Labshake search
Citations for Lonza :
3251 - 3300 of 4130 citations for Cytomegalovirus Cell Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2019Quote: ... Hela cells were electroporated with plasmids using Ingenio electroporation kit (SopaChem) and the Nucleofector™ II/2b device (Lonza). Cells were harvested 24h post-electroporation ...
-
bioRxiv - Genetics 2020Quote: ... 5μg vector (in 5μl) transfected into 5 million CD4 T cells in 100μl 1M nucleofection solution67) using a Nucleofector 2b device (Lonza; program V024 for resting T cells and T023 for stimulated T cells) ...
-
bioRxiv - Molecular Biology 2019Quote: RPE-1 cells were transfected with 20 pmol siRNA by nucleofection (Amaxa Lonza 4D program EA-104 P3 solution) or Lipofectamine RNAiMAX transfection reagent (ThermoFisher ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... CT26wt and C51wt cells were transfected with the A33 gene in pcDNA3.1(+) using the Amaxa™ 4D-Nucleofector (Lonza) and the SG Cell Line 4D-Nucleofector® X Kit L (Lonza ...
-
bioRxiv - Genomics 2020Quote: ... 1 million U2OS cells were transfected using the Nucleofector 2b with 2 μg Plasmid DNA using kit V (Lonza) according the manufacturer’s instructions.
-
bioRxiv - Developmental Biology 2021Quote: ... pPBCAG-hph and a PiggyBac Transposase vector using the AMAXA Mouse Embryonic Stem Cell Nucleofector Kit (LONZA, VPH-1001). Transfected cells were selected with 200 μg/ml hygromycin B Gold (Ibian tech. ...
-
bioRxiv - Developmental Biology 2021Quote: ... 5×106 EL16.7 TST ESCs were nucleofected with the AMAXA Mouse Embryonic Stem Cell Nucleofector Kit (LONZA, VPH-1001) using program A-30 with 1.6 µg each of GFP and tdTomato circularised targeting vectors and 5 µg single gRNA vector PX459 (5’-TATACCTAATCATTATGCCG-3’ ...
-
bioRxiv - Cancer Biology 2021Quote: ... hTERT-HME1 (ATCC CRL-4010) were cultured in Mammary Epithelial Cell Basal Medium (MEBM) supplemented accordingly to manufacturers (LONZA). HCC1954 cells (ATCC CRL-2328 ...
-
bioRxiv - Developmental Biology 2021Quote: ... 2 × 105 organoid single cells were re-suspended with Lonza P3 nucleofection buffer and 1 µl of pmaxGFP (Lonza) and transferred to 20 µl nucleofection cuvette (V4XP-3024 ...
-
bioRxiv - Immunology 2021Quote: ... Murine T cells were electroporated using the DN110 program of 4D nucleofector (4D-Nucleofector Core Unit: Lonza, AAF-1002B), human CAR T cells using the program E0115 ...
-
bioRxiv - Genetics 2020Quote: ... THP1-CAS9 cells were nucleofected with sgRNA using a protocol recommended for THP1 (Lonza, Amaxa 4D Nucleofector, Protocol #292). Cutting efficiency for each guide was measured using GeneArt Genomic Cleavage Detection Kit (ThermoFisher ...
-
bioRxiv - Cancer Biology 2020Quote: ... E4ORF1-transduced primary human umbilical vein endothelial cells (HUVECs) were cultured using the EGM-2 Bullet Kit (Lonza, Germany). All cell lines were kept at 37°C and 5% CO2 in a fully humidified incubator and negatively tested for mycoplasma by PCR.
-
bioRxiv - Biochemistry 2021Quote: ... Cells were thawed and cultured according to the manufacturer’s instructions using the recommended hepatocyte culture media provided by Lonza. Hepatocytes were seeded at a density of 2×106 cells per well in a 6-well plate coated with collagen type I (Corning ...
-
bioRxiv - Systems Biology 2021Quote: ... Cells were transfected with 250 nM of siRNA and 1 μl of control pMax GFP (Nucleofector X kit, Lonza), using the CU-133 program ...
-
bioRxiv - Molecular Biology 2021Quote: The expression plasmids for the HA-V5-FLAG-dCas9 bait and the dCas9-VPR were delivered to J-Lat 11.1 cells by nucleofection using Amaxa Nucleofector (Lonza) as previously described (3 ...
-
bioRxiv - Cancer Biology 2021Quote: MG-63 cells (1000 per well, 24-well plate) were seeded into 0.4% low melting point agarose (Lonza, 50101) on top of a 1% agarose layer ...
-
bioRxiv - Microbiology 2020Quote: ... Stock titer was determined by plaque assay (described below) on HeLa cells overlaid with MEM (Lonza, Walkersville, MD, USA) + 0.5% agarose (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2020Quote: ... EBs were collected and transferred into 6-well cell culture plate (12-16 EBs/well) in X-VIVO15 (Lonza) supplemented with IL-3 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: Vero cells were plated at 2.5 x 105 cell/well in 24-well plates in Essential-modified Eagle Medium (EMEM, Lonza) supplemented with 10% fetal calf serum (FCS ...
-
bioRxiv - Cell Biology 2021Quote: ... Infections for plaque assay in Vero E6 cells were performed in Eagle’s minimal essential medium with 25 mM HEPES (EMEM; Lonza) supplemented with 2% FCS ...
-
bioRxiv - Cell Biology 2021Quote: Vero cells were transfected with 2 µg plasmid DNA in the Amaxa Nucleofector solution R (Lonza Bioscience, Rockville, MD), following the manufacturer’s instructions with program V-01 ...
-
bioRxiv - Cell Biology 2021Quote: ... siRNA transfected primary XP186LV (XP-C patient cells) were serum-deprived for at least 24 hours in Ham’s F10 (Lonza) containing 0.5% FCS and antibiotics to arrest cells in G0 ...
-
bioRxiv - Neuroscience 2022Quote: ... 1 x 106 NSCs were mixed with 2.5 µg of corresponding DNA using Amaxa P4 Primary Cell 4D-Nucleofector X Kit S (Lonza) with CA137 programme in a 4D-Nucleofector X Unit (Lonza) ...
-
bioRxiv - Bioengineering 2022Quote: ... HUVECs were obtained from Lonza™ and cultured in Endothelial Cell Growth Medium-2 (EGM-2, PromoCell® C-22111 or Lonza™ CC-3162). Human primary pericytes were purchased from PromoCell® (PromoCell® ...
-
bioRxiv - Neuroscience 2022Quote: ... The BBB working medium was prepared using EGM-2 Endothelial Cell Growth Medium-2 BulletKit (Lonza, cat. #CC-3162), which includes EBM-2 Endothelial basal medium and endothelial growth supplements hEGF ...
-
bioRxiv - Immunology 2022Quote: Mammalian cells used in this study include BSC-40 (ATCC CRL-2761) (in Dulbecco minimal essential medium, DMEM, Lonza), healthy human periphery CD14+ monocytes (Lonza ...
-
bioRxiv - Molecular Biology 2022Quote: ... All cells were free of Mycoplasma contamination as confirmed by routine testing using the MycoAlert Mycoplasma Detection Kit (Lonza).
-
bioRxiv - Microbiology 2022Quote: ... and resuspended in 100 μL nucleofector solution at a concentration of 1.5 x 106 cells/100 μl following the instructions of the Nucleofector Kit V (Lonza). In vitro transcribed RNA (5 μg ...
-
bioRxiv - Immunology 2022Quote: ... and human monocytic leukemia cell line THP-1 (ATCC TIB-202) were cultured in RPMI-1640 (Lonza, 12-115F) containing 10% fetal bovine serum (FBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... Mycoplasma contamination of the cells were checked monthly using the MycoAlert Plus Mycoplasma Detection Kit (cat# LT07-703) (Lonza) following the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2022Quote: ... and 1 million cells were transfected with 2μg of donor vector and 2μg of each AAVS1 ZFN expression plasmids using the P3 Primary Cell 4D-Nucleofector X Kit (Lonza). Cells were seeded in E8 medium supplemented with 10µM ROCK Inhibitor Y-27632 (Selleckchem) ...
-
bioRxiv - Cancer Biology 2022Quote: ... 50 000 to 100 000 cells per reaction was then resuspended in 20uL of P3 solution (Lonza, V4XP-3032) and mixed by pipetting with the full gRNA-Cas9 complex mix previously assembled then added in the electroporation chamber well (Lonza ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were maintained in culture for the indicated time in the following medium: X-VIVO 10 (Lonza, BE04-380Q) supplemented with 20% BIT 9500 Serum Substitute (StemCell technologies ...
-
bioRxiv - Developmental Biology 2020Quote: ... expressing Cas9 and single sgRNAs targeting upstream and downstream regions of the lncRNA promoter/locus were co-transfected into 1.5 × 106 H1 hESCs using the Human Stem Cell Nucleofector Kit 2 (Lonza) and the Amaxa Nucleofector II (Lonza) ...
-
bioRxiv - Microbiology 2020Quote: ... Cell pellets were carefully enrobed in an equal volume of molten 5% low-melting temperature agarose (Lonza, Rockland, ME) and allowed to cool ...
-
bioRxiv - Microbiology 2020Quote: ... cells were nucleofected using Nucleofector SE solution and reagent into Nucleocuvette following manufacturer’s instructions using 4D-Nucleofector System (Lonza). The cell number and nucleic acid amounts are indicated in each figure legends ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cells were washed with RPMI before being re-suspended in Lonza electroporation solution from Kit C (Lonza, Basel, Switzerland) and electroporated using program W-001 using the Amaxa nucleofector (Lonza ...
-
bioRxiv - Immunology 2020Quote: ... DCs were seeded in a 24-well plate at a density of 1× 106 cells/well on 1.5mm coverslips (Warner Instrument) and cultured in complete phenol-red free RPMI 1640 media (Lonza) for 30 minutes at room temperature ...
-
bioRxiv - Biochemistry 2021Quote: ... or ds-UM DNA oligos (IDT) (Table S4) were nucleofected into mouse ES cells using an Amaxa nucleofector (Lonza) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were tested for Mycoplasma contamination every 4 - 6 weeks and before each experiment (Mycoalert Mycoplasma Detection kit, Lonza).
-
bioRxiv - Bioengineering 2021Quote: ... CD3+ T cells were debeaded with an EasySep magnet (STEMCELL) prior to resuspension and electroporation at 0.5-1×106 cell mL−1 in P3 buffer (Lonza). Following electroporations with RNPs and HDRTs ...
-
bioRxiv - Bioengineering 2021Quote: ... Approximately 1.5e6 K562 cells were electroporated in a final volume of 20 µL in a 16-well nucleocuvette strip (Lonza). For the T-cell experiments ...
-
bioRxiv - Immunology 2022Quote: ... The solution was centrifuged at 400G for 5 minutes at 4°C and the cell pellet was resuspended in 1mL of Hank’s Balanced Salt Solution (HBSS; Lonza) containing 1% BSA (Sigma Aldrich ...
-
bioRxiv - Cancer Biology 2022Quote: ... The cell lines were authenticated and tested for mycoplasma contamination regularly using MycoAlert mycoplasma detection kit (Lonza, NJ, USA). The cumulative culture length of cells between thawing and use in this study was less than 20 passages.
-
bioRxiv - Immunology 2022Quote: NIH3T3 WT fibroblast cells (3T3) and human CD40L-expressing 3T3 (Urashima et al., 1996) were cultured in IMDM (Lonza) containing 10% FCS (Bodinco) ...
-
bioRxiv - Microbiology 2022Quote: ... NHBE cells were infected with viruses at an MOI of 0.01 and cultured in B-ALI growth medium (Lonza) at 33°C ...
-
bioRxiv - Developmental Biology 2019Quote: ... were nucleofected into 1×106 pluripotent RUES2 cells using the B-016 setting on an Amaxa Nucleofector II (Lonza). Nucleofected cells were then plated as per maintenance conditions ...
-
bioRxiv - Molecular Biology 2019Quote: ... were obtained from Lonza (CC-2581) and maintained for no more than 8 passages in Smooth Muscle Cell Growth Media (SM-GM2, Lonza) supplemented with 5% FBS and 1X Penicillin/Streptomycin (Gibco) ...
-
bioRxiv - Bioengineering 2019Quote: ... Transfection of transposon and transposase vectors was performed on day 2 with 0,5 µg transposon MC and 2 µg SB100X transposase RNA per 1×106 T cells on a 4D-Nucleofector according to the manufacturer’s instructions (Lonza). Transfected CD8+ and CD4+ T cells were expanded using the G-Rex culture system (Wilson-Wolf) ...
-
bioRxiv - Genetics 2019Quote: ... Human multipotent adipose-derived stem cells (hMADS) were cultured in growth medium which consisted of: DMEM (Lonza, BE12-707F), 10% FBS ...