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Citations for Worthington Biochemical :
51 - 91 of 91 citations for Tryptic Soy Agar TSA CRITERION Dehydrated Culture Media since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Cells were then DNAse treated with 4 ml dissagregation media (DMEM, 10% FBS, 100 μg/ml DNAse (Worthington)) for 20 min at 37 °C ...
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bioRxiv - Biophysics 2023Quote: ... The media was cut into small pieces and immersed in tubes containing both elastase (Elastase, Lyophilized ESL, Worthington) and collagenase (Collagenase ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was dissociated enzymatically in DMEM media containing collagenase type I (1.66 mg/mL, Cat# LS004194; Worthington) and neutral protease (1.04 mg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... Media was aspirated and cells were resuspended in DMEM/F12 supplemented with 100ug/mL DNase (Worthington Biochemical, LS002006). After incubating in DNase ...
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bioRxiv - Cell Biology 2021Quote: Single myofiber cultures were established from EDL muscle after digestion with collagenase II (Worthington Biochemical Corporation, Lakewood, NJ) and trituration as described (Hindi and Kumar ...
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bioRxiv - Neuroscience 2021Quote: ... Dissected brains were chilled on ice and minced in digestion media containing with 0.2% collagenase type 3 (LS004182, Worthington) and 3 U/mL dispase (LS02104 ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004182, 1 gm, $215.00 Ask -
bioRxiv - Cell Biology 2020Quote: ... and then they were incubated in 20 mL of DMEM media containing 0.2 % collagenase type II (Worthington Biochemical Corporation) and 2.5 U/mL dispase (Gibco ...
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bioRxiv - Immunology 2023Quote: ... Cells were then DNase treated with 4 ml disaggregation media (DMEM, 10% FBS, 100 μg/ml DNASE I (Worthington)) for 20 min at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: Differentiated human neural cultures (3 months old) were dissociated into single-cells suspension using Papain Dissociation System (Worthington #LK003150), passed through 70µm and 40µm cell strainers (Falcon #07-201-431 and #07-201-430) ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Microbiology 2020Quote: ... A saturated overnight LB culture was diluted 1:50 into 3mL of minimal medium with or without 0.2g/L cellulase (Worthington, purified exo- and endo-glucanases ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Ganglia were transferred to dissociation media (divalent cation-free HBSS with 2 mg/mL collagenase Type II (CLS2, Worthington, USA) for 60 min at 37 °C ...
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... The tissue was then titurated in a digestion media containing 10 U/ml elastase (#LS002280, Worthington Biochemical, Lakewood, NJ, USA) and incubated for 30 minutes at 37°C ...
Product cited:
Two times crystallized. Supplied as an aqueous suspension. This preparation must be diluted to...Cat# LS002280, 1 gm, $1065.00 Ask -
bioRxiv - Microbiology 2020Quote: ... Media was changed 18-24 hours post-transfection, with 2 mL of virus growth media (MEM, 1 mM HEPES, 1 μg/mL TPCK trypsin (Worthington Biochemical Corporation ...
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Nucleoprotein reassortment enhanced transmissibility of H3 1990.4.a clade influenza A virus in swinebioRxiv - Microbiology 2023Quote: ... the media was supplemented with 1 µg/ml of tosylsulfonyl phenylalanyl chloromethyl ketone (TPCK) treated-trypsin (Worthington Biochemicals, Lakewood, NJ). Wild-type viruses were generated with their respective NP genes (wtNC/19-pdmNP and wtMN/18-trigNP) ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse hindlimb muscles were dissected and incubated in digestion media (2.5 U/mL Dispase II; Thermo and 0.2% w/v Collagenase Type II; Worthington in DMEM) on a shaking incubator at 37°C for 1.5 hours ...
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bioRxiv - Cell Biology 2024Quote: ... the dissociation media was removed from the cortices and replaced with papain solution (5 mL 1x dissociation media, 5-7 grains of L-Cyteine, and 172 µL Worthington papain [crystalline suspension in 50mM sodium acetate ...
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bioRxiv - Cell Biology 2020Quote: Organoid cultures within 2-3 weeks of primary plating were dissociated with 1 U/ml neutral protease (Worthington, Cat LS02100) and 100 KU of DNase I in organoid media ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02100, 10 mg, $84.00 Ask -
bioRxiv - Genomics 2020Quote: ... samples were cut into small pieces in artificial cerebrospinal fluid before being added into pre-warmed papain dissociation media (Worthington #LK003150). The dissociation solution was incubated for 45 minutes at 37°C ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Cancer Biology 2021Quote: ... Fresh tumor tissue post resection was collected in the HiberbateTM media and dissociated with an enzyme cocktail including 32 mg of Collagenase IV (Worthington #LS0042019); 10 mg of Deoxyribonuclease I (Worthington #LS002007 ...
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bioRxiv - Immunology 2020Quote: ... and both fragments were separately digested in a petri-dish at 37°C 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (12 hours) ...
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bioRxiv - Immunology 2024Quote: ... these tissues were finely minced and then digested at 37℃ in digestion buffer: RPMI 1640 (UCSF Media Production) with 1 mg/mL collagenase IV (Worthington Biochemical) and 0.1 mg/mL DNase I (Sigma-Aldrich) ...
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bioRxiv - Genomics 2023Quote: ... Each sample was centrifuged at 1200 rpm x5 mins at 4°C and resuspended in 10 mL warmed digestive media (HBSS + 1% HEPES) with 1,000 U/mL collagenase type IV (Worthington, Cat: LS004188), and rotated for 30 min at 37 °C ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Cancer Biology 2024Quote: ... The remaining tissue fragments on the strainer were resuspended in DMEM/F-12 basal media and digested with 250 U/ml Collagenase Type II (Worthington, 4176) at 37°C water bath for 10 min ...
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bioRxiv - Synthetic Biology 2024Quote: ... and both fragments were separately digested in a petri dish at 37°C 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) for 12 hours ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated at 37°C in serum-free cell culture medium (IMDM) supplemented with 1 µg/ml L-1-tosylamide-2-phenylethyl chloromethyl ketone (TPCK) trypsin (Worthington-Biochemical). Cell supernatants were harvested at indicated time points post-infection ...
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bioRxiv - Neuroscience 2020Quote: Dissociated DRG cultures for calcium imaging were prepared from freshly excised DRGs according to our previously published protocol (see “Dissociated dorsal root ganglia cultures”, 11) with STEMzyme I (2mg/ml; Worthington, LS004106) replacing collagenase IV ...
Product cited:
A specialized combination of Animal Free Clostridium histolyticum collagenase (Code: CLSAFB) and...Cat# LS004106, 50 mg, $110.00 Ask -
bioRxiv - Genomics 2024Quote: ... transposition and library construction were performed as described in.36 100,000 cells from cell cultures with high viability (above 90%) were treated with DNAse (Worthington Cat# LS002007) at a concentration of 200U/ml in culture medium at 37°C for 30 minutes ...
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bioRxiv - Genomics 2022Quote: Primary cultures of neonatal rat ventricular cardiomyocytes (CM) were isolated as previously described using the neonatal cardiomyocyte isolation system (Worthington Biochemical Corporation) from 1-3 day old Fischer rat pups (Golan-Lagziel et al. ...
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Pax6 loss alters the morphological and electrophysiological development of mouse prethalamic neuronsbioRxiv - Developmental Biology 2021Quote: ... the prethalamus from the two halves of the neural tube of the same embryo were put together and chopped into smaller pieces for dissociated cell culture using the Papain Dissociation System (Worthington Biochemical Corp.) according to the manufacturer’s protocol (Figure 2C-E) ...
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bioRxiv - Cancer Biology 2023Quote: ... the cerebral cortices were minced with scalpels on an ice-cold culture dish and digested using a papain dissociation kit (Worthington Biochemical, LK003150), according to the manufacturer’s instructions ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Immunology 2024Quote: ... and then incubated in 5 mL digestion buffer (basal media, 10 µM Y-27632, 600 U/mL Collagenase IV (Worthington cat. No. LS004189), 0.1 mg/mL DNAse I (Sigma-Aldrich ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004189, 5 gm, $900.00 Ask -
bioRxiv - Cancer Biology 2022Quote: ... The obtained liver tissues were homogenized in 8 mL of RPMI1640 media containing 1.8 mg/ml Collagenase IV (WOR-CLS4-1, Worthington Biochemical Corporation, NJ, USA) and 112.3 µg/ml DNase I (11284932001 ...
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bioRxiv - Cancer Biology 2024Quote: ... minced in F12 medium and resuspended in 10 mL of digestion medium (minimum essential media containing 112 U/mL type I collagenase; Worthington, cat. #CLS-1), 1.2 U/mL dispase (Gibco ...
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bioRxiv - Genomics 2023Quote: Primary neuronal cultures were prepared from embryonic day (E18) mice by dissecting the cortices and placing them in a papain solution (Worthington Biochemical, Cat# LS003126) dissolved in a basic culture medium (Neurobasal medium (Gibco ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Immunology 2023Quote: ... Virus-infected cells were grown in MEM-BSA-TPCK media[20] that was prepared similar to the MEM media described above but supplemented with 1 µg/mL Tosyl phenylalanyl chloromethyl ketone (TPCK) trypsin (Worthington Chemical Corporation, Lakewood, NJ) and with Bovine Albumin Fraction V (7.5% solution ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and 24 h post-transfection the media was supplemented with 1 μg/mL of tosylsulfonyl phenylalanyl chloromethyl ketone (TPCK)-treated trypsin (Worthington Biochemicals, Lakewood, NJ, USA). The rgH9N1 viruses were propagated on MDCK cells and harvested 72 hours post-inoculations ...
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bioRxiv - Molecular Biology 2024Quote: ... Prior to beginning the ATAC-seq protocol drug-containing media was replaced with 200 units/mL DNase (LS002006, Worthington Biochemical Corp, Lakewood, NJ, USA) in 1.2 mM MgCl2 and 0.5 mM CaCl2 for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were centrifuged and then resuspended in a pre-warmed DNase solution (L-WRN media supplemented to a final concentration of 200U/ml DNase [Worthington], 2.5mM MgCl2, 500mM CaCl2) and incubated for 10min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: The ovarian cancer tissues were incubated in DMEM high glucose culture medium supplemented with 3 mg/ml collagenase D (Worthington-biochem, Lakewood, NJ, USA) at 37°C for 45 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were centrifuged and then resuspended in a pre-warmed DNase solution (L-WRN media supplemented to a final concentration of 200U/ml DNase [Worthington], 2.5 mM MgCl2, 500 μM CaCl2) and incubated for 5-7 minutes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were centrifuged and then resuspended in a pre-warmed DNase solution (L-WRN media supplemented to a final concentration of 200U/ml DNase [Worthington], 2.5 mM MgCl2, 500 mM CaCl2) and incubated for 10 min at 37°C ...