Labshake search
Citations for Worthington Biochemical :
1351 - 1400 of 1528 citations for Mono 2 Carboxymethyl Hexyl Phthalate Cp 95% 13C4 99% Dehp Metabolite Iv 100Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... cerebella from litters of P6 mice were pooled and digested at 37 °C for 20 minutes in 10U ml−1 papain (Worthington), and 250U ml−1 DNase in EBSS using the Papain Dissociation Kit (Worthington ...
-
bioRxiv - Genetics 2020Quote: ... supplemented with 10 μM Rock inhibitor and 33 μg/mL DNase I (Worthington, LK003170, only for days 30 and 52). The cells were dissociated using a P1000 and collected in a 15 mL tube capped with a 40 μm cell strainer ...
Product cited:
A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered...Cat# LK003170, 1 vi, $28.00 Ask -
bioRxiv - Molecular Biology 2019Quote: ... of 0.1 focus-forming unit (FFU)/cell in M199 medium supplemented with 1 mg/mL porcine pancreatic trypsin (Worthington Biochemical). Cell culture medium was collected 24–36 h post infection ...
-
Pluripotent Stem Cells Can Be Isolated from Human Peripheral Nerves after in vitro BMP-2 StimulationbioRxiv - Cell Biology 2019Quote: ... the minced nerve pieces were pelleted by centrifugation at 500xg for 5 minutes and followed by digestion in 0.2% (0.27 U/ml) collagenase (Worthington Biochemical Corp) at 37°C for 90 minutes ...
-
bioRxiv - Neuroscience 2019Quote: ... The cells were then centrifuged at 300*g for 5 minutes and the pellet resuspended in 1 mL of trypsin inhibitor (Worthington) plus DNAse (Sigma ...
-
Harvest and Culture of Porcine Adipose-Derived Mesenchymal Stem Cells for Autologous TransplantationbioRxiv - Cell Biology 2020Quote: ... (Fat Processing Video) Phosphate-buffered saline (PBS; 100 mL) was mixed with 75 mg (±5 mg) of type A collagenase (Worthington Biochemical Corporation ...
-
bioRxiv - Bioengineering 2020Quote: ... Cell suspensions were harvested by mechanical dissection and Collagenase IV Cocktail digestion (formulated as 7.4 kU Collagenase Type 4, 53 kU Deoxyribonuclease I, 20 mg Soybean Trypsin Inhibitor in 10 mL cocktail; Worthington Biochemical). The suspensions were passed through 40 μm cell strainers to ensure singularity ...
-
bioRxiv - Bioengineering 2020Quote: ... The dissected strips of HTM tissue were digested for 20 min with 1 mg/ml collagenase (Worthington, Lakewood, NJ, USA) and 15 μl/ml human albumin (Sigma-Aldrich ...
-
bioRxiv - Physiology 2021Quote: ... The arteries were then washed three times with Mg2+-PSS and incubated in 1 mg/mL collagenase type II (Worthington) in Mg2+-PSS for 14 minutes ...
-
bioRxiv - Plant Biology 2021Quote: ... Samples were incubated at 37°C for one hour followed by adding 1.5 μg/mL trypsin inhibitor (Worthington Biochemical. Corp) to inactivate trypsin ...
-
bioRxiv - Microbiology 2020Quote: ... All viruses were titrated using 1:10 serial dilution in triplicate in LLCMK2 cells in the presence of 2μg/mL TPCK trypsin (Worthington Biomedical) for 72 hours and TCID50/mL was determined by the Reed-Meunch method based on hemagglutination of 0.5% washed chicken red blood cells (Lampire ...
-
bioRxiv - Microbiology 2022Quote: ... 18-24hrs post transfection, the media was replaced with viral growth media (MEM, 1 mM HEPES, 1 μg/mL TPCK trypsin (Worthington Biochemical Corporation ...
-
bioRxiv - Bioengineering 2022Quote: ... The distal lung tissue was separated from the mainstem bronchi and transferred to a Petri dish containing DMEM with 1% HEPES and 100 U/mL DNase I (Worthington). The tissue was then dissociated with a blunt bone marrow aspiration needle ...
-
bioRxiv - Microbiology 2021Quote: ... 0.1% Bovine Serum Albumin (BSA) (MP Biomedical) and 1 µg/ml of TPCK (tolylsulfonyl phenylalanyl chloromethyl ketone)-trypsin (Worthington Biomedical) (Weingartl et al. ...
-
bioRxiv - Microbiology 2020Quote: ... Spn were grown on tryptic soy (TS; Becton Dickinson) agar plates supplemented with 100 μl of catalase (30,000 U/ml; Worthington Biomedical) and appropriate antibiotics (200 μg/ml streptomycin ...
-
bioRxiv - Physiology 2022Quote: Digestion of the left kidney was performed by a 20 minutes-incubation in HBSS containing type II collagenase at 0.5 mg/ml (Worthington, USA), followed by a 4 minutes-incubation in trypsin 0.04% at 37°C after removal of the capsule ...
-
bioRxiv - Developmental Biology 2022Quote: ... tissue was washed twice with 0.3ml ice- cold CMF PBS and incubated in 0.3ml papain solution (20U/ml, 1mM L-Cysteine and 0.5mM EDTA; Worthington Biochemical Corporation) at 37°C for 40 minutes ...
-
bioRxiv - Molecular Biology 2020Quote: ... The remaining cut tissue sections were weighed and incubated with 50 U/mL collagenase type 3 (#CLS-3, CAT#LS004182, Worthington) digestion buffer (at ratio of 8μL / mg tissue ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004182, 1 gm, $215.00 Ask -
bioRxiv - Bioengineering 2022Quote: ... cells showed spontaneous beating and were dissociated on day 15 by incubation with 280 U/ml Collagenase (LS004174, Worthington, USA) and 40 U/ml DNase (LS006331 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004174, 100 mg, $42.00 Ask -
bioRxiv - Immunology 2020Quote: ... Approximately 1cm2 of skin was digested overnight in 5%CO2 at 37°C with 3ml of digestion mix containing 0.8mg/ml Collagenase Type 4 (Worthington; #LS004186) and 0.02mg/ml DNase (Sigma-Aldrich ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... The tissue was then titurated in a digestion media containing 10 U/ml elastase (#LS002280, Worthington Biochemical, Lakewood, NJ, USA) and incubated for 30 minutes at 37°C ...
Product cited:
Two times crystallized. Supplied as an aqueous suspension. This preparation must be diluted to...Cat# LS002280, 1 gm, $1065.00 Ask -
bioRxiv - Immunology 2022Quote: ... which consisted of HBSS containing Ca2+ and Mg2+ supplemented with 3.4 mg/ml collagenase type 1 (Worthington Biochemical Corporation LS004196) and 100 U/ml hyaluronidase (Sigma-Aldrich H3631) ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Developmental Biology 2020Quote: ... Ventricles of three hearts were micro-dissected and pooled into a 600 μl mix consisting of 1.2 U/ml dispase (Worthington #LS02100), 500 U/ml collagenase IV (Worthington #LS004186) ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02100, 10 mg, $84.00 Ask -
bioRxiv - Immunology 2019Quote: ... Approximately 1cm2 of skin was digested overnight in 5%CO2 at 37°C with 3ml of digestion mix containing 0.8mg/ml Collagenase Type 4 (Worthington; #LS004186) and 0.02mg/ml DNase (Sigma-Aldrich ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Developmental Biology 2019Quote: Isolated mouse embryos were minced and digested in sorting medium (Ham’s F10 with 10% horse serum) containing 400U/ml Collagenase II (Worthington; LS004177) for 1h at 37 °C in a shaking water bath ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Physiology 2022Quote: ... before perfusion was switched to EGTA-free isolation buffer supplemented with 1 mg/mL collagenase (type II; Worthington Biochemical, USA), 0.06 mg/mL protease XIV ...
-
bioRxiv - Cell Biology 2019Quote: ... the heart was moved to a dish containing 475 U mL-1 collagenase type II (Worthington Biochemical Corporation, Lakewood NJ) in perfusion buffer with 15 µmol L-1 blebbistatin ...
-
bioRxiv - Neuroscience 2019Quote: ... Retinae were rapidly dissected from the eyecups and incubated at 37 °C for 30 min in a digestion solution containing papain (10 U/mL; Worthington) and L-cysteine (0.2 μg/mL ...
-
bioRxiv - Microbiology 2020Quote: ... 1 hour after infection media was changed to serum-free media supplemented with 1µg/ml tosyl phenylalanyl chloromethyl ketone (TPCK)-treated trypsin (Worthington-Biochemical). Passage experiments were performed by infecting cells at an MOI of 0.01 ...
-
bioRxiv - Physiology 2019Quote: ... consisting of the aforementioned solution supplemented with 50 μM Ca2+ and collagenase type II (1 mg/mL; Worthington, Allschwil, Switzerland). Subsequently ...
-
bioRxiv - Microbiology 2019Quote: ... was propagated in MA104 cells in serum-free DMEM in the presence of 1 µg/mL Worthington’s Trypsin (Worthington Biochemical). After harvest ...
-
bioRxiv - Cell Biology 2019Quote: ... The muscles were digested in 4 ml:1 g of tissue wet weight in a solution of 0.5% Collagenase II (Worthington Biochemicals), 1.2 U/ml Dispase (Worthington Biochemicals) ...
-
bioRxiv - Cell Biology 2019Quote: ... the dermis was finely diced and incubated with 400 U/ml of collagenase type I (Worthington Biochemical, Lakewood, NJ, USA) for 30 minutes at 37 °C ...
-
bioRxiv - Neuroscience 2022Quote: ... Pups were decapitated and hippocampi were dissected and incubated in dissociation solution (7 mL HBSS buffered saline, 150 μL 100mM CaCl2, 10 μL 1M NaOH, 10 μL 500mM EDTA, 200 units Papain [Worthington]) at 25°C for 1 h (rat ...
-
bioRxiv - Developmental Biology 2021Quote: ... and 10 pooled coronal sutures were rinsed in PBS and enzymatically dissociated with a final concentration of 3 mg/mL of Collagenase II (Worthington) and 4 units/mL of Dispase (Corning ...
-
bioRxiv - Genomics 2020Quote: ... After removal of the epidermal sheets, the dermis was finely minced and enzymatically digested (RPMI basal medium, 1000U/mL collagenase type 1 (Worthington), 40µg/mL DNase I (Roche) ...
-
bioRxiv - Developmental Biology 2022Quote: ... The individually isolated spinal cords were digested at 37°C for 8 min in 0.25 mg/ml of trypsin (Worthington Biochemical), and the digestion stopped with 10x trypsin-inhibitor (Sigma) ...
-
bioRxiv - Cell Biology 2022Quote: ... Mouse hindlimb muscles were dissected and incubated in digestion media (2.5 U/mL Dispase II; Thermo and 0.2% w/v Collagenase Type II; Worthington in DMEM) on a shaking incubator at 37°C for 1.5 hours ...
-
bioRxiv - Developmental Biology 2022Quote: Hearts from E14.5 embryos were isolated and incubated 30 minutes at 37°C on a rotating shaker with 200 µl of digestion solution per embryo consisting out of 0.05 mg/ml DNase I (Worthington, 2139) and 1 mg/ml Collagenase I (Worthington ...
-
bioRxiv - Cell Biology 2022Quote: Following administration of tamoxifen extensor digitorum longus (EDL) muscles were isolated from 8-12 week-old mice and incubated in Collagenase I (2mg/ml, Worthington,) for 90 minutes at 37°C ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... using a scalpel and surgical scissors and directly placed into 2mL of enzymatic solution: Collagenase II – 500U/mL (WOLS04177, Worthington), DNase I – 1,6U/mL (11284932001 ...
-
bioRxiv - Neuroscience 2022Quote: ... Retinas were washed 3 times for 3 min in L-15 solution and then placed in L-15 solution containing 7 U ml-1 papain (cat. no. 3126, Worthington Biochemical Corporation ...
-
bioRxiv - Neuroscience 2022Quote: ... Tissues were then incubated at 37°C for 3 minutes in a solution of HBSS/0.2% glucose with 10 mg/ml trypsin (Worthington Biochemical) and 1 mg/ml Deoxyribonuclease I (Worthington Biochemical) ...
-
bioRxiv - Developmental Biology 2022Quote: ... at 4°C followed by eight rounds of enzymatic digestion (5 minutes each) with Collagenase type II (1mg/ml Worthington) at 37°C ...
-
bioRxiv - Neuroscience 2023Quote: ... Cortical tissues were isolated from P0 mouse brains and digested with 10 U/mL papain (Worthington Biochemical Corp. Lakewood, NJ). Mouse cortical neurons were plated on following poly lysine-coated glass bottom dishes (500,000 cells ...
-
bioRxiv - Developmental Biology 2022Quote: ... 300 μL Dulbecco’s Modified Eagle’s Medium (DMEM) (ATCC 30-2002) and 150 μL of 10 mg/mL collagenase Type II (Worthington LS004176) were added to each brain and incubated for 5-10 min at 37 °C ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Immunology 2022Quote: ... Aortae were dissected and digested in a solution containing of 1200 U/ml collagenase II (Worthington Biochemicals, Lakewood, NJ, USA), 60 U/ml DNase (Sigma Aldrich ...
-
bioRxiv - Neuroscience 2023Quote: ... and Plp1-TAT-DTR mice (for P42_new IHCs) were dissected out and first digested in a choline chloride solution containing 20 U/mL papain (Cat#: LK003178, Worthington) and 100 U/mL DNase I (Cat# ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Cell Biology 2023Quote: ... The tissue pieces from each sample were weighed and incubated with 50 U/mL collagenase type 3 (#CLS-3, CAT#LS004182, Worthington) in Hibernate-E medium (at ratio of 8μL/mg tissue ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004182, 1 gm, $215.00 Ask -
bioRxiv - Physiology 2023Quote: ... Hearts were then perfused for 5 - 10 min with 0.125 mM CaCl2 KRH buffer supplemented with 1mg/mL of type II collagenase (Worthington Biochemical). Ventricles were cut into 0.1 mM CaCl2 solution ...