Labshake search
Citations for Worthington Biochemical :
151 - 200 of 220 citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... ears of wild-type C57BL/6J mice were collected and mechanically disrupted before enzymatic dissociation in 10 mg/ml Collagenase IV (Worthington, LS004183), 0.2% FBS and 0.2 mg/ml DNase I (Worthington ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004183, 5 gm, $900.00 Ask -
bioRxiv - Neuroscience 2019Quote: ... 0.16mM NaOH pH=7.4) that contained 10 U/mL papain and 5mg of L-cysteine in total (Worthington, Lakewood, NJ, USA) for 10 minutes at 37°C ...
-
bioRxiv - Immunology 2021Quote: CHO cells stably expressing wild-type SARS-CoV-2 S were resuspended in wash buffer (PBS 1 % BSA, 2 mM EDTA) and treated with 10 µg/mL TPCK-trypsin (Worthington Biochem) for 30 min at 37°C ...
-
bioRxiv - Immunology 2020Quote: ... and both fragments were separately digested in a petri-dish at 37°C 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (12 hours) ...
-
bioRxiv - Cancer Biology 2022Quote: ... which included a first step of PDX tumor mechanical dissociation with sterile scalpels and then enzymatic dissociation by trypsin (10 mg/ml) and type II collagenase (275 U/mg, Worthington Biochemical). The tube was then placed in a warm 37°C water bath for 60 min ...
-
bioRxiv - Neuroscience 2023Quote: ... Hippocampi were isolated from P0 CD-1 mouse brain tissues and digested with 10 U/mL papain (Worthington Biochemical Corp., LK003176). Mouse hippocampal neurons were plated on following poly lysine-coated dishes for each experiment - glass bottom dishes (500,000 cells ...
-
bioRxiv - Cell Biology 2023Quote: ... the tissues were further transferred into 20 mL of pre-warmed digestion buffer (RPMI medium containing 10% FBS, 1% P/S, 15 mM HEPES, 25 U/mL of collagenase IV (Worthington LS004186), 100 U/ml of DNase I (Sigma D4513) ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Bioengineering 2023Quote: ... hACs were isolated using 10 ml of a 0.15% (w/v) type II collagenase (300 U mg−1; Worthington Biochemical Corporation) solution per g of tissue (37 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... The tissue was then washed with PBS and digested for 10 min at 37°C in 3 mg/ml collagenase II (Worthington, LS004176) in HBSS supplemented with 5% FBS and 10 mM HEPES ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Genomics 2023Quote: ... Each sample was centrifuged at 1200 rpm x5 mins at 4°C and resuspended in 10 mL warmed digestive media (HBSS + 1% HEPES) with 1,000 U/mL collagenase type IV (Worthington, Cat: LS004188), and rotated for 30 min at 37 °C ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Cortices were isolated from P0 CD-1 mouse brain tissues and digested with 10 U/mL papain (Worthington Biochemical Corp., LK003176). Mouse cortical neurons were plated on following poly lysine-coated glass bottom dishes (500,000 cells) ...
-
bioRxiv - Bioengineering 2024Quote: ... hippocampi were dissected from brains of newborn pups (P0) and digested for 20 min at 37 °C with 10 units of papain (Worthington, USA), followed by gentle mechanical trituration ...
-
bioRxiv - Immunology 2024Quote: ... mice were perfused with 1X PBS and the CNS was isolated into 10 mL of enzyme digestion solution consisting of 75 µL Papain suspension (Worthington, #LS003126) diluted in enzyme stock solution (ESS ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Cancer Biology 2019Quote: Xenografted tumors were dissected and mechanically dissociated using a papain dissociation kit (Worthington Biochemical Corporation ...
-
bioRxiv - Neuroscience 2023Quote: ... Hippocampal regions were dissected and dissociated with the Worthington Papain Dissociation Kit (Worthington Biochemical Corporation ...
-
bioRxiv - Bioengineering 2022Quote: ... detached from the filters and enzymatically digested with papain digestion kit (Worthington, LK003163) with the addition of DNase for 30 mins at 37°C ...
Product cited:
Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).Cat# LK003163, 3 bx, $715.00 Ask -
bioRxiv - Neuroscience 2022Quote: ... Half cerebella were processed using the Cell Dissociation Kit (Worthington Biochemical Corporation, #LK003150), in which samples were treated with papain at 37°C for 15min and then separated by centrifugation of an ovomucoid inhibitor density gradient ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2022Quote: ... Tumors samples were dissociated using the Cell Dissociation Kit (Worthington Biochemical Corporation, #LK003150), which included dissociation with papain at 37°C for 15min ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Developmental Biology 2023Quote: ... Retinae were dissociated using the Papain Digestion Kit (Worthington Biochemical Corporation, Lakewood, NJ) to obtain single cells as per vendor instructions.
-
bioRxiv - Neuroscience 2024Quote: ... Tissue chunks were then transferred to the PDS Kit-Inhibitor solution (#LK003182, Worthington) and filtered through strainers ...
Product cited:
Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and...Cat# LK003182, 1 vi, $86.00 Ask -
bioRxiv - Bioengineering 2021Quote: The elastin degraded model was produced by incubating native porcine carotid arteries in 10 U/ml purified elastase (ESFF, Worthington Biochemical Corporation) with 0.35 mg/ml trypsin inhibitor (10109886001 ...
-
bioRxiv - Immunology 2021Quote: ... lungs were minced with a scalpel and resuspended in 10ml GKN + 10% fetal calf serum (FCS) with collagenase IV (Worthington Biochemical Corp.) and DNase (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2022Quote: Hearts of C57BL/6N mice were harvested at an age of 8-10 weeks and subjected to digestion by perfusion via the coronary arteries using Collagenase II (Worthington, Lakewood, NJ) as described previously62 ...
-
Acinar-to-ductal metaplasia in the pancreas requires a glycolytic switch and functional mitochondriabioRxiv - Cancer Biology 2022Quote: ... specimens were cut into pieces and digested twice with 10 ml/g tissue of 50 U/ml collagenase (CLSPA Worthington Biochemical Corporation) in SOL1 for 10 min ...
-
bioRxiv - Neuroscience 2023Quote: ... Retinas were detached from the retinal pigmented epithelium and incubated for 15 min at 37°C in a solution containing papain (10 U/ml, Worthington; Lakewood, NJ) and L-Cysteine hydrochloride (∼1 mg/mL ...
-
bioRxiv - Genetics 2020Quote: ... cells were dissociated from transwells with a papain dissociation kit (Worthington Biochemical, Cat# LK003150) and filtered using a Flowmi cell strainer (Bel-Art SP Scienceware ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... by sectioning the optic nerves and were dissociated using the papain kit from Worthington Biochemical Corporation ...
-
bioRxiv - Neuroscience 2023Quote: ... Neurons were isolated from cortical tissue using the papain dissociation kit (Worthington Biochemical Inc.) as previously described [24] [25] and cells were plated at a density of 175,000 cells/cm2 ...
-
bioRxiv - Immunology 2021Quote: ... Collected lobes were incubated for one hour at 37°C in 10 mL of cRPMI with 1500 units of 260 units/mg type 1 collagenase (Worthington Biochemical, Cat. #LS004194) per lobe and pressed through a 35 µm filter ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Cell Biology 2022Quote: Cells were isolated from semitendinosus muscles of 10 Belgian Blue cattle (Supplementary Table 1) by collagenase digestion (CLSAFA, Worthington; 1 h; 37 °C), filtration with 100 μm cell strainers ...
-
bioRxiv - Microbiology 2022Quote: ... with 10% fetal calf serum and antibiotics/antimycotics supplemented with 1g/ml tosyl phenylalanyl chloromethyl ketone (TPCK)-trypsin (Worthington Biochemical Corp., Lakewood, NJ).
-
bioRxiv - Cancer Biology 2023Quote: ... spun down by centrifugation at 500 x g for 5 minutes and resuspended in 10 ml of digestion medium (MEM containing 112 U/mL type I collagenase; Worthington, cat. #CLS-1), 1.2 U/mL dispase (Gibco ...
-
bioRxiv - Immunology 2024Quote: ... and then incubated in 5 mL digestion buffer (basal media, 10 µM Y-27632, 600 U/mL Collagenase IV (Worthington cat. No. LS004189), 0.1 mg/mL DNAse I (Sigma-Aldrich ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004189, 5 gm, $900.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... and 250U ml−1 DNase in EBSS using the Papain Dissociation Kit (Worthington, Cat #:LK003150). The tissue was mechanically triturated and suspended cells were isolated and resuspended in EBSS with albumin-ovomucoid inhibitor solution ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Cancer Biology 2019Quote: ... single cells were prepared from the bulk tumor by a Papain dissociation kit (Worthington Biochemical) as per the manufacturer’s protocol and cultured using previously reported culturing methods76 ...
-
bioRxiv - Cancer Biology 2021Quote: ... E15-P30 Cerebella were dissociated with a Papain dissociation kit following the manufacturer’s instructions (Worthington). Following papain treatment ...
-
bioRxiv - Bioengineering 2022Quote: ... Then mechanically minced to 1mm2 pieces and enzymatically digested with papain digestion kit (Worthington, LK003163) with the addition of DNase for 1 hr at 37°C ...
Product cited:
Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).Cat# LK003163, 3 bx, $715.00 Ask -
Regulation of Diseases-Associated Microglia in the Optic Nerve by Lipoxin B4 and Ocular HypertensionbioRxiv - Molecular Biology 2024Quote: Retinas were dissociated into single-cell suspensions using a papain dissociation kit (Worthington, Columbus, OH). Briefly ...
-
bioRxiv - Neuroscience 2022Quote: ... Cortices were isolated from postnatal day 0 (P0) CD-1 or cGKII KO mouse brain tissues and digested with 10 U/mL papain (Worthington Biochemical Corp. Lakewood, NJ). Mouse cortical neurons were plated on following poly lysine-coated dishes for each experiment - 24 wells (200,000 cells ...
-
bioRxiv - Cell Biology 2019Quote: ... each consisting of calvaria from 6 pups were digested in 10 ml collagenase solution (1 mg/ml Collagenase type I and type II (1:3 ratio, Worthington Biochemical Corp., Lakewood, NJ), 1 mM CaCl2 ...
-
bioRxiv - Neuroscience 2023Quote: ... and digested at 37°C for 20 min with Collagenases-I and - IV (10 U/mL, 400 U/mL final, Worthington, Cat# LS004194, and LS004184) and DNase I (30 U/mL ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Neuroscience 2024Quote: ... ∼20 hCOs were chopped into small pieces using a #10 blade and treated with 30 U/ml Papain (Worthington Biochemical, CAS: 9001-73-4) for 60-90 minutes at 37 °C ...
-
bioRxiv - Neuroscience 2020Quote: ... modified to use early post-natal mice and to employ a commercial kit (Worthington Biochemical, LK003150). P1-P4 mice are sacrificed by guillotine method cut just caudal to the ears ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Developmental Biology 2024Quote: Cerebral organoids were dissociated into a single-cell suspension using Worthington Papain System kit (Worthington, LK003150) according to the manufacturer’s instruction ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Cancer Biology 2024Quote: ... Cells were spun down and supernatant was removed before the addition of 1 ml Dispase-mUE (Dispase II [7 mg ml-1 DMEM Ham’s F12], Neutral Protease, Worthington NPRO2; and 10 μg μL-1 DNase). Cells were aspirated in the solution gently 40 times using a 1 ml pipette tip ...
-
bioRxiv - Cancer Biology 2022Quote: ... Total tumor collected per mouse was dissociated using standard methods with a Papain dissociation kit (Worthington Biologicals). Following filtration through a 40 micron filter ...
-
bioRxiv - Genomics 2022Quote: Primary rat cortical neurons were prepared from E18-19 embryos using a papain dissociation kit (Worthington Biochemical) following the manufacturer’s instruction ...
-
bioRxiv - Neuroscience 2021Quote: Retinal cells were dissociated into single cells using a Papain dissociation kit (#LK003150, Worthington Biochemicals, Lakewood, NJ, USA), as described by us previously (Ahmed et al. ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2019Quote: ... retinal cells were dissociated into single cell suspensions using a Papain dissociation kit (Worthington Biochemicals, New Jersey, USA). 125 × 103 retinal cells/well were cultured in chamber slides (Beckton-Dickinson ...
-
bioRxiv - Neuroscience 2022Quote: ... and the Tissue pieces were dissociated for 30 min at room temperature using the Papain dissociation kit (Worthington) in calcium-free Hibernate A (BrainBits) ...