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Citations for Worthington Biochemical :
1 - 50 of 91 citations for Tryptic Soy Agar TSA CRITERION Dehydrated Culture Media since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Spn were grown on tryptic soy (TS; Becton Dickinson) agar plates supplemented with 100 μl of catalase (30,000 U/ml; Worthington Biomedical) and appropriate antibiotics (200 μg/ml streptomycin ...
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bioRxiv - Neuroscience 2023Quote: ... The L2-5 ganglia were dissected out and placed in cell culture media before being transferred to media containing neutral protease 3.125 mg/mL (Worthington, LS02104) and collagenase type 1 5 mg/mL (Worthington ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02104, 50 mg, $352.00 Ask -
bioRxiv - Cell Biology 2020Quote: ... followed by a tryptic digestion in gel (TPCK treated trypsin, Worthington Biochemical Corporation). The digests were separated with a reversed phase nano-spray column (NTCC-360/75-3-105 ...
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bioRxiv - Cell Biology 2022Quote: ... followed by a tryptic digestion in gel (TPCK treated trypsin, Worthington Biochemical Corporation). The digests were separated with a reversed phase nano-spray column (NTCC-360/75-3-105 ...
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bioRxiv - Microbiology 2020Quote: ... All four cell lines were infected with SARS-CoV-2/Australia/VIC01/2020 at a multiplicity of infection of 0.1 with infection inoculum composed of serum-free culture media and TPCK-treated trypsin (Worthington). The plates were incubated at 37 °C for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Cortex tissue was then incubated in digestion buffer (complete culture media with 6% (v/v) papain (Worthington Biochemical, Cat#LS003126) and 750U/µl DNase I (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... followed by 10X dilution into 50 mM ABC (pH 8.0) and tryptic digestion at 37°C for 20 hr with shaking using 1 μg of trypsin (Worthington Biochemical Corp., Lakewood, NJ) per 50 μg protein ...
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bioRxiv - Microbiology 2022Quote: ... Bacterial numbers were enumerated by plating serial dilutions on TSA plates supplemented with 100 µl of catalase (38,000 U/ml; Worthington Biochemical Corporation, NJ) and the desired antibiotic (250 µg/ml kanamycin ...
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bioRxiv - Microbiology 2022Quote: ... Cultures were incubated statically at 37°C with 5% CO2 followed by plating on TSA plates supplemented with 100 µl of catalase (38,000 U/ml; Worthington Biochemical Corporation, NJ) and the desired antibiotic (250 µg/ml kanamycin or 200 µg/ml streptomycin) ...
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bioRxiv - Microbiology 2022Quote: ... 18-24hrs post transfection, the media was replaced with viral growth media (MEM, 1 mM HEPES, 1 μg/mL TPCK trypsin (Worthington Biochemical Corporation ...
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bioRxiv - Microbiology 2020Quote: ... 1 hour after infection media was changed to serum-free media supplemented with 1µg/ml tosyl phenylalanyl chloromethyl ketone (TPCK)-treated trypsin (Worthington-Biochemical). Passage experiments were performed by infecting cells at an MOI of 0.01 ...
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bioRxiv - Developmental Biology 2021Quote: ... per mL of EHAA media and DNAse (Worthington). An equal volume of 0.1 M EDTA in Hank’s buffered saline solution without calcium or magnesium was added to the digested cells and incubated for 5 min at 37°C ...
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bioRxiv - Biophysics 2024Quote: ... pelleted and resuspended in L15 media (Worthington, NJ). Cells were then subjected to Percoll gradient for separation of cardiomyocytes from non-myocytes ...
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bioRxiv - Biophysics 2021Quote: ... this media is similar to the MEM media described above but supplemented with Tosyl phenylalanyl chloromethyl ketone (TPCK) trypsin (Worthington Chemical Corporation) and with Bovine Albumin Fraction V (7.5% solution ...
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bioRxiv - Immunology 2021Quote: ... tissue was finely minced and placed into digestion media (RPMI basal media, 300 U/mL collagenase I [Worthington Biochemical Corporation], 1% BCS), shaken for 30 min at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... neuronal cultures were dissociated with papain (Worthington biochemical Corporation) supplemented with Accutase (Sigma ...
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bioRxiv - Immunology 2020Quote: ... per ml of EHAA media with DNAse (Worthington, Lakewood, NJ) at 37 degrees ...
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bioRxiv - Immunology 2024Quote: ... per mL of EHAA media with DNAse 1(Worthington, Lakewood, NJ) at 37°C to isolate the stromal cell population for 1 hr at 37°C with pipetting every 15 min to physically agitate the digested tissues ...
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bioRxiv - Neuroscience 2023Quote: ... Cortices were dissociated in Neurobasal media containing papain (20U/mL, Worthington) and DNase I (100 μg/mL ...
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bioRxiv - Immunology 2023Quote: ... Digestion media was prepared with 0.8 mg/mL Collagenase IV (Worthington) and 0.05 mg/mL DNase I (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... Media was replaced with collagenase II (200 U/mL, Worthington, LS004177)/dispase II (3.75 U/mL ...
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bioRxiv - Genomics 2024Quote: ... cells were treated in culture medium with DNase (Worthington cat# LS002007) at a final concentration of 200 U/mL at 37 °C for 30 mins ...
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bioRxiv - Neuroscience 2023Quote: ... washed 5 minutes with Neurobasal media containing Ovomucoid (15 mg/mL, Worthington), and manually triturated in OptiMeM supplemented with D-Glucose (20mM) ...
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bioRxiv - Neuroscience 2023Quote: ... They were then dissociated in Neurobasal media containing papain (20U/mL, Worthington) and DNase I (100 μg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... Primary cultures of neurons were prepared using the Papain Dissociation System (Worthington) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... cells in culture were treated with 200 U/ml DNase (Worthington # LS002007) for 30 min at 37°C to remove free-floating DNA and any DNA from dead cells ...
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bioRxiv - Neuroscience 2021Quote: ... cultures were dissociated into single-cells suspension using Papain Dissociation System (Worthington #LK003150), passed through 70 µm cell strainer (Falcon #07-201-431) ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Developmental Biology 2020Quote: ... differentiated cultures were dissociated by incubating with 0.6mg/ml Papain (Worthington Biochemical, LS003118) in CMF-PBS (dPBS ThermoFisher ...
Product cited:
Supplied as a lyophilized powder prepared from a 2X crystalline suspension, Code: PAP. To...Cat# LS003118, 25 mg, $44.00 Ask -
bioRxiv - Cell Biology 2020Quote: ... Digestion was performed in RPMI media containing 20 U/mL papain (Worthington Biochemical LS003119) and 0.005% DNAase I ...
Product cited:
Supplied as a lyophilized powder prepared from a 2X crystalline suspension, Code: PAP. To...Cat# LS003119, 100 mg, $100.00 Ask -
bioRxiv - Immunology 2023Quote: ... and incubated with digestion buffer (RPMI media supplemented with (2mg/ml) collagenase IV (Worthington) and (187.5 μg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... Retinas were then incubated in digestion media containing papain (16.5U/mL; Cat# LK003176; Worthington) and DNase I (12,500U/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... aortic media tissue was digested with a solution of collagenase and elastase (Worthington Biochem) until a single-cell suspension was obtained ...
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bioRxiv - Genomics 2021Quote: ... and treated each T cell culture with 200U/mL of DNase (Worthington Cat # LS002007) for 30 mins at 37 degrees ...
Product cited:
Chromatographically purified. A lyophilized powder with glycine as a stabilizer.Cat# LS002007, 100 mg, $350.00 Ask -
bioRxiv - Neuroscience 2022Quote: ... 4 cultures were pooled and dissociated using the Papain Dissociation System (Worthington Biochemicals, LK003150) according to manufacturer’s instructions ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Cell Biology 2021Quote: ... digested in Click’s media containing 500 units/mL Collagenase II (Worthington, Columbus, OH, USA; LS004174) and Collagenase IV (Worthington ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004174, 100 mg, $42.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... and the pellet was resuspended in 5 mL dissociation media containing 165U of Papain (Worthington) and DNAse type I (40 µg/mL ...
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bioRxiv - Physiology 2024Quote: ... and digested with pre-warmed collagenase media (500 U/mL collagenase type 2 (LS004183, Worthington) and 3.75U/mL DNase I (10104159001 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary cultures of neurons were prepared from cerebral cortices using the Papain Dissociation System (Worthington) according to the manufacturer’s instructions and were plated and cultured as described above for 5xFAD mouse neurons ...
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bioRxiv - Molecular Biology 2022Quote: Crosslinked spheroplasts from 100 ml cultures were treated with 1600 U MNase (Worthington Biochemical Corporation) for 1-1.5 h at 37°C and mononucleosomal-sized DNA was prepared following gel electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: Day 85 cultures from 16 lines were detached enzymatically in parallel using papain (Worthington; LK003153) and were filtered through 40µm Flowmi Cell Strainers (Scienceware ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003153, 3 bx, $775.00 Ask -
bioRxiv - Molecular Biology 2022Quote: ... then digested with collagenase II (1100 U ml-1, Worthington in Hams F-10 media (Sigma)) for 90 min at 37°C with gentle rotation at 70 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated for 7 min with Neurobasal media containing Ovomucoïde (15 mg/mL, Worthington), and manually triturated 10 times with a 1000 ml pipette in OptiMeM supplemented with D-Glucose (20mM) ...
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bioRxiv - Cancer Biology 2024Quote: ... Lung tissue bits were resuspended in the wash media containing 1 mg/ml collagenase I (Worthington) and 1 mg/ml collagenase IV (Worthington ...
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bioRxiv - Immunology 2024Quote: ... lungs were perfused and inflated with digestion media containing Elastase (4.5 U/mL, Worthington, Catalog #: LS002280) and DNase (20 mg/mL ...
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bioRxiv - Pathology 2024Quote: ... followed by resuspension in media containing 1 mg/ml collagenase A (Worthington Biochemical, Lakewood, NJ, USA) and 0.2 mg/ml of DNAse I (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... and transferred to a culture dish containing deoxyribonuclease I (100 U/ml) (Worthington Biochemicals, Malvern, PA). The parenchymal lung tissue was gently teased from the bronchi ...
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bioRxiv - Neuroscience 2020Quote: ... The following modifications were made to enhance culture health: tissue pieces were digested with papain (Worthington) instead of trypsin-EDTA ...
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bioRxiv - Neuroscience 2021Quote: ... was added to halt the proteolysis and samples were centrifuged at 300xG for 10 minutes at 4°C and resuspended in a 1:1:10 solution of DNase:ovomucoid:Neurobasal media (Worthington:Worthington:Gibco). Cell suspensions were incubated with primary antibodies to CD11b and CD45 (table 1 ...
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bioRxiv - Immunology 2023Quote: ... Tissues were mechanically cut and digested in complete media containing Collagenase type 2 (265 U/mL; Worthington) and DNase (250 U/mL ...
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bioRxiv - Neuroscience 2021Quote: ... was added to halt the proteolysis and samples were centrifuged at 300xG for 10 minutes at 4°C and resuspended in a 1:1:10 solution of DNase:ovomucoid:Neurobasal media (Worthington:Worthington:Gibco). Cell suspensions were incubated with primary antibodies to CD11b and CD45 (table 1 ...