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Citations for Qiagen :
2201 - 2250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... which was further verified and purified from gel with a DNA purification kit (Qiagen, Cat.No. 28704). Then ...
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bioRxiv - Developmental Biology 2024Quote: gDNA extractions were done using Qiagen DNeasy Blood & Tissue Kit (Qiagen, Valencia, CA; Cat# 69506). PCR amplification was done using the following primers for targeted region ...
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bioRxiv - Developmental Biology 2024Quote: ... the final plasmids were isolated and purified using the Qiagen Plasmid Maxi kit (QIAGEN, Cat.No.12162). Subsequently ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Batches of samples were processed in parallel to collect RNA with Qiagen RNeasy extraction kits (Qiagen 74034). After isolating total RNA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... total RNA was extracted using Trizol (Qiagen) from i5 males (6-8 biological replicates per treatment ...
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bioRxiv - Developmental Biology 2024Quote: ... GFP-positive cells were sorted into REPLI-g cell storage buffer (Qiagen).
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bioRxiv - Developmental Biology 2024Quote: Genomic DNA from freshly sorted single cells was extracted and amplified using the REPLI-g Advanced Single Cell kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: Pools of 5 embryos were used for RNA extraction via the Qiagen RNeasy Micro Kit (Qiagen, Valencia CA; Cat# 74004). RNA was eluted in RNase-free water and sent to the New York University Genome Technology Center for RNA-sequencing ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA was extracted using the RNeasy plus Mini kit (QIAGEN, Cat.No. A25776). The quality of the RNA was measured using the Agilent 2100 Bioanalyser with the Agilent RNA 6000 Nano kit (Agilent ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were processed (Qiagen, 74104) and cytoplasmic and mitochondrial rRNA-deleted stranded Total RNA libraries were prepared using Illumina Truseq RNA Library Prep kit (Illumina 20020589 ...
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bioRxiv - Developmental Biology 2024Quote: ... and isolated total RNA using RNeasy mini kit (Qiagen, Germantown, MD, USA). We generated cDNA from 1 µg of RNA using the SuperScript IV First Stand Synthesis kit (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: Confluent cells were collected using accutase and total RNA was extracted using RNeasy microRNA isolation kit (Qiagen, Cat No. 74004), with the samples digested with RNase-free DNase I to eliminate genomic DNA ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by gel extraction (Qiagen).
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bioRxiv - Developmental Biology 2024Quote: ... Samples were loaded onto RNeasy Maxi Kit (Qiagen Cat# 75152) columns and the RNeasy protocol for RNA washes and elution was followed (RNeasy Midi/Maxi Handbook ...
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bioRxiv - Developmental Biology 2024Quote: ... reagent and RNA purification columns (Qiagen). Libraries were prepared with the KAPA mRNA hyperprep kit (Roche) ...
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bioRxiv - Developmental Biology 2024Quote: ... and was followed by gel extraction (Qiagen). Once libraries passed internal quality control they were sequenced on the Illumina platform.
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bioRxiv - Developmental Biology 2024Quote: ... using an 85 μm nozzle into 200 μL of RLT buffer (Qiagen, RNA mini kit). Single cell sequencing was done according to manufacturer’s instructions (10x genomics ...
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bioRxiv - Developmental Biology 2024Quote: ... with on-column DNase treatment (QIAGEN) as per manufacturer’s protocol.
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bioRxiv - Developmental Biology 2024Quote: ... Further disruption was carried out using QIAshredder columns (QIAGEN) and RNA was extracted using the RNeasy Mini Kit (QIAGEN ...
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bioRxiv - Developmental Biology 2024Quote: ... and RNA was extracted using the RNeasy Mini Kit (QIAGEN) with on-column DNase treatment (QIAGEN ...
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bioRxiv - Developmental Biology 2024Quote: ... collected into 96-well plates (on ice) prior to the addition of 10 μL of RLT plus lysis buffer (Qiagen, Hilden, Germany). All instruments and surfaces were cleaned with 80% v/v ethanol ...
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bioRxiv - Genetics 2024Quote: ... was ground to a fine powder in a liquid nitrogen pre-cooled mortar and RNA was extracted using the RNeasy Plant Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... primarily the Qiagen DNEasy Blood and Tissue kit (www.Qiagen.com) and the GenElute Mammalian Genomic DNA Miniprep kit (www.sigmaaldrich.com ...
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bioRxiv - Developmental Biology 2024Quote: Genomic DNA was extracted from tail tip-derived fibroblasts with a QIAamp DNA kit (Qiagen), treated with bisulfite using the EZ DNA Methylation-Direct kit (Zymo Research ...
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bioRxiv - Developmental Biology 2024Quote: ... and cleaned up using QIAquick PCR purification columns (Qiagen). The digested DNA was spiked with 0.01% control DNA duplexes ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA extraction was carried out using the Qiagen RNeasy micro kit per manufacturer’s instruction (Qiagen 74104). For RNA sequencing ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA extraction was performed using RNEasy Mini Kit (Qiagen cat. 74104) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... PCR was performed with 2 µL (∼16 ng) or 4 µL (∼32 ng) of BT-converted DNA with HotStar Taq Polymerase (Qiagen), depending on the genomic region ...
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bioRxiv - Genetics 2024Quote: ... PyroMark PCR Kit (Qiagen#978703) was used to amplify genomic fragments surrounding the sgRNA target regions from bisulfite converted DNA according to the company’s instructions ...
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bioRxiv - Genetics 2024Quote: ... The PCR amplicons were sequenced using PyroMark Q48 Advanced CpG Reagents (Qiagen#974022) based on manufactures instructions.
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bioRxiv - Genomics 2024Quote: ... Reactions were pooled and purified with the QIAquick PCR & Gel Cleanup Kit (Qiagen 28506). We then gel purified reactions with the QIAquick PCR & Gel Cleanup Kit (Qiagen 28506) ...
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bioRxiv - Genomics 2024Quote: ... Genomic DNA was purified with the QIAamp DNA Blood Midi Kit (Qiagen 51183). Libraries were then prepared for next-generation sequencing using a single-step PCR ...
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bioRxiv - Genomics 2024Quote: ... gel purified with the QIAquick PCR & Gel Cleanup Kit (Qiagen 28506), quantified with the Qubit High Sensitivity dsDNA Quantitation assay (Thermo Fisher Scientific Q32854) ...
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bioRxiv - Genomics 2024Quote: Generated sequence reads were imported into the Genomics Workbench software (version 21.0.4; Qiagen). The data analysis workflow was as follows ...
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bioRxiv - Genomics 2024Quote: ... Sixty microliters of RNA was extracted from 140 μl of each clinical specimen by using a Viral RNA Mini kit (Qiagen, Hilden, Germany) with the automated extraction platform QIAcube (Qiagen).
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bioRxiv - Genetics 2024Quote: ... and bound DNA was eluted using FG3 buffer (Qiagen). PCR amplification was performed with Phusion or Q5 High Fidelity DNA Polymerase (New England Biolabs) ...
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bioRxiv - Genetics 2024Quote: ... cDNA synthesis was performed using the QuantiTect Reverse Transcription kit (Qiagen). RT-qPCR was performed using KAPA SYBR® FAST qPCR Master Mix (2X ...
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bioRxiv - Genetics 2024Quote: ... each sample was lysed in a heating block with AL-Buffer (Qiagen) containing 0.2 mg/mL Proteinase K (Qiagen ...
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bioRxiv - Genetics 2024Quote: ... containing 0.2 mg/mL Proteinase K (Qiagen) for 10 minutes at 56°C ...
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bioRxiv - Genetics 2024Quote: ... The genomic DNA was extracted from thawed pellets using a DNeasy Blood & Tissue Kit (Qiagen, 69506) or JetFlex™ Genomic DNA Purification Kit (A30700 ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was extracted from approximately 5-10 million cells from each organ using the RNeasy Mini Kit (QIAGEN). SuperScript® IV Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... RNA was extracted using the RNeasy Micro kit (Qiagen) according to manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... 1 μl of the first round PCR product was used as a template for the second nested PCR reaction using HotStartTaq Master Mix Kit (QIAGEN). The cycling conditions for the second PCR reaction were 95°C for 5 min ...
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bioRxiv - Immunology 2024Quote: ... Immature (CD24high) thymocytes from both B6 and B6.SAP-/- mice were sorted directly into 350 microliters of RLT lysis buffer (Qiagen). RNA was extracted using the RNeasy Micro kit (Qiagen ...
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bioRxiv - Immunology 2024Quote: ... 25mg of tissue was dissociated using Bead Ruptor Elite (Omni International) for 45 seconds at 4.85m/s and RNA was isolated using RNeasy Fibrous Tissue Mini Kit 50 (Qiagen) following manufacturer’s indications ...
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bioRxiv - Genomics 2024Quote: ... The tissue sample was first ground into powder with liquid nitrogen and subsequently proceeded with the Qiagen MagAttract HMW kit (Qiagen Cat. No. 67563), following the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... and aligned to a human reference sequence GRCh38/hg38 by using CLC Genomics Workbench v20 (Qiagen). The TPM (transcript per million ...
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bioRxiv - Genetics 2024Quote: ... The DNA from the nested-PCR was purified using a Gel-extraction kit (Qiagen #28706). Subsequently ...
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bioRxiv - Microbiology 2024Quote: ... total RNAs were extracted using the RNeasy Plant Mini Kit (Qiagen) and DNase treated with RQ1 RNase-Free DNase (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... 10 Arabidopsis seedlings from the same MS plate were sampled together in 2 mL microtubes containing two 3 mm-diameter tungsten carbide beads (Qiagen), and flash frozen in liquid nitrogen ...