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Citations for Worthington Biochemical :
151 - 200 of 209 citations for Primary Human Coronary Artery Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The cortices were dissected out and dissociated into single-cell suspensions with a papain dissociation system (Worthington Biochemical Corporation) and plated onto poly-L-lysine (Sigma Aldrich)-coated plates (0.1% (wt/vol) ...
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bioRxiv - Neuroscience 2021Quote: ... pH adjusted to 7.6] and mechanically dissociated to single cells after adding ovomucoid (Worthington, 0.7 mg per 5 mice) and DNAse (Worthington ...
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bioRxiv - Molecular Biology 2020Quote: ... The cells were washed with the buffer and were incubated with 0.2 mg/mL DNase I (Worthington Biochemical, US) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... Niche cells from the bone marrow fraction were isolated by digestion with 1 mg/mL STEMxyme1 (Worthington, Ref#LS004106) and 1 mg/mL Dispase 1 (ThermoFisher Scientific ...
Product cited:
A specialized combination of Animal Free Clostridium histolyticum collagenase (Code: CLSAFB) and...Cat# LS004106, 50 mg, $110.00 Ask -
bioRxiv - Neuroscience 2022Quote: Individual brain organoids were dissociated into a single-cell suspension using the Worthington Papain Dissociation System kit (Worthington Biochemical). A detailed description of the dissociation protocol is available at Protocol Exchange ...
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bioRxiv - Neuroscience 2022Quote: Individual brain organoids were dissociated into a single-cell suspension using the Worthington Papain Dissociation System kit (Worthington Biochemical). A detailed description of the dissociation protocol is available at Protocol Exchange ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×106 HCT116 cells were used per each immunoprecipitation (IP) reaction and 15 units of micrococcal nuclease (MNase, Worthington) was used to digest the chromatin for the ChIP reaction ...
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bioRxiv - Bioengineering 2023Quote: ... or in vivo transplantation using an adaptation of a well-established neural cell isolation method (Worthington 9035-81-1).71 As with our undifferentiated hiPSC cultures ...
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bioRxiv - Bioengineering 2023Quote: Cells were isolated from semitendinosus muscles of Belgian Blue cattle by collagenase digestion (CLSAFA, Worthington; 1 h; 37 °C), filtration with 100 μm cell strainers ...
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bioRxiv - Immunology 2023Quote: ... Cells were then DNase treated with 4 ml disaggregation media (DMEM, 10% FBS, 100 μg/ml DNASE I (Worthington)) for 20 min at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: Cortex of E12.5 and E14.5 embryos was dissected and dissociated into single cells using the Papain Dissociation System (#LK0031500, Worthington Biochemical). Individual collected cells were processed for scRNAseq using ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Cancer Biology 2024Quote: ... Cells cultured on collagen and mineralized collagen were collected using collagenase type I (1mg/mL in PBS, Worthington Biochemical) and passed through a 40 µm cell strainer ...
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bioRxiv - Molecular Biology 2019Quote: ... of 0.1 focus-forming unit (FFU)/cell in M199 medium supplemented with 1 mg/mL porcine pancreatic trypsin (Worthington Biochemical). Cell culture medium was collected 24–36 h post infection ...
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bioRxiv - Cell Biology 2019Quote: ... A single cell suspension was made by digesting tissue for 30 minutes at 37°C in Collagenase type 2 solution (Worthington Biochemical ...
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bioRxiv - Neuroscience 2020Quote: ... NBS1- and NBS8-organoids were dissociated to a single cell suspension for 30 minutes using the Papain dissociation kit (Worthington) according to the manufactures’ instructions ...
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bioRxiv - Microbiology 2020Quote: ... All viruses were titrated using 1:10 serial dilution in triplicate in LLCMK2 cells in the presence of 2μg/mL TPCK trypsin (Worthington Biomedical) for 72 hours and TCID50/mL was determined by the Reed-Meunch method based on hemagglutination of 0.5% washed chicken red blood cells (Lampire ...
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bioRxiv - Cell Biology 2021Quote: ... The hypothalamus and pre-optic area were then dissected from vibratome sections and dissociated into single cells using the Papin Dissociation Kit (Worthington) as previously described24 ...
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bioRxiv - Bioengineering 2022Quote: ... cells showed spontaneous beating and were dissociated on day 15 by incubation with 280 U/ml Collagenase (LS004174, Worthington, USA) and 40 U/ml DNase (LS006331 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004174, 100 mg, $42.00 Ask -
bioRxiv - Cell Biology 2020Quote: ... were generated using the eight plasmid-based systems as previously described (Wit et al., 2004) and propagated on MDCK-II cells in presence of TPCK-trypsin (Worthington). For infection ...
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bioRxiv - Microbiology 2019Quote: ... was propagated in MA104 cells in serum-free DMEM in the presence of 1 µg/mL Worthington’s Trypsin (Worthington Biochemical). After harvest ...
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bioRxiv - Cancer Biology 2020Quote: A total of ten tumor specimens were dissociated into a single cell suspension using a Papain Dissociation System (The Worthington Papain Dissociation System ...
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bioRxiv - Neuroscience 2023Quote: ... Individual cells were isolated by enzymatic digestion with 0.5 mg/ml neutral protease and 0.5 mg/ml elastase (Worthington Biochemical Corporation) in endothelial cell (EC ...
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bioRxiv - Developmental Biology 2023Quote: ... washed with PBS and dissociated into single cells using collagenase type 4 enzyme at 200Units/ml (Worthington, Lakewood, NJ, US) for 10min at 37°C ...
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bioRxiv - Immunology 2023Quote: T cells that infiltrate pancreatic islets were isolated after intra-bile duct injection and digestion with collagenase IV (Worthington Biochemical) 28 ...
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bioRxiv - Cancer Biology 2023Quote: ... and dissociated into single-cell suspensions with an enzymatic digestion buffer consisting of Collagenase-II (for subcutaneous tumors) (Cat: LS004176, Worthington) or Collagenase-III (for breast tumors ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... The L2-5 ganglia were dissected out and placed in cell culture media before being transferred to media containing neutral protease 3.125 mg/mL (Worthington, LS02104) and collagenase type 1 5 mg/mL (Worthington ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02104, 50 mg, $352.00 Ask -
bioRxiv - Evolutionary Biology 2023Quote: ... Ganglia were cut into small pieces and cells were dissociated by incubating ganglia pieces in enzyme solution (10 mg/mL collagenase IV (Worthington), in 1XHBS (HEPES buffered saline ...
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bioRxiv - Developmental Biology 2023Quote: ... Cortical organoids were dissociated into single-cell suspensions via 30-min incubation at 37 °C in papain and DNAse in EBSS (Cat. # LK003150, Worthington), shaking vigorously every 5-10 min ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Cancer Biology 2024Quote: ... Cells were crosslinked with 1% formaldehyde and then 2.5 x 106 cells were treated with 60 U of MNase (Worthington, LS004797) and Exonuclease III (E ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004797, 15 ku, $94.00 Ask -
bioRxiv - Genetics 2024Quote: Testicular cell suspension was prepared by incubation of seminiferous tubules in 200 U/ml Collagenase type I (Worthington Biochemical LS004196), 5 μg/ml DNAse I (Roche 10104159001) ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Molecular Biology 2020Quote: Cortical cell suspension were obtained by dissociating cerebral cortices from E18 embryos using the Papain Dissociation System (Worthington, cat. no. LK003150). One million live cells were plated in 5 ml complete Neurobasal culture medium (Neurobasal™ Medium (Gibco ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... The SVZ was then dissociated into single cell suspension following the manufacturer’s protocol in the papain dissociation kit (Worthington Biochemicals # LK003150)41 ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... Cell mixture is centrifuged 6 minutes at 300 x g and after carefully aspirating the supernatant the cell pellet is then resuspended with EBSS plus ovomucoid inhibitor albumin mixture supplemented with DNase (each part of Worthington kit). Gentle trituration with a 10 ml pipette held several centimeters from the tube bottom is sufficient to resuspend ...
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bioRxiv - Neuroscience 2021Quote: Five independent samples of midbrain organoids (Table S1) were gently dissociated to single cells with papain enzymatic dissociation (Worthington Biochemical Corporation). After filtering cell clumps with a cell strainer (35um mesh) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated at 37°C in serum-free cell culture medium (IMDM) supplemented with 1 µg/ml L-1-tosylamide-2-phenylethyl chloromethyl ketone (TPCK) trypsin (Worthington-Biochemical). Cell supernatants were harvested at indicated time points post-infection ...
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bioRxiv - Neuroscience 2022Quote: ... Each thalamus was cut into small pieces and dissociated into a single cell suspension using the Papain Dissociation System (Worthington, LK003153) according to the manufacturer’s protocol ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003153, 3 bx, $775.00 Ask -
bioRxiv - Neuroscience 2019Quote: Primary mouse hippocampal and cortical neurons were dissociated into single-cell suspensions from E16.5 mouse brains with a papain dissociation system (Worthington, Cat# LK003153). Neurons were seeded onto poly-L-lysine–coated plates (0.1% (wt/vol) ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003153, 3 bx, $775.00 Ask -
bioRxiv - Cancer Biology 2020Quote: Cells were derived from a minced piece of tumour digested in DMEM supplemented with 200U/ml collagenase type IV (Worthington, USA) at 37 °C for 24 h ...
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bioRxiv - Neuroscience 2023Quote: ... Pools of cortical organoids from each donor were enzymatically dissociated into a single-cell suspension using the Worthington Papain Dissociation System kit (Worthington Biochemical). Cell suspensions derived from different donors were mixed at equal ratios ...
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bioRxiv - Molecular Biology 2024Quote: ... nuclei from 2 X 108 Hela or 293T cells were digested with 20 U of micrococcal nuclease (Worthington Biochemical Corporation, LS004797) for 45 minutes ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004797, 15 ku, $94.00 Ask -
bioRxiv - Neuroscience 2020Quote: The organoids cultured from both 24w and 96w plates were dissociated into single cell suspension using a commercially available papain digestion kit (Worthington-biochem, LK003150). Live cells and dead cells from the dissociated organoids were stained with Calcium Green AM (ThermoFisher C3011MP ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
Pax6 loss alters the morphological and electrophysiological development of mouse prethalamic neuronsbioRxiv - Developmental Biology 2021Quote: ... the prethalamus from the two halves of the neural tube of the same embryo were put together and chopped into smaller pieces for dissociated cell culture using the Papain Dissociation System (Worthington Biochemical Corp.) according to the manufacturer’s protocol (Figure 2C-E) ...
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bioRxiv - Neuroscience 2019Quote: ... neural retinas were digested at 37°C for 9 minutes in papain solution and single cells were obtained following manufacturer instructions (Papain Dissociation System, Worthington Biochemical, USA). Living cells were counted using a hemocytometer after trypan blue staining and resuspended in PBS at a density of 1×105 cells/μl ...
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bioRxiv - Bioengineering 2021Quote: ... Muscles were minced to create a homogenous cell slurry and then incubated for 2 h at 37C in 0.2% collagenase (Worthington Biochemicals, Lakewood, NJ) in DMEM ...
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bioRxiv - Cell Biology 2020Quote: Lung samples from mice of different ages (P0-P62) were dissociated into a single cell suspension using the Papain Dissociation System (Worthington Biochemical Corporation) with a modified protocol ...
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bioRxiv - Genomics 2022Quote: ... Nuclei were collected by centrifugation and DNase I-treated for 30 min at 37°C (Worthington, 30U per 5 x 106 cells). After DNase I digestion ...
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bioRxiv - Developmental Biology 2023Quote: 30 hindbrains of st.18 chick embryos were harvested and disassociated into single cells using collagenase type 4 (200 Units\ml, Worthington 47B9407, USA). Cells were then centrifuged at 600 g for 10 min ...
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bioRxiv - Developmental Biology 2023Quote: ... 60 hindbrains of st.18 chick embryos were harvested and disassociated into single cells using collagenase type 4 (200 Units\ml, Worthington 47B9407, USA). Cells were then centrifuged at 600 g for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... 4–5 randomly selected organoids were pooled to obtain single cell suspension from hDiO and then incubated in 30 U/mL papain enzyme solution (Worthington Biochemical, LS003126) and 0.4% DNase (12,500 U/mL ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Molecular Biology 2023Quote: Uterine pieces were washed with phenol red-free free HBSS(−) to remove epithelial cells and further incubated in phenol red-free free HBSS(−) with collagenase type III (#CLS3, Worthington, Lakewood, NJ) at 37.5°C for 30 min ...