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Citations for Worthington Biochemical :
401 - 450 of 786 citations for 5 1 3 Dioxolan 2 yl 2 3 fluorobenzoyl thiophene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Mice were immunized as indicated in each Figure with 5 µg low-endotoxin OVA (Worthington Biochemical Corporation) mixed with either 50 µg papain (P4762 ...
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bioRxiv - Neuroscience 2024Quote: ... Dissociation medium was aspirated until 1 mL remained and an activated papain solution (1:1 papain [Worthington-Biochem] with 13.6 mM Cysteine-HCL ...
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bioRxiv - Cancer Biology 2021Quote: ... Right lung lobes were minced and digested with 5 mL digestion buffer: Collagenase 4 (200U/mL, Worthington Biochemicals), DNase I (100 ug/mL ...
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bioRxiv - Cell Biology 2023Quote: ... Half of the samples were treated with a final concentration of 5 μg/mL bacterial collagenase (Worthington Biochemical) for 3 d at 37 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... in 1:1 DMEM:F12K supplemented with DNase I (Worthington Biochemical), 6.5mg/ml collagenase (Worthington Biochemical) ...
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bioRxiv - Developmental Biology 2024Quote: ... supplemented with 0.3 mg/ml Collagenase Type 1 (Worthington, CLS-1) and 10 mg/ml DNase I (Sigma ...
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bioRxiv - Physiology 2021Quote: ... The proteins were digested for 5 hours with Lys-C (Wako) at room temperature and overnight with trypsin (Worthington). Samples were acidified and diluted with TFA to a final concentration of 1% TFA before being loaded on the Sep-Pak Light C18 cartridges (Waters) ...
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bioRxiv - Neuroscience 2021Quote: ... pH adjusted to 7.6] and mechanically dissociated to single cells after adding ovomucoid (Worthington, 0.7 mg per 5 mice) and DNAse (Worthington ...
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in Dispase II (5 mg/mL; StemCell 7913) and DNase I (100 μg/mL; Worthington Biochemical LS002006) for 5 minutes ...
Product cited:
Chromatographically purified. A lyophilized powder with glycine as a stabilizer.Cat# LS002006, 20 mg, $96.00 Ask -
bioRxiv - Cell Biology 2020Quote: ... lungs were perfused with 5 ml PBS and then digested with 4 U/ml elastase (Worthington Biochemical Corporation, NJ) and 100 U/ml DNase I (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... cells were centrifuged for 5 minutes and the pellet was resuspended with DNAase/ovomucoid inhibitor according to manufacturer’s protocol (Worthington). Cells were then centrifuged and resuspended on ice in Leibovit’z L-15 medium supplemented with 0.1% of bovine serum albumin ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryonic single cell suspensions from were obtained by performing eight rounds of enzymatic digestion (5 minutes each) with Collagenase type II (0.7mg/ml Worthington) at 37°C under agitation ...
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bioRxiv - Immunology 2023Quote: ... 15 mL of pre-warmed digestion solution (1X HBSS with Ca+ and Mg, 5% FBS, 50 U/ml Dispase II [Millipore-Sigma, cat # 4942078001], 0.5 mg/ml DNAse I [Worthington Biochemical ...
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Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: ... The nuclei suspension was preincubated at 37°C for 5 min and followed by micrococcal nuclease (Worthington, cat# LS004798) digestion was carried out by following the manufacturer’s protocol ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004798, 45 ku, $211.00 Ask -
bioRxiv - Physiology 2023Quote: ... The muscle layer was cut into pieces and incubated in 5 ml of digestive solution containing 1.5 mg collagenase (Worthington), 1 mg trypsin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... The remaining tissue was then incubated in 5 mL of digest solution [200 U/mL Collagenase IV (Worthington, LS004189) and 400 U/mL DNase I (Worthington ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004189, 5 gm, $900.00 Ask -
bioRxiv - Immunology 2024Quote: ... cut into small ∼1mm3 pieces and enzymatically digested in DMEM containing 5 mg/mL type II collagenase (Worthington, # LS004202) and 0.2 mg/mL DNase I (Roche ...
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bioRxiv - Neuroscience 2020Quote: ... prepared using 1:1 albumin-ovomucoid solution (10mg/ml of each) (Worthington, Cat# OI ...
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bioRxiv - Cancer Biology 2024Quote: ... containing 1 mg/ml each of Collagenase Type 1 (Worthington Biochemical LS004216), Type 2 (Worthington Biochemical LS004204) ...
Product cited:
Collagenase, Type 1 (Code: CLS-1), which is filtered through a 0.22 micron membrane and...Cat# LS004216, 5x50 mg, $200.00 Ask -
bioRxiv - Genomics 2023Quote: ... for 1 hour (Patient #1) or 3.7mg/mL collagenase II (Worthington, LS004177) for 90 minutes followed by 6mg/mL dispase (Gibco ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... and 1% DNase (Worthington) at 37°C for 6 minutes at which point DMEM (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... 100-200 mg of tumor bearing lung tissue was then thoroughly minced with Noyes scissors, prior to incubation with digestion buffer (HBSS supplemented with 5% HI FBS, 125 U/mL collagenase IV (Worthington) and DNase (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... and 5 mM MgCl2) and LC-MS/MS was performed by on-bead digestion with trypsin (TPCK-treated, Worthington Biochemical).
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Harvest and Culture of Porcine Adipose-Derived Mesenchymal Stem Cells for Autologous TransplantationbioRxiv - Cell Biology 2020Quote: ... (Fat Processing Video) Phosphate-buffered saline (PBS; 100 mL) was mixed with 75 mg (±5 mg) of type A collagenase (Worthington Biochemical Corporation ...
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bioRxiv - Immunology 2020Quote: ... Approximately 1cm2 of skin was digested overnight in 5%CO2 at 37°C with 3ml of digestion mix containing 0.8mg/ml Collagenase Type 4 (Worthington; #LS004186) and 0.02mg/ml DNase (Sigma-Aldrich ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Cancer Biology 2022Quote: ... Tumor pieces were chopped and incubated 5 min at 37°C in a HBSS-papain based lysis buffer (Worthington PAP) containing DNAse (0.01% ...
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bioRxiv - Physiology 2023Quote: ... Homogenate was digested with liver digestion buffer (5 ml HBSS w/o Ca2+/Mg2+, 200U/ml Collagenase IV (Worthington, USA) (37 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... at 4°C followed by eight rounds of enzymatic digestion (5 minutes each) with Collagenase type II (1mg/ml Worthington) at 37°C ...
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bioRxiv - Physiology 2023Quote: ... Hearts were then perfused for 5 - 10 min with 0.125 mM CaCl2 KRH buffer supplemented with 1mg/mL of type II collagenase (Worthington Biochemical). Ventricles were cut into 0.1 mM CaCl2 solution ...
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bioRxiv - Cancer Biology 2024Quote: ... The material was then transferred to a 5 mL microcentrifuge tube containing 2.5 mL Papain supplemented with 125 uL DNase (Worthington LK003150) and incubated for 30-45 minutes in a 37°C incubator ...
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bioRxiv - Cancer Biology 2024Quote: ... the tissue was enzymatically and mechanically digested for 5-10 minutes at 37°C in HBSS-papain-based lysis buffer (Worthington) containing DNAse (0.01% ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary tumor and metastasized lung tissues were digested in 5 mL of enzyme buffer containing 0.2 mg/mL collagenase type-II (Worthington, LS004174), 0.1 mg/mL DNase I (Roche ...
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bioRxiv - Biochemistry 2024Quote: ... Lungs from 4 mice were minced into ∼1 mm pieces using curved scissors and digested with 1 mg/ml collagenase type 1 (Worthington, cat# LS004194) and 1X dispase (Corning ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... SNc regions from the slices were dissected out with a scalpel blade and digested with oxygenated high glucose HEPES-buffered saline containing papain (5–8 U/ml, Worthington, USA) for 20–30 min at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... the dissociation buffer was warmed to 34□ and 5 mL was added to a vial of lyophilized papain (Worthington Biochemical Corporation). The solution was diluted 1:2 with additional dissociation buffer prior to sample dissociation.
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bioRxiv - Molecular Biology 2022Quote: ... Primary osteoblasts were isolated from the calvaria of 5-day-old mouse pups using collagenase type II (50 mg/ml, Worthington, LS004176)/dispase II (100 mg/ml ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Physiology 2020Quote: ... This was followed by a wash step and centrifugation at 500 x g for 5 min before removing the dissociation buffer and adding stock collagenase II (4.3 mg/ml) (Worthington, Lakewood, USA) and stock dispase (6.0 mg/ml ...
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bioRxiv - Immunology 2020Quote: ... and both fragments were separately digested in a petri-dish at 37°C 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (12 hours) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Primary calvarial osteoblasts were isolated from calvaria of 5-day-old neonates using collagenase type II (50 mg/ml, Worthington, LS004176)/dispase II (100 mg/ml ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2024Quote: Cortical assembloids were washed with 1X PBS and dissociated at 37°C 5% CO2 on an orbital shaker with Papain (Worthington #LK003150) for 30 minutes ...
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bioRxiv - Synthetic Biology 2024Quote: ... and both fragments were separately digested in a petri dish at 37°C 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) for 12 hours ...
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bioRxiv - Genetics 2020Quote: ... 1 mg ml-1 soybean trypsin inhibitor (#LS003570, all purchased from Worthington Biochemical Corporation), and 1% penicillin/streptomycin in HBSS for 10 min at 37°C with gentle shaking ...
Product cited:
Chromatographically purified as described by Frattali and Steiner, Biochem., 7, 521 (1968). A...Cat# LS003570, 100 mg, $74.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... papain (1 mg/ml, Worthington), ethylenediaminetetraacetic acid (EDTA ...
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bioRxiv - Immunology 2023Quote: ... 1 mg/ml hyaluronidase (Worthington), 0.1 mg/ml DNase I (Roche ...
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bioRxiv - Genomics 2022Quote: ... Nuclei were collected by centrifugation and DNase I-treated for 30 min at 37°C (Worthington, 30U per 5 x 106 cells). After DNase I digestion ...
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bioRxiv - Microbiology 2022Quote: ... Cultures were incubated statically at 37°C with 5% CO2 followed by plating on TSA plates supplemented with 100 µl of catalase (38,000 U/ml; Worthington Biochemical Corporation, NJ) and the desired antibiotic (250 µg/ml kanamycin or 200 µg/ml streptomycin) ...
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bioRxiv - Molecular Biology 2022Quote: Five 10um cryostat slices of patient polyps were digested for 5 min at 37°C with a titration of MNase: 20 units/mL and 200 unit/mL of MNase (Worthington Biochemical Corp.) in MNase cleavage buffer (4 mM MgCl2 ...
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bioRxiv - Neuroscience 2023Quote: ... washed twice in HBSS and incubated during 30 min at 37°C in pre-warmed, oxygenated (95% O2, 5% CO2) Papain/DNase I (Worthington Biochemical Corporation) on a gyratory shaker (100 rpm) ...
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bioRxiv - Neuroscience 2023Quote: ... This solution was then replaced with 1mg/ml collagenase 1 (Worthington Biochemical Corporation CLS-1) in 0.1M PBS and 20mM HEPES for 60-90 min at 37°C followed by 0.05% Trypsin-EDTA (Sigma T9201 ...
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bioRxiv - Bioengineering 2024Quote: ... Tissue was minced until 1mm pieces remained, then lysed in equilibrated (37°C, 5% CO2) enzyme (20 U/mL Papain (Worthington Biochemical Corp; LK003176) in 5mL of EBSS (Sigma ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask