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Citations for Qiagen :
2501 - 2550 of 6247 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... Quantitative PCR of the technical replicates was performed using a QuantiFast SYBR Green PCR kit (Qiagen) with the following cycling conditions ...
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bioRxiv - Developmental Biology 2021Quote: Approximately 500bp fragments of axolotl brachyury and sox2 were PCR amplified using OneStep PCR Kit (Qiagen) from RNA extracted from axolotl embryos at various developmental stages using the following primers:
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bioRxiv - Evolutionary Biology 2020Quote: ... Barcoded PCR products were pooled and purified using a QIAquick PCR Purification Kit (Qiagen, Hilden, Germany) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... q-PCR was performed using a Rotor-Gene Q Real-time PCR machine (Qiagen, Hilden, Germany). The oligonucleotides used in PCR were Kdm2b forward ...
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bioRxiv - Developmental Biology 2022Quote: ... CDNA synthesis and qRT-PCR were performed using miRCURY LNA miRNA PCR Starter kit (Qiagen, #339320) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using a MinElute 96 UF PCR Purification Kit (Qiagen, Valencia, CA, USA). Libraries were sequenced (2 x 300 bases ...
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bioRxiv - Genetics 2020Quote: ... Conditions for a 25 μl PCR reaction using the 1 × Taq PCR Master Mix kit (Qiagen) were ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR for microRNA was done following the instructions of miRCURY SYBR Green PCR Kit (Qiagen). Briefly ...
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bioRxiv - Systems Biology 2019Quote: ... PCR products were purified with the QIAquick 96-well PCR Purification Kit (Qiagen, Germantown, MD, USA) and quantified using Qubit dsDNA HS Assay kit (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Amplified PCR products of the selected samples were purified using a QIAquick PCR Purification Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products (250∼280 bp) were purified using a MinElute PCR Purification Kit (QIAGEN, USA) and the libraries were constructed with VAHTS Universal DNA Library Prep Kit for Illumina V3 (Vazyme ...
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bioRxiv - Microbiology 2021Quote: ... both the PCR fragments and the vector were purified with the QIAquick PCR purification kit (Qiagen) and ligated with the T4 DNA ligase (New England Biolabs ...
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bioRxiv - Microbiology 2019Quote: ... Real-time PCR was performed using 2µl of cDNA and QuantiTect SYBR green PCR master (Qiagen) with a LightCycler 96 instrument (Roche Applied Science) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR amplicons from each reaction were purified using a QIAquick PCR Purification kit (Qiagen, Venlo, Netherlands). In vitro transcription to generate dsRNA for each target gene and the negative control was performed by combining reagents of the T7 RiboMAX™ Express RNAi System (Promega ...
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bioRxiv - Immunology 2022Quote: ... PCR products were purified using a MinElute 96 UF PCR Purification Kit (Qiagen, Valencia, CA, USA). Libraries were sequenced (2 x 300 bases ...
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bioRxiv - Genomics 2022Quote: ... Purified PCR products (400 ng) were mixed with 2 μL 10X Taq polymerase PCR buffer (QIAGEN) and nuclease free water to a final volume of 20 μL ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified using a MinElute 96 UF PCR Purification Kit (Qiagen, Valencia, CA, USA). Libraries were sequenced (1 x 300 bases ...
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bioRxiv - Microbiology 2021Quote: ... The PCR mixture contained 1x master mix for the Type-it Microsatellite PCR Kit (Qiagen, France), 3 µL of Q-Solution and 0.25 µM of each primer in a total volume of 30 µL ...
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bioRxiv - Immunology 2022Quote: ... Real-time PCR analysis was further performed on a StepOnePlus Real-Time PCR System from Qiagen using 2x qPCRBIO SyGreen Mix Hi-ROX ...
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bioRxiv - Developmental Biology 2019Quote: PCR products were verified by agarose gel electrophoresis and purified using a PCR Purification Kit (Qiagen). Sanger sequencing was performed on the purified PCR products using the same PCR primers (University of Michigan Biomedical Research DNA Sequencing Core Facility) ...
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bioRxiv - Bioengineering 2019Quote: ... Quantitative real-time-PCR was performed using RT2 Profiler PCR Array for Mouse Mitochondria (330231, Qiagen) with PowerUp SYBR Green (Applied Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR products were combined and isolated using the QiaQuick PCR purification kit (Qiagen, Cat. No. 28106). The quality and concentration of the PCR products was determined using a 2200 TapeStation and D1000 screen tapes (Agilent Technologies) ...
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bioRxiv - Genomics 2020Quote: ... The PCR amplification was carried out in Rotor-Gene-Q Real-Time PCR System (Qiagen, USA) with the following program ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were purified using the MinElute 96 UF PCR Purification Kit (Qiagen, Valencia, CA, USA).
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bioRxiv - Evolutionary Biology 2020Quote: ... Resulting PCR products were then purified using the QIAquick® PCR Purification Kit (Qiagen, Hilden, Germany) before Sanger sequencing by the Sequencing Facility at the Johns Hopkins School of Medicine ...
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bioRxiv - Genomics 2021Quote: ... PCR amplicons were purified using a QIAquick PCR purification kit according to the manufacturer’s instructions (Qiagen) and then they were Sanger sequenced ...
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bioRxiv - Microbiology 2019Quote: ... qRT-PCR was performed to quantify each target transcript using QuantiTect SYBR green PCR kit (Qiagen). The relative gene expression was normalized to the expression of gyrA using the threshold cycle (ΔΔCT ...
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bioRxiv - Microbiology 2019Quote: ... The linear PCR products were then purified with MinElute PCR purification kit (Qiagen, Valencia, CA, USA) and eluted in 10 μl of elution buffer (EB ...
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bioRxiv - Genetics 2020Quote: ... The PCRs were carried-out in 10μl reaction volume following QIAGEN Multiplex PCR Kit (Qiagen, Germany). The thermal cycle profile was ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were purified using a MinElute 96 UF PCR Purification Kit (Qiagen, Valencia, CA, USA). Libraries were sequenced (2 x 300 bases ...
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bioRxiv - Cell Biology 2019Quote: ... DNA was purified after each of the PCR reactions using a QIAquick PCR purification kit (Qiagen). Following the 2 step PCR ...
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bioRxiv - Molecular Biology 2021Quote: ... The residual PCR products were purified using a QIAquick PCR purification kit (Qiagen, Leusden, The Netherlands), and the purified products were sequenced directly at both ends with amplification primers by TaKaRa Biotechnology (Dalian ...
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bioRxiv - Cell Biology 2020Quote: ... All exon specific PCR products were purified using the Qiaquick PCR purification kit (Qiagen, Hilden, Germany) prior to sequencing.
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bioRxiv - Microbiology 2022Quote: ... respectively and PCR products were cleaned using the QIAquick PCR Purification Kit (QIAGEN, Cat. No. 28104). Libraries were sequenced by CGEB-IMR (Centre for Genomics and Evolutionary Biology Integrated Microbiome Resource ...
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bioRxiv - Cell Biology 2022Quote: ... PCR products were pooled and purified over a Qiagen MinElute PCR Purification Kit column (Qiagen, 28004) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... PCR products of the expected size were purified using the QIAquick PCR Purification kit (Qiagen, Germany) and Sanger sequenced in both directions on an Applied Biosystems 3730xl DNA Sequencer (University of California ...
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bioRxiv - Microbiology 2023Quote: ... This was followed by PCR purification using the QIAquick PCR Purification Kit (Qiagen, Cat. No. 28106) and elimination of residual wild-type sequences by enzymatic digestion with DPN1 and Xho1 ...
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bioRxiv - Genetics 2024Quote: ... The 2nd-round PCR product was purified using the QIAquick PCR purification kit (QIAGEN, Hilden, Germany), sequenced and submitted for Synthego Inference of CRISPR Edits Analysis (https://ice.synthego.com/#/) ...
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bioRxiv - Biochemistry 2024Quote: ... PCR arrays were performed using RT2 Profiler™ PCR Array Mouse Cytokines & Chemokines (PAMM-150Z, Qiagen). Calculations were performed by a comparative method (2−ΔΔCt ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR product was purified with the Qia-quick PCR clean up kit (Qiagen, Germantown, MD). The Hi-Scribe T7 High Yield RNA synthesis kit (NEB ...
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bioRxiv - Genomics 2023Quote: ... PCRs of each sample were pooled together and purified using the MinElute PCR Purification Kit (QIAGEN) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... The PCR products were pooled in each group and purified using QIAquick PCR Purification Kit (Qiagen). Sequencing adaptors were added by PCR enrichment of 1 ng of the purified amplicons using forward primer P9 and indexing reverse primer P10 with KAPA HiFi HotStart ReadyMix with the following conditions ...
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bioRxiv - Bioengineering 2023Quote: ... Then the PCR products were purified using the QIAquickR PCR Product Purification Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and PCR products purified and concentrated with the QIAquick PCR Purification kit (Qiagen; cat. no. 28106). PCR products were sent for Sanger sequencing with both forward and reverse primers to Azenta Biosciences ...
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bioRxiv - Pathology 2023Quote: ... Real-time PCR was conducted with cDNA 20 using the Quantitect SYBR Green PCR kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The PCR product was then purified using the QIAquick PCR Purification Kit (Qiagen, Venlo, the Netherlands) and sent to Macrogen Europe (Amsterdam ...
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bioRxiv - Cell Biology 2023Quote: ... PCR amplification was performed with 15 cycles and purified with Qiaquick PCR Purification Kit (Qiagen # 28106). cDNA libraries were then size-selected using AMpure XP beads (Beckman Coulter #A63881) ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 independent PCR reactions were pooled and purified using the QIAquick PCR Purification Kit (Qiagen #28106).
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bioRxiv - Synthetic Biology 2024Quote: ... DNA purification from PCR reaction mixtures was performed using QIAquick PCR Purification Kit (Qiagen Ltd UK). Microbial genomic DNA was extracted using GenElute Bacterial Genomic DNA Kit (Sigma) ...
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bioRxiv - Plant Biology 2020Quote: ... MiniElute PCR Purification Kit (Qiagen, Germany) was used for cleaning ChIP DNA and two rounds of in vitro transcription by T7 RNA polymerase followed according to a linear DNA amplification (LinDA ...