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Citations for Qiagen :
2451 - 2500 of 6247 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... All PCR products and enzyme-digested vectors were purified using QIAquick PCR Purification Kit (Qiagen), and the resulting vectors were transformed into ECOSTM Competent E ...
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bioRxiv - Microbiology 2023Quote: ... The three PCR products were purified with the QIAquickTM PCR purification kit (Qiagen, Hilden, Germany). The fusion PCR was performed with the nested primers UPC2_del_PF4 and UPC2_del_PR4 in the presence of 1.3 M betaine.
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bioRxiv - Cancer Biology 2023Quote: ... Multiplex PCR was performed for each probe group using the EpiTect MethyLight PCR Kit (Qiagen) in the following conditions ...
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bioRxiv - Immunology 2024Quote: ... we purified the A-tailed PCR product with a QIAquick PCR Purification Kit (QIAGEN, 28104), followed by subcloning into the pGEM-T-Vector according to the manufactures protocol ...
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Development and Initial Characterization of Pigs with DNAI1 Mutations and Primary Ciliary DyskinesiabioRxiv - Physiology 2024Quote: ... The resulting PCR products were purified using the QIAquick PCR Purification Kit (Qiagen, Hilden, Germany), cloned into the TOPO TA vector ...
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bioRxiv - Microbiology 2020Quote: RNA extraction was performed from 150 µl of ED cell supernatant using the QIAamp viral RNA kit (Qiagen). EAV genome copy number was determined by one step RT-qPCR using the QuantiTect™ Virus + ROX Vial Kit (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: ... 150 μL of the aggregated or non-aggregated cells were then washed with 2 volumes of RNAprotect (Qiagen) to prevent RNA degradation ...
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bioRxiv - Physiology 2019Quote: ... and cDNA synthesis (microRNA) was performed using 100ng RNA and miRscript RT kit II (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2019Quote: ... and cDNA was reverse-transcribed using the miRCURY LNA RT Kit (Qiagen, cat. no. 339340) according to the manufacturer’s instructions on an Applied BioSystems ProFlex PCR System ...
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bioRxiv - Bioengineering 2021Quote: ... 0.3 μg of total RNA was reverse-transcribed using the RT First-strand Kit (Qiagen). The cDNA obtained in solution was mixed with Power SYBR Green PCR MasterMix (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... cDNA synthesis was performed using the Qiagen Sensiscript RT kit (cat# 205213, Qiagen, Germantown MD) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... and the extracted RNA was converted into cDNA using the Omniscript RT kit from Qiagen. Subsequent PCR was performed with primers (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA synthesis was conducted on 500 ng of total RNA using Omniscript RT Kit (Qiagen). The cDNA was diluted 1:2 with water and 0.5 µL was used as template in each RT-PCR reaction ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.75 µg total mRNA was used to generate cDNA using the Quantitect RT kit (Qiagen). 2 µl cDNA was combined with 25 µl 2× TaqMan Gene Expression Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 RNA was quantified by RT-qPCR with the QuantiTect Kit (Qiagen, 204443) with Sarbeco E gene primers and probe in a QuantStudio 3 thermocycler (Applied Biosystems) ...
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bioRxiv - Genetics 2021Quote: ... by mixing 1 μl of RT product with 10 μl of SYBR qPCR Mastermix (Qiagen) containing the appropriate primers (345 nM of forward primer and 345 nM of reverse primer) ...
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bioRxiv - Neuroscience 2022Quote: ... 50ng of RNA was reverse transcribed into cDNA using the miRCURY LNA RT kit (Qiagen). RT-qPCR mix was prepared using the miRCURY LNA SYBR Green PCR kit (Qiagen) ...
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bioRxiv - Plant Biology 2022Quote: ... prior to cDNA synthesis from 500 ng of total RNA using Omniscript RT Kit (Qiagen). The cDNA was diluted 1:2 with water and 0.5 μL was used as template in each RT-PCR reaction ...
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bioRxiv - Physiology 2024Quote: ... RT-qPCR reactions were carried out in triplicate with QuantiFast SYBR Green Master Mix (Qiagen) at an annealing temperature of 60ºC ...
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bioRxiv - Neuroscience 2023Quote: ... ISRIB was withdrawn for 24 hours prior to cell lysis in RT lysis buffer (Qiagen). RNA extraction using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... Complementary DNA was synthesized from 1 μg of RNA using the QuantitTect RT kit (Qiagen). Quantitative PCRs (qPCR ...
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bioRxiv - Neuroscience 2023Quote: ... an aliquot of RNA (500 ng) was reverse transcribed using the Ominiscript RT kit (Qiagen). Real-time PCR quantification was performed using Sybr green and primers specific for the gene of interest ...
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bioRxiv - Cancer Biology 2024Quote: ... 50ng of RNA was reverse transcribed into cDNA using the miRCURY LNA RT kit (Qiagen). RT-qPCR mix was prepared using the miRCURY LNA SYBR Green PCR kit (Qiagen) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1ug of small RNA was used for cDNA preparation using miscript II RT kit (Qiagen) as per the manufacturer’s instructions and profiled the most abundant 1092 miRNAs using human mirnome miscript miRNA PCR array (Qiagen) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cleared lysates were applied to RNeasy silica columns and on-column DNase digestion was performed using RNase-Free DNase Set (QIAGEN, 79254) to remove DNA in silica membrane ...
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bioRxiv - Immunology 2019Quote: ... in which buffer RLT was supplemented with β-mercaptoethanol and displaced on-column DNase treatment using a Qiagen RNase-Free DNase Set (Qiagen, Hilden ...
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bioRxiv - Plant Biology 2021Quote: ... minor using TRIzol Reagent (Thermo Fischer Scientific, Waltham, MA, USA) and treated with the RNase-Free DNase Set (Qiagen, Hilden, Germany) in accordance with the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: RNA was extracted from 30 mg placental samples using the miRNeasy mini kit with the RNase-free DNase Set (Qiagen, UK) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Downstream pathway analysis was performed using the gene set enrichment analysis (GSEA) tools provided by the Broad Institute 32 or ingenuity pathway analysis (IPA) (QIAGEN Inc.) 35.
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bioRxiv - Immunology 2021Quote: ... according to the product instructions and underwent 15 min on-column DNase digestion using an RNase-Free DNase Set (Qiagen, 79254). cDNA was prepared with reverse transcriptase using iScript kit (BioRad Laboratories ...
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bioRxiv - Immunology 2021Quote: ... and ImmunoCult™ Human Treg Differentiation Supplement (StemCell TechnoRNA was isolated at set time points after activation using the RNeasy Mini (Qiagen). cDNA was generated using the High-Capacity RNA-to-cDNA Kit (ThermoFisher) ...
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bioRxiv - Neuroscience 2021Quote: ... The isolated RNA was eluted in 15 μl of elution buffer following on column DNAse1 treatment using RNase-free DNase set (Qiagen # 79254). The total RNA was assessed for RNA integrity with a bioanalyzer (Agilent ...
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bioRxiv - Molecular Biology 2022Quote: ... The pellet was rinsed with 70% ethanol and dried at room temperature before dissolving in 100 μL of RNase-free DNase set (Qiagen 79254) and incubated at room temperature for 10 minutes to remove DNA ...
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bioRxiv - Plant Biology 2022Quote: ... an additional on-column DNase Digestion with the RNAse-Free DNase Set was performed according to the manufacturer’s protocol (Qiagen, Hilden, Germany). The integrity of the total RNA extracted was confirmed with the TapeStation 2200 using RNA screen tape (Agilent Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... 30µL of DNase and RDD buffer (1:7 ratio) containing solution from the RNase-Free DNase Set (Qiagen, Catalog No. 79254) was incubated on the columns at room temperature for 15 minutes before a second and third round of RNA was performed as well as a final spin to ensure the column was dry ...
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bioRxiv - Genomics 2024Quote: ... Batches of approximately 20 individuals were transferred to a 1.5-mL microcentrifuge tube with 100 µL of G2 Buffer (lysis buffer from Genomic DNA Buffer Set, Qiagen ID: 19060) and carefully crushed with a plastic pestle less than four hours after their collection.
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bioRxiv - Plant Biology 2023Quote: ... and DNA contamination was removed using the on-column DNase treatment of the RNase-Free DNase Set (Qiagen GmbH, Hilden, Germany). Ribosomal RNA was depleted by poly A enrichment ...
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bioRxiv - Genomics 2020Quote: ... together with 0.2 uM of each biotinylated primer and 0.25 units of HotStarTaq PlusDNA Polymerase (Qiagen). Refer to Supplementary Table 10 for primer sequences ...
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bioRxiv - Immunology 2021Quote: Pathway-specific primer mixes (Rat Antibacterial Response, PBR-148Z, and Human Antibacterial Response, PBH-148Z; Qiagen) were used for preamplification and qPCR arrays (Rat Antibacterial Response ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... RT2 SYBR Green ROX qPCR Mastermix and primers for Gapdh were purchased from Qiagen (Valencia, CA). The threshold cycle (Ct ...
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bioRxiv - Microbiology 2021Quote: ... The following primer pairs were used and data were analyzed with Rotorgene 6000 Series Software (Qiagen): GAPDH ...
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bioRxiv - Cell Biology 2021Quote: ... Real time qPCR was performed using SYBR Green detection and gene specific QuantiTect Primer Assays (Qiagen) on a 7900HT Applied Biosystems analyser ...
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bioRxiv - Genetics 2019Quote: ... The primers of the four CpGs were designed using the software PyroMark Assay Design by QIAGEN. DNA samples were converted by bisulfite reaction performed on EZ-96 DNA Methylation-Gold™ MagPrep kit provided by ZYMO RESEARCH CORP ...
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bioRxiv - Cell Biology 2019Quote: ... was used to detect OASL expression together with a QuantiTect® primer assay (Qiagen, Limburg, Netherlands) for OASL (Cat ...
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bioRxiv - Microbiology 2019Quote: ... DNA was amplified with strain-specific primers using a Rotor-Gene Q machine (Qiagen, NSW, Australia) (Table 1) ...
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bioRxiv - Microbiology 2021Quote: ... mixed with 0.4μM of sequencing primers and loaded into the PyroMark™ Q96 machine (Qiagen, Germany).
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bioRxiv - Immunology 2022Quote: ... primers followed by reverse phase 0.65 x SPRI beads purification and a QIAquick Spin Column (QIAGEN) purification ...
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bioRxiv - Microbiology 2022Quote: ... Primer design and sequence analysis were performed using the CLC Main workbench 21.0.3 (CLCbio, QIAGEN, Denmark). See table S9 for a list of oligonucleotides.
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bioRxiv - Molecular Biology 2024Quote: ... 2.8□l of forward and reverse primer at 5□M concentration and 10□l QuantiNova (Qiagen), made up to a final volume of 20□l with nuclease-free water ...
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bioRxiv - Genetics 2023Quote: ... Mouse Ahrr and Cyp1a1 primers were generated using the PyroMark Assay Design software version 2.0 (Qiagen). Primer sequences can be found in Table S1 ...