Labshake search
Citations for Takara Bio :
1001 - 1050 of 2391 citations for PCR Tube since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: Coding sequence of CROCCP2 was amplified by PCR using Prime Star DNA polymerase (TAKARA) from GW9 human fetal cortex cDNA ...
-
bioRxiv - Cancer Biology 2020Quote: ... The amplified PCR product was inserted into EcoRI-linearized pLVX TetOne-puro plasmid (Clontech) using the NEBuilder HiFi Assembly kit (New England BioLabs) ...
-
bioRxiv - Biochemistry 2021Quote: ... mutations were introduced into the RSF-OxlT vector via PCR using PrimeSTARMax (Takara Bio). Protein production was induced by adding 1 mM IPTG and 10 µM all-trans retinal (Sigma ...
-
bioRxiv - Microbiology 2021Quote: ... we performed RACE PCR using a SMARTer® RACE 5’/3’ Kit (Takara Bio), according to the manufacturer’s specifications ...
-
bioRxiv - Biochemistry 2020Quote: ... The product was cloned into pET28a plasmid using In-Fusion PCR Cloning Kit (Clontech), with an N-terminal hexahistidine (His6 ...
-
bioRxiv - Bioengineering 2020Quote: ... Then qPCR was conducted with a One-Step PrimeScrip RT-PCR Kit (Takara, Japan), following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... and purified with NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 74061) for Sanger sequencing ...
-
bioRxiv - Neuroscience 2022Quote: ... The titer was measured using a Lenti-X qRT-PCR titration kit (Clontech 631235).
-
bioRxiv - Developmental Biology 2022Quote: ... The qRT-PCR was performed with the cDNA using the SYBR-green stain (Takara). Primers used for qPCR are listed below.
-
bioRxiv - Developmental Biology 2022Quote: ... The titer of retrovirus was measure by Retro-X qRT-PCR Titration Kit (Clontech). For iPSC generation ...
-
bioRxiv - Immunology 2022Quote: ... the PCR product was assembled into an EcoRI + XhoI digested pMSCV-neo (Takara Clontech) vector by using Gibson Assembly Master Mix (NEB ...
-
bioRxiv - Immunology 2022Quote: ... PCR amplicons were purified from 1% agarose gels following electrophoresis by column purification (Takara) and submitted for sanger sequencing through Genewiz (Primer ...
-
bioRxiv - Cell Biology 2022Quote: qRT-PCR were performed using the SYBR Premix Ex Taq polymerase (Takara Bio Inc) with the ViiA™7 Instrument (Applied Biosystems) ...
-
bioRxiv - Cell Biology 2019Quote: ... Human B55α was PCR amplified from HUVEC cDNA and cloned into pEGFP-C1 (Clontech) using HindIII and BamHI sites ...
-
Biosynthesis of Circular RNA ciRS-7/CDR1as Is Mediated by Mammalian-Wide Interspersed Repeats (MIRs)bioRxiv - Molecular Biology 2019Quote: ... and cDNA was amplified by PCR (20–30 cycles) with Ex-Taq (Takara Bio) and specific primers (Table S1) ...
-
bioRxiv - Pathology 2019Quote: ... Quantitative PCR (qPCR) was performed using TB GreenTM Premix Ex TaqTM II (TaKaRa, RR820A) on QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Plant Biology 2019Quote: ... Resulting PCR products were cloned into EcoRI/SalI-digested pBD-GAL4 (Clontech, CA, USA) using standard restriction ligation ...
-
bioRxiv - Cancer Biology 2019Quote: ... qPCR was performed with a StepOnePlus™ Real-Time PCR Systems from Applied Biosystems and the SYBR Green PCR Core reagents kit (Takara, Ozyme, Saint Cyr L’Ecole, France). All RNAs for the miRNA investigation were transcribed using miScript RT kits and subsequent qPCR performed using miScript Primer Assays and miScript SYBR kits (Qiagen ...
-
bioRxiv - Cancer Biology 2019Quote: ... Real-Time PCR was performed using SYBR Premix EX Taq kit (Takara, Shiga, Japan). The specific primers were used to detect the relative mRNA expression of IL-1RA by the 2 - ct method ...
-
bioRxiv - Cell Biology 2019Quote: ... PCR (25 cycles) was performed to amplify the sgRNAs using Ex Taq Polymerase (Takara), in total 65 tubes for control cells and 12 tubes for sort3 cells (Oligos for amplification of sgRNAs are in Supplementary Table 1) ...
-
bioRxiv - Molecular Biology 2020Quote: The cleavage substrate was amplified using overlap-PCR (PrimeSTAR GXL DNA Polymerase from Takara) from the random PAM plasmid library (see Supplementary Materials and Methods) ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... Quantitative real-time PCR (qPCR) was done using Ex Taq SYBR Green (Takara-RR820A), gene specific primers (Table 1 ...
-
bioRxiv - Cancer Biology 2020Quote: ... PCR products were cloned into the T-vector pMD19 (TAKARA Bio Inc, Cat#3271). Plasmid DNA was extracted and sequenced with M13 forward or reverse primers (Eton Bioscience Inc ...
-
bioRxiv - Developmental Biology 2020Quote: ... The PCR reaction system was prepared with a kit (SYBR Premix Ex Taq, Takara), and the reaction was performed on an Mx3000P real-time PCR machine (Agilent Stratagene ...
-
bioRxiv - Developmental Biology 2021Quote: ... cDNA was synthesized with the Clontech SMARTer PCR cDNA Synthesis Kit (Takara Bio Inc). RNA was also extracted from dissected brains of one-day old paralarvae in a similar manner and cDNA was synthesized using the NEBNext cDNA Synthesis & amplification kit ...
-
bioRxiv - Molecular Biology 2020Quote: ... These cell lines were tested negative for mycoplasma (TaKaRa PCR Mycoplasma Detection Set; 6601), maintained in RPMI1640 (Nacalai Tesque ...
-
bioRxiv - Cell Biology 2020Quote: ... 68℃ for 1min (Fig. 1C) or using EmeraldAmp GT PCR Master Mix (Takara Bio) for 30 cycles of 98℃ for 10s ...
-
bioRxiv - Genetics 2021Quote: ... Fragments after electrophoresis were recovered using NucleoSpin Gel and PCR Clean-up (Takara Bio). The nucleotide sequences were determined by the Yamaguchi University Center for Gene Research ...
-
bioRxiv - Molecular Biology 2021Quote: ... and then used as a template for PCR for 35 cycles with EpiTaq (TAKARA) using the following primer ...
-
bioRxiv - Cancer Biology 2021Quote: ... and then gel purified using NucleoSpin® Gel & PCR Clean-up Midi (Takara # 740986.20) for deep sequencing using the CRISPR-seq platform117 ...
-
bioRxiv - Zoology 2019Quote: ... High-fidelity Takara Ex Taq PCR reagents were employed (Takara Bio Inc., Otsu, Japan) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... PCR on the resulting cDNA was performed for 35 cycles using PrimeStar MAX (Takara). Then ...
-
bioRxiv - Microbiology 2019Quote: ... PCR for SNVs at IDEXX BioAnalytics used LA Taq™ (TaKaRa Bio, Otsu, Japan) and 20 µM primer pairs ...
-
bioRxiv - Microbiology 2019Quote: ... The purified PCR products were sequenced with a commercial sequencer (Takara Bio, Shiga, Japan) by fluorescent dye terminator sequencing ...
-
bioRxiv - Molecular Biology 2019Quote: ... All constructions were obtained by assembling PCR fragments using InFusion® cloning kit (Takara). Using appropriate sets of oligonucleotides ...
-
bioRxiv - Molecular Biology 2019Quote: ... all other PCR products were amplified with PrimeSTAR HS DNA polymerase (Clontech Laboratories, Inc.). DNA isolations and manipulations were carried out using standard protocols ...
-
bioRxiv - Genetics 2020Quote: ... double strand DNA was synthesized by PCR using PrimeSTAR GXL DNA Polymerase (Takara Bio) using forward 5’-ATGTGGTCGCGCCTGGCCTTTTGTTG and reverse 5’-AAGCCCGGCTCTCCTTTTCCTGGAACAATCAT primers ...
-
bioRxiv - Cell Biology 2020Quote: ... Relative quantification PCR were performed using TB Green Premix Ex Taq kit (Takara, RR820B) and the StepOnePlus Real-Time PCR System (ABI ...
-
bioRxiv - Cell Biology 2021Quote: ... The PITCh-mNeonGreen-guideMYH9 was generated by PCR and In- Fusion cloning method (Takara) to insert mNeonGreen at the 3’ end of MYH9 coding sequence with 40-bp microhomologies in accordance with the previous reports (Sakuma et al. ...
-
bioRxiv - Cancer Biology 2020Quote: ... Tagmented DNA was isolated by Nucleospin PCR clean-up (Takara Bio USA, Inc # 740609.250). Libraries were amplified for 8 cycles and purified using AMPure XP (Agencourt # A63880) ...
-
bioRxiv - Cell Biology 2020Quote: ... The cDNA was amplified using a fluorescent quantitative polymerase chain reaction (PCR) kit (TaKaRa) and an instrument (Applied Biosystems 7500 ...
-
bioRxiv - Biochemistry 2021Quote: ... real-time polymerase chain reaction (PCR) amplification was performed with SYBR Premix ExTag (Takara) on a QuantStudio™ 3 System (Applied Biosystems) ...
-
bioRxiv - Microbiology 2021Quote: ... PCR reactions for amplifying deletion cassettes were done using PrimeSTAR MAX DNA Polymerase (Takara). NAD+ and NADP+(Na)2 were purchased from Carl Roth GmbH ...
-
bioRxiv - Microbiology 2020Quote: ... PCR amplification was achieved using TaKaRa Ex Taq DNA Polymerase (ClonTech, Mountain View, CA). This amplified PCR product was then cloned into the pGEM-T Easy Vector using T4 ligase (Promega ...
-
bioRxiv - Molecular Biology 2020Quote: ... and Q263A mutants were constructed by inverse PCR using PrimeSTAR MAX (Takara, Tokyo, Japan). For crystallization ...
-
bioRxiv - Immunology 2021Quote: ... Real-time PCR were performed with the SYBR Premix Ex Taq II (TAKARA#RR820A) in a LightCycler480 real-time PCR system ...
-
bioRxiv - Cancer Biology 2021Quote: ... Quantitative real-time PCR (qPCR) was performed with TB Green Premix Ex Taq (Takara) using LightCycler 96 System (Roche Life Science ...
-
bioRxiv - Immunology 2022Quote: ... by PCR and subcloned in pEGBacMam using In-Fusion seamless DNA cloning (Takara Bio). PCR was used to generate the pEGBacMam-Strep-FLAG-Strep-EROS construct ...
-
bioRxiv - Genetics 2022Quote: ... Cas9 was amplified from the expression plasmid using CloneAmp HiFi PCR Premix (Takara Bio) for 35 cycles (98□ for 10 s ...
-
bioRxiv - Bioengineering 2022Quote: ... 1 µl purified cDNA was amplified with an Advantage HF 2 PCR kit (Takara) in a 25 µl reaction containing 0.5 µl forward primer (10 µM ...