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Citations for Lonza :
3501 - 3550 of 4130 citations for Cytomegalovirus Cell Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 2×105 NSCs for essential gene expression were nucleofected with 750 ng each of the above-mentioned plasmids using SG Cell Line 4DNucleofector X Kit (Lonza), DN-100 program ...
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bioRxiv - Cell Biology 2022Quote: A GFP sgRNA vector was delivered into Cas9 and GFP expressing NSCs using nucleofection with the SG Cell Line 4DNucleofector X Kit (Lonza) as per manufacturer’s instructions51 ...
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bioRxiv - Biochemistry 2022Quote: ... 2×105 143B or HEK293T cells were resuspended in the resulting solution containing ribonucleoprotein complexes (RNPs) and electroporated using a 4D-Nucleofector (Amaxa, Lonza) programs FP-133 (143B ...
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bioRxiv - Pathology 2022Quote: The ability of CTEPH ECs to form tube-like structures on basement membrane matrix was compared to control human pulmonary artery endothelial cells (PAECs) obtained commercially (Lonza). The Cultrex® Reduced Growth Factor Basement Membrane Matrix (Trevigen cat.no ...
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bioRxiv - Immunology 2022Quote: ... IFNAR KO A549 cells were generated by CRISPR-Cas9 ribonucleoprotein (RNP) complex (IDT) transfection using the Nucleofector system (Lonza Bioscience). Specifically ...
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bioRxiv - Immunology 2022Quote: ... The digested vessels were then centrifuged and suspended in EGM™-2 Endothelial Cell Growth Medium-2 BulletKit™ (LONZA) supplemented with GlutaMax (ThermoFisher ...
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bioRxiv - Bioengineering 2022Quote: ... and 1 μg of a plasmid encoding a hyperactive piggyBac transposase (hyPBase) 39 were co-electroporated into Im-HUVEC using the P5 Primary Cell Kit (Lonza) and a 4D-nucleofector (Lonza) ...
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bioRxiv - Bioengineering 2022Quote: ... All cells were routinely verified as free of mycoplasma contamination using the MycoAlert mycoplasma detection kit (Lonza Group, Basel, Switzerland). MDA-MB-231 cells were first transduced with lentivirus (MOI=5 ...
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bioRxiv - Genetics 2022Quote: ... Cells were dissociated using accutase and 1 x 106 cells in P3 buffer were mixed with the RNP/template complex and nucleofected in large cuvettes (V4XP-3024 Lonza) using 4D-Nucleofector on program CA137 ...
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bioRxiv - Bioengineering 2022Quote: Two donors of human mesenchymal stem cells (hMSCs) at passage 5-6 were seeded on mineralized collagen scaffolds for osteogenesis experiments (seeded separately, BM-17, Lonza, Maryland ...
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bioRxiv - Cell Biology 2022Quote: ... HEK-293T and U-2OS (ATCC, Manassas, Virginia) cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Lonza, Basel, Switzerland) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2022Quote: ... and NP-C1 patient fibroblasts (GM18393 and GM17919A) were purchased from the Coriell Cell Repository (Table S1) and cultured in DMEM (Lonza) supplemented with 20% FBS (Bovogen) ...
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bioRxiv - Cell Biology 2022Quote: ... human astrocytoma CCF-STTG1 (ATTC CRL-1718) and human glioblastoma cell line U87-MG (ATCC HTB-14) purchased from Lonza (Lonza Walkersville ...
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bioRxiv - Bioengineering 2022Quote: ... Human hepatic stellate cell LX-2 was cultured in HEPES-buffered Dulbecco’s modified Eagle medium (DMEM) (Lonza BioWhittaker, Verviers, Belgium) supplemented with 4 mmol/L L-glutamine (Lonza) ...
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bioRxiv - Genomics 2022Quote: ... hiPSC-derived epicardial cells were dissociated with Accutase and plated at a density of 104 cells/cm2 in 6-well plates and cultured in fibroblast growth medium (Lonza) supplemented with 20 ng/ml FGF2 and 10 μM SB431542 ...
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bioRxiv - Immunology 2022Quote: ... activated-DC were washed twice in PBS 1X and put in culture with allogeneic naive CD4+ T cells (104 DC and 5×104 T) in X-VIVO 15 media (LONZA) for the indicated time ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lines in culture were screened biweekly for mycoplasma contamination using the MycoAlert PLUS Mycoplasma Detection Kit (Lonza, #LT07-703).
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bioRxiv - Cell Biology 2022Quote: ... Cells were seeded on protein-coated wells (9.4 x 104 cells/cm2) in either AimV/SF medium (FN-null MEFs) or Small Airway Epithelial Basal Medium (CC-3119; Lonza) in the absence or presence of EDTA (10 mM) ...
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bioRxiv - Cell Biology 2022Quote: ... we used Cas9-RNP and the Lonza 4D-Nucleofector System together with the P4 Primary Cell 4D-Nucleofector X Kit L (Lonza) to transfect and create Ndrg2-KO DCs ...
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bioRxiv - Cell Biology 2022Quote: ... For nucleofection we used the 4D-Nucleofector System and P3 Primary Cell 4D Nucleofector X Kit L (Lonza, Basel, Switzerland). We optimized the RNP electroporation for DCs by using multiple-guide mixtures and different number of cells in a 100uL total volume ...
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bioRxiv - Immunology 2020Quote: ... 5 µg of NOSIP shRNA plasmids or control TRC2 plasmid was mixed with 100 µl Cell Line Nuceleofector Solution V (Lonza) and added to one million cells ...
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bioRxiv - Cell Biology 2021Quote: ... Both endothelial cell lines (HUVEC, hPAEC) were grown in EBM-2 media with EGM-2 bullet kit (purchased from Lonza). No additional serum was used for cell culture ...
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bioRxiv - Neuroscience 2020Quote: ... control and C9orf72 iPSNs were nucleofected using the Lonza nucleofection system (program DC104) and a Lonza P3 Primary Cell 4D Nucleofector Kit (Lonza) as described above ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HUVECs were cultured in Endothelial Cell Growth Medium-2 BulletKit (EGM-2, cat. no. CC-3162, Lonza Inc., ME, USA). THP-1 cells were cultured in RPMI-1640 medium ...
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bioRxiv - Microbiology 2020Quote: ... for Vero-118 cells and serum reduced (2%) DMEM supplemented with 100 IU/ml penicillin-100 μg/ml streptomycin mixture (Lonza), 2 mM L-glutamine (Lonza) ...
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bioRxiv - Microbiology 2021Quote: Constructs co-expressing full-length hACE2 or cACE2 with mCherry2 protein (CMV promoter-ACE2-IRES-mCherry2) were generated and transfected into MDCK-SIAT1 cells via electroporation with Lonza Nucleofector system (Lonza) using the manufacturer’s protocol with program A024 ...
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bioRxiv - Neuroscience 2021Quote: ... dissociated with Accutase and then transfected with one of the Tau or Tubulin pUCM vectors along with AAVS1 TALEN pairs using a Human Stem Cell Nucleofector Kit 1 (Lonza) with the Nucleofector 2b Device (Lonza) ...
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bioRxiv - Immunology 2020Quote: ... Cells were then counted and frozen at 10×106 cells/ml of freezing media (10% DMSO in fetal bovine serum) (Lonza). and stored in liquid nitrogen until further use.
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bioRxiv - Neuroscience 2021Quote: ... Cells were transfected by combining 5×105 viable cells with 400 ng plasmid DNA and nucleofection solution in a 25-μL electroporation cuvette (Lonza). Cells were electroporated according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2021Quote: ... SK-N-BE(2) or H460 cells were seeded at densities mentioned above in ultra-low adherent round-bottom 96-well plates (Lonza) for 72 hours ...
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bioRxiv - Immunology 2022Quote: ... 1*106 purified naïve T-cells were resuspended in 15 μL buffer P3 + 5 μL Supplement 1 (P3 Primary Cell 4D-NucleofectorTM X Kit S; Lonza) and mixed with 2.7 μL RNP in the 16-well strips provided in the kit ...
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bioRxiv - Genetics 2022Quote: ... Libraries were electroporated into K562 cells in 100ul volumes using the Amaxa system (Program X-001, Neucleofactor kit V, Lonza). We performed 6 independent replicates with each replicate consisting of ∼2×108 cells ...
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bioRxiv - Immunology 2022Quote: ... was used to enrich in CD4+ or CD8+ T cells from PBMCs that were cultured in X-vivo 15 media (Lonza) supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... Knockdown of Vps4a/b was performed using Amaxa Nucleofector II and the Mouse T-Cell Nucleofector Kit (Lonza, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... LSECs were counted and 105 cells were seeded in a 48-well plate and cultured in collagenated plates with EGM-2 medium (Lonza) for 3 days ...
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bioRxiv - Biochemistry 2022Quote: Generation of overexpressing POLIC variant cell lines was previously described.10 10 μg pLEW79IA-MHTAP was NotI linearized and transfected into 29-13 procyclic cells by electroporation.8 7.5 μg pLew100-PTPPuro plasmids encoding full length POLIB and POLID variants were NotI linearized and transfected into 29-13 cells via Amaxa Nucleofection (Parasite Kit, Lonza). Following selection with 1 μg/mL puromycin for pLew100 based constructs and 2.5 μg/mL phleomycin for cells transfected with pLEW79IA-MHTAP ...
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bioRxiv - Immunology 2019Quote: ... The superior right lung lobe was mashed through a cell strainer and treated with Ammonium-Chloride-Potassium (ACK) lysis buffer (10-5483, Lonza). Cells were pelleted ...
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bioRxiv - Biophysics 2019Quote: ... Directed differentiation of iPSCs into cardiomyocytes was triggered at 80-90% cell density by adding the RPMI 1640 medium (Lonza) contained B27 supplement minus insulin (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2019Quote: ... mCherry-transfected) were cultured at 37°C in 5% CO2 in EGM®-2 Endothelial Cell Growth Medium-2 (Lonza). Patient-derived glioblastoma multiform (GBM ...
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bioRxiv - Cell Biology 2019Quote: Human umbilical vein endothelial cells (HUVECs) were cultured in EBM-2 media supplemented with the SingleQuotsTM kit (Lonza, Slough, UK). 3T3 fibroblasts were cultured in high glucose DMEM (invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... and a guide RNA targeting Tp53 (Wang et al., 2015) were transfected into cells using a Nucleofector 2b Device (Lonza) with the setting T023 ...
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bioRxiv - Cancer Biology 2019Quote: ... The cell lines were tested for the absence of mycoplasma using the MycoAlert™ Mycoplasma Detection Kit (Lonza, Allendale, NJ). Cell lines were also authenticated by Genetica DNA Laboratories (Burlington ...
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bioRxiv - Bioengineering 2019Quote: Human umbilical vein endothelial cells (HUVECs) were purchased form Lonza (C2519A) and cultured in EGM-2 media (Lonza; CC-3162). HUVECs and Stage 6 clusters containing SC-β cells were dispersed with TripLE Express and plated into a 6-well plate on an Orbi-Shaker at 100 rpm ...
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bioRxiv - Genetics 2020Quote: ... hESCs were nucleofected with 2 μg of CRISPR and 3 μl ssODN (100 μm) using the P3 Primary Cell Kit L (Lonza). hESCs were then plated onto puromycin-resistant (DR4 ...
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bioRxiv - Bioengineering 2020Quote: DNA delivery was carried out on 20 μL volume format using Amaxa 4D-Nucleofector System and Cell line nucleofector solution SF (Lonza). Total amount of DNA electroporated was 3 μg (1.5 μg each plasmid in the case of using two plasmids at the same time) ...
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bioRxiv - Cell Biology 2019Quote: ... HUVE cells were cultured in endothelial growth medium supplemented with 5% fetal bovine serum (FBS) and growth factors (EGM-2; Lonza). HEK293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Microbiology 2019Quote: ... In vitro transcribed RNA derived from wt or mutant pEAN900 was electroporated into BHK-21 cells using Amaxa Nucleofector technology (Lonza), as described previously (Beerens and Snijder ...
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bioRxiv - Developmental Biology 2020Quote: ... The guide RNA vector was then electroporated into the parent line containing the CRISPRi system using the Human Stem Cell Nucleofector Kit 1 solution with the Amaxa nucleofector 2b device (Lonza). Nucleofected cells were then seeded into a 6-well plate in mTeSR™-1 supplemented with Y-27632 (10 μM ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stimulated by CD3/CD28 Abs for 2 days and nuclofected with 100 μM RORC or non-targeting (NT1) siRNA (ON-TARGETplus SMART pool, Dharmacon) using the Amaxa Human T cell Nucleofector Kit (Amaxa, Lonza), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... pelleted at 350 xg for 10 minutes and resuspended in EBM-2 Basal Medium (CC-3156, Lonzs, Basel, Switzerland) supplemented with Microvascular Endothelial Cell Growth Medium-2 SingleQuots Supplements (CC-4147, Lonza) and 15% fetal bovine serum ...