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Citations for Lonza :
3351 - 3400 of 4130 citations for Cytomegalovirus Cell Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... PBMC were seeded at 1.5×106 cells per cm2 in X-VIVO 10 media (Cat. No. BE04-743Q, Lonza) +1% penicillin-streptomycin (Cat ...
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bioRxiv - Microbiology 2022Quote: ... and neonatal dermal microvascular endothelial cells (hDMVEC) were maintained as monolayer cultures in EBM-2 medium (Lonza, Walkersville, USA) supplemented with 5% fetal bovine serum ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: NIH-3T3 cells were grown in complete NIH-3T3 media (DMEM with 4.5g/L Glucose and 4mM L-Glutamine (Lonza), 10% ...
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bioRxiv - Microbiology 2022Quote: ... NHBE cells from a single donor were amplified and differentiated into air-liquid interface cultures as recommended by Lonza and described in (49) ...
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bioRxiv - Neuroscience 2023Quote: ... The two RNP complexes were then combined and diluted in 20 μL of P3 Primary Cell Nucleofector Solution (Lonza). To electroporate the Cas9 RNP complexes into hiPS cells ...
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bioRxiv - Neuroscience 2022Quote: Nucleofections were done on cortical neurons using the P3 Primary Cell 4D-Nucleofector X Kit (Lonza, LZ-V4XP-3024), on the day of preparation and dissociation (DIV 0) ...
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bioRxiv - Microbiology 2022Quote: ... Cell pellets were carefully enrobed in an equal volume of molten 5% low-melting temperature agarose (Lonza, Rockland, ME) and allowed to cool ...
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bioRxiv - Systems Biology 2022Quote: DMECs were plated on poly-L-lysine-coated 6-well glass-bottom plates at 8,450 cells per cm2 in either BPM or growth medium (EGM2-MV, Lonza). After 24 hours ...
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bioRxiv - Immunology 2022Quote: ... 1 x 106 T-cells were pelleted and re-suspended in 100 μL nucleofection solution (Lonza, Walkersville, MD, USA). T-cell suspensions were mixed with 5 μl RNP complex and cell/RNP mix transferred to Nucleofector cuvette ...
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bioRxiv - Molecular Biology 2024Quote: ... once the cell confluency reached 90% the culture medium was changed to 10 mL XVIVO-10 medium (Lonza #(BE)BP04-743Q ...
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bioRxiv - Molecular Biology 2024Quote: ... 33 μL of cell suspension was mixed with 66 μL of solution 1 (0.83% low-melting-point agarose SeaPlaque GTG (Lonza), 170 mM sorbitol ...
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bioRxiv - Cancer Biology 2024Quote: ... and resuspended in 20 µL of SF Cell Line Nucleofector™ Solution with the supplement added (Lonza V4XC-2032). The cells and the RNP complex were carefully mixed and transferred to a well of the 16-well Nucleocuvette™ ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were nucleofected with the DT-130 program using the 4D-Nucleofector system with Core and X unit (Lonza). Nucleofected cells were incubated for 10min at room temperature before being transferred to a 6-well plate containing pre-warmed and equilibrated medium (DMEM + 10% FBS) ...
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bioRxiv - Molecular Biology 2024Quote: ... 33 μL cell suspension was mixed with 66 μL of solution 1 (0.83% low-melting-point agarose SeaPlaque GTG (Lonza), 170 mM sorbitol ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were screened every two months for mycoplasma contamination using the MycoAlert™ Mycoplasma Detection Kit (Lonza, Basel, Switzerland). Passaged cells were continually monitored for viability using Countess cell counting slides (Thermo-Fisher ...
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bioRxiv - Bioengineering 2024Quote: ... Human primary bone marrow MSCs derived by plastic adherence of mononucleated cells from human bone marrow aspirate donors (Lonza) and were cultured in α-minimal essential medium (αMEM ...
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bioRxiv - Microbiology 2024Quote: ... Genetica Cell Line Testing) and cultures were regularly tested for mycoplasma contamination using the MycoAlert mycoplasma detection kit (Lonza). HEK293T and Calu-3 (ATCC-HTB-55 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lines were regularly authenticated by STR profiling (LabCorp, Burlington, NC) and tested for mycoplasma (MycoAlert, Lonza #LT07-218). To create a chronic CuHiexposure model ...
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bioRxiv - Cancer Biology 2024Quote: ... Red blood cell lysis was performed for 5 min at room temperature in ACK lysing buffer (Lonza, #10-548E). For flow cytometry ...
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bioRxiv - Microbiology 2024Quote: ... Cell pellets were carefully enrobed in an equal volume of molten 5% low-melting temperature agarose (Lonza, Rockland, ME) and allowed to cool ...
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bioRxiv - Neuroscience 2023Quote: ... We regularly monitored all cell cultures for mycoplasma contamination using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-218).
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bioRxiv - Biochemistry 2023Quote: Plasma cholesterol was depleted by treating HEK293 cells with 5 mM MβCD for 30 min in Pro293A-CDM (Lonza); this short period of MβCD treatment was deemed sufficient to removed 50% of endogenous cholesterol from cells (34) ...
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bioRxiv - Cell Biology 2024Quote: ... All cell lines were tested for mycoplasma at each batch freezing by PCR (32) and biochemical assay (MycoAlert, Lonza).
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bioRxiv - Cell Biology 2023Quote: ... after which the mixture was transferred to an electroporation cuvette and cells were electroporated in a 2b-Nucleofector (Lonza). Finally ...
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bioRxiv - Developmental Biology 2024Quote: ... H9 cells were transfected with the PB-GFP-P2A-ΔNβCAT plasmid and a Piggybac transposase-coding plasmid using the P3 primary cell 4D nucleofector kit (Lonza). After 48 hours ...
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bioRxiv - Immunology 2024Quote: ... All cell lines were tested negative for mycoplasma contamination using the Mycoalert™ Mycoplasma Detection Kit (Lonza Bioscience Solutions) prior to use in functional assays.
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bioRxiv - Biochemistry 2024Quote: Human HeLa cells (ATCC) were grown at 37°C in 5% CO2 using Dulbecco’s Modified Eagle Medium (Lonza, USA) supplemented with 10% FBS superior (Biochrom ...
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bioRxiv - Bioengineering 2024Quote: Commercially available primary adult human mesenchymal stem cells (hMSCs) from Donor 47506 were used (Lonza Group Ltd., Basel, Switzerland). Cells were cultured at passage 3 and seeded in triplicate in hMSC Growth Medium (MSCGM™ Mesenchymal Stem Cell Growth Medium ...
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bioRxiv - Bioengineering 2024Quote: hMSC were plated at 15,000 cells/well in 24-well plates and grown overnight in a growth medium containing 10% FBS (Lonza). When cells reach 80% confluency ...
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bioRxiv - Cancer Biology 2024Quote: ... Human Umbilical Vein Endothelial Cells (HUVECs) were cultured in complete EGM-2 (cat# CC-3162, Lonza, Allendale, NJ, USA) and were not used beyond passage five for any experiments.
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bioRxiv - Bioengineering 2024Quote: hMSC were plated at 15,000 cells/well in 24-well plates and grown overnight in a growth medium containing 10% FBS (Lonza). Once cells reached 80% confluency ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid transfections were performed using the 4D-Nucleofector and the SE cell line 4D-Nucleofector X S kit (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... different tumor cell lines were stained with carboxyfluorescein succinimidyl ester (CFSE) following the manufacture’s protocols and cultured in X-vivo (Lonza) supplemented with 5% FBS (Gbico ...
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bioRxiv - Bioengineering 2023Quote: ... gRNAs were complexed with recombinant Cas9 (IDT Cat#1081059) and introduced into cells by electroporation (4D-Nucleofector, Lonza Biosciences) according to manufacturer protocols ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 µM electroporation enhancer (IDT)) into 2 x 105 KPC cells on a Nucleofector 4D (Lonza Biosciences, Walkersville, MD) with an SF kit (Catalog ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 x 105 cells were transfected with the pU6-Sp-pegRNA-HEK3-insCTT plasmid (1,000 ng) using the SF Cell Line 96-well Nucleofector Kit (Lonza). Program code was FF-120.
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bioRxiv - Genomics 2023Quote: 200,000 PUER cells were transfected with 500ng each of Cas9 and donor vector in SF buffer (Lonza, V4SC-2096) using program CM134 of the 4D-Nucleofector (Lonza) ...
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bioRxiv - Bioengineering 2023Quote: ... single guide hybrids were mixed with 3uM Cas9 nuclease (Berkeley Labs) at a 1.2:1 ratio and delivered to cells by Lonza 3D (CA-137 ...
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bioRxiv - Biochemistry 2023Quote: ... DNA (10 μg) was transfected into SmOx P9 cells using a 4D nucleofector system (Lonza Group AG, Basel, Switzerland) with program FI-115 ...
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bioRxiv - Cancer Biology 2023Quote: ... hTERT ipn02.3 2λ cells were transfected by electroporation using the Amaxa Basic Nucleofector Kit for Primary Mammalian Fibroblasts (Lonza) and the program U-023 ...
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bioRxiv - Bioengineering 2023Quote: ... each device was seeded directly on the PREDICT96-ALI membrane in the apical chamber with 10,000 cells in Small Airway Epithelial Growth Medium (Lonza) containing 100 U/mL penicillin–streptomycin (Thermo Fisher) ...
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bioRxiv - Neuroscience 2023Quote: Electroporation with freshly assembled RNP complex was performed on hiPSCs using the Human Stem Cell Nucleofector Kit 1 (Lonza) in conjunction with the Amaxa Nucleofector 2b system (Lonza ...
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bioRxiv - Immunology 2023Quote: moDCs were cultured with allogenic naïve CD4+ T cells in a 1:10 ratio in complete IMDM (IMDM (BE12- 722F, Lonza) with 100 U/mL penicillin ...
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bioRxiv - Cancer Biology 2023Quote: ... cells (used for TT-TL seq and ChIP-seq spike-ins) were cultured in Schneider’s modified Drosophila medium (Lonza) supplemented with 10% FBS at 27°C without CO2 ...
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bioRxiv - Microbiology 2023Quote: ... All cell lines used in this study were regularly screened for Mycoplasma contamination with the MycoAlert Detection Kit (Lonza).
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bioRxiv - Cancer Biology 2023Quote: SK-N-BE (2) (ATCC: no. CRL-2271) cells were cultured in Dulbecco’s Modified Eagle Medium (Lonza, Cat# BW12614F), supplemented with heat-inactivated FBS (Hyclone ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×10E6 ME-1 cells were nucleofected with CRISPR/Cas9 plasmids (2 μg each) using Nucleofector Technology (Lonza Biologics) with the program X-01 and Amaxa Cell Line Nucleofector Kit V ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were pelleted at 1000 rpm for 5 min and resuspended in 100 μL nucleofector solution (Lonza, #VPB-1002) before adding 2.5 μg of plasmid ...
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bioRxiv - Cancer Biology 2022Quote: ... The B16 and the human melanoma cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Lonza; BE12-604F/U1) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: Normal human bronchial epithelial cells (HBECs) from 3 independent donors (Supplemental Table S1) were obtained from Lonza (CC-2540) and cultured in BEGM growth media (Lonza CC-3170) ...