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Citations for Lonza :
3701 - 3750 of 4130 citations for Cytomegalovirus Cell Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... All sorted and parental cell lines were confirmed to be mycoplasma-negative in a MycoAlert PLUS assay (Lonza, LT07-710) performed by the Koch Institute High-Throughput Sciences Facility (Cambridge ...
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bioRxiv - Neuroscience 2023Quote: ... before being introduced to the KOLF2 hiPSCs through electroporation (P3 Primary Cell 4D-Nucleofector X Kit, Program CA-137, Lonza). The resulting clones were screened by multiplexed amplicon sequencing on an Illumina Mi-Seq platform with the MiSeq Reagent Kit V2 (Illumina) ...
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bioRxiv - Cancer Biology 2022Quote: ... An iCasp9-CD20/CD19-CAR transposon minicircle carrying CD20 scFv[17] and CD19 scFv[18] and a qPBase plasmid were delivered into activated T cells using 4D-Nucleofector device (Lonza) and Quantum Nufect Kit (GenomeFrontier Therapeutics ...
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bioRxiv - Bioengineering 2023Quote: ... were expanded in type I collagen precoated culture flasks in endothelial cell growth medium-2 (EGM-2) BulletKit medium (CC-3162, Lonza). Culture flasks were precoated with 50 μg ml−1 collagen I rat tail (354236 ...
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bioRxiv - Bioengineering 2022Quote: ... B and T cell electroporations were performed using the Amaxa™ 96-well Shuttle™ with the 4D Nucleofector (Lonza). HSPC electroporations were performed in MaxCyte ATx ...
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bioRxiv - Biochemistry 2023Quote: ... All cell lines were regularly monitored and tested negative for the presence of mycoplasma using a commercial biochemical test (MycoAlert, Lonza).
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bioRxiv - Cancer Biology 2022Quote: ... The cell lines were authenticated and regularly tested for Mycoplasma using the MycoAlert PLUS detection kit by Lonza (LT07-710).
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bioRxiv - Bioengineering 2023Quote: Approximately 0.25 × 106 U2OS.eGFP-PEST cells were nucleofected with 300 ng of Cas9 expression plasmid and 30 ng of eGFP-targeting gRNA expression plasmid using the SE Cell line 4D-Nucleofector X Kit (Lonza) and DN100 pulse program ...
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bioRxiv - Bioengineering 2023Quote: ... and 50 ng of AcrIIA4 expression or 300 ng of Cas9-P2A-CSD-AcrIIIA4 plasmid and 30 ng eGFP-targeting gRNA expression plasmid using the SE Cell line 4D-Nucleofector X Kit (Lonza) and DN100 pulse program ...
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bioRxiv - Cancer Biology 2022Quote: ... Kp27MYCER and B95-8) and adherent cell lines (HeLa, HEK293T) were grown in RPMI-1640 or DMEM basal media (Lonza) respectively supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2022Quote: ... the Raji cells were transduced with mcDNA-CD19-CAR by using preset program DS104 of 4D-Nucleofector™ system (Lonza). Electroporation was performed according to the manufacturer’s instruction ...
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bioRxiv - Genetics 2023Quote: ... Samples of supernatant media from cell culture experiments were analyzed monthly for the presence of mycoplasma using MycoAlert PLUS (Lonza).
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bioRxiv - Cell Biology 2023Quote: ... were delivered as a ribonucleoprotein complex with the DNA donor template using a Nucleofector 2b Device and the Human Stem Cell Nucleofector Kit 1 (Lonza) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The volume of blood was determined and red blood cells were removed using ammonium-chloride-potassium (ACK) lysis buffer (Lonza) prior to antibody staining for flow cytometry analysis.
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bioRxiv - Cell Biology 2023Quote: ... All cell lines were authenticated by Multiplex Cell Line Authentication (MCA) and were tested for mycoplasma by MycoAlert Mycoplasma Detection Kit (Lonza). U2OS cells were transfected with the plasmids using Lipofectamine 2000 and according to the manufacturer’s instructions (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were nucleofected with 4 µg of the sgRNA-containing plasmid individually following the Amaxa Mouse ES cell Nucleofector kit recommendations (VPH-1001, Lonza). Later ...
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bioRxiv - Systems Biology 2023Quote: ... The isolated CD14+ cells were cultured in Dulbecco′s Modified Eagle′s Medium (BE12-614F, DMEM, BioWhittaker, Lonza, Basel, Switzerland) supplemented with 10 % human AB+ serum (pooled from five healthy donors) ...
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bioRxiv - Cell Biology 2023Quote: ... The cell lines used in this study were routinely tested for mycoplasma contamination using MycoAlert Mycoplasma Detection Kit (#LT07-318, Lonza), and mycoplasma-negative cells were used ...
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bioRxiv - Cell Biology 2023Quote: ... hESCs were nucleofected with RNPs using the 4D-Nucleofector and P3 Primary Cell 4D-Nucleofector X Kit and program CB150 (Lonza). After 24□hours ...
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bioRxiv - Genomics 2023Quote: ... purified Percoll-enriched mature schizonts (∼20 μl packed volume) were suspended in 100 μl of P3 primary cell solution (Lonza) containing 60 μg of linearized repair plasmid DNA (repair plasmid 1 and 2 separately in two separate transfection experiments ...
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bioRxiv - Molecular Biology 2023Quote: ... 1×105 cells were grown in each well of 12 well culture plates in SkGM-2 BulletKit growth medium (Lonza) to >90% confluency under standard culture conditions of 37°C and 5% CO2 ...
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bioRxiv - Synthetic Biology 2023Quote: ... An array of 20µL droplets of cell-laden hydrogel solutions were pipetted between two 25x75mm glass slides that were thrice treated with GelSlick (Lonza) separated by 3D-printed 400μm insert ...
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bioRxiv - Neuroscience 2023Quote: ... and the ssODN (1 μl; stock 100 μM, IDT) in 20 μl of nucleofection buffer P3 (P3 Primary Cell NucleofectorTM Solution, Lonza) were nucleofected (program CA137 ...
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bioRxiv - Immunology 2023Quote: ... A total of 5×106 cells per transgene were washed with phosphate-buffered saline (PBS) and resuspended in 100µl NucleofectorTM Solution V (Lonza, Germany) containing the expression vector (Fig ...
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bioRxiv - Cell Biology 2023Quote: ... pPB plasmids and pCMV-mPBase(neo-) encoding piggyBac transposase were co-transfected into MDCK cells by electroporation with an Amaxa nucleofector (Lonza). Cells were selected with the medium containing the following antibiotics ...
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bioRxiv - Biophysics 2023Quote: ... Samples of supernatant media from cell culture experiments were analyzed monthly for the presence of mycoplasma using MycoAlert PLUS (Lonza).
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bioRxiv - Cancer Biology 2024Quote: ... Electroporation was performed with the Lonza SF Cell line 4D-Nucleofector X solution (#V4XC-2032) and the 4D-Nucleofector X Unit (Lonza) (#AAF-1003X ...
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bioRxiv - Plant Biology 2024Quote: ... The fixed cells were washed three times with the same buffer and then embedded in 2 % low-melting-temperature agarose (SeaPlaque, Lonza). The embedded samples were post-fixed with 4 % potassium permanganate at 4 °C for 2 h ...
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bioRxiv - Systems Biology 2024Quote: The reporter cell line for the PTGR screen was created by nucleofection of haploid AN3-12 mESCs with 500ng of the reporter construct and 10 µg of a Tol2 transposase encoding plasmid using the Mouse ES Cell Nucleofector Kit (Lonza) according to the manufacturer’s protocol using an Amaxa Nucleofector (Lonza) ...
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bioRxiv - Neuroscience 2024Quote: ... iPSC lines were validated by Array Comparative Genomic Hybridization assessment of karyotype and copy number variation (Cell Line Genetics) and confirmed to be sterile and pluripotent via MycoAlert testing (Lonza), Pluritest Array Analysis ...
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bioRxiv - Cancer Biology 2024Quote: CRISPR-mediated mutagenesis was carried out by transiently introducing CRISPR/Cas9 RNA-nucleoprotein complex into cultured NB4 cells with the 4D-Nucleofector system (Lonza). The sample preparation kit was Lonza SF Cell Line 4D-Nucleofector Kit S (Cat# V4XC-2024) ...
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bioRxiv - Neuroscience 2024Quote: ... Four wells of EBs per line were seeded in a 4-layer cell discs coated with growth factor-reduced matrigel in X-VIVO15 medium (Lonza) supplied with GlutaMAX (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... and cultured in 96-well culture plates (2×103 cells/well) with serum or EP-stimulated MDMs supernatants in X-VIVO 15 medium (Lonza) with 10 U/mL of IL-2 (PeproTech ...
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bioRxiv - Molecular Biology 2023Quote: ... A cell count ranging from 1 × 10^5 to 2 × 10^5 cells was collected and suspended in 15 µL of nucleofection buffer (Lonza). Electroporations were conducted in the strip format ...
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bioRxiv - Immunology 2023Quote: ... To this end T cells were washed once in PBS and resuspended in complete P3 nucleofection buffer (Lonza, #V4XP-3032). 1e6 cells were then mixed with either Cas9 or base editor mRNA (4 - 4.5 µg ...
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bioRxiv - Immunology 2023Quote: ... of target genes in primary human T cells were generated by Cas9 ribonucleoprotein (RNP) electroporation using a 4D Nucleofector (Lonza) and P3 primary cell nucleofection reagents (Lonza ...
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bioRxiv - Cancer Biology 2023Quote: For the multi-ome analysis in young versus aged human bone marrow, bone marrow cells from young donors (26-year-old female, and 24-year-old male) were purchased from Lonza, while bone marrow samples from aged donors (70 and 77-year-old females ...
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bioRxiv - Bioengineering 2023Quote: ... Human umbilical vein endothelial cells (HUVEC) (cAP-0001, Angio Proteomie, USA) were suspended in EGM2 medium (CC-3162, Lonza, Switzerland) at a density of 1–2 × 107 cells/ml ...
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bioRxiv - Biochemistry 2023Quote: ... 100pmol synthetic pegRNA (IDT) and 50pmol sgRNA (IDT) using the P3 primary cell nucleofector kit (cat#V4XP-3032, Lonza Biosciences) and program EH-115 on an Amaxa 4D-Nucleofector ...
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bioRxiv - Biochemistry 2023Quote: ... Sf21 cells seeded at 500,000 cells/mL were transfected with recombinant bacmid DNA to generate the initial baculovirus (P1) in Insect-XPRESSTM Protein-free Insect Cell Medium (Lonza). The P1 virus was amplified two times (P2 and P3 ...
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bioRxiv - Microbiology 2023Quote: ... Virus stocks were propagated in Vero E6 cells, viral stocks and tissue culture supernatants were checked for endotoxin (Johnson et al., 2005) and mycoplasma (MycoAlert, Lonza) (La Linn et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting cells were transduced with the STAT regulatory reporter (4M67-GFP) (Wei et al., 2014) overnight in suspension in complete Mammary Epithelial Cell Growth Media (MEGM+) (Lonza, cat ...
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bioRxiv - Cell Biology 2023Quote: WT cells were transfected with the sgRNA-containing plasmids by electroporation with a Nucleofector device (Lonza; in Mirus transfection solution). Cells were plated in culture dishes and maintained for three days ...
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bioRxiv - Immunology 2023Quote: Mature human 3D microvascular tissue models were grown by seeding human umbilical vein endothelial cells (HUVECs) (Lonza Cat# CC-2519) into a collagen/matrigel mixture as described82,91 ...
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bioRxiv - Molecular Biology 2023Quote: ... MRTF-WT and MRTF knockout in NIH 3T3 (MRTF-KO-1 and MRTF-KO-2) cells were cultured in DMEM (BE12-614Q, Lonza) supplemented with 10 % Fetal Bovine Serum (FBS ...
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bioRxiv - Bioengineering 2024Quote: ... and activated using anti-human CD3/CD28 dynabeads (Cell Therapy Systems, Thermo) at a 1:1 ratio in XVivo 15 media (Lonza) supplemented with 5% FBS (MilliporeSigma ...
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bioRxiv - Bioengineering 2024Quote: ... B cells were edited 3-5 days after isolation or thawing using the Lonza Nucleofector 4D (program EO-117) using 1×106 cells per well of a 16-well Nucleocuvette Strip (Lonza). Immediately following nucleofection ...
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bioRxiv - Immunology 2023Quote: ... The transfection was carried out by nucleofection using Amaxa® Cell Line Nucleofector Kit V (Program X-001, Lonza, USA). At 24 h after transfection ...
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bioRxiv - Neuroscience 2023Quote: ... we routinely checked cell cultures for the presence of mycoplasma using the MycoAlert™ Mycoplasma Detection Kit (#LT07-218, Lonza).
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bioRxiv - Microbiology 2023Quote: ... or 1×106 CEM-M7 Cas9 cells were transfected with the HiFi Cas9 Nuclease V3 (IDT)/gRNA complex (80 pmol/300 pmol) (Lonza) using a non-targeting or a GRN(5’-GCGATCCTGCTTCCAAAGATC-3’) ...