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Citations for Takara Bio :
951 - 1000 of 2518 citations for PCR Tube since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... then ligating both PCR products by In-Fusion (Takara Bio, Mountain View, CA), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... BacT/ALERT 3D Microbial Identification System and PCR Mycoplasma Detection Set (Takara, 6601) were applied to detect bacterial/fungi and mycoplasma contamination ...
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bioRxiv - Neuroscience 2021Quote: ... One microgram of cDNA was added to the PCR-reaction premix (Takara Bio) with 10 pM corresponding primer pairs ...
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bioRxiv - Cell Biology 2020Quote: ... by PCR and inserted to vectors peGFP-C2 and pmRFP1-C2 (originally Clontech), designed for expression of protein chimeras with a fluorescent protein connected to the N-terminus of the target protein ...
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bioRxiv - Biophysics 2020Quote: ... The mCherry gene (AgeI / NheI) was obtained by PCR on pmCherry-N1 (Clontech). A four-fragment-ligation was then done to obtain the final construct (HindIII-HA-Nlg1-AgeI-mCherry-NheI-Nlg1Cter-NotI) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Long-range PCRs were performed with PrimeSTAR GXL DNA Polymerases (Takara Bio Europe). Initial sequences generated following Bourguignon et al ...
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bioRxiv - Plant Biology 2021Quote: ... the coding regions of RSL4 (AT1G27740.1) were amplified by PCR (PrimeStar max, Takara) using genomic DNA and cDNA as templates ...
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bioRxiv - Immunology 2021Quote: ... PCR was performed with 2x SeqAMP buffer and SeqAmp DNA polymerase (Takara USA) and for the final amplification ...
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bioRxiv - Immunology 2021Quote: ... PCR was performed with 2x SeqAMP buffer and SeqAmp DNA polymerase (Takara USA) and for the final amplification ...
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bioRxiv - Microbiology 2021Quote: ... using random hexamers and PCR was performed with PrimeSTAR Max DNA Polymerase (Takara). Primers were designed based on the assembled viral contigs ...
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bioRxiv - Microbiology 2020Quote: ... RT-PCR was accomplished using the SYBR premix Ex Taq kit (Takara, China) on an ABI StepOnePlus Real-Time PCR System (Thermofisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Nested PCR was performed using TaKaRa Ex Taq (Takara Bio Inc., Shiga, Japan). The initial and nested PCR protocols have been described elsewhere(46 ...
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bioRxiv - Immunology 2020Quote: ... RT-qPCR was performed using One Step PrimeScript RT-PCR Kit (Takara, Japan) with the following primers and probes ...
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bioRxiv - Immunology 2020Quote: ... Total RNA was reverse-transcribed using mRNA Selective PCR kit (TaKaRa Bio-Clontech). Mouse integrin beta 1 ...
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bioRxiv - Immunology 2020Quote: ... Total RNA was reverse-transcribed using mRNA Selective PCR kit (TaKaRa Bio-Clontech). Mouse integrin beta 1 ...
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bioRxiv - Microbiology 2021Quote: ... PCR amplifications were carried out with Phusion polymerase (Finnzymes) or PrimestarMAX (Takara Clontech) as recommended by the manufacturers ...
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bioRxiv - Microbiology 2021Quote: ... PCR amplifications were carried out with Phusion polymerase (Finnzymes) or PrimestarMAX (Takara Clontech) as recommended by the manufacturers ...
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bioRxiv - Developmental Biology 2020Quote: ... Real-time PCR was performed using SYBR Premix Ex Taq II (Takara Bio) on an ABI 7500 Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed using One Step PrimeScript RT-PCR Kit (Takara, Japan) with the following primers and probes 55 ...
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bioRxiv - Genetics 2020Quote: ... PCR amplification was performed with TaKaRa LA Taq Hot Start polymerase (TaKaRa Biotechnology) under conditions of ...
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bioRxiv - Microbiology 2020Quote: ... Following PCR amplification with TaKaRa Ex Taq DNA Polymerase (ClonTech, Mountain View, CA), the PCR product and pRRK plasmid were digested with the XBaI restriction endonuclease (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... The ORF3b derivatives were generated by PCR using PrimeSTAR GXL DNA polymerase (Takara), the synthesized ORFs as templates ...
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bioRxiv - Synthetic Biology 2022Quote: ... PrimeStar DNA polymerase and SapphireAmp* Fast PCR Master Mix were purchased from TaKaRa Bio ...
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bioRxiv - Cancer Biology 2024Quote: ... and first-strand cDNA was prepared using an AMV RNA PCR kit (TaKaRa) from 1 mg of total RNA ...
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bioRxiv - Neuroscience 2022Quote: ... PCR was performed using the TB Green Ex Taq II Mix (Takara Bio) and the Thermal Cycler Dice Real-Time System (Takara Bio).
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bioRxiv - Neuroscience 2022Quote: ... DNA was purified using NucleoSpin Gel and PCR Clean-up kits (Takara, 740609.250). Libraries were prepared identically to SHARE-seq ATAC libraries.
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bioRxiv - Microbiology 2022Quote: ... This cassette was further modified by PCR and circularized by recombination (InFusion Takara) to replace gRNA1-tracerRNA1-gRNA2 by a NotI site ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was then reverse transcribed by the SMARTer PCR cDNA Synthesis Kit (Clontech) and amplified for 15 cycles using KAPA HiFi PCR Kit (KAPA biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... and 2 µl of lysate was used for PCR using SapphireAMP (Takara, RR350) and primers specific for each gene ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized using the SMARTer PCR cDNA Synthesis Kit (Takara Bio Group) and sequenced using the Illumina HiSeq 2000 sequencer at the Beijing Genomics Institute (BGI ...
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bioRxiv - Genetics 2024Quote: ... PCR amplifications were done in duplicates using the PrimerSTAR GXL polymerase (Takara Bio) and 250 ng of genomic DNA per reaction ...
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bioRxiv - Genetics 2024Quote: ... Real-time PCR was carried out using SYBR Premix Ex TaqTM II (Takara) on the Applied Biosystems StepOne Real-Time PCR System ...
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bioRxiv - Genomics 2023Quote: ... Products were purified with the Nucleospin Gel and PCR Cleanup Kit (Takara, 740609) with 1:5 buffer NTI dilution and 25 µL 70 °C elution buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... sgRNA inserts were PCR amplified using Ex Taq DNA Polymerase (Takara, cat#RR001A) from sufficient genome equivalents of DNA to achieve an average coverage of >200x of the sgRNA library ...
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bioRxiv - Cell Biology 2024Quote: ... Flanking att sequences were added through another round of PCR (Takara, cat # R011) and purified ...
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bioRxiv - Plant Biology 2024Quote: ... The PCR reaction was performed using PrimeSTAR Max DNA Polymerase (Takara Bio., Japan) with the following thermal cycling parameters ...
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bioRxiv - Neuroscience 2024Quote: ... Titer was determined using Lenti-X qRT-PCR Titration Kit (Takara Bio 631235). pLV-dCas9-p300-P2A-PuroR was a gift from Charles Gersbach (Addgene plasmid # 83889 ...
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bioRxiv - Developmental Biology 2024Quote: ... These PCR fragments were fused with the In-Fusion HD Cloning kit (Clontech). The region encoding XY linker43 was deleted from PLCβ1/2/3 cDNA by inverse PCR to create a constitutively active form ...
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bioRxiv - Cancer Biology 2023Quote: ... Polymerase chain reaction (PCR) was performed using PrimeSTAR GXL DNA Polymerase (TaKaRa, R050A), following the recommended Rapid PCR protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... A second 23-25 cycles PCR was performed with PrimeStar HS polymerase (Takara) or KAPA HiFi DNA Polymerase (Roche) ...
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bioRxiv - Genetics 2023Quote: ... real-time PCR was performed with SYBR Premix Ex Taq II (TaKaRa, China) on an iCycler RT‒PCR Detection System (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... All PCRs were performed using PrimeSTAR Max polymerase (Takara Bio, catalog no. R045B) per the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... sgRNA inserts were PCR amplified using Ex Taq DNA Polymerase (Takara, cat#RR001A) from sufficient genome equivalents of DNA to achieve an average coverage of >200x of the sgRNA library ...
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bioRxiv - Microbiology 2022Quote: ... We performed the second round of PCR using Premix Taq™ (TAKARA, RR902Q) in a 50μl reaction volume ...
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bioRxiv - Bioengineering 2023Quote: ... qRT-PCR was performed with TB Green Premix Ex Taq II (Takara RR820L). Each 20-μl reaction contained 2 μL of cDNA and 10 μL of TB Green solution with a final primer concentration of 200 nM ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged mCherry (mCherry-His) was PCR amplified from pmCherry-C1 vector (Takara) templates with primers encoding a His-tag and NcoI/NotI cut sites (Takara) ...
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The arms race in noncoding regions of transposable element: the evolution of anti-silencing and RNAibioRxiv - Genetics 2022Quote: ... Converted DNA was used as a template for PCR using EpiTaq HS (TaKaRa). The PCR conditions were 94 °C for 1 min ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Amplified products were purified using NucleoSpin Gel and PCR Clean-up (Takara Bio), and cloned into the pGEM-T easy vector (Promega) ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative real-time PCR was carried out using 2× TB-Green premix (TaKaRa) on a LightCycler-480®II (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR reaction mixture for all reactions consisted of 5x PrimeSTAR Buffer (Takara), 200uM dNTPs (Takara) ...