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Citations for Qiagen :
2701 - 2750 of 6247 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... and real time quantitative PCR was carried out using SYBR Green Real Time PCR master mix (Qiagen). Normalization of target genes were done against TATA-box-binding protein (TBP ...
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bioRxiv - Cell Biology 2021Quote: ... PCR products for transformation were purified and concentrated with a commercial PCR purification kit (Qiagen, Maryland, USA). The deletion constructs (3μg ...
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bioRxiv - Molecular Biology 2021Quote: ... The fragments were amplified by PCR and purified using Qiagen MinElute PCR Purification Kit (Qiagen, Maryland, USA). The final library quality was controlled using the Agilent Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Purification of PCR product amplicons was performed with the QIAquick PCR Purification Kit (Cat. No. 28104, Qiagen). Quantification of the PCR amplified library was performed with the InvitrogenTM Quant-iTTM PicoGreenTM dsDNA Assay Kit (Cat ...
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bioRxiv - Genetics 2020Quote: ... cDNA was amplified with 12 cycles of PCR and purified using the QIAquick PCR Purification Kit (Qiagen). The libraries were size-selected on a 6% polyacrylamide gel ...
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bioRxiv - Plant Biology 2022Quote: qRT-PCR analyses were done according to Rotor-Gene SYBR Green PCR Kit instructions (QIAGEN, Hilden, Germany) and were run in a final volume of 15 μl for each tube containing 7.5 μl of Rotor-Gene SYBR Green PCR Master Mix ...
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bioRxiv - Plant Biology 2023Quote: ... Real-time quantitative PCR (qPCR) assays were performed in duplicate using RotorGene SYBR green PCR kit (Qiagen) on Rotor-Gene Q ...
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bioRxiv - Plant Biology 2023Quote: ... All PCR reactions were performed in 50µl volume containing: 25µl of Taq PCR Master Mix (QIAGEN, Germany), 2.5µl of BSA (0.2 μg/μL) ...
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bioRxiv - Microbiology 2022Quote: ... Ligation of 1 µl 5′RACE PCR products were performed with the Qiagen PCR cloning kit (Qiagen) according to the manufacters protocol ...
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bioRxiv - Genomics 2023Quote: The PCR was performed with the Multiplex PCR Kit (Type-It Microsatellite master mix; Qiagen, Hilden, Germany). The SSR primer mix consisted of eight primers (see Table 2 and Table 3) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR trials were run on the DNA extracts using the Qiagen Multiplex PCR kit (Qiagen, Hilden, Germany), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The PCR products were purified and concentrated with the QIAquick PCR Purification kit (Qiagen, cat. no. 28106). Homology arm and ultramer sequences are listed in Table S8.
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bioRxiv - Biophysics 2022Quote: ... The PCRs of each sample were then pooled and purified using the MinElute PCR Purification Kit (QIAGEN) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... PCRs for DH31 and DH31R alleles were conducted using the Taq PCR Master Mix Kit (Qiagen, # 201445) and all primers were synthesized by Microsynth (Balgach ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR products were then pooled in each group and purified using QIAquick PCR Purification Kit (Qiagen). Final NGS libraries were generated using 2ng of the purified 1st PCR product using the dual-indexing Illumina-compatible DNA HT Dual Index kit (Takara #R4000660,R400661) ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was amplified by polymerase chain reaction (PCR) with QuantiTect Sybr Green PCR Kit (Qiagen, Germantown, MD) on an Applied Biosystems QuantStudio 7 Flex real-time quantitative PCR instrument ...
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bioRxiv - Neuroscience 2021Quote: ... To each of those mixtures 150 μl of ice-cold MeOH was added and subsequently homogenized using a TissueLyser II (Qiagen). To support protein precipitation ...
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bioRxiv - Plant Biology 2021Quote: ... Fluorescence positive cells were collected in a round-bottom polystylene tube that contains 150 - 200 μl RLT buffer with beta-mercaptoethanol (10 μl / 1 ml RLT buffer, RNeasy micro kit protocol, Qiagen). Cell sorting was performed for about 15 min and collected cells were immediately frozen on dry ice and kept in −80 °C for up to one week ...
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bioRxiv - Plant Biology 2019Quote: Total RNA was extracted from 150 mg of leaf tissue using RNeasy plant mini RNA kit (50) (Qiagen, Valencia, CA), and RNA samples were further treated with DNase (Promega ...
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bioRxiv - Genetics 2021Quote: ... and 99F8 (150 ng pU6-3-99F8-gRNA (DGRC Cat# 1549) and 150 ng pBS-99F8-5’HR-attP>>Act5C::GFP<
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bioRxiv - Molecular Biology 2019Quote: ... DNA was separated on a 2% agarose gel and bands between 120-150 bp were excised and purified (QIAquick Gel Extraction Kit, Qiagen). Chromatin accessibility was assayed using quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Genomics 2021Quote: ... gel was viewed and cut between 150-500bp and DNA was extracted from gel pieces using QIAquick Gel Extraction Kit (QIAGEN). Libraries were normalized using the KAPA Library Quantification Kit (Illumina) ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR products with sizes from 150 bp to 300 bp were extracted from 2% agarose gel using MinElute Gel Extraction Kit (Qiagen).
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bioRxiv - Biochemistry 2023Quote: ... SUMO protease was added at a final concentration 1/10 and the mixture was dialyzed overnight at 4 °C against the buffer DB6 (50 mM Tris-HCl pH 8, 150 mM NaCl, 10 mM imidazole) and applied on a NiNTA column (Qiagen) equilibrated in the DB6 buffer ...
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bioRxiv - Microbiology 2023Quote: ... Then 4 μl RNase A (100 mg/ml) were added to the tubes followed by 150 μl of buffer AL (lysis buffer, Qiagen). After mixing by vortexing ...
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bioRxiv - Microbiology 2023Quote: ... 150 mg of soil were weighed to carry out the DNA extraction using the DNeasy PowerSoil kit (Qiagen, Toronto, ON) following the manufacturer’s protocol with the following modification ...
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bioRxiv - Genomics 2023Quote: ... Run time was set to 10 minutes and DNA between 150 and 500 bp was excised from the gel and purified using the QIAquick Gel Extraction Kit (Cat. #28706X4, Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 100 to 150 mg of powder was used to extract total RNA using the RNeasy Plant Mini Kit from QIAGEN. On-column DNase treatment was performed using the RNase-free DNase kit from Qiagen ...
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bioRxiv - Neuroscience 2023Quote: Hybridoma cells were lysed by resuspending the cell pellet in 150 µL of RNeasy Lysis Buffer (RLT) (Qiagen Catalog# 1015750) and transferred to the corresponding wells of a 96 well U bottom master block plate (Greiner Catalog# 780215) ...
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bioRxiv - Molecular Biology 2024Quote: ... Parasite genomic DNA was extracted from 50 µL of infected blood mixed with 150 µL PBS using the DNeasy Blood and Tissue kit (Qiagen). For pooled transfections ...
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bioRxiv - Cell Biology 2021Quote: ... The QIAGEN RNase-Free DNase Set was used for on-column DNA digestion according to the manufacturer’s protocol (Qiagen, Hilden, Germany, cat # 79256). Total RNA samples were sent to BGI Group ...
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bioRxiv - Genetics 2022Quote: Total RNAs were extracted from cells using the RNeasy extraction kit and treated with RNase free DNAse set during 20 min (Qiagen, Valencia, CA, USA) or from blood using PaxGene blood RNA kit extraction (Preanalytix ...
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bioRxiv - Neuroscience 2020Quote: ... RNA was then purified using a nucleic acid binding column with on-column DNase treatment (RNase-free DNase set, QIAGEN, Germantown, MD, USA). RNA was then eluted from the column in elution buffer.
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bioRxiv - Cancer Biology 2023Quote: ... were combined in sets of three curls per tube and extracted with the AllPrep® DNA/RNA FFPE Extraction Kit (PN 80234, Qiagen, Germantown, MD). Isolated nucleic acids were quantified by Qubit4™ (Qubit dsDNA HS kit ...
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bioRxiv - Biophysics 2020Quote: ... reaction mix (for PCR program details see Supplementary Table 1) and purified with QIAquick PCR Purification Kit (QIAGEN). The Fps1-mGFP strain was created by transforming the BY4741 background strain with 100 µL of the purified mGFP-HIS3 fragment with standard LiAc protocol to allow for homologous recombination 34 ...
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bioRxiv - Genetics 2021Quote: ... The PCR mix (final volume of 15.75 µL) consisted of: 7.5 µL Type-it Multiplex PCR Master Mix (QIAGEN), 3 µL Q solution (5X) ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmids and PCR products were purified with QIAprep plasmid Miniprep kit and QiaQuick PCR purification kit respectively (Qiagen). Plasmid Sanger sequencing was performed by Eurofins Genomics ...
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bioRxiv - Cell Biology 2020Quote: ... PCR was performed using TaKaRa Ex Taq® DNA Polymerase and the PCR products were purified by Qiagen PCR purification kit ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time qRT-PCRs were run in a Rotor-Gene Q real-time PCR cycler (Qiagen, Hilden, Germany) using the following program ...
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bioRxiv - Cancer Biology 2020Quote: ... gp130 expression was assessed by quantitative PCR on re-amplified and purified (Qiagen PCR Purification Kit, Qiagen, Germany) WTA products of single cells as previously described 74 ...
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bioRxiv - Cancer Biology 2020Quote: ... gp130 expression was assessed by quantitative PCR on re-amplified and purified (Qiagen PCR Purification Kit, Qiagen, Germany) WTA products of single cells as previously described 74 ...
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bioRxiv - Cancer Biology 2020Quote: ... Expression of miRNA was evaluated by q-PCR using a miScript SYBR@Green PCR kit (Cat # 218073, Qiagen).Screening of miRNA expression for 84 different miRNAs was performed on Exo-RNA isolated of CM cell lines and serum exosomes ...
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bioRxiv - Cell Biology 2020Quote: ... All PCR products were purified using either the QIAquick® PCR Purification Kit (Art. No. 28106, QIAGEN®) or the DNA Clean & Concentrator-5 Kit (Art ...
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Deconstructing the role of iron and heme in the structure of healthy human gut microbial communitiesbioRxiv - Microbiology 2022Quote: ... PCR products were individually cleaned up and quantified using the UltraClean 96 PCR Cleanup Kit (Qiagen 12596-4) and the Quant-iT dsDNA High Sensitivity Assay kit (Invitrogen Q33120 ...
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bioRxiv - Microbiology 2021Quote: ... Clones were screened for correctly assembled DNA fragments by multiplex PCR using the QIAGEN Multiplex PCR kit (QIAGEN) according to the manufacturers’ instructions ...
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bioRxiv - Genetics 2019Quote: ... PCR products corresponding to the 12 mutated codons were pooled and purified with a PCR purification kit (Qiagen). The PCR product was phosphorylated with T4 Polynucleotide Kinase ...
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bioRxiv - Biochemistry 2022Quote: ... and PCR purified (Q5 High-Fidelity 2X MasterMix, New England Biosciences #M0492L; QiaQuick PCR Purification Kit, Qiagen #28104). Single colonies were grown overnight and purified using QIAprep Spin Miniprep Kit (Qiagen #27104) ...
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bioRxiv - Molecular Biology 2019Quote: ... Quantitative PCR was performed using the QuantiTect® SYBR® Green PCR kit (Qiagen Inc., Germantown, MD, #204145) and a CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad Laboratories ...
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bioRxiv - Developmental Biology 2019Quote: ... The gRNA template for in vitro transcription was PCR amplified and purified with Qiaquick PCR purification Kit (Qiagen). In vitro transcription followed and was performed as described52 ...
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bioRxiv - Genetics 2021Quote: Long PCR products were purified by gel electrophoresis and short ones with the QIAquick PCR purification kit (Qiagen). Terminal adenine overhangs were added using Taq polymerase in the presence of dATP ...