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Citations for Qiagen :
2601 - 2650 of 6247 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 1 x Qiagen PCR Buffer (Qiagen), 3 U APEX Taq (Genesee Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR fragments were column purified (Qiagen) and quantified ...
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bioRxiv - Microbiology 2022Quote: ... column purified (Qiagen PCR Purification kit), assembled with Gibson Master Mix (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR cleanup kit (Qiagen, Hilden, Germany) was used to purify the DNA ...
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bioRxiv - Physiology 2022Quote: ... and PCR Clean-Up Kit (QIAGEN). Purified RACE products were inserted into pRACE vector with In-Fusion DM Master Mix ...
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bioRxiv - Immunology 2024Quote: ... PCR products were gel purified (Qiagen) and sequenced on Novaseq 6000 platform (Illumina) ...
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bioRxiv - Immunology 2024Quote: ... the MinElute PCR Purification Kit (Qiagen) was used according to the manufacturer’s instructions and eluted into 12 μl of elution buffer ...
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bioRxiv - Neuroscience 2023Quote: ... PCR was performed using HotStarTaq (Qiagen) and bands visualized by gel electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... using Quantinova Probe PCR kit (Qiagen) in the presence of Taqman® probes for INO1 (Sc04136910_s1 ...
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bioRxiv - Developmental Biology 2023Quote: PCR products were column purified (Qiagen), and in vitro transcription was performed with Ambion T7 MEGAshortscript kit (Life Technologies) ...
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bioRxiv - Microbiology 2024Quote: ... PCR was performed with Taq (Qiagen) or Phusion (NEB ...
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bioRxiv - Immunology 2023Quote: ... Quantitect SYBR green PCR mastermix (Qiagen) or Power SYBR green mix (Applied Biotechnologies ...
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bioRxiv - Microbiology 2023Quote: ... The QIAquick PCR purification kit (Qiagen) was used to purify the resulting products ...
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bioRxiv - Cancer Biology 2023Quote: ... pooled using PCR purification kit (QIAGEN) and eluted in 85ul PCR-grade water ...
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bioRxiv - Microbiology 2024Quote: ... purified using PCR Cleanup kit (Qiagen), and quantification of DNA concentration using a Qubit HS kit (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... and fragments sized 150-350 bp were excised and then purified using a MinElute Gel Extraction kit (QIAGEN 28604). The purified fragments were treated with sodium bisulfite using an EZ DNA Methylation-Gold Kit (Zymo Research D5005) ...
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bioRxiv - Biochemistry 2022Quote: ... The remaining recovery was grown overnight in 150 mL LB + chloramphenicol and plasmid DNA was isolated by MaxiPrep (Qiagen). The complexity of the SpCas9 catalytic and PAM domain libraries were estimated to be 77,400 and 292,600 respectively ...
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bioRxiv - Cancer Biology 2021Quote: ... Wing imaginal discs or their neoplasms were thus obtained were resuspended in 150-300 μL of RLT Buffer (Qiagen) containing 1.5-3 μL of ◻-mercaptoethanol ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA was lysed with a lysis buffer (10 mM Tris-HCl, pH 8.0, 150 mM NaCl, 20 mM EDTA, 1% SDS) and proteinase K (Qiagen) was added to samples to degrade proteins ...
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bioRxiv - Immunology 2023Quote: ... The periplasmic fraction was filtered with a 0.22 um filter and incubated with 4 mL 50% Ni-NTA resin equilibrated in wash buffer (20 mM HEPES, pH 7.5, 150 mM NaCl, 40 mM imidazole) (Qiagen) per liter of initial bacterial culture ...
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bioRxiv - Plant Biology 2019Quote: ... and RNA was extracted following the manufacturer’s instructions using the RNase-free DNase I Set for on-column DNase treatment (Qiagen, Germantown, MD, USA).
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bioRxiv - Immunology 2021Quote: Total RNA extraction from cells and DNase treatment were performed with the RNeasy Mini kit and RNase-free DNase set (Qiagen, Hilden, Germany). The quality and integrity of total RNA were verified on an Agilent Technologies 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... and from infected cells and hamster tissues with the RNase-Free DNase set coupled with the RNeasy Midi Kit (Qiagen, 79256 & 75144), according to the manufacturers’ protocols ...
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bioRxiv - Cancer Biology 2022Quote: Total RNAs from mouse tumors were extracted and purified using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer ...
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bioRxiv - Genetics 2022Quote: ... followed by DNase treatment with the Qiagen RNase-Free DNase Set and purification by the Qiagen RNeasy Mini Kit (Qiagen, Germantown, MD). RNA samples were confirmed for their quality with the Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Genomics 2022Quote: ... DNA was extracted from pelleted nuclei with the Qiagen Genomic tips 20/G and HMW DNA extraction buffer set (Qiagen no. 19060) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... with a scaled down version of the manufacturer’s protocol with Dnase treatment of RNA using either the kit’s DNase or the Qiagen RNase-Free DNase Set (Qiagen, Valencia, CA, USA). Water controls were performed with each extraction batch ...
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bioRxiv - Pathology 2022Quote: ... Cell lysates were transferred onto RNeasy mini-spin columns and DNA was removed using DNase digestion/treatment using RNase-Free DNase Set (Qiagen, Hilden, Germany.) The RNA Integrity Number (RIN ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA isolation was performed using QIAzol Lysis Reagent and the miRNEasy Mini Kit including the RNAse-free DNase Set (Qiagen, Hilden, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from the plant tissues using the RNeasy Plant Mini Kit and Qiagen RNase-Free DNase Set (Qiagen, Hilden, Germany). Three independent RNA samples from each tissue were used for analysis ...
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bioRxiv - Plant Biology 2023Quote: ... followed by genomic DNA removal and cleaning using Qiagen RNase-Free DNase Set kit (cat#79254) and Qiagen Mini RNeasy™ kit (cat#74104) (Qiagen, Denmark). Integrity of the RNA samples was assessed using the Alegient 2100 Bioanalyzer ...
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bioRxiv - Cancer Biology 2022Quote: ... All RNA samples were treated with RNAse-free DNAse provided by manufacturer’s kits or with RNase-Free DNAse Set (Qiagen, cat. No. 79254). All DNA samples were treated with RNAse provided by manufacturers to remove contaminant RNAs ...
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bioRxiv - Cancer Biology 2023Quote: Total RNAs from mouse tumors were extracted and purified using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... total RNAs were extracted from cells cultured alone or the mixed co-culture using the RNeasy kit and were decontaminated of the DNA using RNase-Free DNase Set (Qiagen, Hilden, Germany). Libraries were prepared by the Van Andel Institute Genomics Core from 500 ng of total RNA using the KAPA stranded mRNA kit (v4.16 ...
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bioRxiv - Immunology 2024Quote: ... fast Gene Set Enrichment Analysis (fGSEA)33 was used for gene set enrichment analysis and additional Ingenuity pathway analysis (IPA) was performed using IPA software (Qiagen, Germantown, MD).
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bioRxiv - Microbiology 2023Quote: ... The RNA samples were resuspended in RNase-free water before clean-up treatment with the RNase-Free DNase Set (Qiagen, Hilden, Germany) following the manufacturer’s protocol for off-column clean-up ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA removal was performed by applying 80 μl of DNase I working solution (10 μL DNase stock + 70 μL 1x RDD buffer; RNase-Free DNase Set, Qiagen, Hilden, Germany) to the column and incubated for 15 min at room temperature ...
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bioRxiv - Genomics 2024Quote: ... DNA was extracted from three female and two male pooled sample sets using the MagAttract High Molecular Weight kit (Qiagen, Valencia, CA) according to the manufacturer’s protocols with the addition of an extra wash step using the provided wash buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA synthesis for miRNA was performed using 200ng RNA and miRscript RT kit II (Qiagen, UK) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA synthesis for miRNA analysis was done with the miRCURY LNA RT kit (Qiagen, cat# 339340) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 200 ng of the total RNA was reverse transcribed using miRCURY LNA RT kit (QIAGEN, 339340). The cDNA libraries were then diluted 80 times and used for the analysis of gene expression by qPCR using miRCURY LNA SYBR Green PCR kit (QIAGEN ...
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bioRxiv - Cancer Biology 2019Quote: ... 500 ng of RNA per sample was reverse-transcribed to cDNA using Omniscript RT kit (QIAGEN) supplemented with RNase inhibitor (QIAGEN ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 μg of RNA was reverse-transcribed into cDNA using the RT Omniscript cDNA kit (Qiagen), and 20 ng of cDNA template was amplified on a LightCycler-480 (Roche ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were subsequently homogenized at RT for 1 minute at 30Hz (Tissue Lyser II, Qiagen). 20ul of each homogenate was removed for protein concentration measurements (Pierce BCA Protein Assay Kit ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription was performed with 0.5 µ g cRNA using the Omniscript RT mini kit (Qiagen). cDNA amplication was carried out with Light-Cycler 480 SYBR Green I Master mix (Roche ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA synthesis was performed with 100 ng of total RNA using miScript Plant RT kit (Qiagen); qRT-PCR assays were done with SYBR Green PCR kit (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA synthesis was performed from 1 μg of total RNA using the Omniscript RT Kit (Qiagen). All the samples were analyzed in triplicate using GoTaq qPCR Master Mix (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... 500 ng of RNA per sample was reverse-transcribed to cDNA using Omniscript RT kit (Qiagen) supplemented with RNase inhibitor (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... cDNA was generated from isolated total RNA samples using a miScript II RT kit (Qiagen, 218161). cDNA was stored at -20°C before qPCR ...
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bioRxiv - Developmental Biology 2021Quote: ... Reverse transcription was performed using the QuantiTect Reverse Transcription (RT) Kit according to manufacturer’s protocol (QIAGEN). Input RNA was determined by the sample with lowest concentration within each experiment ...