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Citations for Worthington Biochemical :
401 - 450 of 516 citations for D Fructose U 13C6 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and digested for 30 min using 2mL of HEPES-buffered HBSS containing 20 U/ml papain (Worthington Biochem., USA), 1mM EDTA (PanReac AppliChem ...
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bioRxiv - Cancer Biology 2021Quote: ... tissue sections were pre-treated with 50 μg/mL collagenase D (Worthington Biochemical Corp., Colorado, USA) in RPMI 1640 for 5 min ...
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bioRxiv - Cancer Biology 2021Quote: ... 100-200 mg of tumor bearing lung tissue was then thoroughly minced with Noyes scissors, prior to incubation with digestion buffer (HBSS supplemented with 5% HI FBS, 125 U/mL collagenase IV (Worthington) and DNase (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... tumors were mechanically dissociated and subjected to enzymatic digestion for 20 min with 200 U ml−1 collagenase IV (Worthington), 5 mM CaCl2 ...
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bioRxiv - Cell Biology 2020Quote: ... as well as on day 1 and 2 after RFA were dissociated using 20 U/ml papain43 (Worthington Biochemical, USA), filtered through a 70 μm filter and resuspended in 3% BSA in PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... and dissected kidneys were minced and incubated in digestion solution for 15 min at 37°C (collagenase II 300 U/ml (Worthington), pronase E 1 mg/ml (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... permeabilized cells were incubated with DNAse I recombinase (3-3000 U/ml in 50 mM Tris-HCL, pH 7.5, 1/ mg/ml BSA: Worthington, LK003170) prior to incubation with TUNEL mixture.
Product cited:
A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered...Cat# LK003170, 1 vi, $28.00 Ask -
bioRxiv - Bioengineering 2022Quote: ... The distal lung tissue was separated from the mainstem bronchi and transferred to a Petri dish containing DMEM with 1% HEPES and 100 U/mL DNase I (Worthington). The tissue was then dissociated with a blunt bone marrow aspiration needle ...
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bioRxiv - Microbiology 2020Quote: ... Spn were grown on tryptic soy (TS; Becton Dickinson) agar plates supplemented with 100 μl of catalase (30,000 U/ml; Worthington Biomedical) and appropriate antibiotics (200 μg/ml streptomycin ...
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bioRxiv - Molecular Biology 2020Quote: ... The remaining cut tissue sections were weighed and incubated with 50 U/mL collagenase type 3 (#CLS-3, CAT#LS004182, Worthington) digestion buffer (at ratio of 8μL / mg tissue ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004182, 1 gm, $215.00 Ask -
bioRxiv - Bioengineering 2022Quote: ... cells showed spontaneous beating and were dissociated on day 15 by incubation with 280 U/ml Collagenase (LS004174, Worthington, USA) and 40 U/ml DNase (LS006331 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004174, 100 mg, $42.00 Ask -
Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... The tissue was then titurated in a digestion media containing 10 U/ml elastase (#LS002280, Worthington Biochemical, Lakewood, NJ, USA) and incubated for 30 minutes at 37°C ...
Product cited:
Two times crystallized. Supplied as an aqueous suspension. This preparation must be diluted to...Cat# LS002280, 1 gm, $1065.00 Ask -
bioRxiv - Developmental Biology 2020Quote: ... The hearts from all embryos were then sorted into two tubes based on fluoresce signal and digested with 300μl 500 U/ml collagenase IV (Worthington #LS004186), 1.2 U/ml dispase (Worthington #LS02100) ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Cell Biology 2020Quote: Organoid cultures within 2-3 weeks of primary plating were dissociated with 1 U/ml neutral protease (Worthington, Cat LS02100) and 100 KU of DNase I in organoid media ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02100, 10 mg, $84.00 Ask -
bioRxiv - Developmental Biology 2020Quote: ... Ventricles of three hearts were micro-dissected and pooled into a 600 μl mix consisting of 1.2 U/ml dispase (Worthington #LS02100), 500 U/ml collagenase IV (Worthington #LS004186) ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02100, 10 mg, $84.00 Ask -
bioRxiv - Immunology 2020Quote: ... Lungs were instilled with either 1 mL of 10% formalin for histology studies or 1 mL of digestion buffer (90%DMEM 10% PBS+Calcium with 180 U/mL Type 2 Collagenase (Worthington) and 15 μg/mL DNase (Sigma ...
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bioRxiv - Physiology 2020Quote: ... muscles were surgically removed and enzymatically dissociated at 37 °C under mild agitation for ~1 h using 1,000 U/mL of collagenase type IV (Worthington Biochemical). Mechanical dissociation was completed using mild trituration in buffer with no collagenase ...
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bioRxiv - Cancer Biology 2021Quote: ... Liver was then digested by a pre-warmed digestion buffer consisting of HBSS and 5000 U collagenase I (Worthington Biochemical). The liver was then excised and cut on ice in GM and passed through a cell strainer ...
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bioRxiv - Neuroscience 2023Quote: ... Cortical tissues were isolated from P0 mouse brains and digested with 10 U/mL papain (Worthington Biochemical Corp. Lakewood, NJ). Mouse cortical neurons were plated on following poly lysine-coated glass bottom dishes (500,000 cells ...
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bioRxiv - Immunology 2022Quote: ... Aortae were dissected and digested in a solution containing of 1200 U/ml collagenase II (Worthington Biochemicals, Lakewood, NJ, USA), 60 U/ml DNase (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... and Plp1-TAT-DTR mice (for P42_new IHCs) were dissected out and first digested in a choline chloride solution containing 20 U/mL papain (Cat#: LK003178, Worthington) and 100 U/mL DNase I (Cat# ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Cell Biology 2023Quote: ... The tissue pieces from each sample were weighed and incubated with 50 U/mL collagenase type 3 (#CLS-3, CAT#LS004182, Worthington) in Hibernate-E medium (at ratio of 8μL/mg tissue ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004182, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2023Quote: Cerebral cortices from P4-6 CD1 mouse pups were dissected in ice-cold HBSS without Mg2+ and Ca2+ and triturated with 2 ml of digestion buffer containing 20 U/ml papain (Worthington) and 100 U/ml DNase I (Worthington ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Digestion was performed by applying 7 mL 1× HBSS containing 600 U/mL Collagenase II (Worthington Biochemical, Lakewood, NJ, USA) and 60 U/mL DNase I (AppliChem ...
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bioRxiv - Neuroscience 2022Quote: ... Retinas were washed 3 times for 3 min in L-15 solution and then placed in L-15 solution containing 7 U ml-1 papain (cat. no. 3126, Worthington Biochemical Corporation ...
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bioRxiv - Cell Biology 2023Quote: ... The minced muscles were centrifuged at 15,000 x g for 1 minute and transferred to 10 ml of digestion buffer (DMEM containing 2.5% HEPES, 1% penicillin/streptomycin, and 400 U/ml collagenase II (Worthington Biochemical)) and placed on an orbital shaker at 100 rpm for 1 hour at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Aorta samples were cut into small pieces and then transferred into an enzyme cocktail containing 450 U/mL collagenase type I (Worthington), 250 U/mL collagenase type XI (MilliporeSigma) ...
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bioRxiv - Genetics 2024Quote: Testicular cell suspension was prepared by incubation of seminiferous tubules in 200 U/ml Collagenase type I (Worthington Biochemical LS004196), 5 μg/ml DNAse I (Roche 10104159001) ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Immunology 2023Quote: ... The right lobes of the lungs were collected in 5 mL of digestion buffer (90% DMEM and 10% PBS and calcium with type 2 collagenase (180 U/ml) (Worthington) and DNase (15μg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... the hippocampi were washed twice with cold HBSS and individually incubated at 37°C for 20 min in Papain dissociation solution (45 U of papain (Worthington), 0.01% deoxyribonuclease (DNase) ...
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bioRxiv - Cell Biology 2023Quote: ... and individually incubated at 37°C for 15 to 20 min in a sterile solution of 45 U papain (Worthington), 0.01% DNase (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... and individually incubated at 37 °C for 15 to 20 min in a sterile solution of 45 U papain (Worthington), 0.01% DNase (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse hindlimb muscles were dissected and incubated in digestion media (2.5 U/mL Dispase II; Thermo and 0.2% w/v Collagenase Type II; Worthington in DMEM) on a shaking incubator at 37°C for 1.5 hours ...
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bioRxiv - Bioengineering 2022Quote: ... In vitro degradation of lignin composites was determined by submerging lignin composites in the solution of collagenase type II (0.5 U/mL, cat# CLS-2, Worthington Biochemical) with 1 mM CaCl2 in serum-free culture medium ...
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bioRxiv - Genetics 2022Quote: ... cortices and hippocampi acquired from 12 male mice from 3 separate litters were combined and dissociated using activated 20 U/mL Papain/DNase (Worthington) diluted in Hibernate A (Gibco ...
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bioRxiv - Biochemistry 2024Quote: ... The remaining cut tissue sections were weighed and incubated with 50 U/mL collagenase type 3 digestion buffer (#CLS-3, CAT#LS004180, Worthington) at ratio of 8 μL /mg tissue in a shaking water bath (25°C ...
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bioRxiv - Neuroscience 2024Quote: ... The neocortices were isolated from pups on day 0-1 in cold Earle’s Balanced Salt Solution (EBSS) and incubated for 15 min at 37°C in EBSS containing 10 U/ml papain (Worthington), followed by gentle dissociation in PBS containing 1.5 mg/ml BSA ...
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bioRxiv - Immunology 2024Quote: ... THP-1-monocyte-derived macrophages of 50,000 cells cultured in a medium were pretreated with 200 U/ml Deoxyribonuclease I (Worthington, LS002004) at 37 °C for 30 min to remove free-floating DNA and digest DNA from dead cells ...
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bioRxiv - Immunology 2024Quote: ... Livers were digested at 37°C for approximately 1 h in DMEM medium supplemented with 500 U/ml Collagenase II (Worthington). Erythrocytes were excluded using red blood cell lysis buffer (BioLegend ...
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bioRxiv - Neuroscience 2024Quote: ... The pellet was then digested at 37 °C for 15 min using 4.5 U/mL lyophilised papain (Worthington Biochemical, #LS003120), 5 mg/mL D-glucose (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... samples were pre-cut with small scissors and diluted and digested in 2:3 stock solution of CollagenaseI (10 U/ml, Worthington), Collagenase IV (400 U/ml ...
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bioRxiv - Neuroscience 2024Quote: ... Hippocampi were isolated from P0 C57Bl6J mouse brain tissues and digested with 10 U/mL papain (Worthington Biochemical Corp., LK003176). Mouse hippocampal neurons were plated on following poly lysine-coated dishes for glass bottom dishes (500,000 cells ...
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bioRxiv - Immunology 2024Quote: ... and inguinal skin draining lymph nodes from adult mice were enzymatically digested in 1mL of RPMI with 200 U/mL Collagenase IV (Worthington) and 0.2 mg/mL DNaseI (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... The RNA was then digested for 2 hr at 37 °C in 1/10 volume of 1 M ammonium bicarbonate with snake venom phosphodiesterase (1.2×10−4 U/μg RNA) and bacterial alkaline phosphatase (0.1U/ μg RNA) (Worthington Biochemical corporation). Analysis was performed on a TSQ Quantiva Triple Quadrupole (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... minced pieces (1-2 mm3) of isolated cerebral cortices were enzymatically digested in Hank’s buffered saline solution containing papain (20 U/ml, Worthington Biochemical), L-cysteine (1 mM ...
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bioRxiv - Bioengineering 2024Quote: ... The minced tissues were incubated in digest solution (2.5 U/ml Dispase II, MilliporeSigma and 0.2 w/v % Collagenase Type II, Worthington in DMEM) at 37°C for 90 minutes ...
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bioRxiv - Cell Biology 2020Quote: Freshly explanted NHP left ventricular myocardium was minced into small pieces and incubated with 550 U/ml collagenase II (Worthington, USA) at 37°C for 15 minutes in a series of 6 digestions ...
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bioRxiv - Neuroscience 2020Quote: ... transfer either only the cerebellum or the cerebellum including pones to a 15 mL tube containing 20 U/mL papain (Worthington, #LK003178) solved in preparation solution and warmed up to 36 °C ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... Hippocampi were dissected in ice-cold Hank’s balanced salts solution (HBSS) and kept on ice until digestion with 0.2 μm-filtered 10 U/mL papain (Worthington Biochemical Corporation) in HBSS in a 37°C water bath for 20 min ...
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bioRxiv - Neuroscience 2021Quote: ... SNc regions from the slices were dissected out with a scalpel blade and digested with oxygenated high glucose HEPES-buffered saline containing papain (5–8 U/ml, Worthington, USA) for 20–30 min at 37°C ...