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Citations for Qiagen :
2901 - 2950 of 6247 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... a MinElute PCR Purification kit from Qiagen was used for cleaning up the samples and eluted in 40 µL EBT buffer (1 mM EDTA ...
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bioRxiv - Biochemistry 2023Quote: ... A QIAcuity EvaGreen (EG) PCR Kit (QIAGEN) was used with gene-specific primers for DDR2 and GAPDH ...
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bioRxiv - Developmental Biology 2023Quote: ... purified using QIAquick PCR Purification Kit (Qiagen) and diluted to 500 ng/μl ...
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bioRxiv - Immunology 2023Quote: ... The Qiagen MiniElute PCR purification kit (Qiagen) was used to purify the transposed DNA ...
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bioRxiv - Immunology 2023Quote: ... Nested PCR using HotStarTaq (Qiagen, Hildent, Germany) was then used amplify individual heavy and lambda/kappa light chains from the RT-PCR product ...
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bioRxiv - Genomics 2023Quote: The resulting amplicons were PCR-purified (Qiagen) and cloned into the library vector via Golden Gate cloning with Esp3I (Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... the MiniElute PCR purification kit (Qiagen, 28004) was used to purify the samples ...
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bioRxiv - Genetics 2024Quote: ... purified using MiniElute PCR Purification kit (Qiagen) and subjected to deep 2 x 150 bp paired-end next generation sequencing (NGS ...
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bioRxiv - Cancer Biology 2024Quote: ... 1x PyroMark PCR Master Mix (Qiagen, #978703), 1x CoralLoad Concentrate (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: ... MinElute PCR Purification Kit buffer PB (Qiagen) was added to stop the reaction and tagmented DNA was purified and eluted in 10 μl of EB ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 5 µL Multiplex PCR Master Mix (QIAGEN) and a primer mix (each primer at a final concentration of 0.2 µM) ...
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bioRxiv - Synthetic Biology 2024Quote: ... miRCURY SYBR Green PCR Kit (339345, QIAGEN), miRCURY LNA miRNA PCR Assay (339306 ...
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bioRxiv - Synthetic Biology 2024Quote: ... miRCURY LNA miRNA PCR Assay (339306, QIAGEN), miRCURY LNA miRNA Custom PCR Assay (339317 ...
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bioRxiv - Microbiology 2024Quote: ... purified by MinElute PCR Purification Kit (Qiagen) and mixed in an equimolar amount according to the concentration measured on the Qubit 2.0 Fluorometer (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2020Quote: ... We purified the digested vector from a 0.8% agarose gel (1.2 g agarose Fisher #21-255-00GM) in 150 mL 1x TAE (Fisher #FERB49)) using a QiaQuick Gel Extraction Kit (Qiagen #28706) and quantified using a Qubit dsDNA HS Assay Kit.
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from 150 mg of cluster roots (3 to 4 roots) using the RNeasy Plant Mini Kit (Qiagen, 74904) and treated with the DNA-free DNA Removal Kit (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: We extracted DNA by adding 3-5 mg of tail tissue to 150 µl of 10% ChelexR resin (Bio- Rad, Inc.) with 1.4 µl of Proteinase K (20 mg/ml, Qiagen, Chatsworth, CA), incubating at 55°C for 180 min ...
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bioRxiv - Cancer Biology 2023Quote: MiR libraries were generated from 100 -150 ng of cell using the QIAseq miRNA Library Kit according to the protocol (Qiagen, Germany). Library quality control and concentrations were determined using a High Sensitivity D1000 ScreenTape with TapeStation Analysis Software A.02.02 (Agilent Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR products were purified using the QIAquick PCR Purification Kit according to manufacturer’s instructions (QIAGEN Ltd., Manchester, England). MiSeq™ was performed in-house as described in [25].
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bioRxiv - Developmental Biology 2019Quote: ... PCR fragments were analyzed on agarose gel and concentrated by either PCR clean-up or gel purification kit (QIAGEN) and their concentrations measured using a Nanodrop (Eppendorf ...
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bioRxiv - Immunology 2019Quote: ... Antiviral transcriptional responses were confirmed by way of qRT-PCR using a rhesus macaque RT2 Profiler PCR Array (Qiagen). Responses within each species and treatment group were analyzed together to identify expression levels at the indicated timepoints relative to pre-infection ...
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bioRxiv - Plant Biology 2019Quote: ... All qRT-PCR reactions were performed using SYBR Green I (QuantiFast® SYBR® Green PCR Kit, Qiagen, Germany) chemistry and ARF (ADP-Ribosylation Factor ...
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bioRxiv - Immunology 2021Quote: ... was then added to a polymerase chain reaction (PCR) mix containing 12.5 μl of 2× Multiplex PCR mix (Qiagen), 9 μl of H2O ...
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bioRxiv - Microbiology 2020Quote: ... Relative real-time quantitative PCR was performed with a miScript SYBR Green PCR kit (QIAGEN Biotechnology Co, Ltd, China). The reaction solution was prepared on ice and comprised 10 μL of 2× QuantiTect SYBR Green PCR Master Mix ...
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bioRxiv - Molecular Biology 2020Quote: ... The three replicate PCR products for each sample were pooled and purified using the MinElute PCR Purification Kit (Qiagen). Negative control libraries were also prepared from different stages of the experimental process (extraction ...
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bioRxiv - Microbiology 2022Quote: ... Real-time PCR was performed using RT2 Profiler PCR array in combination with RT2 SYBR green master mix (Qiagen) using a QuantStudio 6 Flex real-time PCR system ...
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bioRxiv - Neuroscience 2020Quote: ... Quantitative PCR was performed using a Q-Rex thermal cycler using the Rotor-Gene SYBR green PCR kit (Qiagen). Primer sequences were ...
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bioRxiv - Microbiology 2020Quote: ... 5 μl of cDNA was then added to a PCR mix containing 12.5 μl 2× Multiplex PCR mix (Qiagen), 9 μl H2O ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were confirmed by agarose gel electrophoresis using 0.8-1% agarose and purified using PCR purification kit (Qiagen). In case of NS1 gene ...
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bioRxiv - Genetics 2022Quote: ... PCR product was pooled and purified two times with a QIAquick PCR Purification Kit (Qiagen, Inc., Valencia, CA, USA) and DNA quantity was measured on an Agilent 2200 TapeStation (Agilent Technologies ...
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bioRxiv - Microbiology 2019Quote: ... miScript miRNA PCR array of Human Neurological Development and Disease was performed using miScript SYBR green PCR kit (Qiagen). The PCR array contains 84 miRNAs which are differentially expressed during neuronal development and are responsible for progression of neurological diseases.
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bioRxiv - Biochemistry 2020Quote: ... was PCR amplified from plasmid (pSH515) containing the native His4 gene promoter and purified by PCR cleanup kit (Qiagen). DNA was eluted from the spin column with 10 mM Tris-HCl ...
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bioRxiv - Genetics 2020Quote: ... and the target sites were PCR amplified by nested PCR amplification and purified by a Gel Extraction Kit (QIAGEN) for deep sequencing.
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bioRxiv - Genetics 2020Quote: ... and the target sites were PCR-amplified by nested PCR amplification and purified by a Gel Extraction Kit (QIAGEN) for deep sequencing.
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription and quantitative real-time PCR were performed with the QuantiFast SYBR Green PCR Kit (Qiagen, Hilden, Germany). Since the endogenous retrovirus envelope comprises only one exon ...
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bioRxiv - Genomics 2022Quote: ... The two PCR reactions for each sample were pooled and cleaned up using the QIAquick PCR Purification Kit (Qiagen) and samples eluted in 15μL Elution Buffer (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: ... The PCR product was separated using 1.5% agarose gels and purified using the QIAquick PCR & Gel Cleanup Kit (Qiagen). Purified PCR products (300 ng ...
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bioRxiv - Microbiology 2023Quote: ... and PCR products were purified and concentrated to 500 ng/uL using a MinElute PCR product purification kit (Qiagen). Strains containing pKD46 were grown at 30 °C ...
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bioRxiv - Immunology 2023Quote: ... Illumina adapters were completed by a second PCR carried out with the Qiagen Multiplex PCR kit (Qiagen, Valencia, CA), using 1 μl of the first PCR product as the template in a 20 μl reaction with the following program ...
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bioRxiv - Microbiology 2023Quote: ... DNA fragments that were generated by the polymerase chain reaction (PCR) were purified using the PCR Purification Kit (Qiagen), Monarch® PCR & DNA Cleanup Kit (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR reactions for each sample were pooled and cleaned up with the QIAquick PCR purification kit (Qiagen 28104). Using the pooled PCR1 products ...
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bioRxiv - Molecular Biology 2023Quote: ... The excess dsDNA was removed by 3 rounds of PCR clean up with QIAquick PCR Purification Kit (Qiagen, 28106).
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bioRxiv - Plant Biology 2023Quote: ... The prepared libraries were enriched using 15 cycles of PCR and purified by the QIAquick PCR purification kit (Qiagen). The Agilent 2100 Bioanalyzer was used to control the size and purity of the samples using the Agilent High Sensitivity DNA Kit ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were assessed on a gel and purified using the Qiaquick PCR Cleanup kit (Qiagen, cat. no. 28104). Plasmids for Foxd-3 and Sox10 mRNA probe synthesis were from C ...
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bioRxiv - Genetics 2024Quote: ... The four PCR replicates obtained from each sample were pooled and concentrated with the MinElute PCR Purification Kit (Qiagen) before gel electrophoresis.
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bioRxiv - Cell Biology 2022Quote: ... Reverse transcription (RT) of total RNA was performed using QuantiTect Reverse Transcription Kit according to manufacturer’s instructions (Qiagen). cDNA was then amplified using TaqMan® Gene Expression Master Mix (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... cDNA was analyzed by RT-qPCR using a Qiagen RT2 profiler custom panel (Qiagen, PAHS-181Z, CLAM3313, CLAH36077) following supplier instructions ...
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bioRxiv - Neuroscience 2020Quote: ... A cDNA library was created form each mRNA sample using the Omniscript RT kit (205113, Qiagen, Valencia CA) with random nonamers (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2019Quote: ... Two hundred to 500 ng of RNA were transcribed with the RT² First Strand Kit (SA Biosciences, Qiagen). Synthetized cDNA was subjected to a PCR array specific for the human antiviral response (RT² Profiler PCR array – PAHS-122Z ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by phenol/chloroform extraction and 1 μg of RNA was reverse transcribed with Quantitect RT kit (Qiagen) following manufacturer’s instructions ...