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Citations for Worthington Biochemical :
101 - 150 of 155 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... calvariae were collected from 3 days old mice and washed with 1× PBS and digested 2 times for 10 minutes in digestion solution (αMEM, 0.1 mg/ml collagenase type 2 [Worthington Biochemical Corporation] and 0.00075% trypsin ...
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bioRxiv - Immunology 2020Quote: ... minced tissue was resuspended in 10ml GKN + 10% fetal calf serum (FCS; Serana) with collagenase IV (Worthington Biochemical Corp.) and DNase (Sigma-Aldrich ...
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bioRxiv - Pathology 2022Quote: ... tissue was enzymatically digested in digestion buffer (0.5 g of fat in 10 ml) including 0.8 mg/ml of collagenase II (Worthington Biochemicals), 3% of BSA (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2022Quote: ... Fresh schwannomas were minced with sterile Bard-Parker #10 surgical scalpels (Aspen Surgical) and incubated in 0.4% Collagenase Type 2 (Worthington) in pre-oxygenated Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: Tumor cell suspensions were prepared by digestion of finely minced tissue for 30 min at 37 °C using DMEM 10% FCS supplemented with 1.5 mg/ml Collagenase IV (Worthington) and 50 U/ml DNAse I (Roche) ...
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bioRxiv - Immunology 2023Quote: ... Cells were then DNase treated with 4 ml disaggregation media (DMEM, 10% FBS, 100 μg/ml DNASE I (Worthington)) for 20 min at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... Samples were incubated during 10 min at RT with 0.1 mg/ml of DNAse I (PN LS002007, Worthington-Biochem) in order to eliminate ambient DNA and favor the resuspension of the pellet ...
Product cited:
Chromatographically purified. A lyophilized powder with glycine as a stabilizer.Cat# LS002007, 100 mg, $350.00 Ask -
bioRxiv - Genetics 2020Quote: ... supplemented with 10 μM Rock inhibitor and 33 μg/mL DNase I (Worthington, LK003170, only for days 30 and 52). The cells were dissociated using a P1000 and collected in a 15 mL tube capped with a 40 μm cell strainer ...
Product cited:
A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered...Cat# LK003170, 1 vi, $28.00 Ask -
bioRxiv - Immunology 2019Quote: ... DRGs were further digested with 1 mL of collagenase type II/dispase solution (HBSS/10 mM HEPES/4 mg/mL collagenase type II (Worthington)/5 mg/mL dispase (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... tissue disaggregation was performed by finely chopping liver samples (~1-2 g) in 10 ml digestion solution containing 1 mg/ml Collagenase IV (Worthington) and 20 μg/ml DNAse1 (Roche ...
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bioRxiv - Bioengineering 2020Quote: ... Cell suspensions were harvested by mechanical dissection and Collagenase IV Cocktail digestion (formulated as 7.4 kU Collagenase Type 4, 53 kU Deoxyribonuclease I, 20 mg Soybean Trypsin Inhibitor in 10 mL cocktail; Worthington Biochemical). The suspensions were passed through 40 μm cell strainers to ensure singularity ...
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bioRxiv - Microbiology 2020Quote: ... All viruses were titrated using 1:10 serial dilution in triplicate in LLCMK2 cells in the presence of 2μg/mL TPCK trypsin (Worthington Biomedical) for 72 hours and TCID50/mL was determined by the Reed-Meunch method based on hemagglutination of 0.5% washed chicken red blood cells (Lampire ...
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... The tissue was then titurated in a digestion media containing 10 U/ml elastase (#LS002280, Worthington Biochemical, Lakewood, NJ, USA) and incubated for 30 minutes at 37°C ...
Product cited:
Two times crystallized. Supplied as an aqueous suspension. This preparation must be diluted to...Cat# LS002280, 1 gm, $1065.00 Ask -
bioRxiv - Developmental Biology 2019Quote: Isolated mouse embryos were minced and digested in sorting medium (Ham’s F10 with 10% horse serum) containing 400U/ml Collagenase II (Worthington; LS004177) for 1h at 37 °C in a shaking water bath ...
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bioRxiv - Neuroscience 2019Quote: ... Retinae were rapidly dissected from the eyecups and incubated at 37 °C for 30 min in a digestion solution containing papain (10 U/mL; Worthington) and L-cysteine (0.2 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... Lungs were instilled with either 1 mL of 10% formalin for histology studies or 1 mL of digestion buffer (90%DMEM 10% PBS+Calcium with 180 U/mL Type 2 Collagenase (Worthington) and 15 μg/mL DNase (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were then incubated at 37°C for 3 minutes in a solution of HBSS/0.2% glucose with 10 mg/ml trypsin (Worthington Biochemical) and 1 mg/ml Deoxyribonuclease I (Worthington Biochemical) ...
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bioRxiv - Neuroscience 2023Quote: ... Cortical tissues were isolated from P0 mouse brains and digested with 10 U/mL papain (Worthington Biochemical Corp. Lakewood, NJ). Mouse cortical neurons were plated on following poly lysine-coated glass bottom dishes (500,000 cells ...
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bioRxiv - Developmental Biology 2022Quote: ... 300 μL Dulbecco’s Modified Eagle’s Medium (DMEM) (ATCC 30-2002) and 150 μL of 10 mg/mL collagenase Type II (Worthington LS004176) were added to each brain and incubated for 5-10 min at 37 °C ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Physiology 2023Quote: ... Hearts were then perfused for 5 - 10 min with 0.125 mM CaCl2 KRH buffer supplemented with 1mg/mL of type II collagenase (Worthington Biochemical). Ventricles were cut into 0.1 mM CaCl2 solution ...
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bioRxiv - Immunology 2023Quote: ... The right lobes of the lungs were collected in 5 mL of digestion buffer (90% DMEM and 10% PBS and calcium with type 2 collagenase (180 U/ml) (Worthington) and DNase (15μg/ml ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Ganglia were cut into small pieces and cells were dissociated by incubating ganglia pieces in enzyme solution (10 mg/mL collagenase IV (Worthington), in 1XHBS (HEPES buffered saline ...
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bioRxiv - Cell Biology 2023Quote: ... The minced muscles were centrifuged at 15,000 x g for 1 minute and transferred to 10 ml of digestion buffer (DMEM containing 2.5% HEPES, 1% penicillin/streptomycin, and 400 U/ml collagenase II (Worthington Biochemical)) and placed on an orbital shaker at 100 rpm for 1 hour at 37°C ...
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bioRxiv - Genomics 2021Quote: ... Day 10 CRISPRi neurons cultured in the +AO medium and -AO medium were dissociated using Papain (Worthington; Code: PAP2; Cat. No.LK003178) and stained by CellRox Green (Invitrogen/Thermo Fisher Scientific ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Genomics 2020Quote: ... Day 10 (HH35-36) and day 14 (HH40) ventricular samples were digested in 300U/mL collagenase type II (Worthington Biochemical Corporation) for four cycles of 10 minutes under mild agitation at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... The digestion was quenched by adding dissociation media supplemented with 0.2% BSA and 10 mg/ml ovomucoid inhibitor (Worthington Cat. # LK003128), and samples were kept chilled for the rest of the dissociation procedure ...
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bioRxiv - Neuroscience 2019Quote: ... The digestion was quenched by adding dissociation media supplemented with 0.2% BSA and 10 mg/ml ovomucoid inhibitor (Worthington Cat. # LK003128), and samples were kept chilled for the rest of the dissociation procedure ...
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bioRxiv - Neuroscience 2021Quote: ... Hippocampi were dissected in ice-cold Hank’s balanced salts solution (HBSS) and kept on ice until digestion with 0.2 μm-filtered 10 U/mL papain (Worthington Biochemical Corporation) in HBSS in a 37°C water bath for 20 min ...
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bioRxiv - Cell Biology 2022Quote: ... ears of wild-type C57BL/6J mice were collected and mechanically disrupted before enzymatic dissociation in 10 mg/ml Collagenase IV (Worthington, LS004183), 0.2% FBS and 0.2 mg/ml DNase I (Worthington ...
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bioRxiv - Neuroscience 2019Quote: ... 0.16mM NaOH pH=7.4) that contained 10 U/mL papain and 5mg of L-cysteine in total (Worthington, Lakewood, NJ, USA) for 10 minutes at 37°C ...
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bioRxiv - Immunology 2021Quote: CHO cells stably expressing wild-type SARS-CoV-2 S were resuspended in wash buffer (PBS 1 % BSA, 2 mM EDTA) and treated with 10 µg/mL TPCK-trypsin (Worthington Biochem) for 30 min at 37°C ...
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bioRxiv - Immunology 2020Quote: ... and both fragments were separately digested in a petri-dish at 37°C 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (12 hours) ...
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bioRxiv - Cancer Biology 2022Quote: ... which included a first step of PDX tumor mechanical dissociation with sterile scalpels and then enzymatic dissociation by trypsin (10 mg/ml) and type II collagenase (275 U/mg, Worthington Biochemical). The tube was then placed in a warm 37°C water bath for 60 min ...
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bioRxiv - Neuroscience 2023Quote: ... Hippocampi were isolated from P0 CD-1 mouse brain tissues and digested with 10 U/mL papain (Worthington Biochemical Corp., LK003176). Mouse hippocampal neurons were plated on following poly lysine-coated dishes for each experiment - glass bottom dishes (500,000 cells ...
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bioRxiv - Cell Biology 2023Quote: ... the tissues were further transferred into 20 mL of pre-warmed digestion buffer (RPMI medium containing 10% FBS, 1% P/S, 15 mM HEPES, 25 U/mL of collagenase IV (Worthington LS004186), 100 U/ml of DNase I (Sigma D4513) ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Bioengineering 2023Quote: ... hACs were isolated using 10 ml of a 0.15% (w/v) type II collagenase (300 U mg−1; Worthington Biochemical Corporation) solution per g of tissue (37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... The tissue was then washed with PBS and digested for 10 min at 37°C in 3 mg/ml collagenase II (Worthington, LS004176) in HBSS supplemented with 5% FBS and 10 mM HEPES ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Genomics 2023Quote: ... Each sample was centrifuged at 1200 rpm x5 mins at 4°C and resuspended in 10 mL warmed digestive media (HBSS + 1% HEPES) with 1,000 U/mL collagenase type IV (Worthington, Cat: LS004188), and rotated for 30 min at 37 °C ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Cortices were isolated from P0 CD-1 mouse brain tissues and digested with 10 U/mL papain (Worthington Biochemical Corp., LK003176). Mouse cortical neurons were plated on following poly lysine-coated glass bottom dishes (500,000 cells) ...
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bioRxiv - Bioengineering 2024Quote: ... hippocampi were dissected from brains of newborn pups (P0) and digested for 20 min at 37 °C with 10 units of papain (Worthington, USA), followed by gentle mechanical trituration ...
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bioRxiv - Immunology 2024Quote: ... mice were perfused with 1X PBS and the CNS was isolated into 10 mL of enzyme digestion solution consisting of 75 µL Papain suspension (Worthington, #LS003126) diluted in enzyme stock solution (ESS ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Bioengineering 2021Quote: The elastin degraded model was produced by incubating native porcine carotid arteries in 10 U/ml purified elastase (ESFF, Worthington Biochemical Corporation) with 0.35 mg/ml trypsin inhibitor (10109886001 ...
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bioRxiv - Immunology 2021Quote: ... lungs were minced with a scalpel and resuspended in 10ml GKN + 10% fetal calf serum (FCS) with collagenase IV (Worthington Biochemical Corp.) and DNase (Sigma-Aldrich ...
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Acinar-to-ductal metaplasia in the pancreas requires a glycolytic switch and functional mitochondriabioRxiv - Cancer Biology 2022Quote: ... specimens were cut into pieces and digested twice with 10 ml/g tissue of 50 U/ml collagenase (CLSPA Worthington Biochemical Corporation) in SOL1 for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... Retinas were detached from the retinal pigmented epithelium and incubated for 15 min at 37°C in a solution containing papain (10 U/ml, Worthington; Lakewood, NJ) and L-Cysteine hydrochloride (∼1 mg/mL ...
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bioRxiv - Immunology 2021Quote: ... Collected lobes were incubated for one hour at 37°C in 10 mL of cRPMI with 1500 units of 260 units/mg type 1 collagenase (Worthington Biochemical, Cat. #LS004194) per lobe and pressed through a 35 µm filter ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Cell Biology 2022Quote: Cells were isolated from semitendinosus muscles of 10 Belgian Blue cattle (Supplementary Table 1) by collagenase digestion (CLSAFA, Worthington; 1 h; 37 °C), filtration with 100 μm cell strainers ...
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bioRxiv - Microbiology 2022Quote: ... with 10% fetal calf serum and antibiotics/antimycotics supplemented with 1g/ml tosyl phenylalanyl chloromethyl ketone (TPCK)-trypsin (Worthington Biochemical Corp., Lakewood, NJ).
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bioRxiv - Cancer Biology 2023Quote: ... spun down by centrifugation at 500 x g for 5 minutes and resuspended in 10 ml of digestion medium (MEM containing 112 U/mL type I collagenase; Worthington, cat. #CLS-1), 1.2 U/mL dispase (Gibco ...
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bioRxiv - Immunology 2024Quote: ... and then incubated in 5 mL digestion buffer (basal media, 10 µM Y-27632, 600 U/mL Collagenase IV (Worthington cat. No. LS004189), 0.1 mg/mL DNAse I (Sigma-Aldrich ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004189, 5 gm, $900.00 Ask