Labshake search
Citations for Qiagen :
2101 - 2150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... followed by vigorous lysis at 30 Hz for five minutes using the TissueLyser II (Qiagen). Post-mechanical lysis ...
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bioRxiv - Immunology 2024Quote: ... RNeasy mini kit (Qiagen) was used to extract total RNA from kidney tissue according to the manufacturer’s specifications ...
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bioRxiv - Immunology 2024Quote: ... as described previously.21 Lymphocytes were then purified using MACS LS Columns per the manufacturer’s instructions and sorted on the iCyt Synergy (Sony) into Buffer RLT (Qiagen). RNA was next extracted using the RNeasy Micro Kit (Qiagen ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from T cells using the RNeasy Mini kit (#74104, Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Isolated immune subsets were subjected to RNA extraction using an Rneasy micro kit (Qiagen) including treatment with Dnase I to remove genomic DNA ...
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bioRxiv - Immunology 2024Quote: ... column purified (Qiagen), and analyzed by 100 bp paired-end mRNA-sequencing using the BGISEQ-500 platform (BGIAmericasCorp ...
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bioRxiv - Immunology 2024Quote: ... Genomic DNA from sorted cells was collected using DNeasy Blood & Tissue Kits (Qiagen, Cat. No. 69504). The sgRNA target sites were amplified using PCR and purified using the DNA clean and concentrate kit (Zymo Research ...
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bioRxiv - Immunology 2024Quote: ... tumor protein extracts were generated from snap-frozen biopsies using a combination of TissueLyser LT (Qiagen, Hilden, Germany) and four rounds of freeze/thaw cycling ...
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bioRxiv - Microbiology 2024Quote: ... The HotStartTaq® Plus Master Mix Kit (QIAGEN, Germany) was used for PCR and the amplification cycle included initial denaturation at 95°C for 5 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Microbial DNA was isolated with the QIAamp Fast DNA Stool Mini Kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... but not on BHIY/Thia plates were selected and DNA was isolated using a DNeasy blood and tissue kit (Qiagen) to investigate whether the intended chromosomal mutation was introduced ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was extracted from 250 mg soil using DNeasy PowerSoil kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted from TSB cultures following the manufacturer’s instructions using the DNeasy Blood and Tissue kit (Qiagen, Carlsbad, CA). Purified DNA was quantified ...
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bioRxiv - Microbiology 2024Quote: ... incorporating the QIAamp Fast DNA Stool Mini Kit (QIAGEN GmbH, Hilden, Germany) for DNA extraction ...
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bioRxiv - Immunology 2024Quote: ... Genomic DNA from the pooled recombinants and parent strain were isolated using the Gentra® Puregene® DNA isolation kit (Qiagen) and sent for whole genome sequencing (BGI) ...
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bioRxiv - Immunology 2024Quote: ... DNA from the blood of Plasmodium chabaudi infected mice was isolated from QIAmp DNA blood kit (QIAGEN, Valencia, CA) as per the manufacturer instruction ...
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bioRxiv - Immunology 2024Quote: ... followed by RNA extraction using RNeasy Mini Kit (Qiagen, Germantown, MD) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: The DNA was extracted from the FACS sorted cells and whole genomes were amplified using multiple displacement amplification technique (MDA) with the REPLI-g Single Cell kit (Qiagen) by following the manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... aeruginosa PA14 using the RNAeasy kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... with the addition of DNase (Qiagen) and lysozyme (Sigma Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... with Power Soil kits (Qiagen) as described previously (19,41) ...
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bioRxiv - Immunology 2024Quote: ... KPctrl and KP KO cell lines using DNA Blood and cell culture kit (Qiagen) following the manufactureŕs instructions ...
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bioRxiv - Immunology 2024Quote: ... cells pelleted and lysed in RLT buffer (Qiagen). RNA was extracted using Qiagen RNeasy micro kit and their transcriptome measured using the nanostring Human Immunology V2 characterisation panel (Nanostring technologies ...
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bioRxiv - Immunology 2024Quote: ... Illumina compatible libraries were generated using the QIAseq FX Library Kit (Qiagen, #180477). Sequencing runs were performed on the Illumina NextSeq1000 platform in the MMF using the 2×150 paired ends reads cassette ...
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bioRxiv - Immunology 2024Quote: ... Proteins were purified from filtered supernatants using nickel nitriloacetic acid (Ni-NTA) agarose (QIAGEN) via gravity flow ...
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bioRxiv - Immunology 2024Quote: ... DNA was extracted from CRISPR edited cells using DNsasy Blood & Tissue Kit (QIAGEN, 69506). DNA samples were PCR amplified using KOD Hot Start DNA Polymerase (MilliporeSigma ...
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bioRxiv - Microbiology 2024Quote: ... then total RNA was extracted with the RNeasy Mini kit (Qiagen), treated with DNase I (NEB ...
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bioRxiv - Microbiology 2024Quote: Total RNA was extracted by RNeasy kit (QIAGEN), and cDNA was synthesized by Verso reverse transcription (Thermo Fisher Scientic ...
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bioRxiv - Microbiology 2024Quote: ... uniform genome amplification (WGA) and transcriptome amplification (WTA) were performed using the repi-g Cell WGA and WTA Kit (150052, Qiagen, Hilden, Germany), based on the multiple displacement amplification (MDA ...
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bioRxiv - Microbiology 2024Quote: ... Flag-SHOC2 was cleaved from 6X-His-MBP by incubating 37 mg purified protein with 0.65 mg TEV protease at 4°C overnight followed by affinity purification using Nickel affinity resin (Qiagen) where cleaved Flag-SHOC2 was collected in the flow-through.
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Intratumoral Microbiome of Adenoid Cystic Carcinomas and Comparison with other Head and Neck CancersbioRxiv - Microbiology 2024Quote: ... Microbial genomic DNA was extracted using the DNeasy Powersoil Pro DNA kit (Cat No. 47014, QIAGEN), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: DNA of bacterial isolates was extracted for WGS using the Qiagen DNeasy blood and tissue kit (Qiagen Co., Germany), manipulated according to the protocol provided by the manufacturer.
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bioRxiv - Microbiology 2024Quote: DNA extraction was performed with PowerSoil kit (QIAGEN, Germantown, MD), eluted with 100 μl of the provided elution solution ...
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bioRxiv - Microbiology 2024Quote: ... The fusion protein was affinity purified using Ni-NTA agarose (Qiagen; Table S4), eluted with 50 mM Tris-base ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from RPE/choroid complex and primary RPE cells using a RNA extraction kit (RNeasy plus mini kit QIAGEN). The RNA concentration and integrity were determined with NanoDrop 1000 spectrophotometer ...
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bioRxiv - Microbiology 2024Quote: ... total genomic DNA (gDNA) was extracted using the DNeasy Blood & Tissue Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... A total of 500 ng of RNA was used to synthesize cDNA using the QuantiTech reverse transcription kit (Qiagen). The cDNA samples were then diluted 1:10 and used as a template to perform RT-qPCR ...
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bioRxiv - Microbiology 2024Quote: ... The incubated resin was loaded into 5 ml polypropylene column (Qiagen) subsequently multiple washes were applied using a wash buffer pH 8.0 (50 mM NaH2PO4 ...
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bioRxiv - Microbiology 2024Quote: ... Soluble protein fractions were then mixed with pre-equilibrated Ni-NTA agarose resin (Qiagen) and incubated at 4°C for 1 h with rotation ...
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bioRxiv - Microbiology 2024Quote: ... Pooled RNA was further purified using the RNeasy MinElute clean-up kit (Qiagen, Hilden, Germany) and quantified using the Qubit RNA Broad-range Assay with the Qubit Fluorometer v3.0 (Thermofisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted with the RNA Powersoil Total RNA kit from Qiagen.
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bioRxiv - Microbiology 2024Quote: ... The fusion protein was affinity purified using Ni-NTA agarose (Qiagen), eluted with 50 mM Tris-base ...
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bioRxiv - Microbiology 2024Quote: ... The PCR sample was then digested with DpnI to cut the residual template DNA and purified with the Pure Link PCR purification kit (Qiagen, Hilden, Germany). The DNA fragment was labeled with 5-(3-aminoallyl)dUTP by nick translation ...
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bioRxiv - Microbiology 2024Quote: ... the procedure was repeated using another DNA extraction method – DNeasy Blood & Tissue Kit (Qiagen Ltd.), a standard kit in invertebrate microbiota studies ...
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bioRxiv - Microbiology 2024Quote: ... and the amplicon pool was cleaned with the QIAquick PCR Purification Kit (Qiagen 28104). If spurious bands were identified the 478 bp fragment was extracted using the QIAquick Gel Extraction Kit (Qiagen 28704 ...
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bioRxiv - Microbiology 2024Quote: High molecular weight genomic DNA was prepared using the DNeasy Blood and Tissue kit (QIAgen), according to the manufacturer’s instruction for Gram positive bacteria ...
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bioRxiv - Microbiology 2024Quote: ... or if more than one band was present extracted from 1% TAE gel using the QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany). Nucleotide BLAST (BLASTn ...
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bioRxiv - Microbiology 2024Quote: ... GFP expressing cells were directly sorted into buffer RLT (Qiagen) containing β-mercaptoethanol ...
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bioRxiv - Microbiology 2024Quote: ... RNAs were extracted using the RNeasy Mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted using RNeasy Plus Mini kit (QIAGEN) and subsequently treated with Turbo DNA-free Kit (Ambion) ...