Labshake search
Citations for Qiagen :
2051 - 2100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and purified via PCR purification or gel extraction kit (both Qiagen). Vector and insert were ligated using T4 DNA ligase (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... 1 mL of culture (0′) was removed and mixed with 2 mL of RNAprotect Bacterial Reagent (QIAGEN), vortexed ...
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bioRxiv - Microbiology 2024Quote: ... and total RNA was extracted using the RNeasy Mini Kit protocol (Qiagen-ref 74104). Extracted total RNA was treated with Turbo DNAse (InvitrogenTM Ref ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were then purified with the QIAquick® PCR Purification Kit (Qiagen) and sequenced at Eurofins Genomics (Cologne ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were purified with the QIAprep Spin Miniprep Kit (QIAGEN, Hilden, Germany).
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bioRxiv - Microbiology 2024Quote: ... Plasmid DNA was extracted using QIAprep Spin Miniprep Kit (Qiagen, Germantown MD USA) and used to transform E ...
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bioRxiv - Microbiology 2024Quote: DNA extraction was performed using the DNeasy PowerSoil Pro Kit (Qiagen, Toronto, Canada) according to kit protocols ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using the DNeasy PowerSoil Pro Kit (Qiagen, Toronto, Canada) according to kit protocols ...
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bioRxiv - Microbiology 2024Quote: ... followed by rotation of the adapter and ten additional minutes of bead beating using a TissueLyser II (QIAGEN; Cat. No. 85300) using a 2 ml Tube Holder Set (QIAGEN ...
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bioRxiv - Microbiology 2024Quote: LAMP reactions (final volume of 25 µl) were performed in Rotor-Gene Q (Qiagen, Germatown, MD). The reaction mixture contained 15 µl of Optigene Master Mix (Optigene ...
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bioRxiv - Microbiology 2024Quote: ... The DNA was extracted using DNeasy Blood and Tissue Kit (Qiagen). The plant samples infected with Dickeya and/or Pectobacterium were confirmed using previously developed assay in our lab (Dobhal et al ...
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bioRxiv - Microbiology 2024Quote: ... the samples were homogenized using a bead beater (Qiagen Tissue Lyzer II), with the addition of lysozyme to improve the efficiency of nucleic acid extraction ...
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bioRxiv - Microbiology 2024Quote: ... The DNA was isolated using DNeasy Blood and Tissue Kit (Qiagen, Germantown, MA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The CLC Genomics Workbench 21 (Qiagen, Aarhus) with default settings was used to assemble the raw reads after trimming.
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bioRxiv - Microbiology 2024Quote: ... PCR products were visualized by capillary electrophoresis using a QIAxcel DNA high-resolution gel cartridge on a QIAxcel instrument (Qiagen, Toronto, Canada), according to manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... 24 and 44 hpi and DNA was extracted using DNAeasy blood and tissue kit (Qiagen). Genome copy number quantitation was done by quantitative polymerase chain reaction (qPCR ...
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bioRxiv - Microbiology 2024Quote: Bacterial genomic DNA was extracted separately from caecal tissue (∼100 mg) and caecal contents (∼200 mg) using a QIAamp Fast DNA Stool Mini kit (QIAGEN, Valencia, CA, USA) following the manufacturer’s pathogen detection protocol ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA were purified following manufacturer instruction for Gram-positive bacteria (DNeasy Blood and Tissue – Qiagen) and sequenced (Illumina sequencing at Core facility or Eurofins Genomics) ...
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bioRxiv - Microbiology 2024Quote: ... DNA extraction was performed utilizing commercial kits and following manufacturer’s protocol (Qiagen’s DNeasy 96 Blood & Tissue Kit, Qiagen). The amount and quality of extracted DNA was evaluated using PicoGreen fluorometer and NanoDrop spectrophotometer (ThermoFisher Scientific) ...
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Neutralizing gut-derived lipopolysaccharide as a novel therapeutic strategy for severe leptospirosisbioRxiv - Microbiology 2024Quote: ... PCR products were purified using the Qiagen Gel Extraction Kit (Qiagen, Germany). Sequencing libraries were generated using the SMRTbellTM Template Prep Kit (PacBio ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted from dogs’ feces using QIAamp PowerFecal Pro DNA Kit (Qiagen), following manufacturers’ instructions ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized from 500 ng of RNA using Quantitect Reverse Transcriptase kits (Qiagen) and qPCR was performed using perfeCTa SYBR green fastmix (Quanta Bioscience ...
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bioRxiv - Microbiology 2024Quote: ... Analysis of the RNAseq results was performed using CLC Genomic Workbench 23.0.1 (Qiagen). Reads were mapped to the reference genome of HG001 (NZ_CP018205.1) ...
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bioRxiv - Microbiology 2024Quote: ... DNA was isolated using the DNeasy Blood & Tissue kit (Qiagen) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... rpsL and qoxA transcripts by qRT‒PCR was performed with QuantiFast SYBR Green RT‒PCR Kit (Qiagen) using the Quantstudio3 system (Applied Biosystems) ...
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bioRxiv - Microbiology 2024Quote: ... Pathway analysis was conducted using Ingenuity Pathway Analysis (IPA, Qiagen) using genes with differential gene expression log2 fold change greater than |1.5|.
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bioRxiv - Microbiology 2024Quote: ... and total RNA was isolated using miRNeasy Mini Kits (Qiagen, CA, USA) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Pre-designed small interfering RNAs (siRNAs) and the non-target control (negative control) AllstarNeg were from Qiagen. 2×104 HeLa cells were reverse transfected with 10 nM siRNA using Lipofectamine RNAiMax (Life Technologies ...
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bioRxiv - Microbiology 2024Quote: RNA extraction was performed using RNeasy Kit (Qiagen) according to supplier’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA for some isolates was extracted using DNeasy Blood & Tissue Kit (Qiagen), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Bacteria are harvested by centrifugation (5 minutes, 4°C) and washed with 1 ml cold PBS containing RNA stabilization reagents (RNAprotect, Qiagen) before flash freezing and storage at -80°C ...
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bioRxiv - Microbiology 2024Quote: ... We performed PCR reactions using the Taq Polymerase Phusion® High-Fidelity PCR Master Mix with GC buffer and prepared them according to the manufacturer’s instructions (Qiagen, Hilder, Germany). PCR amplification steps involved an initial denaturing step for 30 sec at 98°C ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were purified using the QIAquick Gel Extraction Kit (Qiagen). Restriction enzymes and T4 DNA ligase were purchased from NEB ...
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bioRxiv - Microbiology 2024Quote: Depletion of rRNA (FastSelect Bacterial, Qiagen), libraries construction (TruSeq Stranded mRNA ...
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bioRxiv - Microbiology 2024Quote: ... DNA extraction was performed utilizing commercial kits and following manufacturer’s protocol (Qiagen’s DNeasy 96 Blood & Tissue Kit, Qiagen). The amount and quality of extracted DNA was evaluated using PicoGreen fluorometer and NanoDrop spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA of isolated colony were purified from 7.5 ml of culture (DNeasy Blood and Tissue – Qiagen) with an additional step of cell lysis with microbeads (Precellys Evolution) ...
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bioRxiv - Microbiology 2024Quote: ... coli using the QIAprep Spin Miniprep Kit (Qiagen). All plasmids made by PCR cloning were sequenced by Azenta ...
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bioRxiv - Microbiology 2024Quote: ... The DNA-free RNA was concentrated by MinElute Cleanup kit (Qiagen). The rRNA depletion ...
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bioRxiv - Microbiology 2024Quote: ... Ni-NTA slurry (Qiagen) (0.8 ml bed volume ...
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bioRxiv - Microbiology 2024Quote: ... The QIAamp DNA Mini and Fast Cycling kits (QIAGEN, UK) were used for DNA extraction and PCR amplification ...
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted using the RNeasy plus bacteria kit (Qiagen) according to manufacturer indications ...
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bioRxiv - Microbiology 2024Quote: ... Expression levels were measured by quantitative PCR using pre-designed primers (Qiagen) and SYBR green (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: ... Cells were harvested at the indicated times post-infection and total DNA was extracted using the DNeasy Blood & Tissue kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The QIAmp viral RNA mini kit (Qiagen, Hilden, Germany) was used for the extraction of viral DNA/RNA from the concentrated virome solution (Fig ...
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bioRxiv - Microbiology 2024Quote: ... and purified with RNeasy columns (Qiagen). Reverse transcription of mRNA to cDNA was carried out using the iScript™ cDNA Synthesis kit (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... and the reaction mix was purified using DNeasy Blood & Tissue Kits (Qiagen, Hilden, Germany) after RT with modifications ...
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bioRxiv - Microbiology 2024Quote: ... Fecal DNA was extracted using a modified PowerFecal Pro Kit (QIAGEN; Hilden ...
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bioRxiv - Microbiology 2024Quote: ... The products were washed multiple times with 80% ethanol and the dried pellet was resuspended in 32.5 µL of EB buffer (Qiagen, Venlo, The Netherlands), incubated for two minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... DEGs were subjected to Ingenuity Pathway Analysis (IPA, Qiagen) to identify immune-related canonical pathways and gene networks that are most activated between the FLU or FLU/OTV and Healthy pigs ...
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bioRxiv - Immunology 2024Quote: ... RNA was next extracted using the RNeasy Micro Kit (Qiagen) and submitted for library preparation and sequencing to the Genome Technology Access Center at the McDonnell Genome Institute at Washington University in St ...