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Citations for Lonza :
3451 - 3500 of 4130 citations for Cytomegalovirus Cell Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 10×106 CD8+ T cells were resuspended in 250 µL of RT red phenol-free serum-free TheraPEAKTM X-VIVOTM-15 (BEBP02-061Q, Lonza). 10 µg of RNA coding for the α and β chains of the TCR recognizing the S7C epitope of NY-ESO-1 presented on HLA-A*02:01 (NY-ESO (157-165) ...
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bioRxiv - Genomics 2020Quote: 5 × 105 THP-1 cells were resuspended in 20µL nucleofection solution (16.4µL SG nucleofector solution + 3.6µL supplement 1) from the SG Cell Line 4D-Nucleofector™ X kit (Lonza, V4XC-3032). 300pmol PPP2R1A sgRNA (Synthego ...
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bioRxiv - Molecular Biology 2019Quote: ... POLE3 and POLE4 gene knockouts were generated in RPE1-hTERT Flag-Cas9 TP53-/- cells by electroporation of LentiGuide-Puro or LentiGuide-NLS-GFP vectors using an Amaxa II Nucleofector (Lonza). C16orf72 gene knockout clones were generated in RPE1-hTERT Flag-Cas9 TP53-/- cells by transfecting sgRNA/Cas9 ribonucleoprotein complex using Lipofectamine CRISPRMAX Cas9 Transfection Reagent (Invitrogen) ...
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bioRxiv - Genetics 2020Quote: ... All nucleofections were performed using 106 freshly-purified CD4 T cells in 100 μl nucleofection buffer using a Nucleofector 2b device (Lonza), based on previous optimisation studies (data not shown) ...
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bioRxiv - Developmental Biology 2019Quote: ... U2OS cells were transfected with Cas9 and guide RNA expression plasmids using Amaxa electroporation as recommended by the manufacturer (Lonza). Cas9 expression plasmid was from the Church lab (Addgene 41815) ...
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bioRxiv - Biochemistry 2019Quote: ... 3×107 cells were transfected with 10 μg of NotI linearized plasmids using the AMAXA electroporator (Lonza, program X-001) as described [14] ...
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bioRxiv - Molecular Biology 2019Quote: ... was modified by adding T2A-GFP at the C-terminal end and electroporated with the sgRNA vector into JB1 and BJ1 hybrid ES cells using the Amaxa nucleofector procedure (Lonza). 24 h post-electroporation ...
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bioRxiv - Cancer Biology 2020Quote: Bioluminescent detection of cellular ATP as a measure of cell viability was undertaken using ViaLight® Plus Kit (Lonza Inc.) reagents ...
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bioRxiv - Genetics 2020Quote: ... ∼1×106 cells were transfected with pairs of 5 µg gRNA plasmid and 4 µg ssODN (Supplementary Table 3) using AmaxaTM Human CD34 Cell NucleofectorTM Kit (Lonza) in the 2B-NucleofectorTM on the U-08 setting ...
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bioRxiv - Genetics 2020Quote: ... Primary neonatal Human Umbilical Vein Endothelial Cells (HUVEC) were sourced from three suppliers to provide genetic diversity (Lonza, Gibco, PromoCell). HUVECs were expanded in endothelial cell growth medium (Sigma ...
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bioRxiv - Cell Biology 2019Quote: Whole blood (50 μL) was obtained by retro-orbital bleed and red blood cells were lysed in ACK lysing buffer (Lonza). The remaining cells were stained with Live Dead Aqua (Life Technologies ...
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bioRxiv - Cancer Biology 2019Quote: ... Human cerebral microvascular endothelial cells (hCMEC/D3 [hCMEC]; gift from Dr. B. Weksler, Cornell Medical College) were grown in endothelial basal medium-2 (Lonza) supplemented with 5% FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... A20 cells were grown in RPMI 1640 medium (whereas all other cells were grown in DMEM) supplemented with 10% (v/v) FBS (Lonza) and with Pen/Strep Amphotericin B (Lonza ...
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bioRxiv - Cell Biology 2019Quote: ... DLD1 BRCA2-/- cells stably expressing full-length GFPMBP-BRCA2 and the variants (S206C and T207A) were transfected using AMAXA technology (Lonza) nucleofector kit V (Cat ...
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bioRxiv - Cell Biology 2019Quote: Human lung microvascular endothelial cells (HLMVECs) and Endothelial growth media-2 (EGM-2) were purchased from Lonza (Walkersville, MD, USA). Lung endothelial cells (ECs ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lines were obtained from the Berkeley Cell Culture Facility and were verified mycoplasma free with MycoAlert Mycoplasma Detection Kit (Lonza).
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bioRxiv - Neuroscience 2019Quote: ... the neurons were resuspended in DMEM/F12 and electroporated using a P3 Primary Cell 4D-Nucleofector X Kit L (cat. no. V4XP-3012, Lonza) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: 1-4 × 106 dissociated cells were re-suspended in 100 µL of Amaxa Mouse NSC Nucleofector Solution (VPG-1004, Lonza) with 5 µg of plasmid DNA ...
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bioRxiv - Bioengineering 2019Quote: ... RFP-labelled human lung microvascular endothelial cells (VeraVec, Angiocrine Biosciences Inc., San Diego, CA, USA) were cultured in EGM2 medium (Lonza) supplemented with growth factors according to the manufacturer’s instructions (EGM2-MV BulletKit ...
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bioRxiv - Bioengineering 2021Quote: ... Each chamber was connected to a reservoir that was pre-filled with 15 mL of warm serum-free EGM-2 (endothelial cell growth medium-2 without serum added from the kit, Lonza). ECFCs were harvested and resuspended in serum-free EGM-2 and added to the reservoirs to reach an overall cell density of 125,000 cells/mL ...
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bioRxiv - Immunology 2019Quote: The following chemically defined or partially defined serum-free media were used: X-VivoTM15 Hematopoietic Cell Medium (Lonza; Bend, OR); Macrophage SFM (Thermo Fisher ...
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bioRxiv - Genomics 2020Quote: ... 5 million cells were transfected with 5 μg of DNA FAIRE-STARR library plasmid using the Amaxa Nucleofector kit V (Lonza). For each condition ...
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bioRxiv - Systems Biology 2021Quote: ... and 2×106 iPSCs were mixed with pX459-sgRNA vector and ssDNA repair donor template and electroporated with Lonza 4D-Nucleofector system using the P3 Primary Cell Nucleofector Kit (Lonza). The iPSCs were then recovered in mTeSR1 medium for 48 hours before adding puromycin for 3-day selection ...
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bioRxiv - Biochemistry 2020Quote: ... COS7 cells were cultured in DMEM with 4.5g/l glucose supplemented with 10% DMEM and antibiotics (Lonza Biologics, Slough, UK). transiently transfected with expression plasmids using TransIT-2020 (Mirus ...
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bioRxiv - Bioengineering 2020Quote: ... Human umbilical vein endothelial cell (HUVEC, C25271) and endothelial growth medium (EGM-2MV, CC4147) were purchased from LONZA (Basel, Switzerland). Rabbit anti-perilipin antibody (9349S ...
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bioRxiv - Bioengineering 2021Quote: ... 200,000 cells were electroporated with 1000 ng adRNA plasmid or 48 pmol of IVT RNA using the Amaxa SF cell Line 4D-Nucleofector X kit (Lonza) as per the manufacturer’s instructions.
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bioRxiv - Bioengineering 2020Quote: ... The cells were used up to passage number 12 and were tested for mycoplasma contamination (MycoAlertTM Mycoplasma Detection Kit, Lonza) at first and last passage number ...
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bioRxiv - Biochemistry 2021Quote: ... Transfections of full-length human EGFR (wild-type or noted variants) into Ba/F3 or eHAP cells were performed by electroporation using a Nucleofector 2b device (Lonza) as described previously (Kiyatkin et al. ...
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bioRxiv - Biophysics 2021Quote: ... The cell suspension was transferred to an electroporation cuvette and electroporated using program X-001 on an Amaxa Nucleofector II (Lonza). Cells in the cuvette were mixed with 300 μl of DMEM ...
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bioRxiv - Neuroscience 2021Quote: ... The efficacy of siRNA targeting Kdm6a was assessed by electroporation of neurons using a 4D-Nucleofector X Unit and the corresponding P3 Primary Cell nucleofection kit (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... percent cell death was quantified by flow cytometry or cells were re-suspended in media and an equal volume of Trypan blue solution (Lonza) was added ...
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bioRxiv - Cancer Biology 2020Quote: ... Both cell types were cultured and used in TOC devices for up to seven passages in complete growth medium from Lonza (FGM-2 for NHLFs and EGM-2 for ECFC-ECs) ...
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bioRxiv - Biochemistry 2021Quote: ... Endothelial cells were cultured in EBMTM Plus Basal Medium supplied with EGMTM Plus SingleQuotsTM supplements (CC-5035; Lonza, Basel, Switzerland). All cell lines were maintained in a humidified incubator at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: 2×106 Elijah cells were washed in PBS and resuspended in 20 μl P3 Primary Cell Nucleofector Solution buffer prepared with Supplement 1 buffer (Lonza) according to the manufacturer’s instructions (P3 Primary Cell 4D-Nucleofector X Kit S) ...
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bioRxiv - Neuroscience 2020Quote: iPSNs were transfected using the Lonza nucleofection system (program DC104) and a Lonza P3 Primary Cell 4D Nucleofector Kit (Lonza). POM121 ...
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bioRxiv - Microbiology 2021Quote: ... Both resuspensions were mixed in a 100 μl cuvette (P3 Primary cells 4D-Nucleofector X kit L, V4XP-3024, Lonza). The programme FI-158 was used for electroporation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cell lysate containing tau seeds was combined with the Tau(P301S)-YFP cells at 1.8 µg/mL and electroporation was performed on an Amaxa Nucleofactor instrument (Lonza Bioscience) using the program #Q-001 ...
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bioRxiv - Systems Biology 2020Quote: ... 24 hours activated DC and T cells were incubated in 96 well plates at a DC/T ratio 1:5 in Xvivo15 medium (Lonza). After 6 days ...
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bioRxiv - Microbiology 2021Quote: ... Viral supernatants were collected 3 and 6 days post-infection and induced-cell death and viral titers were determined in the ToxiLight Bioassay (Lonza) and PFA ...
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bioRxiv - Microbiology 2020Quote: ... The same number of cells (2 x 106 cells) were lysed in 100 µl of 1% CHAPS/PBS and analyzed on 4-20% SDS-PAGE gel (Lonza). The envelope proteins were detected by 2F5 (specific for gp41 ...
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bioRxiv - Molecular Biology 2020Quote: ... at Day 3-4 of phase I culture were electroporated using P3 Primary Cell 4D NucleofectorTM X Kit (from Lonza) with program DZ100 (Bak et al. ...
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bioRxiv - Immunology 2021Quote: ... MICB or ULBP-1 genes after optimizing nucleofection conditions using primary cell 4D nucleofector kit and 4D nucleofector system (Lonza). After 48 hours of culture ...
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bioRxiv - Cancer Biology 2020Quote: ... All cells were confirmed to be mycoplasma-free by MycoAlert Mycoplasma Detection Kit (Lonza, Morristown, NJ USA; Cat #: LT07-118). Cells were steroid-starved in phenol red-free medium containing 10% charcoal stripped-FBS (5% CS-FBS for C4-2 cells ...
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bioRxiv - Cancer Biology 2020Quote: The colorectal carcinoma cell lines HT29 (ATCC, Manassas, VA, USA) and Colo205 (ATCC) were cultured in RPMI (Lonza, Basel, Switzerland) and in McCoy’s 5A (Lonza) ...
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bioRxiv - Cell Biology 2020Quote: ... aliquots of obtained BM aspirates were seeded into cell culture flasks containing endothelial basal media (EBM-2, Lonza, Cologne, Germany) supplemented with 10% human platelet lysate (PL ...
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bioRxiv - Cell Biology 2022Quote: ... were nucleofected with either PGK-mO-Dam or PGK-mO-Dam-Zfp266 plasmids using the P4 Primary Cell 4D-Nucleofector X Kit (Lonza). 5 replicates were performed in total ...
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bioRxiv - Bioengineering 2022Quote: ... were used in passage 2 to 6 and were cultivated in Endothelial Cell Growth Medium-2 (EBM-2 medium supplemented with EGM-2 SingleQuots supplements, Lonza).
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bioRxiv - Neuroscience 2022Quote: ... 3 x 106 NSCs were mixed with 5 µg of corresponding DNA with the Amaxa P4 Primary Cell 4D-Nucleofector X Kit S (Lonza) and pulse was delivered using the CA137 programme in a 4D-Nucleofector X Unit (Lonza) ...
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bioRxiv - Cell Biology 2022Quote: ... All cell lines were tested to be negative for mycoplasma every 2–3 months using the MycoAlert Mycoplasma Detection Kit (Lonza).
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bioRxiv - Cancer Biology 2022Quote: ... cells were transfected with 5μg of selected plasmid (control, empty vector (EV) or containing guide RNAs using Amaxa Cell Line Nucleofector kit (Lonza Bioscience) according to the manufacturer’s instructions ...