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Citations for Lonza :
4301 - 4350 of 4910 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... cultured cells were washed by dispensing and aspirating 37°C HEPES buffered saline solution (Lonza, CC-5022) and then dissociated with 0.025% Trypsin/EDTA (Lonza ...
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bioRxiv - Immunology 2024Quote: ... Non-diseased non-parenchymal cells (NPCs) were purchased from Triangle Research Laboratories (Lonza, Triangle research park, NC). All patients provided written and informed consent and the study was approved by the institutional review board at the University of Colorado—Anschutz.
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... MilliporeSigma) plates with a density of 20,000 cells/cm2 with Endothelial Growth Medium-2 (EGM-2; Lonza), which consists of Endothelial Growth Basal Medium-2 (EBM-2 ...
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bioRxiv - Cell Biology 2024Quote: ... HMVEC-L were cultured in EGMTM-2MV Microvascular Endothelial Cell Growth Medium-2 BulletKitTM (Lonza, CC-3202) at 37°C ...
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bioRxiv - Immunology 2024Quote: ... rMASPs were expressed in CHO-DG44 cells cultivated in Power CHO serum-free media (Lonza, Vallensbaek, Denmark) supplemented with 20 U/mL penicillin ...
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bioRxiv - Immunology 2024Quote: ... Wien-133 (human CD52-positive Burkitt’s lymphoma) cells were cultured in Iscove’s Modified Dulbeco’s Medium (IMDM) with HEPES and L-Glutamine (Lonza), supplemented with 10% (v/v ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were split at a 1:2 ratio every 2 days by trypsinization (Lonza cat# CC-5012), followed by centrifugation at 200 x g for 3 min before resuspending and reseeding in EBM Basal Medium supplemented with the EGM Singlequots Supplement Pack ...
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bioRxiv - Bioengineering 2024Quote: ... 2.5×105-1×106 CD34+ cells were resuspended in P3 buffer (Lonza, Basel, Switzerland, cat.: V4XP-3032) with complexed RNPs and electroporated using the Lonza 4D Nucleofector and 4D-Nucleofector X Unit (program DZ-100) ...
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bioRxiv - Developmental Biology 2023Quote: ... hbNES cells were nucleofected using the Amaxa 4D-Nucleofector X-unit system (Lonza Bioscience, cat# AAF-1002X) when they had grown to 75% confluency ...
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bioRxiv - Biophysics 2023Quote: the first cell lineage (named ASMC_1 onwards) is a commercial immortalized human ASMCs lineage purchased from Lonza and delivered at passage 3 ...
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bioRxiv - Cell Biology 2023Quote: The following hTERT RPE-1 cells were all tested free of mycoplasma contamination using Myco Alert (Lonza) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 × 105 to 2 × 105 cells were collected and resuspended in 15 µL of nucleofection buffer (Lonza). For each reaction ...
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bioRxiv - Microbiology 2023Quote: ... Monolayers of CHSE-214 cells were cultured until 90-100% confluent and detached by Trypsin EDTA (Lonza). 4 transfection reactions ...
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bioRxiv - Immunology 2022Quote: ... 24 and 48 well-plates in EGM™-2 Endothelial Cell Growth Medium-2 BulletKit™ (LONZA) supplemented with GlutaMax (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: RNP electroporation with 4D-Nucleofector™ were performed according to 4D-Nucleofector™ Protocol C2C12 cells (Lonza). Freshly thawed C2C12 cells were recovered and subculture 2 days before Nucleofection ...
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bioRxiv - Cancer Biology 2023Quote: 1 x 103 cells per dish were plated onto 35mm dishes in 0.4% NuSieve Agarose (Lonza #50081). Six replicates of each condition were plated ...
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bioRxiv - Cell Biology 2023Quote: ... differentiated HepaRG hepatocyte-like cells (HPR116080, Biopredic, Lot HPR116NS080003 and HPR116239-TA08 or NSHPRG, Lonza, Lot HNS1014) were pre-cultured as previously described with a modification to medium composition9 ...
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bioRxiv - Immunology 2023Quote: ... cells were transfected with pcDNA3.1 encoding full-length mouse PD-1 or its mutant using nucleofection (Lonza). Transfected cells were sorted for uniform PD-1 staining and culture in medium with 0.4 cells and reporter Jurkat cells stably expressing WT or MT chimeric PD-1 (mhPD-1 ...
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bioRxiv - Cancer Biology 2023Quote: ... LNCaP-ABL and LNAI cells were cultured in RPMI 1640 with L-glutamine (Lonza, Switzerland #12-702Q) supplemented with 10% charcoal-stripped fetal bovine serum (Biowest ...
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An indocyanine green-based liquid biopsy test for circulating tumor cells for pediatric liver cancerbioRxiv - Cancer Biology 2023Quote: ... All widely available cell lines were grown in Eagle’s Minimum Essential Medium (EMEM, Lonza, Allendale, NJ, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: Human breast adipose tissue was incubated in mammary epithelial cell growth base medium (MEBM) (Lonza #cc-3151) supplemented with 0.5% bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2023Quote: ... and the Mel JuSo and UACC-257 melanoma cell lines were cultured in DMEM (Lonza, Basel, Switzerland) supplemented with 10% FBS (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2023Quote: ... siRNAs (1 μg per ml) were nucleofected into ISE6 cells using the 4D-Nucleofector System (Lonza Bioscience). Tick cells were centrifuged at 100 x g for 10 minutes to pellet the cells ...
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bioRxiv - Molecular Biology 2023Quote: One million Aire wild-type mTEC 3.10 cells were suspended in 100 μL of nucleofector solution (Lonza) containing 20 pmol of spCas9-GFP ...
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bioRxiv - Microbiology 2023Quote: ... Tufts University) and Vero (ATCC CCL-81) cells were grown in Dulbecco’s modified Eagle medium (DMEM, Lonza) containing high glucose and sodium pyruvate and supplemented with L-glutamine ...
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bioRxiv - Cell Biology 2023Quote: ... Both ODMCs and VSMCs were cultured on 1% gelatin coated cell culture plates in SMGM2 medium (Lonza) at 5% CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... ∼2-4 million cells were electroporated using the T020 method of the Nucleofector™ 2b device (Lonza). Immediately after electroporation ...
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bioRxiv - Genomics 2024Quote: ... synthesized crRNA:tracrRNA duplex (IDT) were transfected into 1.0E5-2.0E5 cells using the 4D nucleofector X unit (Lonza), with or without pre-incubation with equal molar Cas9/dCas9 protein (IDT 1081058,1081066) ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T and CHO-K1 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Lonza, Walkersville, MD, USA) supplemented with 2 mM L-glutamine (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Transfection of U266 cells was performed as described previously [49] using the Nucleofector system (Lonza, VCA-1003). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... UWB1.289 (ATCC; CVCL_B079) cell lines were cultured in RPMI-1640 media containing Mammary Epithelial Growth Medium (Lonza) (50% v/v ...
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bioRxiv - Cell Biology 2024Quote: ... Four days after co-nucleofection in RKO cells (Nucleofector 2b device using program A-024 and Lonza Cell Line Nucleofector® Kit V #VCA-1003 ...
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bioRxiv - Bioengineering 2024Quote: ... and seeded with human pulmonary artery endothelial cells in rectangular channel experiments (HPAEC, Lonza, Fig.1A-D), or human umbilical vein endothelial cells in round channel experiments (HUVEC ...
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bioRxiv - Developmental Biology 2024Quote: ... For BMP stimulation cells were transferred into serum-free medium X-VIVO 10 (Lonza, Walkersville, Maryland, USA). After 24 h in the serum-free medium treatment with BMP ligands was performed for 48 h as described above for HSPCs.
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bioRxiv - Microbiology 2024Quote: Harvested basal cells were cultured in complete small airway epithelial growth media (SAGM) (Lonza, Cat. CC-3118), with propagation for up to 10 passages ...
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bioRxiv - Cell Biology 2024Quote: ... HMVEC-L were cultured in EGMTM-2MV Microvascular Endothelial Cell Growth Medium-2 BulletKitTM (Lonza, CC-3202) at 37°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... CD34+ cells were resuspended in P3 buffer plus supplement (cat.: V4XP-3032; Lonza Bioscience, Walkersville, MD, USA) with complexed RNPs and electroporated using the Lonza 4D Nucleofector (program DZ-100) ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were tested biweekly during experiments using a mycoplasma detection kit to confirm absence of mycoplasma contamination using the Lonza MycoAlert® (Lonza #LT07-318). HCC1806 cells were grown in RPMI 1640 Medium (Life Technologies #11875093 ...
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bioRxiv - Neuroscience 2019Quote: ... Cortical neurons from C57BL/6J and C57BL/6J Sorcs1flox/flox mice were electroporated with DNA just before plating using an AMAXA Nucleofector kit (Lonza, Cat #VPG-1001). Cortical neurons from C57BL/6J Sorcs1flox/flox mice were transfected at DIV7 using Effectene (Qiagen ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were routinely authenticated using short tandem repeat profiling and verified to be free of mycoplasma using the MycoAlert mycoplasma detection kit (Lonza Cat# LT07-418), by the QIMR Berghofer Medical Research Institute analytical facility (Brisbane ...
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bioRxiv - Cancer Biology 2020Quote: ... A total of 1.5×106 tumor cells (td-tomato MDA-MB-231 SORE6>GFP) were transfected with 10 μl of 20 μM siRNA stock solution using Nucleofector Kit V from Lonza (cat# VCA-1003) for 48 h ...
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bioRxiv - Biophysics 2023Quote: ... we transfected HeLa cells with SNAP-tagged CENP-A (generous gift from Dan Foltz) in combination with either empty vector or GFP-CENP- C using the Amaxa Nucleofector kit R (Lonza Bioscience, Walkersville, MD) per instructions.
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bioRxiv - Cell Biology 2020Quote: ... iPS cells were then nucleofected with the Amaxa Nucleofection system using the following program (P3, CA-137) (Lonza). The cells were allowed to recover for 36-48 hours at high density in the presence of ROCK inhibitor and then isolated by FACS for GFPhi cells ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant plasmids were verified by Sanger sequencing and electroporated into E14 cells using the Nucleofector II platform (Lonza) (program A-013) ...
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bioRxiv - Immunology 2022Quote: ... Thawed PBMCS were rested overnight at 37 °C in X-VIVO 15 Serum-free Hematopoietic Cell Medium (Lonza) supplemented with 5% human Ab serum ...
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bioRxiv - Molecular Biology 2021Quote: Telomerase human aortic endothelial cells (TeloHAECs) (ATCC, CRL-4052) were cultured in EBM-2 basal media (Lonza, 3156) supplemented with EGM-2 Bullet kit (Lonza ...
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bioRxiv - Neuroscience 2021Quote: ... siRNA molecules were electroporated into isolated postnatal cerebellar granule cells using the Nucleofector 2b Device (Lonza, AAB-1001) as previously described (Gartner et al. ...
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bioRxiv - Immunology 2021Quote: ... and the next day (day 1) sequentially seeding primary human lung microvascular endothelial cells (Lonza, CC-2527, P5) and primary human lung alveolar epithelial cells (Cell Biologics ...
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bioRxiv - Molecular Biology 2019Quote: ... a modified antisense or control oligonucleotide (1 μM) was transfected into 3 × 106 cells using Nucleofector technology (Lonza) 16–18 h before the assay ...
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bioRxiv - Immunology 2021Quote: ... Approximately 15,000 HMEC-1 cells/100 μl were seeded on coated wells using EGM-2 media (Lonza, Switzerland) and incubated overnight either with conditioned media from toxin-treated iPSC-MSC or vehicle at 37 °C ...