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Citations for Takara Bio :
851 - 900 of 2391 citations for PCR Tube since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... Quantitative (q) RT-PCR was performed using SYBR Premix ExTaq 11 (Takara Bio) and detected using the Bio-Rad CFX96 Connect system ...
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bioRxiv - Biochemistry 2022Quote: ... qRT-PCR was performed with SYBR Premix Ex TaqII (RR820A, Takara Bio Inc.) in an ABI 7300 thermal cycler (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... and amplified genomic DNA by PCR using the PrimeSTAR HS DNA Polymerase (TaKaRa) or KOD-plus Ver ...
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bioRxiv - Cancer Biology 2022Quote: ... and gel extracted using the NucleoSpin Gel and PCR Clean-up kit (Takara Bio USA Inc ...
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bioRxiv - Developmental Biology 2022Quote: ... Quantitative PCR was performed using a TB Green Premix DimerEraser Kit (RR091A; Takara). The primer sequences are listed in Supplementary Table S1.
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TIR signaling promotes the interactions between EDS1/PAD4 and ADR1-L1 and oligomerization of ADR1-L1bioRxiv - Plant Biology 2021Quote: ... Real-time PCR was performed using a SYBR premix kit (TaKaRa, Cat#RR82LR). The primers used are listed in Supplemental Table 1.
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bioRxiv - Microbiology 2021Quote: ... PCR products were cloned into pGE450 using the In-Fusion cloning system (Takara). Start codon mutant constructs were made by inverse PCR-amplification of the wild-type constructs using primers that introduce a start codon mutation (“T” to “C” change at the central position of the start codon ...
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bioRxiv - Molecular Biology 2019Quote: LncRNA expression was measured using the SYBR® Green PCR Kit (Takara, Japan) in a blinded fashion ...
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bioRxiv - Microbiology 2021Quote: ... Real-time PCR was performed using SYBR Premix Ex Taq II (TaKaRa, RR820A) on LightCycler96 qPCR system (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... The amplicon was purified by NucleoSpin® Gel and PCR Clean-Up (Takara) for Sanger sequencing.
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bioRxiv - Biophysics 2020Quote: ... PCR-based site-directed mutagenesis with In-Fusion HD Cloning system (Takara, Clonetech) was used to generate PscD D43A ...
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bioRxiv - Microbiology 2020Quote: ... The reactions were performed using a TaKaRa PCR Thermal Cycler (TaKaRa, Kyoto, Japan) and then run on a 1.2%agarose/TBE gel.
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bioRxiv - Immunology 2020Quote: ... Real-time PCR was carried out with the Premix Ex Taq (Takara, Japan) to quantify the expression level of miR-137 and miR-7847 ...
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bioRxiv - Molecular Biology 2020Quote: ... VAL2 and VAL3 were amplified by PCR (PrimeSTAR TM HS DNA Polymerase, TaKaRa) using the plasmids carrying the full-length cDNA of each gene as template ...
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bioRxiv - Immunology 2020Quote: ... The subsequent 8 cycles PCR reaction was performed with the PrimeStarMax polymerase (Takara) using primers ...
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bioRxiv - Immunology 2020Quote: ... and 300-500ng genomic DNA was amplified by PCR using PrimeSTAR MAX (Takara) for 30-35cyles using the following primers ...
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bioRxiv - Immunology 2020Quote: ... qRT-PCR was performed using TB Green TM Premix Ex TaqTM II (Takara). The following was the reaction system ...
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bioRxiv - Microbiology 2021Quote: ... cDNA molecules were amplified using the Advantage 2 PCR kit (639206, Takara Bio) with initial denaturation at 95 °C for 1 min ...
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bioRxiv - Genomics 2022Quote: ... and elute was concentrated with NucleoSpin Gel and PCR Clean-Up (Takara Bio). The pooled samples were sequenced using 5 lanes of Illumina Nextseq 550 High-Output ...
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bioRxiv - Genomics 2022Quote: ... PCR amplification was performed for 8-9 cycles using PrimeSTAR GXL Polymerase (Takara) at an annealing temperature of 60°C ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA was purified using the NucleoSpin Gel & PCR Clean-up Kit (Takara, 740986.20) and 100 bp paired end reads sequenced using Illumina NovaSeq at the Integrated Genomics Operation at MSKCC ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA fragments containing eIF4A iso4 were PCR-amplified and inserted into pColdI (TaKaRa) downstream of the His tag with In-Fusion HD (TaKaRa) ...
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bioRxiv - Bioengineering 2019Quote: ... and Cd200 were amplified by extension PCR using PrimeStar HS mastermix (Takara R040) and gateway compatible attb1/attb2 sites were added to the 5’end and 3’ ends ...
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bioRxiv - Genomics 2019Quote: ... The IRES2 fragment was PCR amplified from the pTRE3G-IRES (Cat. # 631174, Clontech) using the primer pairs
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bioRxiv - Evolutionary Biology 2019Quote: ... These PCRs were performed with the Advantage2 Taq Polymerase (Clontech, Mountain View, CA) for high fidelity ...
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bioRxiv - Genetics 2019Quote: ... Real-time PCR was performed using a SYBR Premix Ex TaqTM kit (TaKaRa) on an ABI prism 7500 real-time PCR System and three biological repeats ...
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bioRxiv - Neuroscience 2019Quote: ... Physical viral titer was determined using Lenti-X qRT-PCR Titration Kit (Takara), and only viruses greater than 1×1011 GC/ml were used ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... The PCR products were directly ligated into PMD19-T simple vector (TaKaRa, China) and then transformed into Escherichia coli BL21 competent cells (Transgen ...
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bioRxiv - Biochemistry 2019Quote: ... Any additional plasmids were constructed using backbone or insert PCR and inFusion (Clontech). Stellar competent cells were transformed by all the plasmids for amplification and DNA storage.
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bioRxiv - Genetics 2021Quote: ... The BE2+Cas9m4 insert was PCR amplified and cloned using in-Fusion (Clontech) into the pActin5c-attB-RFP vector isolated by digestion of pActin5c-Cas9 (Addgene# 62209 (Port et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative RT-PCR was performed using TB Green Premix Ex Taq II (Takara). Relative gene expression was normalized to ACTB expression ...
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bioRxiv - Microbiology 2021Quote: ... The gene quantitative PCR was performed using TB green Premix EX Taq (Takara) with a QuantStudio 6 Flex (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... PCR was performed with PrimeSTAR Max DNA polymerase (Takara Bio Inc, Shiga, Japan) with the following parameters ...
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bioRxiv - Microbiology 2020Quote: ... and 2 µl of lysate was used for PCR using SapphireAMP (Takara, RR350) and gene-specific primers ...
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bioRxiv - Synthetic Biology 2020Quote: ... Polymerase chain reaction (PCR) fragments were generated using PrimeSTAR Max DNA Polymerase (TaKaRa). The method for plasmid construction is described below.
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bioRxiv - Bioengineering 2021Quote: Polymerase Chain Reactions (PCR) were conducted using PrimeSTAR GXL DNA Polymerase (Takara Bio) per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... FveGID1c were PCR amplified and ligated into GAL4 AD vector PGADT7 (Clontech Inc.) at the BamHI/XhoI ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The PCR reaction included 2.5 µL of 10X buffer (Mg2+ plus) (TaKaRa, Japan), 1.5 µL of 25 mM MgCl2 ...
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bioRxiv - Microbiology 2019Quote: ... All PCR reactions were carried out with the Takara ExTaq (Takara, Kusatsu, Japan) reaction kit ...
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bioRxiv - Genetics 2021Quote: ... Quantitative RT-PCR was performed by using SYBR Premix Ex TaqTM (TaKaRa Bio) on a ABI StepOnePlusTM Real-Time PCR thermocycler (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... Quantitative RT-PCR was performed by using SYBR Premix Ex TaqTM (TaKaRa Bio) on an ABI StepOnePlusTM Real-Time PCR thermocycler (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... PCR products were used for sub-cloning into the pMD18-T vector (Takara) and sequenced to determine mutation efficiency ...
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bioRxiv - Microbiology 2021Quote: ... then ligating both PCR products by In-Fusion (Takara Bio, Mountain View, CA), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... BacT/ALERT 3D Microbial Identification System and PCR Mycoplasma Detection Set (Takara, 6601) were applied to detect bacterial/fungi and mycoplasma contamination ...
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bioRxiv - Neuroscience 2021Quote: ... One microgram of cDNA was added to the PCR-reaction premix (Takara Bio) with 10 pM corresponding primer pairs ...
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bioRxiv - Cell Biology 2020Quote: ... by PCR and inserted to vectors peGFP-C2 and pmRFP1-C2 (originally Clontech), designed for expression of protein chimeras with a fluorescent protein connected to the N-terminus of the target protein ...
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bioRxiv - Biophysics 2020Quote: ... The mCherry gene (AgeI / NheI) was obtained by PCR on pmCherry-N1 (Clontech). A four-fragment-ligation was then done to obtain the final construct (HindIII-HA-Nlg1-AgeI-mCherry-NheI-Nlg1Cter-NotI) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Long-range PCRs were performed with PrimeSTAR GXL DNA Polymerases (Takara Bio Europe). Initial sequences generated following Bourguignon et al ...
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bioRxiv - Plant Biology 2021Quote: ... the coding regions of RSL4 (AT1G27740.1) were amplified by PCR (PrimeStar max, Takara) using genomic DNA and cDNA as templates ...
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bioRxiv - Immunology 2021Quote: ... PCR was performed with 2x SeqAMP buffer and SeqAmp DNA polymerase (Takara USA) and for the final amplification ...