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Citations for Worthington Biochemical :
101 - 150 of 152 citations for Hyaluronidase PH 20 SPAM1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Micro-dissected regions were dissociated by incubating with papain (20 units/ml) DNase (0.005%) for 30min at 37°C (Worthington). Samples were triturated in HEPES buffered EBSS to fully dissociate the tissue and centrifuged (3 min ...
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bioRxiv - Biochemistry 2019Quote: ... The sections were transferred to 3mL of Hibernate E solution containing 20 units of Papain (# LK003178 Worthington-biochemical corporation) in Earle’s Balanced Salt Solution (EBSS ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Cell Biology 2020Quote: ... and then they were incubated in 20 mL of DMEM media containing 0.2 % collagenase type II (Worthington Biochemical Corporation) and 2.5 U/mL dispase (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... tumors were mechanically dissociated and subjected to enzymatic digestion for 20 min with 200 U/ml collagenase IV (Worthington), 5 mM CaCl2 ...
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bioRxiv - Neuroscience 2023Quote: ... The collected tissue was incubated with continuous shaking at 37°C for 45 min in 1 ml of papain solution (20 U/ml) and 0.2 ml DNase (both from Worthington) prepared in isolation buffer ...
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bioRxiv - Neuroscience 2022Quote: ... The pieces were dissociated first using the ice-cold oxygenated dissection buffer added papain (20 units/ml, Worthington, LS003126), 0.067 mM 2-mercaptoethanol (Sigma ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Physiology 2023Quote: ... The cortical tissue from each brain was pooled and digested in papain solution (20 U/mL Cat# LS003126, Worthington) and then treated with DNase I (Cat# LS006563 ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Cell Biology 2020Quote: ... tumors were mechanically dissociated and subjected to enzymatic digestion for 20 min with 200 U ml−1 collagenase IV (Worthington), 5 mM CaCl2 ...
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bioRxiv - Cell Biology 2020Quote: ... as well as on day 1 and 2 after RFA were dissociated using 20 U/ml papain43 (Worthington Biochemical, USA), filtered through a 70 μm filter and resuspended in 3% BSA in PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... Approximately 40-60 wing discs were dissociated in 250 μL of Papain (20 U/mL) and DNase I (200 U/mL) in Earle’s Buffered Salt Solution (all from Worthington Biochemical), at 25°C for 40 minutes ...
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bioRxiv - Developmental Biology 2020Quote: Prostate tissues were minced with scissors and then incubated in papain (20 units/ml) with 0.1 mg/ml DNase I (Worthington LK003150) at 37°C with gentle agitation ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... cerebella from litters of P6 mice were pooled and digested at 37 °C for 20 minutes in 10U ml−1 papain (Worthington), and 250U ml−1 DNase in EBSS using the Papain Dissociation Kit (Worthington ...
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bioRxiv - Immunology 2020Quote: ... Pieces were further digested for 30 min and then twice for 20 min with a mixture of Collagenase IV (157 Wuensch units ml−1, Worthington), DNAse I (0.2 mg ml−1 dissolved in PBS ...
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bioRxiv - Bioengineering 2020Quote: ... Cell suspensions were harvested by mechanical dissection and Collagenase IV Cocktail digestion (formulated as 7.4 kU Collagenase Type 4, 53 kU Deoxyribonuclease I, 20 mg Soybean Trypsin Inhibitor in 10 mL cocktail; Worthington Biochemical). The suspensions were passed through 40 μm cell strainers to ensure singularity ...
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bioRxiv - Bioengineering 2020Quote: ... The dissected strips of HTM tissue were digested for 20 min with 1 mg/ml collagenase (Worthington, Lakewood, NJ, USA) and 15 μl/ml human albumin (Sigma-Aldrich ...
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bioRxiv - Physiology 2022Quote: Digestion of the left kidney was performed by a 20 minutes-incubation in HBSS containing type II collagenase at 0.5 mg/ml (Worthington, USA), followed by a 4 minutes-incubation in trypsin 0.04% at 37°C after removal of the capsule ...
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bioRxiv - Neuroscience 2023Quote: ... and Plp1-TAT-DTR mice (for P42_new IHCs) were dissected out and first digested in a choline chloride solution containing 20 U/mL papain (Cat#: LK003178, Worthington) and 100 U/mL DNase I (Cat# ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Neuroscience 2023Quote: Cerebral cortices from P4-6 CD1 mouse pups were dissected in ice-cold HBSS without Mg2+ and Ca2+ and triturated with 2 ml of digestion buffer containing 20 U/ml papain (Worthington) and 100 U/ml DNase I (Worthington ...
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bioRxiv - Genetics 2022Quote: ... cortices and hippocampi acquired from 12 male mice from 3 separate litters were combined and dissociated using activated 20 U/mL Papain/DNase (Worthington) diluted in Hibernate A (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... and individually incubated at 37°C for 15 to 20 min in a sterile solution of 45 U papain (Worthington), 0.01% DNase (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... and individually incubated at 37 °C for 15 to 20 min in a sterile solution of 45 U papain (Worthington), 0.01% DNase (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... the hippocampi were washed twice with cold HBSS and individually incubated at 37°C for 20 min in Papain dissociation solution (45 U of papain (Worthington), 0.01% deoxyribonuclease (DNase) ...
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bioRxiv - Immunology 2024Quote: ... Enzymatic digestion was stopped with 1 mL of 10X hi ovomucoid inhibitor solution and 20 µL 0.4% DNase (Worthington, #LS002007) diluted in 10 mL inhibitor stock solution (ISS) ...
Product cited:
Chromatographically purified. A lyophilized powder with glycine as a stabilizer.Cat# LS002007, 100 mg, $350.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... transfer either only the cerebellum or the cerebellum including pones to a 15 mL tube containing 20 U/mL papain (Worthington, #LK003178) solved in preparation solution and warmed up to 36 °C ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... The tissue was digested for 30 min using 2 mL of HEPES-buffered HBSS containing 20 U/mL of papain (Worthington Biochem.), 1 mM EDTA (PanReac AppliCham ...
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bioRxiv - Neuroscience 2019Quote: ... following genotyping or embryonic day 18 embryos (rat) and dissociated enzymatically for 20 min at 37 °C in HBSS supplemented with 0.25% (w/v) trypsin (Worthington Cat# LS003707), followed by mechanical dissociation via trituration with fire-polished glass Pasteur pipettes ...
Product cited:
Supplied as a chromatographically purified, diafiltered and lyophilized powder.Cat# LS003707, 1 gm, $325.00 Ask -
bioRxiv - Cell Biology 2021Quote: ... Cortices were digested at room temperature for 1 hour using papain solution composed of 20 units/ml papain (Worthington Biochem, LS003126), 1.5 mM CaCl2 (Sigma Aldrich ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... was dissected on ice and minced using two razor blades in a few drops of pre-warmed (37°C) Hibernate A medium containing 20 units/mL papain (Worthington LK003178). Minced tissue was incubated in 3.125 mL pre- warmed papain solution for 10 minutes at 37°C ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Bioengineering 2024Quote: ... hippocampi were dissected from brains of newborn pups (P0) and digested for 20 min at 37 °C with 10 units of papain (Worthington, USA), followed by gentle mechanical trituration ...
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bioRxiv - Cancer Biology 2024Quote: ... Biopsies were mechanically minced with sterile scissors into fragments of less than 1 mm3 and enzymatically digested with 20 mg/mL papain (Worthington #20132), 0.7mg/mL hyaluronidase (Sigma #V900833 ...
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bioRxiv - Molecular Biology 2024Quote: ... nuclei from 2 X 108 Hela or 293T cells were digested with 20 U of micrococcal nuclease (Worthington Biochemical Corporation, LS004797) for 45 minutes ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004797, 15 ku, $94.00 Ask -
bioRxiv - Neuroscience 2022Quote: ... Tissue from 2-3 hypothalamiwas pooled into 1-1.5 mL of HABG containing papain (20 u/mL, Worthington Biochemical, Lakewood, NJ, US). Tissue was broken into smaller pieces by pipetting 2-3 times with a P1000 pipet followed by a fire-polished glass pipette with a wide opening ...
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bioRxiv - Neuroscience 2021Quote: ... The tissue was digested for 30 min using 2 mL of HEPES-buffered HBSS containing 20 U/ml of papain (Worthington Biochem., USA), 1 mM EDTA (PanReac AppliChem ...
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bioRxiv - Bioengineering 2022Quote: ... and then inflated via the trachea with 2-3 mL of pre-warmed (37°C) enzyme solution (1 mg/mL collagenase/dispase [Roche], 3 U/mL elastase [Worthington], 20 U/mL DNase [Worthington] in DMEM). The heart and large airways were dissected away and the remaining lung tissues and enzyme solution were collected and transferred to a shaking 37°C water bath at 75 rpm for 20 min ...
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bioRxiv - Bioengineering 2021Quote: ... The tissue was digested for 30 min using 2 mL of HEPES-buffered HBSS containing 20 U/ml of papain (Worthington Biochem., USA), 1 mM EDTA (PanReac AppliChem ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were minced into small pieces and transferred to a dounce-homogenizer containing 5 ml ice-cold HBSS with 20 µg/ml DNase I (Worthington, DPRF, LS006343). Tissue chunks were homogenized with 15 loose and 15 more tight strokes and the homogenate was transferred to a 50 ml falcon tube through a pre-wet 70 µm strainer ...
Product cited:
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. ...Cat# LS006343, 50 ku, $640.00 Ask -
bioRxiv - Molecular Biology 2022Quote: Five 10um cryostat slices of patient polyps were digested for 5 min at 37°C with a titration of MNase: 20 units/mL and 200 unit/mL of MNase (Worthington Biochemical Corp.) in MNase cleavage buffer (4 mM MgCl2 ...
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bioRxiv - Neuroscience 2023Quote: ... the medial PFC of ECFP-expressing P1 mice was dissected and dissociated with papain (20-25 U/mL; Worthington Biochemical, Lakewood, NJ) at 37°C for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... The collected cochlear sensory epithelia were incubated for 20 min at 37℃ in an enzymatic solution containing papain (LK003150, Worthington Biochemical Corp) and dissociated by triturating with a pipette ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... the cortical tissue was incubated with constant shaking at 37°C for 40 minutes in 1 ml of papain (20 U/ml) dissolved in the isolating solution supplemented with 60 µl of DNase (both from Worthington, Lakewood, NJ, USA). During the enzymatic dissociation ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissue fragments were incubated in 1.35 mL PBS plus 150 uL 20 mg/mL Collagenase Type I (Worthington CLS-1 dissolved in PBS) and 15 uL 20 mg/ml DNase I (Worthington D dissolved in PBS ...
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Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium IndicatorsbioRxiv - Bioengineering 2023Quote: ... Tissue was minced until 1mm pieces remained, then lysed in equilibrated (37°C, 5% CO2) enzyme (20 U/mL Papain (Worthington Biochemical Corp; LK003176) in 5mL of EBSS (Sigma ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Bioengineering 2024Quote: ... Tissue was minced until 1mm pieces remained, then lysed in equilibrated (37°C, 5% CO2) enzyme (20 U/mL Papain (Worthington Biochemical Corp; LK003176) in 5mL of EBSS (Sigma ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Bioengineering 2024Quote: ... Tissue was minced until 1mm pieces remained, then lysed in equilibrated (37°C, 5% CO2) enzyme (20 U/mL Papain (Worthington Biochemical Corp; LK003176) in 5mL of EBSS (Sigma ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Microbiology 2022Quote: Enzymatic digestion of lung tissue was performed by intratracheal instillation via a 20-gauge catheter of 1 mL of 5 mg/mL collagenase I (Worthington Biochemical Corp, Lakewood, NJ) and 0.25 mg/mL DNase I (Sigma ...
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bioRxiv - Immunology 2021Quote: ... Lungs were excised and processed by mincing with scissors followed by enzymatic digestion using 180 units/ml collagenase IV and 20 µg/ml DNase I (Worthington biochemical LS004188 and LS002139) in 5 ml RPMI incubated at 37°C for 30-45 mins in a shaker incubator before filtering through 70 µm filters and lysing RBCs with ACK lysis buffer ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2022Quote: ... The SNc regions were dissected out from each slice and incubated for 20–30 min at 36–37℃ in oxygenated high-glucose HEPES-buffered saline containing 4–8 U/ml papain (Worthington Biochemical Corp., USA, LK0003176). After enzymatic digestion ...
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bioRxiv - Neuroscience 2023Quote: ... and digested at 37°C for 20 min with Collagenases-I and - IV (10 U/mL, 400 U/mL final, Worthington, Cat# LS004194, and LS004184) and DNase I (30 U/mL ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Pharmacology and Toxicology 2024Quote: Lungs were digested by intratracheal instillation via a 20-gauge catheter of 1 mL of 5 mg/mL collagenase I (Worthington Biochemical Corp, Lakewood, NJ) and 0.25 mg/mL DNase I (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... ∼20 hCOs were chopped into small pieces using a #10 blade and treated with 30 U/ml Papain (Worthington Biochemical, CAS: 9001-73-4) for 60-90 minutes at 37 °C ...