Labshake search
Citations for Lonza :
4201 - 4250 of 4910 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... RNA complex and Alt-R HDR Donor Oligos were transfected into HCT116 cells by electroporation (Lonza Nucleofector 96-well Shuttle System ...
-
bioRxiv - Cancer Biology 2024Quote: ... cell pellets were resuspended in two to three volumes of ACK lysis buffer (Lonza: #10-548E) according to the manufacturers’ specifications ...
-
bioRxiv - Bioengineering 2024Quote: ... which directly correlates to cellular metabolic activity.58 LSEC cells were maintained in EBM-2 (Lonza) media with EGM-2 BulletKit (Lonza) ...
-
bioRxiv - Molecular Biology 2020Quote: ... 4×106 cells were electroporated with 10 μg DNA of pBluescript II SK(+) carrying a human U6 promoter and EGFP-sgRNA using Nucleofector® V Kit (Lonza, Switzerland) as described above.
-
bioRxiv - Neuroscience 2019Quote: ... C57BL/6J: L315 control construct or L315 Nrxn triple knockdown construct) just before plating using AMAXA Nucleofector kit (Lonza, Cat #VPG-1001) and plated at 2500/cm2 on micro islands of mouse (CD-1 ...
-
bioRxiv - Cell Biology 2021Quote: ... OC activity was measured by releasing the europium conjugated human Type I collagen-coated on the bottom of the OsteoLyse™ Assay Kit (Lonza Biosciences) at each time point ...
-
bioRxiv - Cell Biology 2020Quote: ... These plasmids were transfected into NHDFs and 7SPs by electroporation using the Amaxa nucleofector kit and nucleofector 2b (Lonza, Walkersvill, MD, USA). Two days later ...
-
bioRxiv - Cancer Biology 2021Quote: ... All human cell lines were authenticated by Short Tandem Repeat analysis and tested for Mycoplasma contamination with a MycoAlert Mycoplasma Detection Kit (Lonza, Basel, Switzerland).
-
bioRxiv - Molecular Biology 2022Quote: ... Cell lines were cultures for no more than 30 passages and routinely tested for mycoplasma contamination using MycoAlert Mycoplasma Detection Kit (Lonza, Slough, UK).
-
bioRxiv - Microbiology 2020Quote: ... cell lines were authenticated using STR profiling (Eurofins Genomics) and monitored for mycoplasma contamination using the MycoAlert mycoplasma detection kit (Lonza Rockland, USA). Cells were cultured at 37 °C and 5 % CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM ...
-
bioRxiv - Cell Biology 2022Quote: ... Cell lines are cultivated at 37°C with 5% CO2 and were tested every 3 months for mycoplasma contamination using Universal Mycoplasma detection kit (ATCC, 30-1012K) or MycoAlert Plus Mycoplasma Detection Ki (Lonza, LT07-701). Cell lines were used no more than 30 passages.
-
bioRxiv - Cancer Biology 2019Quote: ... 2×105 cells were co-transfected with 2ug of the Cas9/sgRNA vector PxHF1* and 4 ul of ssODN HDR template (20 uM) using a Lonza X-Unit Nucleofector with P3 buffer kit (Lonza #V4XP-3032). Four days following transfection ...
-
bioRxiv - Microbiology 2019Quote: ... Fast-growing clonal cell lines confluent after three weeks of growth were expanded and confirmed to be mycoplasma-negative using the Myco-Alert PLUS kit (Lonza, Basel, Switzerland) prior to freezing for long-term storage in liquid nitrogen ...
-
bioRxiv - Molecular Biology 2020Quote: pSBbi-Pur-ABCC1 or empty vector were cotransfected with SB100X using the same Amaxa Nucleofector II and kit V (Lonza Group AG). Stably expressing cells were selected for using 10ug/mL puromycin (Gibco) ...
-
bioRxiv - Biochemistry 2022Quote: Normal bronchial epithelial cells (BEAS-2B / ATCC-CRL-9609) were cultured in collagen coated flasks using the bronchial epithelial growth medium supplemented with Lonza Bullet Kit (BEGM, Lonza CC-3170), as described by ATCC culture instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... HEK293T cells used in this study were confirmed to be negative for Mycoplasma Contamination and routinely tested using the MycoAlertTM Mycoplasma Detection Kit (Lonza, LT07-418). For experiments involving the SMAD4 gene ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... and the cell pellet was resuspended in Keratinocyte Growth Medium-2 Bullet Kit (KGM2 medium) consisting of KGM-2 Basal Medium and KGM-2 SingleQuots Supplements (Lonza, Basel, Switzerland), and then seeded onto Petri dishes coated with collagen IV ...
-
bioRxiv - Molecular Biology 2024Quote: ... The culture medium or the DNA harvested from the cultures were tested for mycoplasma contamination using a MycoAlert Mycoplasma Detection Kit (Lonza, LT07-418) or e-Myco PLUS PCR Detection Kit (BOCA scientific ...
-
Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... All cell lines were tested and negative for mycoplasma contamination by staff at the Mycoplasma Testing Facility (UNSW Sydney, Sydney, Australia) using the MycoAlertTM Mycoplasma Detection Kit (Lonza, Basel, Switzerland) every three months.
-
bioRxiv - Cancer Biology 2024Quote: ... Cell lines were validated by sequencing to contain KRAS and TP53 mutations and verified as Mycoplasma negative (MycoAlert Mycoplasma Detection Kit, Lonza, LT07-701). All cells were maintained in monolayer culture at 37°C and 5% CO2 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cell lines were authenticated by STR testing upon receipt and were routinely tested and verified as mycoplasma negative using the MycoAlert kit (Lonza, Cambridge, MA). Human recombinant TNF was purchased from BioLegend Inc (San Diego ...
-
bioRxiv - Immunology 2022Quote: LPS concentration in serum was determined using a chromogenic assay based on a Limulus amebocyte extract (LAL kit-terminal QCL1000) (LONZA, Basel, Switzerland). Samples were collected at the time of euthanasia via cardiac puncture to try to minimize as much as possible the chance of contamination ...
-
bioRxiv - Genomics 2023Quote: ... was mixed with 16.4 μL Nucleofector SolutionTM and 3.6 μL Supplement and incubated at room temperature for about 10 min according to the instruction of Amaxa 4D-Nucleofector X Kit TM (Lonza, #V4XP-3032). HepG2 ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were regularly passaged 2-3 times a week and routinely tested for mycoplasma contamination using MycoAlert Plus mycoplasma Detection kit (Lonza, #LT07-710).
-
bioRxiv - Cancer Biology 2023Quote: ... All cell lines were authenticated by STR profiling (Eurofins, Val Fleuri, Luxembourg) and were tested negative for mycoplasma (MycoAlert PLUS mycoplasma detection kit, Lonza, Basel, Switzerland).
-
bioRxiv - Cancer Biology 2024Quote: ... Cell lines were authenticated by short tandem repeat analysis and routinely tested for mycoplasma using the MycoAlert mycoplasma detection kit (Lonza, #LT07-710).
-
bioRxiv - Cancer Biology 2024Quote: ... after thaw for about 1 week and tested for mycoplasma regularly using the MycoAlertTM PLUS Mycoplasma Detection Kit (Lonza, CAT#: LT07-318). Cells were always used within 10 passages of authentication via STR analysis with CellCheck9 by IDEXX laboratories.
-
bioRxiv - Neuroscience 2024Quote: ... following a previously described protocol.43-44 Cultures underwent testing every other month using the MycoAlert Mycoplasma Detection Kit (Lonza LT07-318) and consistently tested negative for mycoplasma throughout the study.
-
bioRxiv - Cell Biology 2024Quote: ... the Ribonucleoprotein (RNP) complexes (9:1 sgRNA to Cas9 ratio) were assembled in Nucleofector Solution plus Supplement (Lonza Amaxa HMEC Nucleofector Kit) to a total volume of 100µL ...
-
bioRxiv - Genetics 2021Quote: ... RNPs containing recombinant CRISPR/Cas9 and sgRNA were transfected to human cell lines using a Nucleofector (Lonza). Stable cell lines were generated by selection of hygrogmycin resistance ...
-
bioRxiv - Molecular Biology 2021Quote: ... counted and transfected using the Lonza™ P3 Primary Cell 4D-Nucleofector™ X (Lonza, V4XP-3024) and electroporation program CA137.
-
bioRxiv - Developmental Biology 2021Quote: ... Cells were cultured on laminin-coated tissue culture flasks in SKGM-2 medium (Lonza®, Valkersville, MD). Medium was replaced every 3 days ...
-
bioRxiv - Cancer Biology 2022Quote: ... where 1×106 4T1 cells in 50 µL of phosphate-buffered saline (PBS, Lonza, cat. 17-512F) were subcutaneously (s.c. ...
-
bioRxiv - Bioengineering 2022Quote: ... Endothelial Cell Growth Basal Medium-2 (EBM) and EGM-2 SingleQuots™ Supplements were obtained from Lonza. Fetal bovine serum (FBS) ...
-
bioRxiv - Cell Biology 2022Quote: Primary Aortic Smooth muscle cells from ATCC (PCS-100-012™) (AoSMC) were cultured with SmbM2 (Lonza) with 1x SmGMTM-2 SingleQuotsTM Supplement Pack (CC-4149).
-
bioRxiv - Immunology 2021Quote: ... were expanded in 75 cm2 tissue culture flasks using human endothelial cell growth medium (Lonza, CC-3202) until 70-80% confluent.
-
bioRxiv - Molecular Biology 2019Quote: ... 75 ng of these substrates were electroporated into 200,000 cells with 1 ug of pMAX-GFP (Lonza) using the Neon system (Invitrogen ...
-
bioRxiv - Bioengineering 2019Quote: ... The cells were transfected with purified linear pFSC005 using the Nucleofector® 2b electroporator (Lonza, Morristown, NJ), following the Lonza Amaxa® Cell Line Nucleofector® Kit V optimized transfection protocol for Jurkat Clone E6-1 cells ...
-
bioRxiv - Developmental Biology 2019Quote: ... The human Atoh8 expression plasmid [30] was transfected by electropulsing using the Cell Line Nucleofector System (Lonza) following standard procedures provided by the manufacturers.
-
bioRxiv - Systems Biology 2019Quote: ... These adherent cells are grown as monolayer in Dulbecco’s modified Eagle’s medium (DMEM; Lonza, Levallois-Perret, France) supplemented with 10% (v/v ...
-
bioRxiv - Systems Biology 2021Quote: For fluorescent imaging, HEK293A cells (ATCC, USA) were maintained in Dulbecco’s Modified Eagle Medium (DMEM - Lonza, Switzerland) complemented with 10% fetal bovine serum (Biosera ...
-
bioRxiv - Bioengineering 2021Quote: Human umbilical vein endothelial cells (ECs) were cultured in endothelial growth medium (EGM-2; Lonza, Basel, Switzerland) supplemented with 1% penicillin-streptomycin-fungizone (Gibco ...
-
bioRxiv - Bioengineering 2020Quote: ... Human mesenchymal stem cells (MSCs, bone marrow-derived) and human lung fibroblasts (HLFs) were purchased from Lonza and cultured in DMEM (low glucose ...
-
bioRxiv - Cancer Biology 2020Quote: SEM cells (ACC-546, DSMZ) and MOLM13 (ACC-554, DSMZ) were maintained in RPMI-1640 medium (Lonza) containing 10% fetal bovine serum (FBS ...
-
bioRxiv - Cancer Biology 2020Quote: UWB1.289 and UWB1.289+BRCA1 cells were purchased from ATCC and were maintained in 1:1 MEBM (Lonza): RPMI 1640 with GlutaMAX (Thermo Fisher ...
-
bioRxiv - Cancer Biology 2020Quote: ... Smarca4f/f mouse lines) or 20,000 AALE cells were plated on a 0.35% low melting agarose (Lonza) placed over a 0.7% agarose layer in a 6-well plate ...
-
bioRxiv - Genetics 2020Quote: ... and cells were resuspended in 20µL/reaction of room-temperature Lonza nucleofection buffer P2 (Lonza V4XP-2024). The cell suspension was gently mixed with 2.5µL/reaction of appropriate RNP and then pipetted into a 96-well-format nucleofection cuvette for the Lonza 4D X unit or Shuttle unit (Lonza) ...
-
bioRxiv - Cell Biology 2019Quote: ... All cell lines used and generated in this study were routinely tested for mycoplasma contamination (MycoAlert, Lonza).
-
bioRxiv - Microbiology 2021Quote: Each transfection reaction was prepared by adding 2 µl of “primed” cells resuspended in SG buffer (Lonza) to a mixture of ...
-
bioRxiv - Microbiology 2020Quote: ... after which cells were washed twice with PBS and maintained in Eagle’s minimal essential medium (EMEM; Lonza) with 2% FCS ...