Labshake search
Citations for Worthington Biochemical :
1 - 50 of 523 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... The RT units of a concentrated stock of HIV Q23.BG505 virus were determined multiple times using a standard curve of purified HIV RT enzyme (Worthington Biochemical Corp. #LS05003). Aliquots of this titered stock of Q23.BG505 were used as the quantitative standard curve in all assays ...
Product cited:
Chromatographically purified dimeric form with M.W. of 66 kDa and 51 kDa. A solution in 10 mM...Cat# LS05003, 200 un, $95.00 Ask -
bioRxiv - Molecular Biology 2022Quote: ... Recombinant HIV (Worthington) and HiScript III (Vazyme ...
-
bioRxiv - Biochemistry 2020Quote: Reverse transcription reactions with Moloney Murine Leukemia Virus (MMLV) RT (BioBharati Life Sciences Pvt. Ltd) and HIV RT (Worthington Biochemical) were carried out according to the manufacturer’s directions using primer P5 (Table S1) ...
-
bioRxiv - Microbiology 2020Quote: ... Media was changed 18-24 hours post-transfection, with 2 mL of virus growth media (MEM, 1 mM HEPES, 1 μg/mL TPCK trypsin (Worthington Biochemical Corporation ...
-
bioRxiv - Biochemistry 2020Quote: ... 5 U SUPERase•In™ RNase Inhibitor and 5 U HIV RT (Worthington Biochemical Corporation, cat. HIVRT). For TGIRT-III ...
-
bioRxiv - Microbiology 2019Quote: ... tissue supernatants were serially diluted 10-fold in virus infection medium supplemented with 1 µg/mL L-1-tosylamide-2-phenylethyl chloromethyl ketone (TPCK)-trypsin (Worthington Biochemical Corporation, Lakewood, NJ). The plates were rinsed twice with PBS ...
-
bioRxiv - Bioengineering 2020Quote: ... Infection with the H1N1 virus was performed in the presence of 1 µg of TPCK (tolylsulfonyl phenylalanyl chloromethyl ketone)-trypsin/ml (Worthington Biomedical).27 Briefly ...
-
bioRxiv - Microbiology 2023Quote: ... virus was activated by adding 8μg/mL trypsin (TPCK treated, Worthington, Lakewood, NJ) in FCS-free media to samples in a 1:1 ratio ...
-
bioRxiv - Cancer Biology 2024Quote: ... samples were digested with 1:50 (m/m) lysyl endopeptidase C (Fujifilm) at 42 °C for 2 hours and 1:50 (m/m) trypsin (Worthington) at 37 °C overnight ...
-
bioRxiv - Physiology 2021Quote: ... The proteins were digested for 5 hours with Lys-C (Wako) at room temperature and overnight with trypsin (Worthington). Samples were acidified and diluted with TFA to a final concentration of 1% TFA before being loaded on the Sep-Pak Light C18 cartridges (Waters) ...
-
bioRxiv - Immunology 2021Quote: ... plates were coated overnight at 4°C with 15μl purified proteins at optimized concentrations in 0.1M Sodium Bicarbonate: DNA (Worthington, LS002105) at 5μg/mL ...
Product cited:
Highly polymerized; hyperchromicity ≥27%. A substrate for deoxyribonuclease assays. Prepared...Cat# LS002105, 100 mg, $35.00 Ask -
bioRxiv - Cell Biology 2023Quote: ... muscle fibres were digested with collagenase (CLSAFA, Worthington; 1 h; 37 °C), followed by 100 μm and 40 μm cell filtration steps ...
-
bioRxiv - Neuroscience 2021Quote: ... was added to halt the proteolysis and samples were centrifuged at 300xG for 10 minutes at 4°C and resuspended in a 1:1:10 solution of DNase:ovomucoid:Neurobasal media (Worthington:Worthington:Gibco). Cell suspensions were incubated with primary antibodies to CD11b and CD45 (table 1 ...
-
bioRxiv - Immunology 2021Quote: ... Kidneys were digested at 37°C in 1 mg/ml collagenase IV (Worthington) in complete RPMI for 30 minutes ...
-
bioRxiv - Neuroscience 2021Quote: ... was added to halt the proteolysis and samples were centrifuged at 300xG for 10 minutes at 4°C and resuspended in a 1:1:10 solution of DNase:ovomucoid:Neurobasal media (Worthington:Worthington:Gibco). Cell suspensions were incubated with primary antibodies to CD11b and CD45 (table 1 ...
-
bioRxiv - Physiology 2022Quote: Human adipocytes were isolated from adipose tissue samples by collagenase (type 1, Worthington, NJ, USA) as described previously [24] ...
-
bioRxiv - Cancer Biology 2023Quote: ... Pellets were then suspended with 1 ml pre-warmed to 37°C A-mTE-D2 (7 mg ml-1 Dispase II, Worthington NPRO2 ...
-
bioRxiv - Neuroscience 2020Quote: ... and enzymatically digested at 37 °C first using 1 mg/ml Collagenase IV (Worthington, US) in L15 for 25 minutes ...
-
bioRxiv - Biochemistry 2022Quote: ... and digested overnight at 37 °C with 1 µg of trypsin (Worthington, LS003740 TPCK-treated). Samples were then acidified with formic acid (1 % final concentration).
Product cited:
A chromatographically purified, diafiltered, lyophilized powder that has been treated with...Cat# LS003740, 100 mg, $112.00 Ask -
bioRxiv - Biochemistry 2023Quote: ... the particles were incubated at 37°C with 1 μg/μL trypsin TPCK (Worthington-Biochem) for 2 h to induce the proteolysis of BSA and measure in the spectrofluorometer ...
-
bioRxiv - Immunology 2021Quote: ... tissues were digested in Collagenase solution for 1 hour at 37°C (Worthington Biochemical, Lakewood, NJ). Cells were washed once and blocked by Fc Block (Clone ...
-
The cellular basis of protease activated receptor type 2 (PAR2) evoked mechanical and affective painbioRxiv - Neuroscience 2020Quote: ... Ganglia were incubated at 37□C for 25 minutes in 1 mg/ml papain (LS003119; Worthington), followed by 25 minutes of incubation at 37□C in 3 mg/ml collagenase type 2 (LS004176 ...
Product cited:
Supplied as a lyophilized powder prepared from a 2X crystalline suspension, Code: PAP. To...Cat# LS003119, 100 mg, $100.00 Ask -
bioRxiv - Microbiology 2021Quote: ... At pre-determined timepoints post-infection 100μL of PBS (or in the case of influenza virus PBS + 0.1 μg of TPCK-treated trypsin (Worthington, USA)) was added to the apical compartment and cells were incubated at 37°C with 5% CO2 for 10 minutes ...
-
bioRxiv - Cell Biology 2022Quote: Cells were isolated from semitendinosus muscles of 10 Belgian Blue cattle (Supplementary Table 1) by collagenase digestion (CLSAFA, Worthington; 1 h; 37 °C), filtration with 100 μm cell strainers ...
-
bioRxiv - Cancer Biology 2022Quote: ... Human brain tissue was manually minced using razor blades and enzymatically dissociated at 37°C for 30 minutes with 1 mg mL-1 collagenase-4 (#LS004188, Worthington Biochemical Corporation, USA) and 250 U mL-1 DNAse1 (#10104159001 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2022Quote: ... Tissue was then transferred to 1 mL tubes containing papain solution pre-warmed to 37° C (Worthington Biochemical LS003119 ...
-
bioRxiv - Cancer Biology 2023Quote: ... and digested at 37°C for 30 min with 1 mg/mL Collagenase I (Worthington Biochemical, LS004194), 3 mg/mL Dispase II (Roche ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Cancer Biology 2020Quote: ... and digested rotating for 30 minutes at 37°C with 1 mg/mL Collagenase I (Worthington Biochemical LS004194), 3mg/mL Dispase II (Roche 04942078001) ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Cell Biology 2022Quote: ... the subcutaneous inguinal fat pad was isolated bilaterally and digested at 37°C for 1-1.5 hours in 0.2 % collagenase type I (Worthington). Murine ASCs were isolated from digested fat ...
-
bioRxiv - Neuroscience 2020Quote: ... and digested for 1 hour at 37° C in DMEM supplemented with 25 U/ml papain (Worthington Biomedical Corp.), 0.2 mg/ml cysteine (Sigma) ...
-
bioRxiv - Developmental Biology 2022Quote: ... and digested with gentle rocking for 1 h at 37°C in 3 mg/mL collagenase II (Worthington, LS004176) diluted in HBSS containing 5% fetal bovine serum (FBS) ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Bioengineering 2023Quote: Cells were isolated from semitendinosus muscles of Belgian Blue cattle by collagenase digestion (CLSAFA, Worthington; 1 h; 37 °C), filtration with 100 μm cell strainers ...
-
bioRxiv - Neuroscience 2021Quote: ... cerebella from litters of P6 mice were pooled and digested at 37 °C for 20 minutes in 10U ml−1 papain (Worthington), and 250U ml−1 DNase in EBSS using the Papain Dissociation Kit (Worthington ...
-
bioRxiv - Cancer Biology 2021Quote: ... Surgical samples were minced then digested for 1 hour at 37 °C with 2 mg/mL collagenase I (Worthington, #LS004197) and 200 U/mL deoxyribonuclease (Worthington ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004197, 5 gm, $900.00 Ask -
bioRxiv - Cell Biology 2019Quote: ... Kidneys were then cut into small pieces and further dissociated by incubation for 30 min at 37°C in HBSS with 1 mg/ml collagenase (type 2, 255 U/mg, Worthington). To isolate glomeruli ...
-
bioRxiv - Physiology 2020Quote: ... minced with scissors and digested for 1 h at 37°C in 2 mg/ml collagenase type I (Worthington Biochemical) in Krebs-Ringer-HEPES (KRH ...
-
bioRxiv - Physiology 2020Quote: ... muscles were surgically removed and enzymatically dissociated at 37 °C under mild agitation for ~1 h using 1,000 U/mL of collagenase type IV (Worthington Biochemical). Mechanical dissociation was completed using mild trituration in buffer with no collagenase ...
-
bioRxiv - Immunology 2020Quote: ... the aortas were then cut into small pieces (1-2 mm) and incubated for 45 minutes at 37 °C in a type I collagenase (6 mg/ml, Worthington) and elastase (2.5 mg/ml ...
-
bioRxiv - Neuroscience 2020Quote: Primary human cortical tissue was dissociated with Papain (Worthington)-containing DNase ...
-
bioRxiv - Cancer Biology 2021Quote: Tumor-bearing mice were sacrificed and tumors excised and digested at 37°C for 30 min using a cocktail of 1 mg/ml collagenase type IV (Worthington Biochemicals) and 0.02 mg/ml DNase (Sigma-Aldrich ...
-
bioRxiv - Immunology 2019Quote: Tumour bearing mice were sacrificed and tumours were excised and digested at 37°C for 30 min using a cocktail of 1 mg/ml collagenase type IV (Worthington Biochemicals) and 0.02 mg/ml DNase (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2023Quote: Tumor-bearing mice were sacrificed and tumors excised and digested at 37°C for 30 min using a cocktail of 1 mg/ml collagenase type IV (Worthington Biochemicals) and 0.02 mg/ml DNase (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2023Quote: The largest tumors from female C3-TAg and PyMT mice were minced and dissociated for up to 120 min at 37°C in a mixture of 1 mg/ml Collagenase (Worthington, LS004188), 20 U/ml DNase (Invitrogen ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Molecular Biology 2023Quote: ... WAT depots from Scd1fl/fl mice were minced with scissors and digested for 1 h at 37°C in 2 mg/ml collagenase type I (Worthington Biochemical) in Krebs-Ringer-HEPES (KRH ...
-
bioRxiv - Bioengineering 2022Quote: ... and then inflated via the trachea with 2-3 mL of pre-warmed (37°C) enzyme solution (1 mg/mL collagenase/dispase [Roche], 3 U/mL elastase [Worthington], 20 U/mL DNase [Worthington] in DMEM). The heart and large airways were dissected away and the remaining lung tissues and enzyme solution were collected and transferred to a shaking 37°C water bath at 75 rpm for 20 min ...
-
bioRxiv - Systems Biology 2022Quote: ... testes were placed in a 15 ml conical tube containing 2 ml of prewarmed (35 °C) collagenase solution (1 mg/ml type I collagenase (Worthington, cat. # LS004196) and 60 U/ml Turbo Nuclease (Nacalai ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Immunology 2023Quote: ... for 5 min at 4°C to remove circulating cells and then digested in RPMI 1640 with 1 mg/mL Type 2 collagenase (Worthington Biochemical LS004177) for 45 min at 37°C ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Microbiology 2019Quote: ... followed by a 10× dilution using 100 mM Tris-HCl (pH = 8.0) and tryptic digestion under 37°C for 18 hours using 1 µg of trypsin per well (Worthington Biochemical Corp., Lakewood, NJ). Digested peptides were desalted using a panel of lab-made 96-channel filter tips generated by inserting 96 20 µl filter tips into a 96-well cover mat and stacking each filter tip with 5 mg of 10-μm C18 column beads ...
-
bioRxiv - Immunology 2021Quote: ... Collected lobes were incubated for one hour at 37°C in 10 mL of cRPMI with 1500 units of 260 units/mg type 1 collagenase (Worthington Biochemical, Cat. #LS004194) per lobe and pressed through a 35 µm filter ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Microbiology 2021Quote: ... followed by 10X dilution into 50 mM ABC (pH 8.0) and tryptic digestion at 37°C for 20 hr with shaking using 1 μg of trypsin (Worthington Biochemical Corp., Lakewood, NJ) per 50 μg protein ...