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Citations for Worthington Biochemical :
451 - 500 of 516 citations for D Fructose U 13C6 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Cortices were then digested for 30 minutes at 37°C using a digestion medium containing papain (25 U/ml, Worthington Biochemical), DNAse I (2000 U/ml ...
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bioRxiv - Neuroscience 2021Quote: ... Cortices were then digested for 30 minutes at 37°C using a digestion medium containing papain (25 U/ml, Worthington Biochemical), DNAse I (2000 U/ml ...
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bioRxiv - Neuroscience 2021Quote: ... The tissue was digested for 30 min using 2 mL of HEPES-buffered HBSS containing 20 U/mL of papain (Worthington Biochem.), 1 mM EDTA (PanReac AppliCham ...
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bioRxiv - Neuroscience 2023Quote: ... Hippocampi were isolated from P0 CD-1 mouse brain tissues and digested with 10 U/mL papain (Worthington Biochemical Corp., LK003176). Mouse hippocampal neurons were plated on following poly lysine-coated dishes for each experiment - glass bottom dishes (500,000 cells ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Cell Biology 2023Quote: ... the tissues were further transferred into 20 mL of pre-warmed digestion buffer (RPMI medium containing 10% FBS, 1% P/S, 15 mM HEPES, 25 U/mL of collagenase IV (Worthington LS004186), 100 U/ml of DNase I (Sigma D4513) ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Bioengineering 2023Quote: ... hACs were isolated using 10 ml of a 0.15% (w/v) type II collagenase (300 U mg−1; Worthington Biochemical Corporation) solution per g of tissue (37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... which included a first step of PDX tumor mechanical dissociation with sterile scalpels and then enzymatic dissociation by trypsin (10 mg/ml) and type II collagenase (275 U/mg, Worthington Biochemical). The tube was then placed in a warm 37°C water bath for 60 min ...
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bioRxiv - Molecular Biology 2024Quote: ... nuclei from 2 X 108 Hela or 293T cells were digested with 20 U of micrococcal nuclease (Worthington Biochemical Corporation, LS004797) for 45 minutes ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004797, 15 ku, $94.00 Ask -
bioRxiv - Genomics 2023Quote: ... Each sample was centrifuged at 1200 rpm x5 mins at 4°C and resuspended in 10 mL warmed digestive media (HBSS + 1% HEPES) with 1,000 U/mL collagenase type IV (Worthington, Cat: LS004188), and rotated for 30 min at 37 °C ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Cortices were isolated from P0 CD-1 mouse brain tissues and digested with 10 U/mL papain (Worthington Biochemical Corp., LK003176). Mouse cortical neurons were plated on following poly lysine-coated glass bottom dishes (500,000 cells) ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Homogenate was digested for 15 minutes in enzymatic solution (0.2 µm filter sterilised 4.5 U/mL papain [Worthington Biochemicals], 1× DNAse [Worthington Biochemicals], in PBS) at 37 °C ...
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A balance between pro-inflammatory and pro-reparative macrophages is observed in regenerative D-MAPSbioRxiv - Bioengineering 2023Quote: ... the implants were enzymatically digested with the digestion solution (200 U/ml Collagenase IV and 125U/ml DNase I, both from Worthington Biochemical) in RPMI media (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... Excised hearts were cannulated to the Langendorff retrograde perfusion system through the aorta and perfused with Base solution (Tyrode’s solution with 10 mM taurine and 12 mM 2,3-butanedione monoxime) and then digested with prewarmed Digestion buffer (Base solution with 180 U/mL Collagenase Type II (Worthington, LS004177) and 25 μM CaCl2 ...
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bioRxiv - Immunology 2024Quote: ... The ganglia were placed into a digestion buffer composed of 1 mg/ml (325 U/ml) collagenase type 4 (Worthington LS004189), 2 mg/ml (1.8 U/ml ...
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bioRxiv - Neuroscience 2024Quote: ... 0.2 mg/mL L-cystein (MP, #101444) 0.2 mg/mL BSA (Multicell, #800-095-EG) and 20 U/mL DNAse I (Worthington Biochemical, #LS006333) in PBS 1X and centrifuged at 2000g for 2 min ...
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bioRxiv - Cancer Biology 2024Quote: ... The remaining tissue fragments on the strainer were resuspended in DMEM/F-12 basal media and digested with 250 U/ml Collagenase Type II (Worthington, 4176) at 37°C water bath for 10 min ...
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bioRxiv - Bioengineering 2024Quote: Cardiomyocytes were dissociated by Langendorff heart perfusion of a 37°C digestion perfusion solution containing 300 U/mL collagenase (Worthington, LS004176). Collagenase digested tissue was minced and dissociated cardiomyocytes filtered through a 100 µm mesh filter (Falcon ...
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bioRxiv - Cell Biology 2024Quote: ... and 10 µM ROCK inhibitor Y-27632: MedChem Express HY-10583) containing Collagenase III (300 U/mL: Worthington Biochemical Corporation LS004182) and DNase I (200 U/mL ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue from 2-3 hypothalamiwas pooled into 1-1.5 mL of HABG containing papain (20 u/mL, Worthington Biochemical, Lakewood, NJ, US). Tissue was broken into smaller pieces by pipetting 2-3 times with a P1000 pipet followed by a fire-polished glass pipette with a wide opening ...
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bioRxiv - Neuroscience 2021Quote: ... The tissue was digested for 30 min using 2 mL of HEPES-buffered HBSS containing 20 U/ml of papain (Worthington Biochem., USA), 1 mM EDTA (PanReac AppliChem ...
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Abl kinase deficiency promotes AKT pathway activation and prostate cancer progression and metastasisbioRxiv - Cancer Biology 2020Quote: ... GS689.Li cells from primary tumors were recovered by mincing with a sterile razor blade and digesting with 200 U/ml collagenase II (Worthington Biochemical Corporation) for 15 min at 37°C ...
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bioRxiv - Bioengineering 2021Quote: The elastin degraded model was produced by incubating native porcine carotid arteries in 10 U/ml purified elastase (ESFF, Worthington Biochemical Corporation) with 0.35 mg/ml trypsin inhibitor (10109886001 ...
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bioRxiv - Bioengineering 2021Quote: ... The tissue was digested for 30 min using 2 mL of HEPES-buffered HBSS containing 20 U/ml of papain (Worthington Biochem., USA), 1 mM EDTA (PanReac AppliChem ...
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bioRxiv - Genomics 2021Quote: ... Lung tumor sample were cut into small pieces with a razor blade and enzymatically digested (100 U/mL Collagenase IV, Sigma-Aldrich, C5138-1G; 50 µg/mL DNase 1, Worthington, LS002138) for 35 minutes being rotated at 37°C in HEPES buffered RPMI 1640 containing 0.5% FBS ...
Product cited:
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bioRxiv - Neuroscience 2020Quote: ... Acute bilateral fastigial nuclei were quickly excised with a sharp knife from 2 or 3 cerebellar slices and were then enzymatically digested with cysteine (2 mM)-supplemented papain (40 U/ml, Worthington, Lakewood, NJ) and chondroitinase-ABC (1 U/ml ...
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bioRxiv - Immunology 2021Quote: ... tissue was finely minced and placed into digestion media (RPMI basal media, 300 U/mL collagenase I [Worthington Biochemical Corporation], 1% BCS), shaken for 30 min at 37°C ...
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bioRxiv - Bioengineering 2021Quote: The collagen degraded model was produced by incubating native porcine carotid arteries in 1000 U/ml purified collagenase (CLSPA, Worthington Biochemical Corporation) in magnesium chloride and calcium chloride supplemented PBS (D8662 ...
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bioRxiv - Neuroscience 2023Quote: ... the medial PFC of ECFP-expressing P1 mice was dissected and dissociated with papain (20-25 U/mL; Worthington Biochemical, Lakewood, NJ) at 37°C for 30 minutes ...
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Acinar-to-ductal metaplasia in the pancreas requires a glycolytic switch and functional mitochondriabioRxiv - Cancer Biology 2022Quote: ... specimens were cut into pieces and digested twice with 10 ml/g tissue of 50 U/ml collagenase (CLSPA Worthington Biochemical Corporation) in SOL1 for 10 min ...
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bioRxiv - Cell Biology 2023Quote: Tissue samples were dissected into small pieces and digested with 1-2 mg/ml (275 U/ml) collagenase type II (Worthington, Columbus, USA) in 30 ml Dulbecco’s minimal essential medium (DMEM ...
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bioRxiv - Physiology 2024Quote: ... CD1 wild type) were harvested and enzymatically digested for 45 min at 37 °C with collagenase type II (520 U/ml, Worthington, NJ US) (20) ...
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bioRxiv - Neuroscience 2024Quote: ... 4–5 randomly selected organoids were pooled to obtain single cell suspension and then incubated in 30 U/mL papain enzyme solution (Worthington Biochemical, LS003126) and 0.4% DNase (12,500 U/mL ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Retinas were detached from the retinal pigmented epithelium and incubated for 15 min at 37°C in a solution containing papain (10 U/ml, Worthington; Lakewood, NJ) and L-Cysteine hydrochloride (∼1 mg/mL ...
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bioRxiv - Microbiology 2022Quote: ... Bacterial numbers were enumerated by plating serial dilutions on TSA plates supplemented with 100 µl of catalase (38,000 U/ml; Worthington Biochemical Corporation, NJ) and the desired antibiotic (250 µg/ml kanamycin ...
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bioRxiv - Microbiology 2022Quote: ... The barcoded Spn transformants were selected on TS plates supplemented with 100 µl of catalase (38,000 U/ml; Worthington Biochemical Corporation, NJ) and 200 µg/ml spectinomycin ...
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bioRxiv - Microbiology 2022Quote: ... Cultures were incubated statically at 37°C with 5% CO2 followed by plating on TSA plates supplemented with 100 µl of catalase (38,000 U/ml; Worthington Biochemical Corporation, NJ) and the desired antibiotic (250 µg/ml kanamycin or 200 µg/ml streptomycin) ...
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bioRxiv - Cancer Biology 2024Quote: Mechanical tissue dissociation was performed using scissors and enzymatic dissociation was performed using 1000 U/mL Type IV collagenase (Worthington, Cat: LS004188) at 37 °C for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Dissected frontal cortex (cortex rostral to the optic chiasm) was minced and immersed in an enzyme incubation solution (1 mL) containing 200 U papain (Worthington Biochemical, #LS003126), 0.5 mM EDTA (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... the ganglia were transferred to freshly prepared pre-warmed dissociation medium composed of 15 U/mL Papain suspension (Worthington Biochemical; Catalog#: LS003126), 0.3 mg/mL L- cysteine (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... Four or five randomly selected organoids or assembloids were pooled to obtain single cell suspension and then incubated in 30 U/mL papain enzyme solution (Worthington Biochemical, LS003126) and 0.4% DNase (12,500 U/mL ...
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bioRxiv - Bioengineering 2024Quote: ... The DRGs from 4 pups were digested for 30 min at 37°C using 13.33 U/ml papain (Worthington, cat. No. LS003126). The DRGs were then centrifuged for 1 min at 500g ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: Mouse lung tissue were cut into 2-mm pieces and digested with collagenase type D (2 mg/ml; Worthington) and deoxyribonuclease (DNase ...
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bioRxiv - Immunology 2024Quote: Single cell suspensions from Mtb-infected lungs were generated by digestion with 10mg/mL Type IV collagenase D (Worthington) and 30 μg/mL DNAseI (Roche ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: ... fresh lung tissue was minced into 0.1 cm pieces in petri dishes and treated with 2 mg/mL of collagenase D (Worthington) for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... The brains were rinsed in cold RNase-free PBS and transferred to freshly made dissociation buffer (300 μL of 100 U/mL heat-activated papain (Worthington Biochemical Corporation #LK003178) added with 6 μL of 2.5 mg/mL liberase™ (Sigma-Aldrich #5401119001)) ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... The remaining frozen tissue was weighed and briefly sliced on dry ice and then incubated in 75 U/ml collagenase type 3 (Worthington #CLS-3, S8P18814) in Hibernate-E solution (human tissue for 20 min ...
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bioRxiv - Neuroscience 2021Quote: ... The cerebellum was microdissected from either P21 or P42 animals and chopped into small pieces before incubation with papain digestion buffer (1x HBSS medium containing 40 U/ml Papain (Worthington Biochemical cat # LS003126), DNase I 100 units/ml (Worthington Biochemical cat # LK003172 ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Immunology 2023Quote: ... The tissue was transferred to 50 mL conical tubes with 5 mL of digestion solution pre-warmed to 37C and composed of 150 U/ml Collagenase type III (Worthington Biochemical, Freehold, NJ) and 50 µg/ml DNase I ...
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bioRxiv - Immunology 2024Quote: ... The tissue was transferred to 50 mL conical tubes with 5 mL of digestion solution pre-warmed to 37C and composed of 150 U/ml Collagenase type III (Worthington Biochemical, Freehold, NJ) and 50 µg/ml DNase I ...
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bioRxiv - Bioengineering 2024Quote: ... Tissue was minced until 1mm pieces remained, then lysed in equilibrated (37°C, 5% CO2) enzyme (20 U/mL Papain (Worthington Biochemical Corp; LK003176) in 5mL of EBSS (Sigma ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask