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Citations for Qiagen :
2951 - 3000 of 6247 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 100ng of RNA was converted to cDNA for 1h at 37 °C using the Omniscript RT kit (Qiagen) and random primers (Promega) ...
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bioRxiv - Neuroscience 2022Quote: The reverse transcription was performed with 500 ng of total RNA using the miScript RT II kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA for RNA-seq and RT-qPCR was isolated with the help of RNeasy minelute columns (Qiagen), following the addition of 400 μl of 70% RNase-free ethanol to the TRIzol homogenates and on-column DNaseI digests ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was synthesized using 1 µg of RNA per sample according to manufacturer’s instructions (Omniscript RT kit, Qiagen). Taqman assay probes (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... Reverse transcription of small RNAs was performed for all embryonic stages using the miRCURY LNA RT kit (Qiagen) using the recommended 10 ng total RNA input.
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bioRxiv - Neuroscience 2019Quote: ... The cDNA was measured in triplicates by RT-qPCR (reaction volume 15 l) using Rotor-Gene Q (Qiagen) and Brilliant III Ultra-Fast SYBR Green qPCR Master Mix (Agilent) ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was prepared from 1 μg of total RNA using the QuantiTect RT Kit (Qiagen, Venlo, the Netherlands). Quantitative RT-PCR was performed using TB Green Premix ExTaq II (Takarabio ...
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bioRxiv - Developmental Biology 2022Quote: Expression level of Cdc25a mRNA was measured by RT-qPCR using FastLane Cell SYBR Green kit (Qiagen, #216213) using CFX96 Touch Real-Time PCR Detection System ...
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bioRxiv - Bioengineering 2023Quote: ... direct RT-qPCRs were performed on heat-inactivated samples without extraction using the QuantiTect Reverse Transcription Kit (Qiagen). The reactions were run in an Applied Biosystems QuantStudio 7 Flex System (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... An amount of 1 µg of RNA was reverse transcribed using Omniscript Reverse Transcription (RT) Kit (Qiagen, #205113) together with random oligonucleotides (Roche ...
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bioRxiv - Bioengineering 2023Quote: ... The RT-qPCR experiments used Student’s t-test of the experimental group compared to the sham control (Qiagen GeneGlobe RT2 Profiler PCR Data Analysis) ...
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bioRxiv - Cell Biology 2023Quote: RT-qPCR for siRNA validation was performed by extraction of RNA using the RNeasy Mini kit (Qiagen 74104) according to the manufacturer’s recommendations 24hr post siRNA transfection ...
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bioRxiv - Cell Biology 2023Quote: Up to 2 μg RNA was reverse transcribed to cDNA using the Omniscript RT Kit (Qiagen, Manchester, UK) or High-Capacity Reverse Transcription Kit (ThermoFisher ...
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bioRxiv - Genomics 2020Quote: ... 0.4 M NaOH and 10 mM Tris-acetated (pH 7.6) and then hybridized to the sequencing primer in PyroMark annealing buffer (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1x Coral Load, 400 nM of each primer, 160 nM dNTPs and 0.5 unit of Top Taq Polymerase, Qiagen). Subsequently we used 5 μL out of 50 μL macerate extract to amplify the mitochondrial cytochrome C oxidase I (COI ...
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bioRxiv - Genomics 2019Quote: Bisulfite-pyrosequencing was performed to validate DNA methylation data from the Illumina 450K array for two CpG sites using specific primers (Supplementary Table 13) designed using PyroMark Assay Design software 2.0 (Qiagen). DNA methylation was quantified from intensities captured in the PyroMark Q24 pyrosequencer (Qiagen ...
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bioRxiv - Molecular Biology 2019Quote: ... mRNA expression levels were normalized to the housekeeping human TATA-binding protein (TBP) mRNA (RT2 qPCR Primer Assays, Qiagen) in the GAL4 assay and to the human housekeeping hypoxanthine guanine phosphoribosyltransferase (HPRT ...
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bioRxiv - Immunology 2019Quote: ... Transcriptional profiling was performed using the nCounter NHP Immunology Panel (NanoString) and verified by RT2 qPCR Primer Assays (Qiagen) for ISG15 ...
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bioRxiv - Genomics 2020Quote: ... then PCR-amplified using the PyroMark PCR kit using Qiagen’s pyrosequencing primer assays or those designed in-house (Suppl. Table 5) via the PyroMark Assay Design Software 2.0 (Qiagen). Reaction conditions were as follows ...
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bioRxiv - Immunology 2021Quote: ... All data was normalized to input and presented as enrichment over the IGX1A negative control region (primers from Qiagen).
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bioRxiv - Microbiology 2021Quote: The QIAseq SARS-CoV-2 Primer Panel paired with the QIAseq FX DNA Library construction kits (Qiagen GmbH, Germany) were used for enriching and sequencing the entire SARS-CoV-2 viral genome ...
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bioRxiv - Genomics 2021Quote: Libraries were constructed using QIASEQ SARS-CoV-2 Primer Panel and QIAseq FX DNA Library UDI-A kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Second-strand synthesis reaction was carried out in 100 μl using 10 pmol of each primer (HotStarTaq Plus, Qiagen). Residual primers and dNTPs were again degraded enzymatically (ExoSAP-IT ...
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bioRxiv - Molecular Biology 2024Quote: ... QT65005) and 10 µM each of forward and reverse primers (listed in Table S2) on Rotor-Gene Q (QIAGEN) with PCR procedures under the following programme ...
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bioRxiv - Genetics 2023Quote: ... 4 μL PCR mastermix (made up of 0.75 μl at a concentration of 0.2 μM of each primer, 80 μl ultrapure water, and 250 μl QIAGEN Multiplex PCR mix ...
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bioRxiv - Neuroscience 2023Quote: ... OneLμg of total RNA was converted to cDNA using random primers in a volume of 20Lμl using an RT2 HT first stand kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Translocation positive bands were re-amplified from the corresponding first round PCR in second round PCR reactions and purified with QIAquick Gel Extraction Kit (Qiagen) for DNA sequencing.
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bioRxiv - Plant Biology 2020Quote: Quantitative real-time PCR was performed in a volume of 5 mL QuantiTect Probe PCR Kit (Qiagen GmbH, Hilden, Germany) kit and an ABI 7900HT fast real-time PCR system (ThermoFisher Scientific Inc. ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was carried out using a QuantiFast SYBR Green PCR kit (QIAGEN, 204056) on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2020Quote: ... The cDNA (200 ng) was used for quantitative PCR with the Human Cellular Senescence RT2 Profiler™ PCR Array (Qiagen) containing 84 key genes involved in the initiation and progression of the biological process causing cells to lose the ability to divide ...
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bioRxiv - Microbiology 2021Quote: ... 250 ng of cDNA were used as the template for real-time PCR using QUANTITECT SYBR GREEN PCR KIT (QIAgen). Reactions were performed using the ABI 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2020Quote: ... Two gRNAs targeting the first and second coding exons were generated via PCR reaction and purified using QIAquick PCR Purification Kit (QIAGEN). The sequence targets were ...
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bioRxiv - Developmental Biology 2020Quote: ... The PCR products were detected by gel electrophoresis and purified with the MinElute PCR Purification Kit (Qiagen, Valencia, CA, USA) and sequenced.
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bioRxiv - Developmental Biology 2020Quote: ... A second PCR was performed using the same parameters and the products were purified with a QIAquick PCR Purification Kit (Qiagen). Single stranded riboprobes were transcribed with a Roche DIG Labeling Kit (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... Each sample was then amplified by the determined number of PCR cycles and then purified (MinElute PCR Purification Kit, Qiagen). Final libraries were then size selected for fragments between 100-1000bp using AMPure XP beds (Beckman Coulter ...
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bioRxiv - Microbiology 2019Quote: ... Each reaction mix comprised 10 μL of 2x Rotor-Gene Multiplex PCR Master Mix (Rotor-Gene Multiplex PCR Kit, catalogue no. 204774; Qiagen), 1 μL of a mix of primers and probes (to give final concentrations of 0.5 mM each primer and 0.2 mM each probe) ...
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bioRxiv - Synthetic Biology 2019Quote: ... Amplification was analysed in a 1.5 % (w/v) agarose gel and purified from the PCR reaction using the QIAquick PCR Purification Kit (Qiagen, #28104), and subsequently cloned in to the pMini-T plasmid using the PCR Cloning Kit (NEB #E1202) ...
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bioRxiv - Genetics 2020Quote: ... Because of clear evidence of the response of the pGL4.74 Renilla normalisation control plasmid to PMA treatments and co-transfection with the EGR1 expression vector Lucia activity levels were normalised against quantitative PCR (qPCR) of levels of Lucia gene DNA present in cell extracts recovered using Qiagen PCR cleanup kits (Qiagen). Lucia luciferase activity was normalised against the crossing point (Cp ...
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bioRxiv - Synthetic Biology 2020Quote: All nicked PCR products (obtained either via PfAgo or Cas9n reactions) were purified using the Qiaquick PCR purification kit (QIAGEN) and eluted in ddH2O ...
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bioRxiv - Immunology 2021Quote: ... The PCR products were subjected to 1 % agarose gel electrophoresis for analysis and purified with QIAquick PCR Purification Kit (Qiagen) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: ... A total of 25 cycles of PCR was performed and the expected PCR products (500-600 bp) were gel purified (Qiagen). NGS was performed on the Ion S5 GeneStudio system ...
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bioRxiv - Immunology 2021Quote: ... A total of 25 cycles of PCR was performed and the expected PCR products (500-600 bp) were gel purified (Qiagen). NGS was performed on the Ion S5 GeneStudio system ...
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bioRxiv - Microbiology 2020Quote: ... Real-time quantitative PCR was performed using Mx3000pTM SYBR Green real-time quantitative PCR Analyzer (QIAGEN Biotechnology Co, Ltd, China). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... mexicana Cas9 T7 procyclic promastigotes were transfected either with whole PCR reactions or 5 µL of DNA purified using the QIAquick PCR Purification Kit (Qiagen). 8 x 106 log phase cells were prepared by spinning down (1,000 x g for 10 min) ...
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bioRxiv - Cancer Biology 2021Quote: ... Correct and unique amplification of the target regions was verified by agarose gel electrophoresis before purifying PCR products using the QIAquick PCR Purification Kit (Qiagen). For analysis by TIDE ...
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bioRxiv - Genomics 2020Quote: ... followed by evaluation of the PCR products by gel electrophoresis and purification with the Qiaquick PCR purification kit (28104, Qiagen). These PCR primers were evaluated for specificity in human (MCF7 ...
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bioRxiv - Bioengineering 2022Quote: DNA templates for generating desired RNA products were created by PCR amplification from plasmids or gBlock gene fragments (IDT) and purified using a PCR purification kit (Qiagen). Plasmids were then generated with these templates containing a T7 promoter followed by 5’ ribozyme sequence ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The vector pPROEX HTa was restricted with Nco1 and Not1 and gel purified before being ligated to Pci1-Not1 restricted PpMurE_L63 or Bsa1-Not1 restricted AnMurE PCR fragments that had been cleaned up with a PCR clean up kit (Qiagen). Coding sequences were confirmed by Sanger sequencing (Eurofins).
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bioRxiv - Developmental Biology 2021Quote: Expression of 6 selected miRNAs was re-analyzed with an independent cohort by qRT-PCR using the miRCURY LNA miRNA PCR system (QIAGEN). The 6 selected (hsa-miR-144-3p ...
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bioRxiv - Immunology 2020Quote: ... Equal volumes of barcoded PCR2 products (5 μL each) were pooled and PCR column purified using QIAquick PCR Purification Kit (Qiagen). Libraries were quantified using KAPA Library Quantification Kit for Illumina Platforms (Kapa Biosystems) ...