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Citations for Qiagen :
2301 - 2350 of 6247 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... DNA was simultaneously PCR amplified from each sample using a multiplex PCR kit (Qiagen), with specific target-amplification (STA ...
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bioRxiv - Genomics 2023Quote: ... PCR products were cleaned with a QIAquick® PCR Purification Kit (QIAGEN, Hilden, Germany) and ligated into the pJET1.2/blunt cloning vector using the CloneJET PCR Cloning Kit (ThermoFisher SCIENTIFIC ...
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bioRxiv - Immunology 2023Quote: ... PCR products were visualized on gel and purified using MinElute PCR Purification kit (Qiagen) as per fabricator instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The PCR products were cleaned using a QIAquick PCR purification kit (Qiagen, Germantown, MD). Concentration measurement of nucleic acids was performed using a NanoDrop One UV-vis spectrophotometer ...
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bioRxiv - Zoology 2023Quote: ... target sites were PCR amplified (using the Taq PCR Master Mix produced by Qiagen), and candidate mutations were identified using Sanger sequencing (performed by Azenta Life Sciences) ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR product was purified with QIAquick PCR Purification Kit (Qiagen, Cat. No. 28104), and samples were sent to Eurofins for sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... PCR purification was performed with the QIAquick PCR Purification Kit (Qiagen, cat no: 28106) according to provided protocol ...
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bioRxiv - Bioengineering 2024Quote: ... The PCR products were subsequently purified using the QIAquick PCR Purification Kit (Qiagen, Germany). In vitro transcription was performed overnight at 37 °C with the MEGAshortscriptTM T7 Transcription Kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: A human mitochondrial energy metabolism PCR array (Qiagen RT2 Profiler PCR Arrays, PAHS-008YA) was performed from cDNA synthesized from 1 µg of total RNA from the tumors of control and CPI-613 treated mice ...
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bioRxiv - Genetics 2024Quote: ... PCR products were purified using a spin column (QIAquick PCR Purification Kit, Qiagen #28104), and then used as a template for a second PCR reaction using primers to add the Illumina adaptors and indexes ...
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bioRxiv - Synthetic Biology 2024Quote: ... The PCR-amplified templates were purified (QIAquick PCR purification kit, Qiagen, catalog no. 28106) and verified for the presence of a single DNA band of expected size on a 2% Tris-Acetate-EDTA–agarose gel ...
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bioRxiv - Genetics 2024Quote: ... and PCR amplified at the age-associated regions with the PyroMark PCR kit (Qiagen). 10μl of PCR-product and 2µl of sequencing primer (4μM ...
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bioRxiv - Plant Biology 2024Quote: ... The qRT-PCR experiments were performed using Quantinova SYBR Green PCR kit (Qiagen, 208052) in a 7500 Fast Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were then pooled and purified with a QIAquick PCR Purification Kit (Qiagen), using water to elute from the final columns ...
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bioRxiv - Cancer Biology 2021Quote: ... Gene set enrichment analysis overlap and activity z-scores p values have been calculated using the Ingenuity Pathway Analysis Software (Qiagen) using the previously described method (76) ...
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bioRxiv - Developmental Biology 2021Quote: DNA was digested from aliquots from the same RNA extracted as described above (RNA extraction and smallRNA library preparation) with RNase-Free DNase Set (Qiagen) according to kit protocol and concentrated using Oligo Clean & Concentrator (Zymo research ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA extraction was performed following detailed protocol of the same kit and DNase-treated with RNase-Free DNase Set (Qiagen). RNA concentration and quality were assessed by Bioanalyzer RNA 6000 Pico Assay (Agilent) ...
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bioRxiv - Molecular Biology 2021Quote: ... Significant probes were defined as p < 0.01 and resultant gene sets were uploaded to Ingenuity Pathway Analysis (IPA; Qiagen, Inc.) for probe set annotations and to query relationships between genes ...
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bioRxiv - Immunology 2019Quote: ... Gene set enrichment analysis was done using QIAGEN’s Ingenuity® Pathway Analysis software (IPA®, QIAGEN Redwood City,www.qiagen.com/ingenuity) on genes that passed nominal p<0.05 using “Canonical Pathways” and “Upstream Regulators” options ...
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bioRxiv - Developmental Biology 2019Quote: Total RNA was extracted from FL and HL bud samples using the RNeasy Mini Kit with RNase-Free DNAse Set and a TissueLyser LT (Qiagen). For each sample ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA for chromium sequencing (10x Genomics) was extracted from blood stored in SET buffer using a MagAttract HMW DNAkit (Qiagen) at Scilifelab ...
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bioRxiv - Cancer Biology 2020Quote: Mono- and co-cultures were set up as previously described.34 Total RNA was extracted following 24 h of co-culture using RNeasy kit (Qiagen) with QIAshredders (Qiagen ...
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bioRxiv - Plant Biology 2021Quote: Plant samples of ∼50 mg were frozen in liquid nitrogen and stored at −80°C prior to RNA extraction with RNeasy Plant Mini Kit and on-column RNase-Free DNase Set (Qiagen). Total RNA (0.5 µg ...
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bioRxiv - Neuroscience 2021Quote: ... Genomic DNA contamination was eliminated using either TURBO DNA-free kit (AM1907) or on-column digestion with RNAse-free DNAse set (Qiagen), respectively ...
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bioRxiv - Genomics 2021Quote: ... The flow-through was discarded and on-column DNase digestion was performed using the RNase-Free DNase Set (Qiagen, 79254) and the following protocol from Qiagen ...
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bioRxiv - Plant Biology 2022Quote: RNA extraction and DNAse treatment for transcriptome analysis were performed using the RNeasy Plant Mini Kit and RNase-Free DNase Set from Qiagen. Extracted RNA was quantified using NanoDrop ND1000 and its quality was determined in a Bioanalyzer 2100 (Agilent).
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bioRxiv - Systems Biology 2022Quote: ... cells in the first set were pelleted for genomic DNA (gDNA) extraction using the DNeasy Blood and Tissue Kit (Qiagen) per manufacturers’ instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... substances that did not contain training data sets for model building (Table 1) were further explored using Ingenuity Pathway Analysis (IPA) (QIAGEN Inc. ...
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bioRxiv - Microbiology 2022Quote: RNA extracted from mouse lungs as described above was cleaned up using On-Column DNAse using RNAse-Free DNase Set (Qiagen) and RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2020Quote: ... Total RNA was extracted from individual filter sets (n = 2 per timepoint) using Qiagen RNeasy Mini Kit (Qiagen, Hilden, Germany) as in Harke et al ...
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bioRxiv - Biochemistry 2020Quote: A cell pellet corresponding to 20-25 OD600 units was lysed with glass beads and RNA was isolated from the cell lysate using the RNeasy Mini Kit and RNase-free DNase Set (both Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... RNA was collected using Qiagen RNeasy Mini Kits with Qiashredder and on-column DNase I digestions with Qiagen RNase-free DNase Set (Qiagen).
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bioRxiv - Plant Biology 2019Quote: RNA from 3 biological replicates from each experiment was extracted from shoots using an RNeasy Mini Kit with RNase-free DNaseI Set (QIAGEN) and quantified on both a Nanodrop 1000 spectrophotometer (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and RNA was extracted using RNeasy Mini Kit and DNAse treated using the RNase-Free DNase Set (Qiagen, Hilden, Germany). The quantity and quality of the RNA was assessed using the Qubit (Qubit RNA BR Assay Kit ...
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bioRxiv - Genetics 2021Quote: ... We later extracted total RNA from each tissue sample using the RNeasy Mini Kit with RNase-Free DNAse Set and a TissueLyser LT instrument (Qiagen). RNA-sequencing libraries were prepared and sequenced by the High-Throughput Genomics and Bioinformatic Analysis Shared Resource at the University of Utah ...
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bioRxiv - Cancer Biology 2020Quote: The list of transcripts differentially expressed in Huh7 and Huh7-GCK+/HK2− cell lines was analyzed by gene set enrichment analysis (IPA, Build version: 486617M, Qiagen) weighted by their corresponding fold-change and p value ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was extracted from a set of different organs and developmental stages using the RNEasy Plant Mini kit (Qiagen, Netherlands) with the modifications described previously (Logacheva et al. ...
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bioRxiv - Biochemistry 2021Quote: Genomic DNA was isolated from 2 clones of each strain corresponding to 50 OD600 units with the Genomic DNA Buffer Set and Genomic-tip 100/G (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... An on-column DNase digestion step was also performed under the same instructions using an RNase-Free DNase Set (79254, QIAGEN). Final RNA concentration and sample purity were determined via a NanoDrop 1000 spectrophotometer (Thermo Fisher).
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bioRxiv - Genomics 2021Quote: ... RNA was extracted using a QIAGEN RNeasy Mini Kit followed by two rounds of DNase digestion: one on-column using the QIAGEN RNase-Free DNase Set (79254, QIAGEN); and one off-column using Invitrogen’s TURBO DNA-free Kit (AM1907 ...
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bioRxiv - Microbiology 2021Quote: ... and on-column DNA digestion was performed using Qiagen RNase-Free DNase Set according to manufacturer’s protocol (Qiagen, Valencia, CA). The RNA purity was tested by measuring 260 nm/280 nm and 260 nm/230 nm ratios in TE buffer and the values should be over 1.8 ...
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bioRxiv - Immunology 2021Quote: ... RNA was isolated using the Innuprep RNA minikit 2.0 0 (Jena Analytik, Germany) with an additional DNAse digestion step (RNase-free DNase set; Qiagen, Germany). Quality and quantity of the total RNA samples were evaluated with the Tape Station 2200 (Agilent ...
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bioRxiv - Microbiology 2020Quote: ... DNA extraction suitable for PacBio SMRT sequencing was performed using the Genomic DNA Buffer Set (Qiagen Inc, Germantown, Maryland, USA) (Supplementary method 2) ...
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bioRxiv - Molecular Biology 2021Quote: Total RNAs were extracted from NPC cell pellets using the Qiagen RNeasy plus mini kit and treated with DNase1 using the RNase-free DNase set (Qiagen), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Differentially expressed genes were investigated for overlap with published datasets using Gene Set Enrichment Analysis (GSEA) and upstream regulators were predicted using Ingenuity Pathway Analysis (IPA) software (Qiagen).
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was transferred to QIAshredder columns and RNA was isolated using RNeasy Mini Kit and RNase-Free DNase set (Qiagen) following the manufactures protocol ...
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bioRxiv - Genomics 2022Quote: ... the genomic DNA of the Nichi01 strain was isolated from the silk glands of two male fifth-instar larvae using Genomic-tips 100/g and Genomic DNA Buffer Set (QIAGEN). Long-read sequencing for genome assembly was performed using continuous long-read (CLR ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was extracted using the PicoPure RNA Extraction kit (Arcturus) and treated with the RNase-Free DNase Set (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Pathway enrichment analysis was performed using gene sets and pathways defined in the Ingenuity Pathway Analysis software (Qiagen, Germantown, MD) and the ClusterProfiler package16 ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was purified using the RNeasy Minikit and DNase-treated at room temperature for 15 min using the RNase-free DNase set (Qiagen). cDNA was synthesized using the Maxima H Minus cDNA synthesis master mix (Thermo Scientific) ...