Resistance to Bacterial Leaf Streak in Rice (Oryza sativa L.)bioRxiv - Plant Biology 2019Quote: ... First-strand cDNA synthesis was performed using PrimeScript™ RT reagent kit with gDNA eraser (Takara, Japan) following the manufacturer’s instruction ...
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bioRxiv - Genomics 2021Quote: ... RNAs were reverse transcribed by using the PrimeScript RT reagent Kit with gDNA Eraser (TAKARA, Kyoto, Japan). Quantification of candidate genes was conducted in triplicate by using Bio-rad iQ SYBR Green Supermix (Bio-rad ...